(Circular) (Six-base) MAPSORT of: pFN21AE1067.seq Check: 2729 from: 1 to: 6223 pFN21AE1067 6223 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3058 3058 Size: 6223 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4033 278 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3566 1937 Size: 1629 4594 Acc65I G'GTAC_C Cuts at: 3544 3544 Size: 6223 AclI AA'CG_TT Cuts at: 1563 4350 4723 1563 Size: 2787 373 3063 Fragments arranged by size: 3063 2787 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2279 2374 2410 2975 3107 4286 5670 5938 Size: 647 95 36 565 132 1179 1384 268 Cuts at: 5938 1632 Size: 1917 Fragments arranged by size: 1917 1384 1179 647 565 268 132 95 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6026 AflIII A'CryG_T Cuts at: 1203 5122 6099 1203 Size: 3919 977 1327 Fragments arranged by size: 3919 1327 977 AgeI A'CCGG_T Cuts at: 5054 5054 Size: 6223 AhdI GACnn_n'nnGTC Cuts at: 3439 4952 3439 Size: 1513 4710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6223 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2556 1184 Size: 1372 4851 AlwNI CAG_nnn'CTG Cuts at: 3490 3966 5538 3490 Size: 476 1572 4175 Fragments arranged by size: 4175 1572 476 ApaLI G'TGCA_C Cuts at: 4280 5436 4280 Size: 1156 5067 ApoI r'AATT_y Cuts at: 1493 2699 3532 3758 3910 6019 1493 Size: 1206 833 226 152 2109 1697 Fragments arranged by size: 2109 1697 1206 833 226 152 AseI AT'TA_AT Cuts at: 160 1032 4777 160 Size: 872 3745 1606 Fragments arranged by size: 3745 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 AvaI C'yCGr_G Cuts at: 1870 1943 2403 3311 3548 1870 Size: 73 460 908 237 4545 Fragments arranged by size: 4545 908 460 237 73 BamHI G'GATC_C Cuts at: 2055 3553 3920 2055 Size: 1498 367 4358 Fragments arranged by size: 4358 1498 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2411 3544 4999 618 Size: 325 217 627 624 1133 1455 1842 Fragments arranged by size: 1842 1455 1133 627 624 325 217 BanII G_rGCy'C Cuts at: 1391 2787 3116 3157 3366 3542 1391 Size: 1396 329 41 209 176 4072 Fragments arranged by size: 4072 1396 329 209 176 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5252 BbvCI CC'TCA_GC Cuts at: 2973 2973 Size: 6223 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4414 4448 1903 Size: 34 50 34 2393 34 3678 Fragments arranged by size: 3678 2393 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2169 4115 5325 2169 Size: 1946 1210 3067 Fragments arranged by size: 3067 1946 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6223 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2624 2633 4834 Size: 107 122 71 631 605 952 9 2201 Cuts at: 4834 136 Size: 1525 Fragments arranged by size: 2201 1525 952 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 2385 6218 2385 Size: 3833 2390 BlpI GC'TnA_GC Cuts at: 2516 3640 2516 Size: 1124 5099 Bme1580I G_kGCm'C Cuts at: 1790 2046 2852 4284 5440 1790 Size: 256 806 1432 1156 2573 Fragments arranged by size: 2573 1432 1156 806 256 BmgBI CAC'GTC Cuts at: 1318 2128 2304 1318 Size: 810 176 5237 Fragments arranged by size: 5237 810 176 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3500 3954 4912 Size: 416 53 83 399 24 2083 454 958 Cuts at: 4912 442 Size: 1753 Fragments arranged by size: 2083 1753 958 454 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6223 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2644 3451 2644 Size: 807 5416 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2634 2823 2876 2888 3011 3026 3152 3308 Size: 1395 189 53 12 123 15 126 156 Cuts at: 3308 3380 4883 1239 Size: 72 1503 2579 Fragments arranged by size: 2579 1503 1395 189 156 126 123 72 53 15 12 Bpu10I CC'TnA_GC Cuts at: 2587 2973 3186 2587 Size: 386 213 5624 Fragments arranged by size: 5624 386 213 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4348 5213 5511 4348 Size: 865 298 5060 Fragments arranged by size: 5060 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2586 4886 882 Size: 617 1087 2300 2219 Fragments arranged by size: 2300 2219 1087 617 BsaAI yAC'GTr Cuts at: 493 1204 2113 4014 493 Size: 711 909 1901 2702 Fragments arranged by size: 2702 1901 909 711 BsaBI GATnn'nnATC Cuts at: 6217 6217 Size: 6223 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 4030 4412 5047 Size: 53 83 186 936 543 1954 382 635 Cuts at: 5047 275 Size: 1451 Fragments arranged by size: 1954 1451 936 635 543 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3149 4656 5054 5328 5475 5886 3149 Size: 1507 398 274 147 411 3486 Fragments arranged by size: 3486 1507 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2235 2265 2744 2774 3389 Size: 30 165 30 832 30 479 30 615 Cuts at: 3389 3419 1178 Size: 30 3982 Fragments arranged by size: 3982 832 615 479 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2660 2879 2891 3440 3464 1186 Size: 1474 219 12 549 24 3945 Fragments arranged by size: 3945 1474 549 219 24 12 BseYI C'CCAG_C Cuts at: 3214 3328 5426 5922 3214 Size: 114 2098 496 3515 Fragments arranged by size: 3515 2098 496 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5650 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3673 4434 5462 1540 Size: 275 186 1672 761 1028 2301 Fragments arranged by size: 2301 1672 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3168 3366 4284 4369 5440 3168 Size: 198 918 85 1071 3951 Fragments arranged by size: 3951 1071 918 198 85 BsmI GAATG_Cn' Cuts at: 2545 3202 3734 3827 2545 Size: 657 532 93 4941 Fragments arranged by size: 4941 657 532 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2787 2852 3116 3157 3168 3366 Size: 399 256 741 65 264 41 11 198 Cuts at: 3366 3542 4284 4369 5440 1391 Size: 176 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 741 399 264 256 198 176 85 65 41 11 BspEI T'CCGG_A Cuts at: 3149 5886 3149 Size: 2737 3486 BspHI T'CATG_A Cuts at: 2365 4112 5842 2365 Size: 1747 1730 2746 Fragments arranged by size: 2746 1747 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2058 3058 3207 3578 844 Size: 1214 1000 149 371 3489 Fragments arranged by size: 3489 1214 1000 371 149 BsrBI CCG'CTC Cuts at: 1394 1823 3147 4110 1394 Size: 429 1324 963 3507 Fragments arranged by size: 3507 1324 963 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4718 4892 65 Size: 1183 3470 174 1396 Fragments arranged by size: 3470 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4867 5054 1700 Size: 110 3057 187 2869 Fragments arranged by size: 3057 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6223 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6223 BssSI C'ACGA_G Cuts at: 3436 4283 5295 3436 Size: 847 1012 4364 Fragments arranged by size: 4364 1012 847 BstAPI GCAn_nnn'nTGC Cuts at: 794 2050 794 Size: 1256 4967 BstBI TT'CG_AA Cuts at: 5027 5027 Size: 6223 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6223 BstXI CCAn_nnnn'nTGG Cuts at: 2558 2612 2558 Size: 54 6169 BstYI r'GATC_y Cuts at: 1972 2055 2349 2385 3553 3920 4305 4322 5774 Size: 83 294 36 1168 367 385 17 1452 Cuts at: 5774 5860 5872 6218 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1168 385 367 346 294 86 83 36 17 12 Bsu36I CC'TnA_GG Cuts at: 3259 3259 Size: 6223 BtgI C'CryG_G Cuts at: 513 1065 2631 3108 3519 513 Size: 552 1566 477 411 3217 Fragments arranged by size: 3217 1566 552 477 411 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3373 487 Size: 683 56 2147 3337 Fragments arranged by size: 3337 2147 683 56 BtsI GCAGTG_nn' Cuts at: 2637 3739 4533 4553 2637 Size: 1102 794 20 4307 Fragments arranged by size: 4307 1102 794 20 ClaI AT'CG_AT Cuts at: 1554 2658 1554 Size: 1104 5119 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6188 DraI TTT'AAA Cuts at: 2191 2443 3527 2191 Size: 252 1084 4887 Fragments arranged by size: 4887 1084 252 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2863 5230 809 Size: 507 1547 2367 1802 Fragments arranged by size: 2367 1802 1547 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2548 2634 3670 Size: 54 1425 50 275 18 718 86 1036 Cuts at: 3670 4559 6188 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1036 889 718 275 86 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3670 1537 Size: 275 1858 4090 Fragments arranged by size: 4090 1858 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2450 4153 1353 Size: 1097 1703 3423 Fragments arranged by size: 3423 1703 1097 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3297 4810 5184 5330 387 Size: 1064 138 1708 1513 374 146 1280 Fragments arranged by size: 1708 1513 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3364 3364 Size: 6223 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2374 2410 2634 2823 2876 2888 Size: 393 647 95 36 224 189 53 12 Cuts at: 2888 2975 3011 3026 3107 3152 3308 3380 4286 Size: 87 36 15 81 45 156 72 906 Cuts at: 4286 4883 5670 5938 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 906 787 647 597 393 268 224 189 156 95 87 81 72 53 45 36 36 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 2803 3262 2803 Size: 459 5764 EcoO109I rG'GnC_Cy Cuts at: 2335 2625 2671 3196 2335 Size: 290 46 525 5362 Fragments arranged by size: 5362 525 290 46 EcoRI G'AATT_C Cuts at: 2699 3532 3910 2699 Size: 833 378 5012 Fragments arranged by size: 5012 833 378 FspI TGC'GCA Cuts at: 4729 4729 Size: 6223 HaeII r_GCGC'y Cuts at: 1678 3347 5370 1678 Size: 1669 2023 2531 Fragments arranged by size: 2531 2023 1669 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3389 3421 3425 3457 4864 Size: 32 135 32 1817 32 4 32 1407 Cuts at: 4864 4896 4938 4970 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1817 1407 135 42 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 3567 3813 669 Size: 988 281 1629 246 3079 Fragments arranged by size: 3079 1629 988 281 246 HindIII A'AGCT_T Cuts at: 748 2273 748 Size: 1525 4698 HpaI GTT'AAC Cuts at: 3813 3813 Size: 6223 KpnI G_GTAC'C Cuts at: 3548 3548 Size: 6223 MfeI C'AATT_G Cuts at: 3822 3822 Size: 6223 MluI A'CGCG_T Cuts at: 6099 6099 Size: 6223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3432 5337 5521 1305 Size: 2127 1905 184 2007 Fragments arranged by size: 2127 2007 1905 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2550 10 Size: 54 1425 343 718 3683 Fragments arranged by size: 3683 1425 718 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4181 4540 4699 518 Size: 648 3015 359 159 2042 Fragments arranged by size: 3015 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2863 3214 3639 4316 5464 5709 1606 Size: 1257 351 425 677 1148 245 2120 Fragments arranged by size: 2120 1257 1148 677 425 351 245 NcoI C'CATG_G Cuts at: 513 1065 3108 513 Size: 552 2043 3628 Fragments arranged by size: 3628 2043 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6223 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6223 NotI GC'GGCC_GC Cuts at: 3670 3670 Size: 6223 NspI r_CATG'y Cuts at: 1132 1229 3040 3581 5126 1132 Size: 97 1811 541 1545 2229 Fragments arranged by size: 2229 1811 1545 541 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6223 PciI A'CATG_T Cuts at: 5122 5122 Size: 6223 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6223 PfoI T'CCnGG_A Cuts at: 2694 2694 Size: 6223 PmeI GTTT'AAAC Cuts at: 3527 3527 Size: 6223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4292 4324 5805 5837 4292 Size: 32 1481 32 4678 Fragments arranged by size: 4678 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2335 2671 3196 2335 Size: 336 525 5362 Fragments arranged by size: 5362 525 336 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6223 PsiI TTA'TAA Cuts at: 3793 3793 Size: 6223 PstI C_TGCA'G Cuts at: 830 2526 2644 3027 3480 3575 830 Size: 1696 118 383 453 95 3478 Fragments arranged by size: 3478 1696 453 383 118 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6223 SacI G_AGCT'C Cuts at: 3366 3366 Size: 6223 SalI G'TCGA_C Cuts at: 1936 3565 1936 Size: 1629 4594 SapI GCTCTTCn'nnn_ Cuts at: 2450 2450 Size: 6223 SbfI CC_TGCA'GG Cuts at: 3575 3575 Size: 6223 ScaI AGT'ACT Cuts at: 4471 6183 4471 Size: 1712 4511 SfcI C'TryA_G Cuts at: 826 1045 2344 2522 2640 2730 3023 3476 3571 Size: 219 1299 178 118 90 293 453 95 Cuts at: 3571 4706 5387 5578 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1299 1135 681 453 293 219 191 178 118 95 90 SfiI GGCCn_nnn'nGGCC Cuts at: 2633 2633 Size: 6223 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6223 SmaI CCC'GGG Cuts at: 1872 3550 1872 Size: 1678 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2403 4327 5228 5490 820 Size: 197 926 460 1924 901 262 1553 Fragments arranged by size: 1924 1553 926 901 460 262 197 SnaBI TAC'GTA Cuts at: 493 2113 4014 493 Size: 1620 1901 2702 Fragments arranged by size: 2702 1901 1620 SpeI A'CTAG_T Cuts at: 152 152 Size: 6223 SphI G_CATG'C Cuts at: 1132 3581 1132 Size: 2449 3774 SspI AAT'ATT Cuts at: 5 52 4147 5 Size: 47 4095 2081 Fragments arranged by size: 4095 2081 47 StuI AGG'CCT Cuts at: 1517 2495 2844 1517 Size: 978 349 4896 Fragments arranged by size: 4896 978 349 StyI C'CwwG_G Cuts at: 513 1065 2132 2763 3108 3662 513 Size: 552 1067 631 345 554 3074 Fragments arranged by size: 3074 1067 631 554 552 345 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2818 4250 4267 4420 4605 5050 325 Size: 1255 1238 1432 17 153 185 445 1498 Fragments arranged by size: 1498 1432 1255 1238 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2022 4469 6181 Size: 274 80 33 51 1443 45 2447 1712 Cuts at: 6181 96 Size: 138 Fragments arranged by size: 2447 1712 1443 274 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2235 2267 2288 2320 2744 2776 6183 6215 2235 Size: 32 21 32 424 32 3407 32 2243 Fragments arranged by size: 3407 2243 424 32 32 32 32 21 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6223 XbaI T'CTAG_A Cuts at: 2722 3559 2722 Size: 837 5386 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2146 2146 Size: 6223 XhoI C'TCGA_G Cuts at: 1943 2403 1943 Size: 460 5763 XmaI C'CCGG_G Cuts at: 1870 3548 1870 Size: 1678 4545 XmnI GAAnn'nnTTC Cuts at: 2387 4352 2387 Size: 1965 4258 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4031 276 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfoI SrfI SwaI