(Circular) (Six-base) MAPSORT of: pFN21AB8956.seq Check: 5502 from: 1 to: 5704 pFN21AB8956 5704 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2740 2987 2740 Size: 247 5457 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2036 3514 278 Size: 53 83 186 936 500 1478 2468 Fragments arranged by size: 2468 1478 936 500 186 83 53 AccI GT'mk_AC Cuts at: 1937 3047 1937 Size: 1110 4594 Acc65I G'GTAC_C Cuts at: 2231 3025 2231 Size: 794 4910 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2753 3767 5151 5419 1632 Size: 1121 1014 1384 268 1917 Fragments arranged by size: 1917 1384 1121 1014 268 AfeI AGC'GCT Cuts at: 2627 2690 2627 Size: 63 5641 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 2521 2523 4603 5580 1203 Size: 1318 2 2080 977 1327 Fragments arranged by size: 2080 1327 1318 977 2 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 4433 4433 Size: 5704 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5704 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5704 AlwNI CAG_nnn'CTG Cuts at: 3447 5019 3447 Size: 1572 4132 ApaI G_GGCC'C Cuts at: 2706 2706 Size: 5704 ApaLI G'TGCA_C Cuts at: 2517 3761 4917 2517 Size: 1244 1156 3304 Fragments arranged by size: 3304 1244 1156 ApoI r'AATT_y Cuts at: 1493 3013 3239 3391 5500 1493 Size: 1520 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 226 152 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 2111 2705 3029 1870 Size: 73 168 594 324 4545 Fragments arranged by size: 4545 594 324 168 73 BamHI G'GATC_C Cuts at: 2366 3034 3401 2366 Size: 668 367 4669 Fragments arranged by size: 4669 668 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2100 2107 2198 2231 Size: 325 217 627 231 82 7 91 33 Cuts at: 2231 2605 2824 2996 3025 4480 618 Size: 374 219 172 29 1455 1842 Fragments arranged by size: 1842 1455 627 374 325 231 219 217 172 91 82 33 29 7 BanII G_rGCy'C Cuts at: 1391 2267 2706 3023 1391 Size: 876 439 317 4072 Fragments arranged by size: 4072 876 439 317 BbeI G_GCGC'C Cuts at: 2022 2104 2111 2202 2609 2828 2022 Size: 82 7 91 407 219 4898 Fragments arranged by size: 4898 407 219 91 82 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2650 928 Size: 971 751 3982 Fragments arranged by size: 3982 971 751 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3895 3929 1903 Size: 34 1958 34 3678 Fragments arranged by size: 3678 1958 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 1548 Size: 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2098 2271 2348 Size: 107 122 71 631 605 426 173 77 Cuts at: 2348 2961 4315 136 Size: 613 1354 1525 Fragments arranged by size: 1525 1354 631 613 605 426 173 122 107 77 71 BglII A'GATC_T Cuts at: 5699 5699 Size: 5704 BlpI GC'TnA_GC Cuts at: 2294 3121 2294 Size: 827 4877 Bme1580I G_kGCm'C Cuts at: 1790 2120 2521 2706 3765 4921 1790 Size: 330 401 185 1059 1156 2573 Fragments arranged by size: 2573 1156 1059 401 330 185 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3435 4393 442 Size: 416 53 83 399 24 2018 958 1753 Fragments arranged by size: 2018 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2687 4364 1239 Size: 1448 1677 2579 Fragments arranged by size: 2579 1677 1448 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2392 3829 4694 4992 2392 Size: 1437 865 298 3104 Fragments arranged by size: 3104 1437 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4367 882 Size: 617 2868 2219 Fragments arranged by size: 2868 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2524 3495 493 Size: 711 1320 971 2702 Fragments arranged by size: 2702 1320 971 711 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 2033 2101 2108 Size: 53 83 186 936 486 14 68 7 Cuts at: 2108 2199 2219 2577 2606 2825 3511 3893 4528 Size: 91 20 358 29 219 686 382 635 Cuts at: 4528 275 Size: 1451 Fragments arranged by size: 1451 936 686 635 486 382 358 219 186 91 83 68 53 29 20 14 7 BsaWI w'CCGG_w Cuts at: 4137 4535 4809 4956 5367 4137 Size: 398 274 147 411 4474 Fragments arranged by size: 4474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 1178 Size: 30 165 30 867 30 4582 Fragments arranged by size: 4582 867 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 2315 2435 2600 2603 2606 2609 1186 Size: 898 231 120 165 3 3 3 4281 Fragments arranged by size: 4281 898 231 165 120 3 3 3 BseYI C'CCAG_C Cuts at: 2274 2964 4907 5403 2274 Size: 690 1943 496 2575 Fragments arranged by size: 2575 1943 690 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2557 1163 Size: 573 821 4310 Fragments arranged by size: 4310 821 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2476 3154 3915 4943 1540 Size: 275 186 475 678 761 1028 2301 Fragments arranged by size: 2301 1028 761 678 475 275 186 BsiHKAI G_wGCw'C Cuts at: 2521 3765 3850 4921 2521 Size: 1244 85 1071 3304 Fragments arranged by size: 3304 1244 1071 85 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 BsmBI CGTCTCn'nnnn_ Cuts at: 2226 2470 2738 2226 Size: 244 268 5192 Fragments arranged by size: 5192 268 244 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2267 2521 2706 3023 3765 3850 Size: 399 330 147 254 185 317 742 85 Cuts at: 3850 4921 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 330 317 254 185 147 85 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 3593 5323 3593 Size: 1730 3974 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2644 2740 2820 2892 2987 3059 844 Size: 1800 96 80 72 95 72 3489 Fragments arranged by size: 3489 1800 96 95 80 72 72 BsrBI CCG'CTC Cuts at: 1394 1823 3591 1394 Size: 429 1768 3507 Fragments arranged by size: 3507 1768 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2228 2351 2386 2546 2569 2599 2793 Size: 110 418 123 35 160 23 30 194 Cuts at: 2793 4348 4535 1700 Size: 1555 187 2869 Fragments arranged by size: 2869 1555 418 194 187 160 123 110 35 30 23 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5704 BssHII G'CGCG_C Cuts at: 1925 2710 1925 Size: 785 4919 BssSI C'ACGA_G Cuts at: 3764 4776 3764 Size: 1012 4692 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5704 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstXI CCAn_nnnn'nTGG Cuts at: 2842 2842 Size: 5704 BstYI r'GATC_y Cuts at: 1972 2366 3034 3401 3786 3803 5255 5341 5353 Size: 394 668 367 385 17 1452 86 12 Cuts at: 5353 5699 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 668 394 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 2430 513 Size: 552 939 132 294 3787 Fragments arranged by size: 3787 939 552 294 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 2921 3220 4014 4034 2921 Size: 299 794 20 4591 Fragments arranged by size: 4591 794 299 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4711 809 Size: 507 3395 1802 Fragments arranged by size: 3395 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2133 2380 Size: 54 1425 50 275 18 255 48 247 Cuts at: 2380 2400 2721 2844 2903 3151 4040 5669 8 Size: 20 321 123 59 248 889 1629 43 Fragments arranged by size: 1629 1425 889 321 275 255 248 247 123 59 54 50 48 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 3151 1537 Size: 275 1339 4090 Fragments arranged by size: 4090 1339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3634 1353 Size: 2281 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2446 4291 4665 4811 387 Size: 1064 138 857 1845 374 146 1280 Fragments arranged by size: 1845 1280 1064 857 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2687 2753 3767 4364 5151 5419 1239 Size: 393 1055 66 1014 597 787 268 1524 Fragments arranged by size: 1524 1055 1014 787 597 393 268 66 EcoNI CCTnn'n_nnAGG Cuts at: 2420 2420 Size: 5704 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FseI GG_CCGG'CC Cuts at: 2355 2355 Size: 5704 FspI TGC'GCA Cuts at: 2983 4210 2983 Size: 1227 4477 HaeII r_GCGC'y Cuts at: 1678 2022 2104 2111 2202 2323 2609 2629 2674 Size: 344 82 7 91 121 286 20 45 Cuts at: 2674 2692 2794 2828 2972 4851 1678 Size: 18 102 34 144 1879 2531 Fragments arranged by size: 2531 1879 344 286 144 121 102 91 82 45 34 20 18 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2270 2302 4345 4377 4419 Size: 32 135 32 698 32 2043 32 42 Cuts at: 4419 4451 1373 Size: 32 2626 Fragments arranged by size: 2626 2043 698 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3048 3294 669 Size: 988 281 1110 246 3079 Fragments arranged by size: 3079 1110 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KasI G'GCGC_C Cuts at: 2018 2100 2107 2198 2605 2824 2018 Size: 82 7 91 407 219 4898 Fragments arranged by size: 4898 407 219 91 82 7 KpnI G_GTAC'C Cuts at: 2235 3029 2235 Size: 794 4910 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4818 5002 1305 Size: 3513 184 2007 Fragments arranged by size: 3513 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3882 Fragments arranged by size: 3882 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3662 4021 4180 518 Size: 648 843 1653 359 159 2042 Fragments arranged by size: 2042 1653 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2123 2138 2147 2243 2346 2432 2541 2565 Size: 517 15 9 96 103 86 109 24 Cuts at: 2565 2987 3120 3797 4945 5190 1606 Size: 422 133 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 517 422 245 133 109 103 96 86 24 15 9 NaeI GCC'GGC Cuts at: 2353 2548 2571 2601 2795 2353 Size: 195 23 30 194 5262 Fragments arranged by size: 5262 195 194 30 23 NarI GG'CG_CC Cuts at: 2019 2101 2108 2199 2606 2825 2019 Size: 82 7 91 407 219 4898 Fragments arranged by size: 4898 407 219 91 82 7 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4213 Fragments arranged by size: 4213 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NgoMIV G'CCGG_C Cuts at: 2351 2546 2569 2599 2793 2351 Size: 195 23 30 194 5262 Fragments arranged by size: 5262 195 194 30 23 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 3062 4607 1132 Size: 97 1833 1545 2229 Fragments arranged by size: 2229 1833 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5704 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PmlI CAC'GTG Cuts at: 2524 2524 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PspOMI G'GGCC_C Cuts at: 2702 2702 Size: 5704 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2762 2794 2762 Size: 32 5672 PstI C_TGCA'G Cuts at: 830 2928 3056 830 Size: 2098 128 3478 Fragments arranged by size: 3478 2098 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 PvuII CAG'CTG Cuts at: 2243 2987 2243 Size: 744 4960 SacII CC_GC'GG Cuts at: 2139 2433 2139 Size: 294 5410 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 3952 5664 3952 Size: 1712 3992 SfcI C'TryA_G Cuts at: 826 1045 2924 3052 4187 4868 5059 826 Size: 219 1879 128 1135 681 191 1471 Fragments arranged by size: 1879 1471 1135 681 219 191 128 SfiI GGCCn_nnn'nGGCC Cuts at: 2348 2348 Size: 5704 SfoI GGC'GCC Cuts at: 2020 2102 2109 2200 2607 2826 2020 Size: 82 7 91 407 219 4898 Fragments arranged by size: 4898 407 219 91 82 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 2113 3031 1872 Size: 241 918 4545 Fragments arranged by size: 4545 918 241 SmlI C'TyrA_G Cuts at: 820 1017 1943 2371 3808 4709 4971 820 Size: 197 926 428 1437 901 262 1553 Fragments arranged by size: 1553 1437 926 901 428 262 197 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5704 StyI C'CwwG_G Cuts at: 513 1065 2004 2483 3143 513 Size: 552 939 479 660 3074 Fragments arranged by size: 3074 939 660 552 479 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2504 3014 3731 3748 3901 4086 4531 Size: 1255 924 510 717 17 153 185 445 Cuts at: 4531 325 Size: 1498 Fragments arranged by size: 1498 1255 924 717 510 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3950 5662 96 Size: 274 80 33 51 1443 1973 1712 138 Fragments arranged by size: 1973 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5664 5696 5664 Size: 32 5672 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 2111 3029 1870 Size: 241 918 4545 Fragments arranged by size: 4545 918 241 XmnI GAAnn'nnTTC Cuts at: 3833 3833 Size: 5704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2034 3512 276 Size: 53 83 186 936 500 1478 2468 Fragments arranged by size: 2468 1478 936 500 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FspAI NruI NsiI PacI PfoI PpuMI PspXI RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI