Restriction map for
pFN21AE0504
(Linear) (Six-base) MAPSORT of: KIEE0504.seq Check: 1267 from: 1 to: 639
KIEE0504.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AgeI A'CCGG_T
Cuts at: 0 257 639
Size: 257 382
AlwNI CAG_nnn'CTG
Cuts at: 0 156 639
Size: 156 483
ApaI G_GGCC'C
Cuts at: 0 354 552 579 639
Size: 354 198 27 60
Fragments arranged by size:
354 198 60 27
AvaI C'yCGr_G
Cuts at: 0 345 361 639
Size: 345 16 278
Fragments arranged by size:
345 278 16
BanII G_rGCy'C
Cuts at: 0 354 552 579 639
Size: 354 198 27 60
Fragments arranged by size:
354 198 60 27
Bme1580I G_kGCm'C
Cuts at: 0 354 552 579 639
Size: 354 198 27 60
Fragments arranged by size:
354 198 60 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 410 639
Size: 256 154 229
Fragments arranged by size:
256 229 154
BsaI GGTCTCn'nnnn_
Cuts at: 0 452 639
Size: 452 187
BsaHI Gr'CG_yC
Cuts at: 0 412 639
Size: 412 227
BsaWI w'CCGG_w
Cuts at: 0 257 639
Size: 257 382
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 397 427 639
Size: 397 30 212
Fragments arranged by size:
397 212 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 154 178 199 253 418 421 474
Size: 85 69 24 21 54 165 3 53
Cuts at: 474 639
Size: 165
Fragments arranged by size:
165 165 85 69 54 53 24 21
3
BseYI C'CCAG_C
Cuts at: 0 596 639
Size: 596 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 639
Size: 307 332
BsiEI CG_ry'CG
Cuts at: 0 39 46 639
Size: 39 7 593
Fragments arranged by size:
593 39 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 613 639
Size: 613 26
Bsp1286I G_dGCh'C
Cuts at: 0 354 552 579 639
Size: 354 198 27 60
Fragments arranged by size:
354 198 60 27
BsrFI r'CCGG_y
Cuts at: 0 38 257 639
Size: 38 219 382
Fragments arranged by size:
382 219 38
BstYI r'GATC_y
Cuts at: 0 479 639
Size: 479 160
Bsu36I CC'TnA_GG
Cuts at: 0 377 639
Size: 377 262
BtgI C'CryG_G
Cuts at: 0 419 639
Size: 419 220
EaeI y'GGCC_r
Cuts at: 0 43 639
Size: 43 596
EagI C'GGCC_G
Cuts at: 0 43 639
Size: 43 596
EarI CTCTTCn'nnn_
Cuts at: 0 70 221 639
Size: 70 151 418
Fragments arranged by size:
418 151 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 410 639
Size: 256 154 229
Fragments arranged by size:
256 229 154
EcoNI CCTnn'n_nnAGG
Cuts at: 0 380 444 639
Size: 380 64 195
Fragments arranged by size:
380 195 64
EcoO109I rG'GnC_Cy
Cuts at: 0 350 575 576 639
Size: 350 225 1 63
Fragments arranged by size:
350 225 63 1
HaeII r_GCGC'y
Cuts at: 0 192 603 639
Size: 192 411 36
Fragments arranged by size:
411 192 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 400 429 432 522 554 639
Size: 397 3 29 3 90 32 85
Fragments arranged by size:
397 90 85 32 29 3 3
HindIII A'AGCT_T
Cuts at: 0 175 639
Size: 175 464
MspA1I CmG'CkG
Cuts at: 0 153 477 632 639
Size: 153 324 155 7
Fragments arranged by size:
324 155 153 7
NspI r_CATG'y
Cuts at: 0 508 639
Size: 508 131
PspOMI G'GGCC_C
Cuts at: 0 350 548 575 639
Size: 350 198 27 64
Fragments arranged by size:
350 198 64 27
StyI C'CwwG_G
Cuts at: 0 571 639
Size: 571 68
Tth111I GACn'n_nGTC
Cuts at: 0 279 639
Size: 279 360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 639
Size: 333 306
Enzymes that do cut:
AgeI AlwNI ApaI AvaI BanII Bme1580I BpmI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BsrFI BstYI Bsu36I BtgI EaeI EagI EarI
Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MspA1I NspI
PspOMI StyI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI XbaI XhoI XmaI XmnI ZraI