(Circular) (Six-base) MAPSORT of: pFN21AB7318.seq Check: 608 from: 1 to: 8248 pFN21AB7318 8248 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4302 4382 4302 Size: 80 8168 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6058 278 Size: 53 83 186 936 4522 2468 Fragments arranged by size: 4522 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5591 1937 Size: 3654 4594 Acc65I G'GTAC_C Cuts at: 5569 5569 Size: 8248 AclI AA'CG_TT Cuts at: 1563 2741 5547 6375 6748 1563 Size: 1178 2806 828 373 3063 Fragments arranged by size: 3063 2806 1178 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2253 2487 3551 4514 4619 5168 5234 5332 Size: 621 234 1064 963 105 549 66 98 Cuts at: 5332 6311 7695 7963 1632 Size: 979 1384 268 1917 Fragments arranged by size: 1917 1384 1064 979 963 621 549 268 234 105 98 66 AfeI AGC'GCT Cuts at: 3536 3536 Size: 8248 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8051 AflIII A'CryG_T Cuts at: 1203 3735 7147 8124 1203 Size: 2532 3412 977 1327 Fragments arranged by size: 3412 2532 1327 977 AgeI A'CCGG_T Cuts at: 2766 5529 7079 2766 Size: 2763 1550 3935 Fragments arranged by size: 3935 2763 1550 AhdI GACnn_n'nnGTC Cuts at: 6977 6977 Size: 8248 AleI CACnn'nnGTG Cuts at: 1166 4813 1166 Size: 3647 4601 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3993 1184 Size: 2809 5439 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3243 3275 3243 Size: 32 8216 AlwNI CAG_nnn'CTG Cuts at: 3672 4099 4390 4729 4774 4900 5071 5991 7563 Size: 427 291 339 45 126 171 920 1572 Cuts at: 7563 3672 Size: 4357 Fragments arranged by size: 4357 1572 920 427 339 291 171 126 45 ApaLI G'TGCA_C Cuts at: 6305 7461 6305 Size: 1156 7092 ApoI r'AATT_y Cuts at: 1493 2231 2514 2571 2672 4152 5557 5783 5935 Size: 738 283 57 101 1480 1405 226 152 Cuts at: 5935 8044 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1480 1405 738 283 226 152 101 57 AseI AT'TA_AT Cuts at: 160 1032 6802 160 Size: 872 5770 1606 Fragments arranged by size: 5770 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8248 AvaI C'yCGr_G Cuts at: 1870 1943 3483 3614 5131 5243 5484 5573 1870 Size: 73 1540 131 1517 112 241 89 4545 Fragments arranged by size: 4545 1540 1517 241 131 112 89 73 BamHI G'GATC_C Cuts at: 5578 5945 5578 Size: 367 7881 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2597 3355 4280 4484 5569 Size: 325 217 627 810 758 925 204 1085 Cuts at: 5569 7024 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1085 925 810 758 627 325 217 204 BanII G_rGCy'C Cuts at: 1391 2141 3053 3371 3630 5567 1391 Size: 750 912 318 259 1937 4072 Fragments arranged by size: 4072 1937 912 750 318 259 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2887 3305 3794 3882 4349 5016 928 Size: 971 988 418 489 88 467 667 4160 Fragments arranged by size: 4160 988 971 667 489 467 418 88 BbvCI CC'TCA_GC Cuts at: 2679 3131 4197 4361 4780 2679 Size: 452 1066 164 419 6147 Fragments arranged by size: 6147 1066 452 419 164 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6439 6473 1903 Size: 34 4502 34 3678 Fragments arranged by size: 4502 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6140 7350 6140 Size: 1210 7038 BclI T'GATC_A Cuts at: 1548 1548 Size: 8248 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6859 136 Size: 107 122 71 631 605 5187 1525 Fragments arranged by size: 5187 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4602 8243 4602 Size: 3641 4607 BlpI GC'TnA_GC Cuts at: 5665 5665 Size: 8248 Bme1580I G_kGCm'C Cuts at: 1790 3188 3360 6309 7465 1790 Size: 1398 172 2949 1156 2573 Fragments arranged by size: 2949 2573 1398 1156 172 BmgBI CAC'GTC Cuts at: 1318 3181 3403 5397 1318 Size: 1863 222 1994 4169 Fragments arranged by size: 4169 1994 1863 222 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3097 3958 4705 Size: 416 53 83 399 24 1680 861 747 Cuts at: 4705 5979 6937 442 Size: 1274 958 1753 Fragments arranged by size: 1753 1680 1274 958 861 747 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8248 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2971 5305 2971 Size: 2334 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2941 3533 3692 3914 4112 4456 4757 5048 Size: 1702 592 159 222 198 344 301 291 Cuts at: 5048 5093 5480 6908 1239 Size: 45 387 1428 2579 Fragments arranged by size: 2579 1702 1428 592 387 344 301 291 222 198 159 45 Bpu10I CC'TnA_GC Cuts at: 2135 2679 3131 4197 4361 4780 4985 4991 2135 Size: 544 452 1066 164 419 205 6 5392 Fragments arranged by size: 5392 1066 544 452 419 205 164 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3182 3293 4014 4720 5462 6373 7238 7536 3182 Size: 111 721 706 742 911 865 298 3894 Fragments arranged by size: 3894 911 865 742 721 706 298 111 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3958 6911 882 Size: 617 2459 2953 2219 Fragments arranged by size: 2953 2459 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2063 2662 3736 6039 493 Size: 711 859 599 1074 2303 2702 Fragments arranged by size: 2702 2303 1074 859 711 599 BsaBI GATnn'nnATC Cuts at: 8242 8242 Size: 8248 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4005 6055 6437 7072 Size: 53 83 186 936 2472 2050 382 635 Cuts at: 7072 275 Size: 1451 Fragments arranged by size: 2472 2050 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2766 2983 4797 5529 6681 7079 7353 7500 7911 Size: 217 1814 732 1152 398 274 147 411 Cuts at: 7911 2766 Size: 3103 Fragments arranged by size: 3103 1814 1152 732 411 398 274 217 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2984 3014 3548 3578 1178 Size: 30 165 30 1581 30 534 30 5848 Fragments arranged by size: 5848 1581 534 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2327 2696 2699 3692 4262 5149 1186 Size: 1141 369 3 993 570 887 4285 Fragments arranged by size: 4285 1141 993 887 570 369 3 BseYI C'CCAG_C Cuts at: 2853 3904 4628 7451 7947 2853 Size: 1051 724 2823 496 3154 Fragments arranged by size: 3154 2823 1051 724 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2783 3434 3929 4445 5357 5489 1163 Size: 573 1047 651 495 516 912 132 3922 Fragments arranged by size: 3922 1047 912 651 573 516 495 132 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5698 6459 7487 1540 Size: 275 186 3697 761 1028 2301 Fragments arranged by size: 3697 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2068 2141 3630 3637 6309 6394 7465 2068 Size: 73 1489 7 2672 85 1071 2851 Fragments arranged by size: 2851 2672 1489 1071 85 73 7 BsmI GAATG_Cn' Cuts at: 2317 5509 5759 5852 2317 Size: 3192 250 93 4713 Fragments arranged by size: 4713 3192 250 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3171 3171 Size: 8248 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2141 3053 3188 3360 3371 3630 Size: 399 278 73 912 135 172 11 259 Cuts at: 3630 3637 5567 6309 6394 7465 1391 Size: 7 1930 742 85 1071 2174 Fragments arranged by size: 2174 1930 1071 912 742 399 278 259 172 135 85 73 11 7 BspEI T'CCGG_A Cuts at: 7911 7911 Size: 8248 BspHI T'CATG_A Cuts at: 3321 5040 6137 7867 3321 Size: 1719 1097 1730 3702 Fragments arranged by size: 3702 1730 1719 1097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2415 4302 4382 5603 844 Size: 1571 1887 80 1221 3489 Fragments arranged by size: 3489 1887 1571 1221 80 BsrBI CCG'CTC Cuts at: 1394 1823 4333 6135 1394 Size: 429 2510 1802 3507 Fragments arranged by size: 3507 2510 1802 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2207 6743 6917 65 Size: 1183 959 4536 174 1396 Fragments arranged by size: 4536 1396 1183 959 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2766 5310 5529 6892 7079 1700 Size: 110 956 2544 219 1363 187 2869 Fragments arranged by size: 2869 2544 1363 956 219 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8248 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8248 BssSI C'ACGA_G Cuts at: 3866 6308 7320 3866 Size: 2442 1012 4794 Fragments arranged by size: 4794 2442 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8248 BstBI TT'CG_AA Cuts at: 7052 7052 Size: 8248 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8248 BstYI r'GATC_y Cuts at: 1972 2461 4173 4466 4602 5158 5578 5945 6330 Size: 489 1712 293 136 556 420 367 385 Cuts at: 6330 6347 7799 7885 7897 8243 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1712 1452 556 489 420 385 367 346 293 136 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3363 3801 4758 513 Size: 552 2298 438 957 4003 Fragments arranged by size: 4003 2298 957 552 438 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2346 487 Size: 683 56 1120 6389 Fragments arranged by size: 6389 1120 683 56 BtsI GCAGTG_nn' Cuts at: 2712 2944 4259 5764 6558 6578 2712 Size: 232 1315 1505 794 20 4382 Fragments arranged by size: 4382 1505 1315 794 232 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8248 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3821 3856 4058 4093 529 Size: 35 3257 35 202 35 4684 Fragments arranged by size: 4684 3257 202 35 35 35 DraI TTT'AAA Cuts at: 5552 5552 Size: 8248 DraIII CAC_nnn'GTG Cuts at: 2063 2063 Size: 8248 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3796 7255 809 Size: 507 2480 3459 1802 Fragments arranged by size: 3459 2480 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2726 2966 4449 Size: 54 1425 50 275 18 896 240 1483 Cuts at: 4449 5695 6584 8213 8 Size: 1246 889 1629 43 Fragments arranged by size: 1629 1483 1425 1246 896 889 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5695 1537 Size: 275 3883 4090 Fragments arranged by size: 4090 3883 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4494 5144 6178 1353 Size: 3141 650 1034 3423 Fragments arranged by size: 3423 3141 1034 650 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6835 7209 7355 387 Size: 1064 138 5246 374 146 1280 Fragments arranged by size: 5246 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2139 3628 2139 Size: 1489 6759 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2253 2487 2941 3533 3551 3692 3914 Size: 393 621 234 454 592 18 141 222 Cuts at: 3914 4112 4456 4514 4619 4757 5048 5093 5168 Size: 198 344 58 105 138 291 45 75 Cuts at: 5168 5234 5332 5480 6311 6908 7695 7963 1239 Size: 66 98 148 831 597 787 268 1524 Fragments arranged by size: 1524 831 787 621 597 592 454 393 344 291 268 234 222 198 148 141 138 105 98 75 66 58 45 18 EcoNI CCTnn'n_nnAGG Cuts at: 4244 4244 Size: 8248 EcoO109I rG'GnC_Cy Cuts at: 2087 3139 3745 3898 5068 2087 Size: 1052 606 153 1170 5267 Fragments arranged by size: 5267 1170 1052 606 153 EcoRI G'AATT_C Cuts at: 5557 5935 5557 Size: 378 7870 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3871 4097 3871 Size: 226 8022 FspI TGC'GCA Cuts at: 6754 6754 Size: 8248 HaeII r_GCGC'y Cuts at: 1678 3502 3538 7395 1678 Size: 1824 36 3857 2531 Fragments arranged by size: 3857 2531 1824 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2984 3016 3035 3067 3197 Size: 32 135 32 1412 32 19 32 130 Cuts at: 3197 3229 3477 3509 4253 4285 4894 4926 5102 Size: 32 248 32 744 32 609 32 176 Cuts at: 5102 5134 5143 5175 6889 6921 6963 6995 1373 Size: 32 9 32 1714 32 42 32 2626 Fragments arranged by size: 2626 1714 1412 744 609 248 176 135 130 42 32 32 32 32 32 32 32 32 32 32 32 32 19 9 HincII GTy'rAC Cuts at: 669 1657 1938 5592 5838 669 Size: 988 281 3654 246 3079 Fragments arranged by size: 3654 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3938 5024 748 Size: 3190 1086 3972 Fragments arranged by size: 3972 3190 1086 HpaI GTT'AAC Cuts at: 5838 5838 Size: 8248 KpnI G_GTAC'C Cuts at: 5573 5573 Size: 8248 MfeI C'AATT_G Cuts at: 5847 5847 Size: 8248 MluI A'CGCG_T Cuts at: 8124 8124 Size: 8248 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4530 4848 7362 7546 1305 Size: 3225 318 2514 184 2007 Fragments arranged by size: 3225 2514 2007 318 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2968 4451 10 Size: 54 1425 343 1136 1483 3807 Fragments arranged by size: 3807 1483 1425 1136 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2634 3380 4813 6206 6565 6724 518 Size: 648 1468 746 1433 1393 359 159 2042 Fragments arranged by size: 2042 1468 1433 1393 746 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2549 2923 3394 4196 4303 5664 6341 7489 Size: 943 374 471 802 107 1361 677 1148 Cuts at: 7489 7734 1606 Size: 245 2120 Fragments arranged by size: 2120 1361 1148 943 802 677 471 374 245 107 NcoI C'CATG_G Cuts at: 513 1065 3801 513 Size: 552 2736 4960 Fragments arranged by size: 4960 2736 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8248 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8248 NotI GC'GGCC_GC Cuts at: 5695 5695 Size: 8248 NspI r_CATG'y Cuts at: 1132 1229 2286 4206 5259 5606 7151 1132 Size: 97 1057 1920 1053 347 1545 2229 Fragments arranged by size: 2229 1920 1545 1057 1053 347 97 PasI CC'CwG_GG Cuts at: 1348 3922 5176 1348 Size: 2574 1254 4420 Fragments arranged by size: 4420 2574 1254 PciI A'CATG_T Cuts at: 7147 7147 Size: 8248 PflMI CCAn_nnn'nTGG Cuts at: 1108 2964 4099 4774 5071 1108 Size: 1856 1135 675 297 4285 Fragments arranged by size: 4285 1856 1135 675 297 PfoI T'CCnGG_A Cuts at: 3708 5064 3708 Size: 1356 6892 PmeI GTTT'AAAC Cuts at: 5552 5552 Size: 8248 PmlI CAC'GTG Cuts at: 2063 2662 2063 Size: 599 7649 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6317 6349 7830 7862 6317 Size: 32 1481 32 6703 Fragments arranged by size: 6703 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2087 3139 3898 5068 2087 Size: 1052 759 1170 5267 Fragments arranged by size: 5267 1170 1052 759 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8248 PsiI TTA'TAA Cuts at: 5818 5818 Size: 8248 PspXI vC'TCGA_Gb Cuts at: 3483 3483 Size: 8248 PstI C_TGCA'G Cuts at: 830 2554 3393 3399 4725 4896 5600 830 Size: 1724 839 6 1326 171 704 3478 Fragments arranged by size: 3478 1724 1326 839 704 171 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8248 PvuII CAG'CTG Cuts at: 2549 3394 4303 2549 Size: 845 909 6494 Fragments arranged by size: 6494 909 845 SacI G_AGCT'C Cuts at: 2141 3630 2141 Size: 1489 6759 SalI G'TCGA_C Cuts at: 1936 5590 1936 Size: 3654 4594 SapI GCTCTTCn'nnn_ Cuts at: 5144 5144 Size: 8248 SbfI CC_TGCA'GG Cuts at: 5600 5600 Size: 8248 ScaI AGT'ACT Cuts at: 3683 6496 8208 3683 Size: 2813 1712 3723 Fragments arranged by size: 3723 2813 1712 SexAI A'CCwGG_T Cuts at: 5331 5331 Size: 8248 SfcI C'TryA_G Cuts at: 826 1045 2550 3389 3395 4721 4885 4892 5596 Size: 219 1505 839 6 1326 164 7 704 Cuts at: 5596 6731 7412 7603 826 Size: 1135 681 191 1471 Fragments arranged by size: 1505 1471 1326 1135 839 704 681 219 191 164 7 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8248 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8248 SmaI CCC'GGG Cuts at: 1872 5575 1872 Size: 3703 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3197 3272 3483 4029 4699 5131 Size: 197 926 1254 75 211 546 670 432 Cuts at: 5131 5477 6352 7253 7515 820 Size: 346 875 901 262 1553 Fragments arranged by size: 1553 1254 926 901 875 670 546 432 346 262 211 197 75 SnaBI TAC'GTA Cuts at: 493 6039 493 Size: 5546 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8248 SphI G_CATG'C Cuts at: 1132 5606 1132 Size: 4474 3774 SspI AAT'ATT Cuts at: 5 52 6172 5 Size: 47 6120 2081 Fragments arranged by size: 6120 2081 47 StuI AGG'CCT Cuts at: 1517 3447 1517 Size: 1930 6318 StyI C'CwwG_G Cuts at: 513 1065 2485 3801 5292 5687 513 Size: 552 1420 1316 1491 395 3074 Fragments arranged by size: 3074 1491 1420 1316 552 395 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4298 4302 4502 4551 5168 6275 6292 Size: 1255 2718 4 200 49 617 1107 17 Cuts at: 6292 6445 6630 7075 325 Size: 153 185 445 1498 Fragments arranged by size: 2718 1498 1255 1107 617 445 200 185 153 49 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3681 3729 6494 Size: 274 80 33 51 1443 1704 48 2765 Cuts at: 6494 8206 96 Size: 1712 138 Fragments arranged by size: 2765 1712 1704 1443 274 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3548 3580 3882 3914 4504 4536 4647 4679 4831 Size: 32 302 32 590 32 111 32 152 Cuts at: 4831 4863 8208 8240 3548 Size: 32 3345 32 3556 Fragments arranged by size: 3556 3345 590 302 152 111 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8248 XbaI T'CTAG_A Cuts at: 5584 5584 Size: 8248 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4831 5329 4831 Size: 498 7750 XhoI C'TCGA_G Cuts at: 1943 3483 5131 1943 Size: 1540 1648 5060 Fragments arranged by size: 5060 1648 1540 XmaI C'CCGG_G Cuts at: 1870 5573 1870 Size: 3703 4545 XmnI GAAnn'nnTTC Cuts at: 2232 3536 3851 5345 6377 2232 Size: 1304 315 1494 1032 4103 Fragments arranged by size: 4103 1494 1304 1032 315 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6056 276 Size: 53 83 186 936 4522 2468 Fragments arranged by size: 4522 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI