Restriction map for
pFN21AB8956
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# NO CUT ENZYMES ( 127 enzymes) #
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AccI AccIII AclI AcsI AflII
AgeI AhdI AlwNI AocI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvrII BalI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BsrI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
Ksp632I MaeIII MamI MfeI MluI
MluNI MroI MscI MslI MunI
NcoI NdeI NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 27 GACGTC GACGT^C
Acc65I 226 GGTACC G^GTACC
Alw44I 512 GTGCAC G^TGCAC
ApaI 697 GGGCCC GGGCC^C
ApaLI 512 GTGCAC G^TGCAC
Asp718I 226 GGTACC G^GTACC
AspHI 512 GTGCAC G[AT]GC[AT]^C
AvaII 482 GGACC G^G[AT]CC
AviII 976 TGCGCA TGC^GCA
BamHI 361 GGATCC G^GATCC
BbrPI 517 CACGTG CAC^GTG
BbsI 652 GTCTTC GAAGAC(2/6)
BfaI 412 CTAG C^TAG
BlpI 288 GCTCAGC GC^T[ACGT]AGC
BpmI 661 CTGGAG CTGGAG(16/14)
Bpu1102I 288 GCTCAGC GC^T[ACGT]AGC
BpuAI 652 GTCTTC GAAGAC(2/6)
BsaAI 517 CACGTG [CT]AC^GT[GA]
BsgI 531 GTGCAG GTGCAG(16/14)
BsiEI 468 CGACCG CG[GA][CT]^CG
BsiHKAI 512 GTGCAC G[AT]GC[AT]^C
BssHII 705 GCGCGC G^CGCGC
BstF5I 39 CATCC GGATG(2/0)
BstXI 830 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 361 GGATCC [GA]^GATC[CT]
CelII 288 GCTCAGC GC^T[ACGT]AGC
EcoNI 411 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 478 CCAAGG C^C[AT][AT]GG
FokI 39 CATCC GGATG(9/13)
FseI 345 GGCCGGCC GGCCGG^CC
FspI 976 TGCGCA TGC^GCA
KpnI 226 GGTACC GGTAC^C
MaeI 412 CTAG C^TAG
MboII 653 TCTTC GAAGA(8/7)
MflI 361 GGATCC [GA]^GATC[CT]
MseI 674 TTAA T^TAA
PmaCI 517 CACGTG CAC^GTG
PmlI 517 CACGTG CAC^GTG
PstI 919 CTGCAG CTGCA^G
SfaNI 121 GCATC GCATC(5/9)
SfcI 919 CTGCAG C^T[GA][CT]AG
SfiI 336 GGCCAGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 106 CCCGGG CCC^GGG
SmlI 366 CTTGAG C^T[CT][GA]AG
StyI 478 CCAAGG C^C[AT][AT]GG
TaqI 656 TCGA T^CGA
Tru9I 674 TTAA T^TAA
TspRI 917 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 656 TCGA T^CGA
XhoII 361 GGATCC [GA]^GATC[CT]
XmaCI 106 CCCGGG C^CCGGG
XmaI 106 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 227 GTAC GT^AC
942 GTAC
AflIII 516 ACACGT A^C[GA][CT]GT
518 ACGTGT
Aor51HI 620 AGCGCT AGC^GCT
683 AGCGCT
AvaI 106 CCCGGG C^[CT]CG[GA]G
700 CCCGAG
BanII 258 GAGCCC G[GA]GC[CT]^C
697 GGGCCC
BsmFI 173 GGGAC GGGAC(10/14)
272 GGGAC
BsoBI 106 CCCGGG C^[CT]CG[GA]G
700 CCCGAG
DdeI 289 CTCAG C^T[ACGT]AG
462 CTCAG
DpnII 48 GATC ^GATC
362 GATC
DsaI 131 CCGCGG C^C[GA][CT]GG
425 CCGCGG
Eco47III 620 AGCGCT AGC^GCT
683 AGCGCT
HphI 747 GGTGA GGTGA(8/7)
734 TCACC
KspI 131 CCGCGG CCGC^GG
425 CCGCGG
MaeII 28 ACGT A^CGT
518 ACGT
MboI 48 GATC ^GATC
362 GATC
NdeII 48 GATC ^GATC
362 GATC
PvuII 236 CAGCTG CAG^CTG
980 CAGCTG
RsaI 227 GTAC GT^AC
942 GTAC
SacII 131 CCGCGG CCGC^GG
425 CCGCGG
Sau3AI 48 GATC ^GATC
362 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
BanI BbeI BbvI BcnI BglI
BmyI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmBI Bsp1286I BspMI
BsrFI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NgoMIV NlaIV PleI
Sau96I ScrFI SfoI TseI