(Circular) (Six-base) MAPSORT of: pFN21AB8950.seq Check: 2502 from: 1 to: 5677 pFN21AB8950 5677 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2487 2998 2487 Size: 511 5166 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3740 5124 5392 1632 Size: 2108 1384 268 1917 Fragments arranged by size: 2108 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 4576 5553 1203 Size: 3373 977 1327 Fragments arranged by size: 3373 1327 977 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AlwNI CAG_nnn'CTG Cuts at: 2608 3420 4992 2608 Size: 812 1572 3293 Fragments arranged by size: 3293 1572 812 ApaI G_GGCC'C Cuts at: 2181 2181 Size: 5677 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2986 3212 3364 5473 1493 Size: 1493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1493 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2180 2403 2921 2941 3002 1870 Size: 73 237 223 518 20 61 4545 Fragments arranged by size: 4545 518 237 223 73 61 20 AvrII C'CTAG_G Cuts at: 2695 2695 Size: 5677 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2128 2487 2662 2707 2998 Size: 325 217 627 341 359 175 45 291 Cuts at: 2998 4453 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 359 341 325 291 217 175 45 BanII G_rGCy'C Cuts at: 1391 2103 2181 2921 2996 1391 Size: 712 78 740 75 4072 Fragments arranged by size: 4072 740 712 78 75 BbeI G_GCGC'C Cuts at: 2132 2711 2132 Size: 579 5098 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2381 928 Size: 971 482 4224 Fragments arranged by size: 4224 971 482 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2171 2205 3868 3902 1903 Size: 34 234 34 1663 34 3678 Fragments arranged by size: 3678 1663 234 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2975 3569 4779 2975 Size: 594 1210 3873 Fragments arranged by size: 3873 1210 594 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2553 5672 2553 Size: 3119 2558 BlpI GC'TnA_GC Cuts at: 3094 3094 Size: 5677 Bme1580I G_kGCm'C Cuts at: 1790 2181 3738 4894 1790 Size: 391 1557 1156 2573 Fragments arranged by size: 2573 1557 1156 391 BmgBI CAC'GTC Cuts at: 1318 2538 1318 Size: 1220 4457 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3408 4366 442 Size: 416 53 83 399 24 1991 958 1753 Fragments arranged by size: 1991 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2390 2483 2630 4337 1239 Size: 896 255 93 147 1707 2579 Fragments arranged by size: 2579 1707 896 255 147 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3802 4667 4965 3802 Size: 865 298 4514 Fragments arranged by size: 4514 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 882 Size: 617 2841 2219 Fragments arranged by size: 2841 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2288 3468 493 Size: 711 1084 1180 2702 Fragments arranged by size: 2702 1180 1084 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2129 2708 2892 3484 Size: 53 83 186 936 596 579 184 592 Cuts at: 3484 3866 4501 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 596 592 579 382 186 184 83 53 BsaWI w'CCGG_w Cuts at: 4110 4508 4782 4929 5340 4110 Size: 398 274 147 411 4447 Fragments arranged by size: 4447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5452 Fragments arranged by size: 5452 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2125 1186 Size: 939 4738 BseYI C'CCAG_C Cuts at: 2012 2174 2221 2308 2842 4880 5376 2012 Size: 162 47 87 534 2038 496 2313 Fragments arranged by size: 2313 2038 534 496 162 87 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2797 1163 Size: 573 1061 4043 Fragments arranged by size: 4043 1061 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3888 4916 1540 Size: 275 186 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2103 2436 3738 3823 4894 2103 Size: 333 1302 85 1071 2886 Fragments arranged by size: 2886 1302 1071 333 85 BsmI GAATG_Cn' Cuts at: 2911 3188 3281 2911 Size: 277 93 5307 Fragments arranged by size: 5307 277 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2181 2436 2921 2996 3738 3823 Size: 399 313 78 255 485 75 742 85 Cuts at: 3823 4894 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 485 399 313 255 85 78 75 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2440 2536 3032 844 Size: 1596 96 496 3489 Fragments arranged by size: 3489 1596 496 96 BsrBI CCG'CTC Cuts at: 1394 1823 2398 2467 3564 1394 Size: 429 575 69 1097 3507 Fragments arranged by size: 3507 1097 575 429 69 BsrDI GCAATG_nn' Cuts at: 65 1248 4172 4346 65 Size: 1183 2924 174 1396 Fragments arranged by size: 2924 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2239 2568 2851 4321 4508 1700 Size: 110 429 329 283 1470 187 2869 Fragments arranged by size: 2869 1470 429 329 283 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 2212 2229 2346 1925 Size: 287 17 117 5256 Fragments arranged by size: 5256 287 117 17 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 2691 794 Size: 1897 3780 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstYI r'GATC_y Cuts at: 1972 2553 3007 3374 3759 3776 5228 5314 5326 Size: 581 454 367 385 17 1452 86 12 Cuts at: 5326 5672 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 581 454 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2694 487 Size: 683 56 1468 3470 Fragments arranged by size: 3470 1468 683 56 BtsI GCAGTG_nn' Cuts at: 2814 2856 3193 3987 4007 2814 Size: 42 337 794 20 4484 Fragments arranged by size: 4484 794 337 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2241 2517 Size: 54 1425 50 275 18 318 93 276 Cuts at: 2517 2853 3124 4013 5642 8 Size: 336 271 889 1629 43 Fragments arranged by size: 1629 1425 889 336 318 276 275 271 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2441 3607 1353 Size: 1088 1166 3423 Fragments arranged by size: 3423 1166 1088 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2399 2431 4264 4638 4784 387 Size: 1064 138 810 32 1833 374 146 1280 Fragments arranged by size: 1833 1280 1064 810 374 146 138 32 EcoICRI GAG'CTC Cuts at: 2101 2101 Size: 5677 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2390 2483 2630 3740 4337 5124 Size: 393 503 255 93 147 1110 597 787 Cuts at: 5124 5392 1239 Size: 268 1524 Fragments arranged by size: 1524 1110 787 597 503 393 268 255 147 93 EcoO109I rG'GnC_Cy Cuts at: 2317 2317 Size: 5677 EcoRI G'AATT_C Cuts at: 2986 3364 2986 Size: 378 5299 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 2132 2473 2575 2711 4824 1678 Size: 454 341 102 136 2113 2531 Fragments arranged by size: 2531 2113 454 341 136 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4318 4350 4392 4424 1373 Size: 32 135 32 2746 32 42 32 2626 Fragments arranged by size: 2746 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3267 669 Size: 988 281 1083 246 3079 Fragments arranged by size: 3079 1083 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KasI G'GCGC_C Cuts at: 2128 2707 2128 Size: 579 5098 KpnI G_GTAC'C Cuts at: 2491 3002 2491 Size: 511 5166 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4791 4975 1305 Size: 3486 184 2007 Fragments arranged by size: 3486 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2519 10 Size: 54 1425 343 687 3168 Fragments arranged by size: 3168 1425 687 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3635 3994 4153 518 Size: 648 2469 359 159 2042 Fragments arranged by size: 2469 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2485 2515 2593 2811 3093 3770 4918 5163 Size: 879 30 78 218 282 677 1148 245 Cuts at: 5163 1606 Size: 2120 Fragments arranged by size: 2120 1148 879 677 282 245 218 78 30 NaeI GCC'GGC Cuts at: 2853 2853 Size: 5677 NarI GG'CG_CC Cuts at: 2129 2708 2129 Size: 579 5098 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5125 NdeI CA'TA_TG Cuts at: 387 387 Size: 5677 NgoMIV G'CCGG_C Cuts at: 2851 2851 Size: 5677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 2146 3035 4580 1132 Size: 97 917 889 1545 2229 Fragments arranged by size: 2229 1545 917 889 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 2608 1108 Size: 1500 4177 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PmlI CAC'GTG Cuts at: 2288 2288 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2317 2317 Size: 5677 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 2754 3247 2754 Size: 493 5184 PspOMI G'GGCC_C Cuts at: 2177 2177 Size: 5677 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2558 2590 2558 Size: 32 5645 PstI C_TGCA'G Cuts at: 830 2355 2798 3029 830 Size: 1525 443 231 3478 Fragments arranged by size: 3478 1525 443 231 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 PvuII CAG'CTG Cuts at: 2515 2811 2515 Size: 296 5381 SacI G_AGCT'C Cuts at: 2103 2103 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SapI GCTCTTCn'nnn_ Cuts at: 2441 2441 Size: 5677 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 3925 5637 3925 Size: 1712 3965 SfcI C'TryA_G Cuts at: 826 1045 2293 2351 2794 2806 3025 4160 4841 Size: 219 1248 58 443 12 219 1135 681 Cuts at: 4841 5032 826 Size: 191 1471 Fragments arranged by size: 1471 1248 1135 681 443 219 219 191 58 12 SfoI GGC'GCC Cuts at: 2130 2709 2130 Size: 579 5098 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 2405 2923 3004 1872 Size: 533 518 81 4545 Fragments arranged by size: 4545 533 518 81 SmlI C'TyrA_G Cuts at: 820 1017 1943 3781 4682 4944 820 Size: 197 926 1838 901 262 1553 Fragments arranged by size: 1838 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 2146 3035 1132 Size: 1014 889 3774 Fragments arranged by size: 3774 1014 889 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 2168 1517 Size: 651 5026 StyI C'CwwG_G Cuts at: 513 1065 2313 2695 3116 513 Size: 552 1248 382 421 3074 Fragments arranged by size: 3074 1248 552 421 382 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3704 3721 3874 4059 4504 325 Size: 1255 2124 17 153 185 445 1498 Fragments arranged by size: 2124 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3923 5635 96 Size: 274 80 33 51 1443 1946 1712 138 Fragments arranged by size: 1946 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5637 5669 5637 Size: 32 5645 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 2403 2921 3002 1870 Size: 533 518 81 4545 Fragments arranged by size: 4545 533 518 81 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI