Restriction map for
pFN21AB7318
##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PmeI
PshAI PshBI PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AclI 735 AACGTT AA^CGTT
AcyI 1999 GACGCC G[GA]^CG[CT]C
AflIII 1730 ACGTGT A^C[GA][CT]GT
Aor51HI 1529 AGCGCT AGC^GCT
BcnI 1241 CCGGG CC^[GC]GG
BglII 2597 AGATCT A^GATCT
BsaHI 1999 GACGCC G[GA]^CG[CT]C
BsaI 1947 GGTCTC GGTCTC(1/5)
BsmBI 1172 GAGACG CGTCTC(1/5)
BsrBI 2326 GAGCGG CCGCTC(-3/-3)
BsrDI 195 GCAATG GCAATG(2/0)
BssSI 1861 CTCGTG CACGAG(-5/-1)
DraIII 53 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1785 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1529 AGCGCT AGC^GCT
EcoNI 2235 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 1999 GACGCC G[GA]^CG[CT]C
NciI 1241 CCGGG CC^[GC]GG
NcoI 1796 CCATGG C^CATGG
Psp1406I 735 AACGTT AA^CGTT
SapI 3144 GAAGAGC GCTCTTC(1/4)
ScaI 1676 AGTACT AGT^ACT
SexAI 3326 ACCTGGT A^CC[AT]GGT
StuI 1440 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 1273 CGCG CG^CG
3096 CGCG
AgeI 761 ACCGGT A^CCGGT
3524 ACCGGT
BalI 961 TGGCCA TGG^CCA
2444 TGGCCA
BbrPI 56 CACGTG CAC^GTG
655 CACGTG
BfaI 214 CTAG C^TAG
826 CTAG
BsmI 306 GAATGC GAATGC(1/-1)
3504 GCATTC
BspHI 1316 TCATGA T^CATGA
3035 TCATGA
BstUI 1273 CGCG CG^CG
3096 CGCG
EarI 2494 GAAGAG CTCTTC(1/4)
3144 GAAGAG
HaeII 1493 GGCGCT [GA]GCGC^[CT]
1529 AGCGCT
HindIII 1933 AAGCTT A^AGCTT
3019 AAGCTT
Ksp632I 2494 GAAGAG CTCTTC(1/4)
3144 GAAGAG
MaeI 214 CTAG C^TAG
826 CTAG
MluNI 961 TGGCCA TGG^CCA
2444 TGGCCA
MscI 961 TGGCCA TGG^CCA
2444 TGGCCA
MvnI 1273 CGCG CG^CG
3096 CGCG
PaeR7I 1478 CTCGAG C^TCGAG
3126 CTCGAG
PinAI 761 ACCGGT A^CCGGT
3524 ACCGGT
PmaCI 56 CACGTG CAC^GTG
655 CACGTG
PmlI 56 CACGTG CAC^GTG
655 CACGTG
RcaI 1316 TCATGA T^CATGA
3035 TCATGA
SacI 132 GAGCTC GAGCT^C
1621 GAGCTC
XcmI 2819 CCAACAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3317 CCAGTCTAAACCTGG
XhoI 1478 CTCGAG C^TCGAG
3126 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BmyI BpmI BpuAI BsaAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI