Restriction map for
pFN21AB7318
(Linear) (Six-base) MAPSORT of: KIBB7318.seq Check: 682 from: 1 to: 3543
KIBB7318.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2297 2377 3543
Size: 2297 80 1166
Fragments arranged by size:
2297 1166 80
AclI AA'CG_TT
Cuts at: 0 736 3543
Size: 736 2807
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 482 1546 2509 2614 3163 3229 3327
Size: 248 234 1064 963 105 549 66 98
Cuts at: 3327 3543
Size: 216
Fragments arranged by size:
1064 963 549 248 234 216 105 98
66
AfeI AGC'GCT
Cuts at: 0 1531 3543
Size: 1531 2012
AflIII A'CryG_T
Cuts at: 0 1730 3543
Size: 1730 1813
AgeI A'CCGG_T
Cuts at: 0 761 3524 3543
Size: 761 2763 19
Fragments arranged by size:
2763 761 19
AleI CACnn'nnGTG
Cuts at: 0 2808 3543
Size: 2808 735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1988 3543
Size: 1988 1555
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1238 1270 3543
Size: 1238 32 2273
Fragments arranged by size:
2273 1238 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1667 2094 2385 2724 2769 2895 3066 3543
Size: 1667 427 291 339 45 126 171 477
Fragments arranged by size:
1667 477 427 339 291 171 126 45
ApoI r'AATT_y
Cuts at: 0 226 509 566 667 2147 3543
Size: 226 283 57 101 1480 1396
Fragments arranged by size:
1480 1396 283 226 101 57
AvaI C'yCGr_G
Cuts at: 0 1478 1609 3126 3238 3479 3543
Size: 1478 131 1517 112 241 64
Fragments arranged by size:
1517 1478 241 131 112 64
BanI G'GyrC_C
Cuts at: 0 592 1350 2275 2479 3543
Size: 592 758 925 204 1064
Fragments arranged by size:
1064 925 758 592 204
BanII G_rGCy'C
Cuts at: 0 136 1048 1366 1625 3543
Size: 136 912 318 259 1918
Fragments arranged by size:
1918 912 318 259 136
BbsI GAAGACnn'nnnn_
Cuts at: 0 882 1300 1789 1877 2344 3011 3543
Size: 882 418 489 88 467 667 532
Fragments arranged by size:
882 667 532 489 467 418 88
BbvCI CC'TCA_GC
Cuts at: 0 674 1126 2192 2356 2775 3543
Size: 674 452 1066 164 419 768
Fragments arranged by size:
1066 768 674 452 419 164
BglII A'GATC_T
Cuts at: 0 2597 3543
Size: 2597 946
Bme1580I G_kGCm'C
Cuts at: 0 1183 1355 3543
Size: 1183 172 2188
Fragments arranged by size:
2188 1183 172
BmgBI CAC'GTC
Cuts at: 0 1176 1398 3392 3543
Size: 1176 222 1994 151
Fragments arranged by size:
1994 1176 222 151
BmrI ACTGGGnnnn_n'
Cuts at: 0 1092 1953 2700 3543
Size: 1092 861 747 843
Fragments arranged by size:
1092 861 843 747
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 966 3300 3543
Size: 966 2334 243
Fragments arranged by size:
2334 966 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 936 1528 1687 1909 2107 2451 2752 3043
Size: 936 592 159 222 198 344 301 291
Cuts at: 3043 3088 3475 3543
Size: 45 387 68
Fragments arranged by size:
936 592 387 344 301 291 222 198
159 68 45
Bpu10I CC'TnA_GC
Cuts at: 0 130 674 1126 2192 2356 2775 2980 2986
Size: 130 544 452 1066 164 419 205 6
Cuts at: 2986 3543
Size: 557
Fragments arranged by size:
1066 557 544 452 419 205 164 130
6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1288 2009 2715 3457 3543
Size: 1177 111 721 706 742 86
Fragments arranged by size:
1177 742 721 706 111 86
BsaI GGTCTCn'nnnn_
Cuts at: 0 1953 3543
Size: 1953 1590
BsaAI yAC'GTr
Cuts at: 0 58 657 1731 3543
Size: 58 599 1074 1812
Fragments arranged by size:
1812 1074 599 58
BsaHI Gr'CG_yC
Cuts at: 0 2000 3543
Size: 2000 1543
BsaWI w'CCGG_w
Cuts at: 0 761 978 2792 3524 3543
Size: 761 217 1814 732 19
Fragments arranged by size:
1814 761 732 217 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 979 1009 1543 1573 3543
Size: 979 30 534 30 1970
Fragments arranged by size:
1970 979 534 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 322 691 694 1687 2257 3144 3543
Size: 322 369 3 993 570 887 399
Fragments arranged by size:
993 887 570 399 369 322 3
BseYI C'CCAG_C
Cuts at: 0 848 1899 2623 3543
Size: 848 1051 724 920
Fragments arranged by size:
1051 920 848 724
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1429 1924 2440 3352 3484 3543
Size: 778 651 495 516 912 132 59
Fragments arranged by size:
912 778 651 516 495 132 59
BsiHKAI G_wGCw'C
Cuts at: 0 63 136 1625 1632 3543
Size: 63 73 1489 7 1911
Fragments arranged by size:
1911 1489 73 63 7
BsmI GAATG_Cn'
Cuts at: 0 312 3504 3543
Size: 312 3192 39
Fragments arranged by size:
3192 312 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1166 3543
Size: 1166 2377
Bsp1286I G_dGCh'C
Cuts at: 0 63 136 1048 1183 1355 1366 1625 1632
Size: 63 73 912 135 172 11 259 7
Cuts at: 1632 3543
Size: 1911
Fragments arranged by size:
1911 912 259 172 135 73 63 11
7
BspHI T'CATG_A
Cuts at: 0 1316 3035 3543
Size: 1316 1719 508
Fragments arranged by size:
1719 1316 508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 410 2297 2377 3543
Size: 410 1887 80 1166
Fragments arranged by size:
1887 1166 410 80
BsrBI CCG'CTC
Cuts at: 0 2328 3543
Size: 2328 1215
BsrDI GCAATG_nn'
Cuts at: 0 202 3543
Size: 202 3341
BsrFI r'CCGG_y
Cuts at: 0 761 3305 3524 3543
Size: 761 2544 219 19
Fragments arranged by size:
2544 761 219 19
BssSI C'ACGA_G
Cuts at: 0 1861 3543
Size: 1861 1682
BstYI r'GATC_y
Cuts at: 0 456 2168 2461 2597 3153 3543
Size: 456 1712 293 136 556 390
Fragments arranged by size:
1712 556 456 390 293 136
BtgI C'CryG_G
Cuts at: 0 1358 1796 2753 3543
Size: 1358 438 957 790
Fragments arranged by size:
1358 957 790 438
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 341 3543
Size: 341 3202
BtsI GCAGTG_nn'
Cuts at: 0 707 939 2254 3543
Size: 707 232 1315 1289
Fragments arranged by size:
1315 1289 707 232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1816 1851 2053 2088 3543
Size: 1816 35 202 35 1455
Fragments arranged by size:
1816 1455 202 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 58 3543
Size: 58 3485
DrdI GACnn_nn'nnGTC
Cuts at: 0 1791 3543
Size: 1791 1752
EaeI y'GGCC_r
Cuts at: 0 721 961 2444 3543
Size: 721 240 1483 1099
Fragments arranged by size:
1483 1099 721 240
EarI CTCTTCn'nnn_
Cuts at: 0 2489 3139 3543
Size: 2489 650 404
Fragments arranged by size:
2489 650 404
EcoICRI GAG'CTC
Cuts at: 0 134 1623 3543
Size: 134 1489 1920
Fragments arranged by size:
1920 1489 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 482 936 1528 1546 1687 1909 2107
Size: 248 234 454 592 18 141 222 198
Cuts at: 2107 2451 2509 2614 2752 3043 3088 3163 3229
Size: 344 58 105 138 291 45 75 66
Cuts at: 3229 3327 3475 3543
Size: 98 148 68
Fragments arranged by size:
592 454 344 291 248 234 222 198
148 141 138 105 98 75 68 66
58 45 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2239 3543
Size: 2239 1304
EcoO109I rG'GnC_Cy
Cuts at: 0 82 1134 1740 1893 3063 3543
Size: 82 1052 606 153 1170 480
Fragments arranged by size:
1170 1052 606 480 153 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1866 2092 3543
Size: 1866 226 1451
Fragments arranged by size:
1866 1451 226
HaeII r_GCGC'y
Cuts at: 0 1497 1533 3543
Size: 1497 36 2010
Fragments arranged by size:
2010 1497 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 979 1011 1030 1062 1192 1224 1472 1504
Size: 979 32 19 32 130 32 248 32
Cuts at: 1504 2248 2280 2889 2921 3097 3129 3138 3170
Size: 744 32 609 32 176 32 9 32
Cuts at: 3170 3543
Size: 373
Fragments arranged by size:
979 744 609 373 248 176 130 32
32 32 32 32 32 32 32 19
9
HindIII A'AGCT_T
Cuts at: 0 1933 3019 3543
Size: 1933 1086 524
Fragments arranged by size:
1933 1086 524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2525 2843 3543
Size: 2525 318 700
Fragments arranged by size:
2525 700 318
MscI TGG'CCA
Cuts at: 0 963 2446 3543
Size: 963 1483 1097
Fragments arranged by size:
1483 1097 963
MslI CAynn'nnrTG
Cuts at: 0 629 1375 2808 3543
Size: 629 746 1433 735
Fragments arranged by size:
1433 746 735 629
MspA1I CmG'CkG
Cuts at: 0 544 918 1389 2191 2298 3543
Size: 544 374 471 802 107 1245
Fragments arranged by size:
1245 802 544 471 374 107
NcoI C'CATG_G
Cuts at: 0 1796 3543
Size: 1796 1747
NspI r_CATG'y
Cuts at: 0 281 2201 3254 3543
Size: 281 1920 1053 289
Fragments arranged by size:
1920 1053 289 281
PasI CC'CwG_GG
Cuts at: 0 1917 3171 3543
Size: 1917 1254 372
Fragments arranged by size:
1917 1254 372
PflMI CCAn_nnn'nTGG
Cuts at: 0 959 2094 2769 3066 3543
Size: 959 1135 675 297 477
Fragments arranged by size:
1135 959 675 477 297
PfoI T'CCnGG_A
Cuts at: 0 1703 3059 3543
Size: 1703 1356 484
Fragments arranged by size:
1703 1356 484
PmlI CAC'GTG
Cuts at: 0 58 657 3543
Size: 58 599 2886
Fragments arranged by size:
2886 599 58
PpuMI rG'GwC_Cy
Cuts at: 0 82 1134 1893 3063 3543
Size: 82 1052 759 1170 480
Fragments arranged by size:
1170 1052 759 480 82
PspXI vC'TCGA_Gb
Cuts at: 0 1478 3543
Size: 1478 2065
PstI C_TGCA'G
Cuts at: 0 549 1388 1394 2720 2891 3543
Size: 549 839 6 1326 171 652
Fragments arranged by size:
1326 839 652 549 171 6
PvuII CAG'CTG
Cuts at: 0 544 1389 2298 3543
Size: 544 845 909 1245
Fragments arranged by size:
1245 909 845 544
SacI G_AGCT'C
Cuts at: 0 136 1625 3543
Size: 136 1489 1918
Fragments arranged by size:
1918 1489 136
SapI GCTCTTCn'nnn_
Cuts at: 0 3139 3543
Size: 3139 404
ScaI AGT'ACT
Cuts at: 0 1678 3543
Size: 1678 1865
SexAI A'CCwGG_T
Cuts at: 0 3326 3543
Size: 3326 217
SfcI C'TryA_G
Cuts at: 0 545 1384 1390 2716 2880 2887 3543
Size: 545 839 6 1326 164 7 656
Fragments arranged by size:
1326 839 656 545 164 7 6
SmlI C'TyrA_G
Cuts at: 0 1192 1267 1478 2024 2694 3126 3472 3543
Size: 1192 75 211 546 670 432 346 71
Fragments arranged by size:
1192 670 546 432 346 211 75 71
StuI AGG'CCT
Cuts at: 0 1442 3543
Size: 1442 2101
StyI C'CwwG_G
Cuts at: 0 480 1796 3287 3543
Size: 480 1316 1491 256
Fragments arranged by size:
1491 1316 480 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2293 2297 2497 2546 3163 3543
Size: 2293 4 200 49 617 380
Fragments arranged by size:
2293 617 380 200 49 4
TatI w'GTAC_w
Cuts at: 0 1676 1724 3543
Size: 1676 48 1819
Fragments arranged by size:
1819 1676 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1543 1575 1877 1909 2499 2531 2642 2674
Size: 1543 32 302 32 590 32 111 32
Cuts at: 2674 2826 2858 3543
Size: 152 32 685
Fragments arranged by size:
1543 685 590 302 152 111 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2826 3324 3543
Size: 2826 498 219
Fragments arranged by size:
2826 498 219
XhoI C'TCGA_G
Cuts at: 0 1478 3126 3543
Size: 1478 1648 417
Fragments arranged by size:
1648 1478 417
XmnI GAAnn'nnTTC
Cuts at: 0 227 1531 1846 3340 3543
Size: 227 1304 315 1494 203
Fragments arranged by size:
1494 1304 315 227 203
Enzymes that do cut:
AarI AclI AcuI AfeI AflIII AgeI AleI AlfI
AloI AlwNI ApoI AvaI BanI BanII BbsI BbvCI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstYI BtgI BtgZI BtsI CspCI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI
NspI PasI PflMI PfoI PmlI PpuMI PspXI PstI
PvuII SacI SapI ScaI SexAI SfcI SmlI StuI
StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AflII AhdI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BglI BlpI BmtI BsaBI BsiEI BsiWI
BspEI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I ClaI DraI EagI EciI EcoRI EcoRV FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XmaI ZraI