Restriction map for
pFN21AB6488
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
AocI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BalI BamHI BanII BbeI BbrPI
BbvCI BciVI BclI BfrI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DsaI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 135 GACGCC G[GA]^CG[CT]C
Alw44I 390 GTGCAC G^TGCAC
AlwI 512 GGATC GGATC(4/5)
Aor51HI 98 AGCGCT AGC^GCT
ApaLI 390 GTGCAC G^TGCAC
AvrII 582 CCTAGG C^CTAGG
BanI 486 GGTGCC G^G[CT][GA]CC
BbsI 1050 GAAGAC GAAGAC(2/6)
BcgI 34 CGAAAGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 583 CTAG C^TAG
BglI 206 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 228 AGATCT A^GATCT
BlnI 582 CCTAGG C^CTAGG
BpuAI 1050 GAAGAC GAAGAC(2/6)
BsaHI 135 GACGCC G[GA]^CG[CT]C
BseRI 529 GAGGAG GAGGAG(10/8)
BssHII 162 GCGCGC G^CGCGC
BssSI 18 CACGAG CACGAG(-5/-1)
BstAPI 3 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 681 GGTCACC G^GT[ACGT]ACC
BstPI 681 GGTCACC G^GT[ACGT]ACC
DraII 985 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 630 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 601 GACTCAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 891 TGGCCG [CT]^GGCC[GA]
Eco47III 98 AGCGCT AGC^GCT
EcoNI 75 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 985 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 681 GGTCACC G^GT[ACGT]ACC
EcoT14I 582 CCTAGG C^C[AT][AT]GG
HaeII 98 AGCGCT [GA]GCGC^[CT]
HgaI 135 GACGC GACGC(5/10)
Hin1I 135 GACGCC G[GA]^CG[CT]C
HincII 610 GTCAAC GT[CT]^[GA]AC
HindII 610 GTCAAC GT[CT]^[GA]AC
HphI 683 TCACC GGTGA(8/7)
MaeI 583 CTAG C^TAG
MboII 1050 GAAGA GAAGA(8/7)
NspI 643 GCATGC [GA]CATG^[CT]
PpuMI 985 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 433 CAGCTG CAG^CTG
SmlI 603 CTCAAG C^T[CT][GA]AG
SphI 643 GCATGC GCATG^C
StyI 582 CCTAGG C^C[AT][AT]GG
TaqI 335 TCGA T^CGA
TfiI 535 GATTC G^A[AT]TC
TthHB8I 335 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcsI 364 AAATTT [GA]^AATT[CT]
382 AAATTC
AlwNI 867 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1036 CAGGAGCTG
ApoI 364 AAATTT [GA]^AATT[CT]
382 AAATTC
AvaII 279 GGACC G^G[AT]CC
986 GGACC
BsmFI 78 GGGAC GGGAC(10/14)
985 GGGAC
BstYI 228 AGATCT [GA]^GATC[CT]
512 GGATCT
FauI 203 CCCGC CCCGC(4/6)
425 CCCGC
MflI 228 AGATCT [GA]^GATC[CT]
512 GGATCT
SfaNI 708 GATGC GCATC(5/9)
299 GCATC
StuI 546 AGGCCT AGG^CCT
1025 AGGCCT
Tsp509I 365 AATT ^AATT
383 AATT
XhoII 228 AGATCT [GA]^GATC[CT]
512 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PstI Sau3AI Sau96I
ScrFI SfcI SmaI TseI Tsp45I
TspRI Van91I XmaCI XmaI