(Circular) (Six-base) MAPSORT of: pFN21AE0504.seq Check: 9476 from: 1 to: 5344 pFN21AE0504 5344 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3154 278 Size: 53 83 186 936 1618 2468 Fragments arranged by size: 2468 1618 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2687 1937 Size: 750 4594 Acc65I G'GTAC_C Cuts at: 2665 2665 Size: 5344 AclI AA'CG_TT Cuts at: 1563 3471 3844 1563 Size: 1908 373 3063 Fragments arranged by size: 3063 1908 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3407 4791 5059 1632 Size: 1775 1384 268 1917 Fragments arranged by size: 1917 1775 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5147 AflIII A'CryG_T Cuts at: 1203 4243 5220 1203 Size: 3040 977 1327 Fragments arranged by size: 3040 1327 977 AgeI A'CCGG_T Cuts at: 2262 4175 2262 Size: 1913 3431 AhdI GACnn_n'nnGTC Cuts at: 4073 4073 Size: 5344 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5344 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5344 AlwNI CAG_nnn'CTG Cuts at: 2161 3087 4659 2161 Size: 926 1572 2846 Fragments arranged by size: 2846 1572 926 ApaI G_GGCC'C Cuts at: 2359 2557 2584 2359 Size: 198 27 5119 Fragments arranged by size: 5119 198 27 ApaLI G'TGCA_C Cuts at: 3401 4557 3401 Size: 1156 4188 ApoI r'AATT_y Cuts at: 1493 2653 2879 3031 5140 1493 Size: 1160 226 152 2109 1697 Fragments arranged by size: 2109 1697 1160 226 152 AseI AT'TA_AT Cuts at: 160 1032 3898 160 Size: 872 2866 1606 Fragments arranged by size: 2866 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5344 AvaI C'yCGr_G Cuts at: 1870 1943 2350 2366 2669 1870 Size: 73 407 16 303 4545 Fragments arranged by size: 4545 407 303 73 16 BamHI G'GATC_C Cuts at: 2674 3041 2674 Size: 367 4977 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2665 4120 618 Size: 325 217 627 878 1455 1842 Fragments arranged by size: 1842 1455 878 627 325 217 BanII G_rGCy'C Cuts at: 1391 2359 2557 2584 2663 1391 Size: 968 198 27 79 4072 Fragments arranged by size: 4072 968 198 79 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4373 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3535 3569 1903 Size: 34 1598 34 3678 Fragments arranged by size: 3678 1598 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3236 4446 3236 Size: 1210 4134 BclI T'GATC_A Cuts at: 1548 1548 Size: 5344 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3955 136 Size: 107 122 71 631 605 2283 1525 Fragments arranged by size: 2283 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5339 5339 Size: 5344 BlpI GC'TnA_GC Cuts at: 2761 2761 Size: 5344 Bme1580I G_kGCm'C Cuts at: 1790 2359 2557 2584 3405 4561 1790 Size: 569 198 27 821 1156 2573 Fragments arranged by size: 2573 1156 821 569 198 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5344 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3075 4033 442 Size: 416 53 83 399 24 1658 958 1753 Fragments arranged by size: 1753 1658 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5344 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2415 4004 1239 Size: 1022 154 1589 2579 Fragments arranged by size: 2579 1589 1022 154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3469 4334 4632 3469 Size: 865 298 4181 Fragments arranged by size: 4181 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2457 4007 882 Size: 617 958 1550 2219 Fragments arranged by size: 2219 1550 958 617 BsaAI yAC'GTr Cuts at: 493 1204 3135 493 Size: 711 1931 2702 Fragments arranged by size: 2702 1931 711 BsaBI GATnn'nnATC Cuts at: 5338 5338 Size: 5344 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2417 3151 3533 4168 Size: 53 83 186 936 884 734 382 635 Cuts at: 4168 275 Size: 1451 Fragments arranged by size: 1451 936 884 734 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2262 3777 4175 4449 4596 5007 2262 Size: 1515 398 274 147 411 2599 Fragments arranged by size: 2599 1515 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2402 2432 1178 Size: 30 165 30 999 30 4090 Fragments arranged by size: 4090 999 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2159 2183 2204 2258 2423 2426 2479 Size: 904 69 24 21 54 165 3 53 Cuts at: 2479 1186 Size: 4051 Fragments arranged by size: 4051 904 165 69 54 53 24 21 3 BseYI C'CCAG_C Cuts at: 2601 4547 5043 2601 Size: 1946 496 2902 Fragments arranged by size: 2902 1946 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2312 1163 Size: 573 576 4195 Fragments arranged by size: 4195 576 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2044 2051 2794 3555 4583 1540 Size: 275 186 43 7 743 761 1028 2301 Fragments arranged by size: 2301 1028 761 743 275 186 43 7 BsiHKAI G_wGCw'C Cuts at: 3405 3490 4561 3405 Size: 85 1071 4188 Fragments arranged by size: 4188 1071 85 BsmI GAATG_Cn' Cuts at: 2855 2948 2855 Size: 93 5251 BsmBI CGTCTCn'nnnn_ Cuts at: 2618 2618 Size: 5344 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2359 2557 2584 2663 3405 3490 4561 Size: 399 569 198 27 79 742 85 1071 Cuts at: 4561 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 569 399 198 85 79 27 BspEI T'CCGG_A Cuts at: 5007 5007 Size: 5344 BspHI T'CATG_A Cuts at: 3233 4963 3233 Size: 1730 3614 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2699 844 Size: 1855 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3231 1394 Size: 429 1408 3507 Fragments arranged by size: 3507 1408 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3839 4013 65 Size: 1183 2591 174 1396 Fragments arranged by size: 2591 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2043 2262 3988 4175 1700 Size: 110 233 219 1726 187 2869 Fragments arranged by size: 2869 1726 233 219 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5344 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5344 BssSI C'ACGA_G Cuts at: 3404 4416 3404 Size: 1012 4332 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5344 BstBI TT'CG_AA Cuts at: 4148 4148 Size: 5344 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5344 BstYI r'GATC_y Cuts at: 1972 2484 2674 3041 3426 3443 4895 4981 4993 Size: 512 190 367 385 17 1452 86 12 Cuts at: 4993 5339 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 512 385 367 346 190 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2382 2382 Size: 5344 BtgI C'CryG_G Cuts at: 513 1065 2004 2424 513 Size: 552 939 420 3433 Fragments arranged by size: 3433 939 552 420 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4605 Fragments arranged by size: 4605 683 56 BtsI GCAGTG_nn' Cuts at: 2860 3654 3674 2860 Size: 794 20 4530 Fragments arranged by size: 4530 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5344 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5309 DraI TTT'AAA Cuts at: 2648 2648 Size: 5344 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4351 809 Size: 507 3035 1802 Fragments arranged by size: 3035 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2048 2791 3680 Size: 54 1425 50 275 18 218 743 889 Cuts at: 3680 5309 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 743 275 218 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2048 2791 1537 Size: 275 236 743 4090 Fragments arranged by size: 4090 743 275 236 EarI CTCTTCn'nnn_ Cuts at: 1353 2075 2226 3274 1353 Size: 722 151 1048 3423 Fragments arranged by size: 3423 1048 722 151 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3931 4305 4451 387 Size: 1064 138 2342 374 146 1280 Fragments arranged by size: 2342 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2415 3407 4004 4791 5059 1239 Size: 393 629 154 992 597 787 268 1524 Fragments arranged by size: 1524 992 787 629 597 393 268 154 EcoNI CCTnn'n_nnAGG Cuts at: 2385 2449 2385 Size: 64 5280 EcoO109I rG'GnC_Cy Cuts at: 2355 2580 2581 2355 Size: 225 1 5118 Fragments arranged by size: 5118 225 1 EcoRI G'AATT_C Cuts at: 2653 3031 2653 Size: 378 4966 FspI TGC'GCA Cuts at: 3850 3850 Size: 5344 HaeII r_GCGC'y Cuts at: 1678 2197 2608 4491 1678 Size: 519 411 1883 2531 Fragments arranged by size: 2531 1883 519 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2405 2434 2437 2527 Size: 32 135 32 830 3 29 3 90 Cuts at: 2527 2559 3985 4017 4059 4091 1373 Size: 32 1426 32 42 32 2626 Fragments arranged by size: 2626 1426 830 135 90 42 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2688 2934 669 Size: 988 281 750 246 3079 Fragments arranged by size: 3079 988 750 281 246 HindIII A'AGCT_T Cuts at: 748 2180 748 Size: 1432 3912 HpaI GTT'AAC Cuts at: 2934 2934 Size: 5344 KpnI G_GTAC'C Cuts at: 2669 2669 Size: 5344 MfeI C'AATT_G Cuts at: 2943 2943 Size: 5344 MluI A'CGCG_T Cuts at: 5220 5220 Size: 5344 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4458 4642 1305 Size: 3153 184 2007 Fragments arranged by size: 3153 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3522 Fragments arranged by size: 3522 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3302 3661 3820 518 Size: 648 2136 359 159 2042 Fragments arranged by size: 2136 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2482 2637 2760 3437 4585 4830 1606 Size: 552 324 155 123 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 552 324 245 155 123 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3853 Fragments arranged by size: 3853 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5344 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5344 NotI GC'GGCC_GC Cuts at: 2791 2791 Size: 5344 NspI r_CATG'y Cuts at: 1132 1229 2513 2702 4247 1132 Size: 97 1284 189 1545 2229 Fragments arranged by size: 2229 1545 1284 189 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5344 PciI A'CATG_T Cuts at: 4243 4243 Size: 5344 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5344 PmeI GTTT'AAAC Cuts at: 2648 2648 Size: 5344 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3413 3445 4926 4958 3413 Size: 32 1481 32 3799 Fragments arranged by size: 3799 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5344 PsiI TTA'TAA Cuts at: 2914 2914 Size: 5344 PspOMI G'GGCC_C Cuts at: 2355 2553 2580 2355 Size: 198 27 5119 Fragments arranged by size: 5119 198 27 PstI C_TGCA'G Cuts at: 830 2696 830 Size: 1866 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5344 SalI G'TCGA_C Cuts at: 1936 2686 1936 Size: 750 4594 SbfI CC_TGCA'GG Cuts at: 2696 2696 Size: 5344 ScaI AGT'ACT Cuts at: 3592 5304 3592 Size: 1712 3632 SfcI C'TryA_G Cuts at: 826 1045 2692 3827 4508 4699 826 Size: 219 1647 1135 681 191 1471 Fragments arranged by size: 1647 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5344 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5344 SmaI CCC'GGG Cuts at: 1872 2671 1872 Size: 799 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3448 4349 4611 820 Size: 197 926 1505 901 262 1553 Fragments arranged by size: 1553 1505 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3135 493 Size: 2642 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5344 SphI G_CATG'C Cuts at: 1132 2702 1132 Size: 1570 3774 SspI AAT'ATT Cuts at: 5 52 3268 5 Size: 47 3216 2081 Fragments arranged by size: 3216 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5344 StyI C'CwwG_G Cuts at: 513 1065 2004 2576 2783 513 Size: 552 939 572 207 3074 Fragments arranged by size: 3074 939 572 552 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3371 3388 3541 3726 4171 325 Size: 1255 1791 17 153 185 445 1498 Fragments arranged by size: 1791 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3590 5302 96 Size: 274 80 33 51 1443 1613 1712 138 Fragments arranged by size: 1712 1613 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5304 5336 5304 Size: 32 5312 Tth111I GACn'n_nGTC Cuts at: 1316 2284 1316 Size: 968 4376 XbaI T'CTAG_A Cuts at: 2680 2680 Size: 5344 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 2338 Size: 5344 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5344 XmaI C'CCGG_G Cuts at: 1870 2669 1870 Size: 799 4545 XmnI GAAnn'nnTTC Cuts at: 3473 3473 Size: 5344 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3152 276 Size: 53 83 186 936 1618 2468 Fragments arranged by size: 2468 1618 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI