(Circular) (Six-base) MAPSORT of: pFN21AB8902.seq Check: 1226 from: 1 to: 5407 pFN21AB8902 5407 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3217 278 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 1937 Size: 813 4594 Acc65I G'GTAC_C Cuts at: 2728 2728 Size: 5407 AclI AA'CG_TT Cuts at: 1563 3534 3907 1563 Size: 1971 373 3063 Fragments arranged by size: 3063 1971 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2316 3470 4854 5122 1632 Size: 684 1154 1384 268 1917 Fragments arranged by size: 1917 1384 1154 684 268 AfeI AGC'GCT Cuts at: 2381 2381 Size: 5407 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5210 AflIII A'CryG_T Cuts at: 1203 4306 5283 1203 Size: 3103 977 1327 Fragments arranged by size: 3103 1327 977 AgeI A'CCGG_T Cuts at: 4238 4238 Size: 5407 AhdI GACnn_n'nnGTC Cuts at: 4136 4136 Size: 5407 AleI CACnn'nnGTG Cuts at: 1166 2408 1166 Size: 1242 4165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5407 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2414 2446 2414 Size: 32 5375 AlwNI CAG_nnn'CTG Cuts at: 3150 4722 3150 Size: 1572 3835 ApaI G_GGCC'C Cuts at: 2244 2470 2244 Size: 226 5181 ApaLI G'TGCA_C Cuts at: 3464 4620 3464 Size: 1156 4251 ApoI r'AATT_y Cuts at: 1493 2716 2942 3094 5203 1493 Size: 1223 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 226 152 AseI AT'TA_AT Cuts at: 160 1032 3961 160 Size: 872 2929 1606 Fragments arranged by size: 2929 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2385 2622 2732 1870 Size: 73 87 355 237 110 4545 Fragments arranged by size: 4545 355 237 110 87 73 BamHI G'GATC_C Cuts at: 2737 3104 2737 Size: 367 5040 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2336 2728 4183 618 Size: 325 217 627 549 392 1455 1842 Fragments arranged by size: 1842 1455 627 549 392 325 217 BanII G_rGCy'C Cuts at: 1391 2244 2470 2696 2726 1391 Size: 853 226 226 30 4072 Fragments arranged by size: 4072 853 226 226 30 BbeI G_GCGC'C Cuts at: 2340 2340 Size: 5407 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3598 3632 1903 Size: 34 50 34 1577 34 3678 Fragments arranged by size: 3678 1577 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3299 4509 3299 Size: 1210 4197 BclI T'GATC_A Cuts at: 1548 1548 Size: 5407 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2293 2474 4018 Size: 107 122 71 631 605 621 181 1544 Cuts at: 4018 136 Size: 1525 Fragments arranged by size: 1544 1525 631 621 605 181 122 107 71 BglII A'GATC_T Cuts at: 5402 5402 Size: 5407 BlpI GC'TnA_GC Cuts at: 2231 2824 2231 Size: 593 4814 Bme1580I G_kGCm'C Cuts at: 1790 2244 2470 3468 4624 1790 Size: 454 226 998 1156 2573 Fragments arranged by size: 2573 1156 998 454 226 BmgBI CAC'GTC Cuts at: 1318 2085 1318 Size: 767 4640 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3138 4096 442 Size: 416 53 83 399 24 1721 958 1753 Fragments arranged by size: 1753 1721 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5407 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2602 2635 2602 Size: 33 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2710 4067 1239 Size: 1471 1357 2579 Fragments arranged by size: 2579 1471 1357 Bpu10I CC'TnA_GC Cuts at: 2009 2364 2009 Size: 355 5052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2471 3532 4397 4695 2471 Size: 1061 865 298 3183 Fragments arranged by size: 3183 1061 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2273 2492 2641 4070 882 Size: 617 774 219 149 1429 2219 Fragments arranged by size: 2219 1429 774 617 219 149 BsaAI yAC'GTr Cuts at: 493 1204 3198 493 Size: 711 1994 2702 Fragments arranged by size: 2702 1994 711 BsaBI GATnn'nnATC Cuts at: 5401 5401 Size: 5407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2337 3214 3596 4231 Size: 53 83 186 936 804 877 382 635 Cuts at: 4231 275 Size: 1451 Fragments arranged by size: 1451 936 877 804 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2017 3840 4238 4512 4659 5070 2017 Size: 1823 398 274 147 411 2354 Fragments arranged by size: 2354 1823 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2414 2444 1178 Size: 30 165 30 1011 30 4141 Fragments arranged by size: 4141 1011 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2273 2548 2578 2624 1186 Size: 1087 275 30 46 3969 Fragments arranged by size: 3969 1087 275 46 30 BseYI C'CCAG_C Cuts at: 2566 2695 4610 5106 2566 Size: 129 1915 496 2867 Fragments arranged by size: 2867 1915 496 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4834 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2137 2857 3618 4646 1540 Size: 275 186 136 720 761 1028 2301 Fragments arranged by size: 2301 1028 761 720 275 186 136 BsiHKAI G_wGCw'C Cuts at: 2237 3468 3553 4624 2237 Size: 1231 85 1071 3020 Fragments arranged by size: 3020 1231 1071 85 BsmI GAATG_Cn' Cuts at: 2918 3011 2918 Size: 93 5314 BsmBI CGTCTCn'nnnn_ Cuts at: 2261 2261 Size: 5407 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2244 2470 2696 2726 3468 3553 Size: 399 447 7 226 226 30 742 85 Cuts at: 3553 4624 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 226 226 85 30 7 BspEI T'CCGG_A Cuts at: 2017 5070 2017 Size: 3053 2354 BspHI T'CATG_A Cuts at: 3296 5026 3296 Size: 1730 3677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2762 844 Size: 1918 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2080 3294 1394 Size: 429 257 1214 3507 Fragments arranged by size: 3507 1214 429 257 BsrDI GCAATG_nn' Cuts at: 65 1248 3902 4076 65 Size: 1183 2654 174 1396 Fragments arranged by size: 2654 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2596 4051 4238 1700 Size: 110 786 1455 187 2869 Fragments arranged by size: 2869 1455 786 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5407 BssHII G'CGCG_C Cuts at: 1925 2118 1925 Size: 193 5214 BssSI C'ACGA_G Cuts at: 2140 3467 4479 2140 Size: 1327 1012 3068 Fragments arranged by size: 3068 1327 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5407 BstBI TT'CG_AA Cuts at: 4211 4211 Size: 5407 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5407 BstXI CCAn_nnnn'nTGG Cuts at: 2703 2703 Size: 5407 BstYI r'GATC_y Cuts at: 1972 2737 3104 3489 3506 4958 5044 5056 5402 Size: 765 367 385 17 1452 86 12 346 Cuts at: 5402 1972 Size: 1977 Fragments arranged by size: 1977 1452 765 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2024 513 Size: 552 959 3896 Fragments arranged by size: 3896 959 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4668 Fragments arranged by size: 4668 683 56 BtsI GCAGTG_nn' Cuts at: 2923 3717 3737 2923 Size: 794 20 4593 Fragments arranged by size: 4593 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5372 DraI TTT'AAA Cuts at: 2711 2711 Size: 5407 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4414 809 Size: 507 3098 1802 Fragments arranged by size: 3098 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2134 2475 2854 Size: 54 1425 50 275 18 304 341 379 Cuts at: 2854 3743 5372 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 379 341 304 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2134 2854 1537 Size: 275 322 720 4090 Fragments arranged by size: 4090 720 322 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3337 1353 Size: 1984 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3994 4368 4514 387 Size: 1064 138 2405 374 146 1280 Fragments arranged by size: 2405 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2316 2710 3470 4067 4854 5122 1239 Size: 393 684 394 760 597 787 268 1524 Fragments arranged by size: 1524 787 760 684 597 394 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2096 2606 2096 Size: 510 4897 EcoO109I rG'GnC_Cy Cuts at: 2241 2241 Size: 5407 EcoRI G'AATT_C Cuts at: 2716 3094 2716 Size: 378 5029 FspI TGC'GCA Cuts at: 3913 3913 Size: 5407 HaeII r_GCGC'y Cuts at: 1678 2340 2383 4554 1678 Size: 662 43 2171 2531 Fragments arranged by size: 2531 2171 662 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373 Size: 32 135 32 2476 32 42 32 2626 Fragments arranged by size: 2626 2476 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2751 2997 669 Size: 988 281 813 246 3079 Fragments arranged by size: 3079 988 813 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5407 HpaI GTT'AAC Cuts at: 2997 2997 Size: 5407 KasI G'GCGC_C Cuts at: 2336 2336 Size: 5407 KpnI G_GTAC'C Cuts at: 2732 2732 Size: 5407 MfeI C'AATT_G Cuts at: 3006 3006 Size: 5407 MluI A'CGCG_T Cuts at: 5283 5283 Size: 5407 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2295 2400 4521 4705 1305 Size: 990 105 2121 184 2007 Fragments arranged by size: 2121 2007 990 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 2477 10 Size: 54 1425 343 645 2940 Fragments arranged by size: 2940 1425 645 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 3365 3724 3883 518 Size: 648 1242 957 359 159 2042 Fragments arranged by size: 2042 1242 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2125 2230 2688 2823 3500 4648 4893 Size: 420 99 105 458 135 677 1148 245 Cuts at: 4893 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 458 420 245 135 105 99 NaeI GCC'GGC Cuts at: 2598 2598 Size: 5407 NarI GG'CG_CC Cuts at: 2337 2337 Size: 5407 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4855 NdeI CA'TA_TG Cuts at: 387 387 Size: 5407 NgoMIV G'CCGG_C Cuts at: 2596 2596 Size: 5407 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5407 NotI GC'GGCC_GC Cuts at: 2134 2854 2134 Size: 720 4687 NspI r_CATG'y Cuts at: 1132 1229 2765 4310 1132 Size: 97 1536 1545 2229 Fragments arranged by size: 2229 1545 1536 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5407 PciI A'CATG_T Cuts at: 4306 4306 Size: 5407 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5407 PfoI T'CCnGG_A Cuts at: 2097 2097 Size: 5407 PmeI GTTT'AAAC Cuts at: 2711 2711 Size: 5407 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2414 2446 3476 3508 4989 5021 2414 Size: 32 1030 32 1481 32 2800 Fragments arranged by size: 2800 1481 1030 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5407 PsiI TTA'TAA Cuts at: 2977 2977 Size: 5407 PspOMI G'GGCC_C Cuts at: 2240 2466 2240 Size: 226 5181 PstI C_TGCA'G Cuts at: 830 2759 830 Size: 1929 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5407 PvuII CAG'CTG Cuts at: 2230 2688 2230 Size: 458 4949 SacII CC_GC'GG Cuts at: 2027 2027 Size: 5407 SalI G'TCGA_C Cuts at: 1936 2749 1936 Size: 813 4594 SbfI CC_TGCA'GG Cuts at: 2759 2759 Size: 5407 ScaI AGT'ACT Cuts at: 3655 5367 3655 Size: 1712 3695 SfcI C'TryA_G Cuts at: 826 1045 2755 3890 4571 4762 826 Size: 219 1710 1135 681 191 1471 Fragments arranged by size: 1710 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2474 2474 Size: 5407 SfoI GGC'GCC Cuts at: 2338 2338 Size: 5407 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5407 SmaI CCC'GGG Cuts at: 1872 2032 2624 2734 1872 Size: 160 592 110 4545 Fragments arranged by size: 4545 592 160 110 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 3511 4412 4674 820 Size: 197 926 543 1025 901 262 1553 Fragments arranged by size: 1553 1025 926 901 543 262 197 SnaBI TAC'GTA Cuts at: 493 3198 493 Size: 2705 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5407 SphI G_CATG'C Cuts at: 1132 2765 1132 Size: 1633 3774 SspI AAT'ATT Cuts at: 5 52 3331 5 Size: 47 3279 2081 Fragments arranged by size: 3279 2081 47 StuI AGG'CCT Cuts at: 1517 2615 1517 Size: 1098 4309 StyI C'CwwG_G Cuts at: 513 1065 2345 2846 513 Size: 552 1280 501 3074 Fragments arranged by size: 3074 1280 552 501 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 3434 3451 3604 3789 4234 325 Size: 1255 717 1137 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1137 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 3653 5365 Size: 274 80 33 51 1443 696 980 1712 Cuts at: 5365 96 Size: 138 Fragments arranged by size: 1712 1443 980 696 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5367 5399 5367 Size: 32 5375 Tth111I GACn'n_nGTC Cuts at: 1316 2068 1316 Size: 752 4655 XbaI T'CTAG_A Cuts at: 2743 2743 Size: 5407 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5407 XmaI C'CCGG_G Cuts at: 1870 2030 2622 2732 1870 Size: 160 592 110 4545 Fragments arranged by size: 4545 592 160 110 XmnI GAAnn'nnTTC Cuts at: 3536 3536 Size: 5407 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3215 276 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI