Restriction map for
pFN21AE0504
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsiHKAI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp509I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 411 GGCGTC G[GA]^CG[CT]C
AgeI 257 ACCGGT A^CCGGT
AlwI 479 GGATC GGATC(4/5)
AlwNI 151 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 376 CCTGAGG CC^T[ACGT]AGG
AspI 276 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 353 CCCGG CC^[GC]GG
BsaHI 411 GGCGTC G[GA]^CG[CT]C
BsaI 458 GAGACC GGTCTC(1/5)
BsaWI 257 ACCGGT [AT]^CCGG[AT]
BsgI 286 GTGCAG GTGCAG(16/14)
BsmBI 607 CGTCTC CGTCTC(1/5)
BstYI 479 GGATCT [GA]^GATC[CT]
Bsu36I 376 CCTGAGG CC^T[ACGT]AGG
Cac8I 213 GCTGGC GC[ACGT]^[ACGT]GC
DpnII 480 GATC ^GATC
DsaI 419 CCACGG C^C[GA][CT]GG
EaeI 43 CGGCCG [CT]^GGCC[GA]
EagI 43 CGGCCG C^GGCCG
EclXI 43 CGGCCG C^GGCCG
Eco52I 43 CGGCCG C^GGCCG
Eco81I 376 CCTGAGG CC^T[ACGT]AGG
EcoT14I 571 CCAAGG C^C[AT][AT]GG
HgaI 412 GCGTC GACGC(5/10)
Hin1I 411 GGCGTC G[GA]^CG[CT]C
HindIII 175 AAGCTT A^AGCTT
MboI 480 GATC ^GATC
MflI 479 GGATCT [GA]^GATC[CT]
MseI 146 TTAA T^TAA
NciI 353 CCCGG CC^[GC]GG
NdeII 480 GATC ^GATC
NlaIII 505 CATG CATG^
NspI 504 ACATGC [GA]CATG^[CT]
PflFI 276 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 257 ACCGGT A^CCGGT
Sau3AI 480 GATC ^GATC
StyI 571 CCAAGG C^C[AT][AT]GG
Tru9I 146 TTAA T^TAA
TspRI 330 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 276 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 326 CCATCAGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 479 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 87 GTAC GT^AC
584 GTAC
AvaI 345 CTCGGG C^[CT]CG[GA]G
361 CTCGGG
AvaII 255 GGACC G^G[AT]CC
339 GGACC
BpmI 235 CTGGAG CTGGAG(16/14)
389 CTGGAG
BsiEI 36 CGACCG CG[GA][CT]^CG
43 CGGCCG
BsoBI 345 CTCGGG C^[CT]CG[GA]G
361 CTCGGG
BsrFI 38 ACCGGC [GA]^CCGG[CT]
257 ACCGGT
BstF5I 416 CATCC GGATG(2/0)
538 CATCC
CfoI 189 GCGC GCG^C
600 GCGC
Cfr10I 38 ACCGGC [GA]^CCGG[CT]
257 ACCGGT
EarI 75 GAAGAG CTCTTC(1/4)
226 GAAGAG
EcoNI 376 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
440 CCTTGCCCAGG
FauI 81 GCGGG CCCGC(4/6)
632 GCGGG
FokI 416 CATCC GGATG(9/13)
538 CATCC
HaeII 188 AGCGCC [GA]GCGC^[CT]
599 AGCGCC
HhaI 189 GCGC GCG^C
600 GCGC
HinP1I 189 GCGC G^CGC
600 GCGC
HinfI 280 GAGTC G^A[ACGT]TC
556 GACTC
Ksp632I 75 GAAGAG CTCTTC(1/4)
226 GAAGAG
PleI 556 GACTC GAGTC(4/5)
280 GAGTC
RsaI 87 GTAC GT^AC
584 GTAC
SfaNI 210 GATGC GCATC(5/9)
496 GCATC
TaqI 65 TCGA T^CGA
116 TCGA
TthHB8I 65 TCGA T^CGA
116 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI ApaI BanII BbvI
BmyI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
HaeIII HapII HpaII HphI ItaI
MaeIII MboII MnlI MspA1I MspI
MvaI MwoI NlaIV Sau96I ScrFI
TseI Tsp45I