(Circular) (Six-base) MAPSORT of: pFN21AB6488.seq Check: 8723 from: 1 to: 5776 pFN21AB6488 5776 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2408 2838 2408 Size: 430 5346 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3074 3839 5223 5491 1632 Size: 1442 765 1384 268 1917 Fragments arranged by size: 1917 1442 1384 765 268 AfeI AGC'GCT Cuts at: 2105 2105 Size: 5776 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2870 1184 Size: 1686 4090 AlwNI CAG_nnn'CTG Cuts at: 2877 3046 3519 5091 2877 Size: 169 473 1572 3562 Fragments arranged by size: 3562 1572 473 169 ApaLI G'TGCA_C Cuts at: 2395 3833 4989 2395 Size: 1438 1156 3182 Fragments arranged by size: 3182 1438 1156 ApoI r'AATT_y Cuts at: 1493 2369 2387 3085 3311 3463 5572 1493 Size: 876 18 698 226 152 2109 1697 Fragments arranged by size: 2109 1697 876 698 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2122 2159 2682 3101 1870 Size: 73 179 37 523 419 4545 Fragments arranged by size: 4545 523 419 179 73 37 AvrII C'CTAG_G Cuts at: 2587 2587 Size: 5776 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2491 3097 4552 618 Size: 325 217 627 704 606 1455 1842 Fragments arranged by size: 1842 1455 704 627 606 325 217 BanII G_rGCy'C Cuts at: 1391 3095 1391 Size: 1704 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3062 928 Size: 971 1163 3642 Fragments arranged by size: 3642 1163 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2028 2062 3967 4001 1903 Size: 34 50 34 7 34 1905 34 3678 Fragments arranged by size: 3678 1905 50 34 34 34 34 7 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2217 4387 136 Size: 107 122 71 631 605 545 2170 1525 Fragments arranged by size: 2170 1525 631 605 545 122 107 71 BglII A'GATC_T Cuts at: 2233 5771 2233 Size: 3538 2238 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2123 2135 2209 2399 2430 3837 4993 1790 Size: 333 12 74 190 31 1407 1156 2573 Fragments arranged by size: 2573 1407 1156 333 190 74 31 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2110 2555 3507 Size: 416 53 83 399 24 693 445 952 Cuts at: 3507 4465 442 Size: 958 1753 Fragments arranged by size: 1753 958 952 693 445 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2245 2245 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 2517 3023 3068 4436 1239 Size: 1019 259 506 45 1368 2579 Fragments arranged by size: 2579 1368 1019 506 259 45 Bpu10I CC'TnA_GC Cuts at: 2457 2457 Size: 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2593 3901 4766 5064 2593 Size: 1308 865 298 3305 Fragments arranged by size: 3305 1308 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2141 3583 3965 4600 Size: 53 83 186 936 608 1442 382 635 Cuts at: 4600 275 Size: 1451 Fragments arranged by size: 1451 1442 936 635 608 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4607 4881 5028 5439 4209 Size: 398 274 147 411 4546 Fragments arranged by size: 4546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2583 2613 1178 Size: 30 165 30 1180 30 4341 Fragments arranged by size: 4341 1180 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2549 1186 Size: 1363 4413 BseYI C'CCAG_C Cuts at: 2288 2470 4979 5475 2288 Size: 182 2509 496 2589 Fragments arranged by size: 2589 2509 496 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 2293 2830 2840 2890 2980 1163 Size: 573 300 257 537 10 50 90 3959 Fragments arranged by size: 3959 573 537 300 257 90 50 10 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 2399 2951 3837 3922 4993 2024 Size: 375 552 886 85 1071 2807 Fragments arranged by size: 2807 1071 886 552 375 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2123 2135 2209 2399 2430 2951 Size: 399 234 99 12 74 190 31 521 Cuts at: 2951 3095 3837 3922 4993 1391 Size: 144 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 521 399 234 190 144 99 85 74 31 12 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2159 2408 2838 3002 3131 844 Size: 1315 249 430 164 129 3489 Fragments arranged by size: 3489 1315 430 249 164 129 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 2167 1925 Size: 242 5534 BssSI C'ACGA_G Cuts at: 2023 3836 4848 2023 Size: 1813 1012 2951 Fragments arranged by size: 2951 1813 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2014 794 Size: 1220 4556 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 2686 1184 Size: 1502 4274 BstYI r'GATC_y Cuts at: 1972 2233 2517 3106 3473 3858 3875 5327 5413 Size: 261 284 589 367 385 17 1452 86 Cuts at: 5413 5425 5771 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 589 385 367 346 284 261 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2051 2263 2365 2896 3292 4086 4106 2051 Size: 212 102 531 396 794 20 3721 Fragments arranged by size: 3721 794 531 396 212 102 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2644 2679 529 Size: 35 2080 35 3626 Fragments arranged by size: 3626 2080 35 35 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DraIII CAC_nnn'GTG Cuts at: 2640 2640 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2612 4783 809 Size: 507 1296 2171 1802 Fragments arranged by size: 2171 1802 1296 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2896 3223 4112 Size: 54 1425 50 275 18 1066 327 889 Cuts at: 4112 5741 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1066 889 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3706 1353 Size: 2353 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2517 3023 3068 3074 3839 4436 Size: 393 626 259 506 45 6 765 597 Cuts at: 4436 5223 5491 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 765 626 597 506 393 268 259 45 6 EcoNI CCTnn'n_nnAGG Cuts at: 2084 2084 Size: 5776 EcoO109I rG'GnC_Cy Cuts at: 2991 2991 Size: 5776 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2107 4923 1678 Size: 429 2816 2531 Fragments arranged by size: 2816 2531 429 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2017 2049 2171 2203 2583 Size: 32 135 32 445 32 122 32 380 Cuts at: 2583 2615 4417 4449 4491 4523 1373 Size: 32 1802 32 42 32 2626 Fragments arranged by size: 2626 1802 445 380 135 122 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2617 3120 3366 669 Size: 988 281 679 503 246 3079 Fragments arranged by size: 3079 988 679 503 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4890 5074 1305 Size: 3585 184 2007 Fragments arranged by size: 3585 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3954 Fragments arranged by size: 3954 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3734 4093 4252 518 Size: 648 2568 359 159 2042 Fragments arranged by size: 2568 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2433 2440 2901 3192 3869 5017 5262 1606 Size: 827 7 461 291 677 1148 245 2120 Fragments arranged by size: 2120 1148 827 677 461 291 245 7 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5224 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2652 3134 4679 1132 Size: 97 1423 482 1545 2229 Fragments arranged by size: 2229 1545 1423 482 97 PasI CC'CwG_GG Cuts at: 1348 2954 1348 Size: 1606 4170 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 2080 2640 3046 1108 Size: 972 560 406 3838 Fragments arranged by size: 3838 972 560 406 PfoI T'CCnGG_A Cuts at: 3039 3039 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2991 2991 Size: 5776 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PstI C_TGCA'G Cuts at: 830 2405 2835 2891 3128 830 Size: 1575 430 56 237 3478 Fragments arranged by size: 3478 1575 430 237 56 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2440 2440 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SfcI C'TryA_G Cuts at: 826 1045 2401 2831 2887 3124 4259 4940 5131 Size: 219 1356 430 56 237 1135 681 191 Cuts at: 5131 826 Size: 1471 Fragments arranged by size: 1471 1356 1135 681 430 237 219 191 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 2124 2161 2684 3103 1872 Size: 252 37 523 419 4545 Fragments arranged by size: 4545 523 419 252 37 SmlI C'TyrA_G Cuts at: 820 1017 1943 2608 3880 4781 5043 820 Size: 197 926 665 1272 901 262 1553 Fragments arranged by size: 1553 1272 926 901 665 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 2652 3134 1132 Size: 1520 482 3774 Fragments arranged by size: 3774 1520 482 SrfI GCCC'GGGC Cuts at: 2124 2124 Size: 5776 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 2553 3032 1517 Size: 1036 479 4261 Fragments arranged by size: 4261 1036 479 StyI C'CwwG_G Cuts at: 513 1065 2587 3215 513 Size: 552 1522 628 3074 Fragments arranged by size: 3074 1522 628 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3803 3820 3973 4158 4603 325 Size: 1255 2223 17 153 185 445 1498 Fragments arranged by size: 2223 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5734 96 Size: 274 80 33 51 1443 2045 1712 138 Fragments arranged by size: 2045 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2998 3030 5736 5768 2998 Size: 32 2706 32 3006 Fragments arranged by size: 3006 2706 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3070 3070 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 2122 2159 2682 3101 1870 Size: 252 37 523 419 4545 Fragments arranged by size: 4545 523 419 252 37 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI