(Circular) (Six-base) MAPSORT of: pFN21AA1565.seq Check: 8699 from: 1 to: 8530 pFN21AA1565 8530 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4155 4155 Size: 8530 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6340 278 Size: 53 83 186 936 4804 2468 Fragments arranged by size: 4804 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5873 1937 Size: 3936 4594 Acc65I G'GTAC_C Cuts at: 5549 5851 5549 Size: 302 8228 AclI AA'CG_TT Cuts at: 1563 4798 6657 7030 1563 Size: 3235 1859 373 3063 Fragments arranged by size: 3235 3063 1859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2150 2388 3002 3500 3810 4025 4223 4376 Size: 518 238 614 498 310 215 198 153 Cuts at: 4376 4872 5276 6593 7977 8245 1632 Size: 496 404 1317 1384 268 1917 Fragments arranged by size: 1917 1384 1317 614 518 498 496 404 310 268 238 215 198 153 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8333 AflIII A'CryG_T Cuts at: 1203 2118 7429 8406 1203 Size: 915 5311 977 1327 Fragments arranged by size: 5311 1327 977 915 AgeI A'CCGG_T Cuts at: 7361 7361 Size: 8530 AhdI GACnn_n'nnGTC Cuts at: 7259 7259 Size: 8530 AleI CACnn'nnGTG Cuts at: 1166 4823 1166 Size: 3657 4873 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5097 5795 1184 Size: 3913 698 3919 Fragments arranged by size: 3919 3913 698 AlwNI CAG_nnn'CTG Cuts at: 3337 5413 5810 6273 7845 3337 Size: 2076 397 463 1572 4022 Fragments arranged by size: 4022 2076 1572 463 397 ApaI G_GGCC'C Cuts at: 5320 5320 Size: 8530 ApaLI G'TGCA_C Cuts at: 3690 6587 7743 3690 Size: 2897 1156 4477 Fragments arranged by size: 4477 2897 1156 ApoI r'AATT_y Cuts at: 1493 3523 4432 4582 5170 5650 5839 6065 6217 Size: 2030 909 150 588 480 189 226 152 Cuts at: 6217 8326 1493 Size: 2109 1697 Fragments arranged by size: 2109 2030 1697 909 588 480 226 189 152 150 AseI AT'TA_AT Cuts at: 160 1032 2216 7084 160 Size: 872 1184 4868 1606 Fragments arranged by size: 4868 1606 1184 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8530 AvaI C'yCGr_G Cuts at: 1870 1943 2336 2675 5481 5855 1870 Size: 73 393 339 2806 374 4545 Fragments arranged by size: 4545 2806 393 374 339 73 BamHI G'GATC_C Cuts at: 5860 6227 5860 Size: 367 8163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2802 5549 5851 7306 618 Size: 325 217 627 1015 2747 302 1455 1842 Fragments arranged by size: 2747 1842 1455 1015 627 325 302 217 BanII G_rGCy'C Cuts at: 1391 3771 3783 3891 4185 4368 4848 5320 5349 Size: 2380 12 108 294 183 480 472 29 Cuts at: 5349 5849 1391 Size: 500 4072 Fragments arranged by size: 4072 2380 500 480 472 294 183 108 29 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2453 2878 4821 5184 928 Size: 971 554 425 1943 363 4274 Fragments arranged by size: 4274 1943 971 554 425 363 BbvCI CC'TCA_GC Cuts at: 4160 4160 Size: 8530 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6721 6755 1903 Size: 34 4784 34 3678 Fragments arranged by size: 4784 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6422 7632 6422 Size: 1210 7320 BclI T'GATC_A Cuts at: 1548 3220 5599 1548 Size: 1672 2379 4479 Fragments arranged by size: 4479 2379 1672 BfrBI ATG'CAT Cuts at: 2918 2918 Size: 8530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7141 136 Size: 107 122 71 631 605 5469 1525 Fragments arranged by size: 5469 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3169 8525 3169 Size: 5356 3174 BlpI GC'TnA_GC Cuts at: 2286 5947 2286 Size: 3661 4869 Bme1580I G_kGCm'C Cuts at: 1790 2078 3694 5320 6591 7747 1790 Size: 288 1616 1626 1271 1156 2573 Fragments arranged by size: 2573 1626 1616 1271 1156 288 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8530 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2138 6261 7219 Size: 416 53 83 399 24 721 4123 958 Cuts at: 7219 442 Size: 1753 Fragments arranged by size: 4123 1753 958 721 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8530 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2690 2690 Size: 8530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3650 3905 4856 4931 5423 7190 1239 Size: 2411 255 951 75 492 1767 2579 Fragments arranged by size: 2579 2411 1767 951 492 255 75 Bpu10I CC'TnA_GC Cuts at: 3707 4160 3707 Size: 453 8077 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2027 3176 4046 4148 4169 4191 4361 6655 7520 Size: 1149 870 102 21 22 170 2294 865 Cuts at: 7520 7818 2027 Size: 298 2739 Fragments arranged by size: 2739 2294 1149 870 865 298 170 102 22 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2632 7193 882 Size: 617 1133 4561 2219 Fragments arranged by size: 4561 2219 1133 617 BsaAI yAC'GTr Cuts at: 493 1204 6321 493 Size: 711 5117 2702 Fragments arranged by size: 5117 2702 711 BsaBI GATnn'nnATC Cuts at: 4408 8524 4408 Size: 4116 4414 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5826 6337 6719 7354 Size: 53 83 186 936 4293 511 382 635 Cuts at: 7354 275 Size: 1451 Fragments arranged by size: 4293 1451 936 635 511 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2613 6963 7361 7635 7782 8193 2613 Size: 4350 398 274 147 411 2950 Fragments arranged by size: 4350 2950 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8305 Fragments arranged by size: 8305 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 2372 2477 4112 4115 4145 4364 1186 Size: 961 225 105 1635 3 30 219 5352 Fragments arranged by size: 5352 1635 961 225 219 105 30 3 BseYI C'CCAG_C Cuts at: 2419 5313 7733 8229 2419 Size: 2894 2420 496 2720 Fragments arranged by size: 2894 2720 2420 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3655 4829 4913 5207 5791 1163 Size: 573 1919 1174 84 294 584 3902 Fragments arranged by size: 3902 1919 1174 584 573 294 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5980 6741 7769 1540 Size: 275 186 3979 761 1028 2301 Fragments arranged by size: 3979 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2442 3694 3708 3891 4062 4185 4368 4848 6591 Size: 1252 14 183 171 123 183 480 1743 Cuts at: 6591 6676 7747 2442 Size: 85 1071 3225 Fragments arranged by size: 3225 1743 1252 1071 480 183 183 171 123 85 14 BsmI GAATG_Cn' Cuts at: 2518 3072 3800 5302 6041 6134 2518 Size: 554 728 1502 739 93 4914 Fragments arranged by size: 4914 1502 739 728 554 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2442 3694 3708 3771 3783 3891 Size: 399 288 364 1252 14 63 12 108 Cuts at: 3891 4062 4185 4368 4848 5320 5349 5849 6591 Size: 171 123 183 480 472 29 500 742 Cuts at: 6591 6676 7747 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1252 1071 742 500 480 472 399 364 288 183 171 123 108 85 63 29 14 12 BspEI T'CCGG_A Cuts at: 8193 8193 Size: 8530 BspHI T'CATG_A Cuts at: 4075 6419 8149 4075 Size: 2344 1730 4456 Fragments arranged by size: 4456 2344 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4155 5208 5885 844 Size: 3311 1053 677 3489 Fragments arranged by size: 3489 3311 1053 677 BsrBI CCG'CTC Cuts at: 1394 1823 3241 3628 4222 6417 1394 Size: 429 1418 387 594 2195 3507 Fragments arranged by size: 3507 2195 1418 594 429 387 BsrDI GCAATG_nn' Cuts at: 65 1248 2865 7025 7199 65 Size: 1183 1617 4160 174 1396 Fragments arranged by size: 4160 1617 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2423 7174 7361 1700 Size: 110 613 4751 187 2869 Fragments arranged by size: 4751 2869 613 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8530 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8530 BssSI C'ACGA_G Cuts at: 6590 7602 6590 Size: 1012 7518 BstAPI GCAn_nnn'nTGC Cuts at: 794 3337 794 Size: 2543 5987 BstBI TT'CG_AA Cuts at: 7334 7334 Size: 8530 BstEII G'GTnAC_C Cuts at: 1184 2603 3058 3554 1184 Size: 1419 455 496 6160 Fragments arranged by size: 6160 1419 496 455 BstYI r'GATC_y Cuts at: 1972 2528 2976 3169 3333 4511 5860 6227 6612 Size: 556 448 193 164 1178 1349 367 385 Cuts at: 6612 6629 8081 8167 8179 8525 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1349 1178 556 448 385 367 346 193 164 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3260 5811 3260 Size: 2551 5979 BtgI C'CryG_G Cuts at: 513 1065 2004 2352 3933 5445 513 Size: 552 939 348 1581 1512 3598 Fragments arranged by size: 3598 1581 1512 939 552 348 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7791 Fragments arranged by size: 7791 683 56 BtsI GCAGTG_nn' Cuts at: 2440 2464 3577 3638 4062 5818 6046 6840 6860 Size: 24 1113 61 424 1756 228 794 20 Cuts at: 6860 2440 Size: 4110 Fragments arranged by size: 4110 1756 1113 794 424 228 61 24 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4305 4340 529 Size: 35 3741 35 4719 Fragments arranged by size: 4719 3741 35 35 DraI TTT'AAA Cuts at: 2206 2746 3435 4464 4587 5259 5834 2206 Size: 540 689 1029 123 672 575 4902 Fragments arranged by size: 4902 1029 689 672 575 540 123 DraIII CAC_nnn'GTG Cuts at: 2525 2525 Size: 8530 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7537 809 Size: 507 6221 1802 Fragments arranged by size: 6221 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3090 3231 5977 Size: 54 1425 50 275 18 1260 141 2746 Cuts at: 5977 6866 8495 8 Size: 889 1629 43 Fragments arranged by size: 2746 1629 1425 1260 889 275 141 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5977 1537 Size: 275 4165 4090 Fragments arranged by size: 4165 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2550 2946 3270 3393 3588 4254 6460 1353 Size: 1197 396 324 123 195 666 2206 3423 Fragments arranged by size: 3423 2206 1197 666 396 324 195 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3259 4856 7117 7491 7637 387 Size: 1064 138 1670 1597 2261 374 146 1280 Fragments arranged by size: 2261 1670 1597 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3889 4183 4366 4846 3889 Size: 294 183 480 7573 Fragments arranged by size: 7573 480 294 183 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2388 3002 3500 3650 3810 3905 Size: 393 518 238 614 498 150 160 95 Cuts at: 3905 4025 4223 4376 4856 4872 4931 5276 5423 Size: 120 198 153 480 16 59 345 147 Cuts at: 5423 6593 7190 7977 8245 1239 Size: 1170 597 787 268 1524 Fragments arranged by size: 1524 1170 787 614 597 518 498 480 393 345 268 238 198 160 153 150 147 120 95 59 16 EcoNI CCTnn'n_nnAGG Cuts at: 2090 3341 2090 Size: 1251 7279 EcoO109I rG'GnC_Cy Cuts at: 2082 2328 5317 2082 Size: 246 2989 5295 Fragments arranged by size: 5295 2989 246 EcoRI G'AATT_C Cuts at: 5650 5839 6217 5650 Size: 189 378 7963 Fragments arranged by size: 7963 378 189 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4142 4142 Size: 8530 FspI TGC'GCA Cuts at: 7036 7036 Size: 8530 HaeII r_GCGC'y Cuts at: 1678 4251 7677 1678 Size: 2573 3426 2531 Fragments arranged by size: 3426 2573 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3318 3350 3873 3905 4189 Size: 32 135 32 1746 32 523 32 284 Cuts at: 4189 4221 5317 5349 7171 7203 7245 7277 1373 Size: 32 1096 32 1822 32 42 32 2626 Fragments arranged by size: 2626 1822 1746 1096 523 284 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5874 6120 669 Size: 988 281 3936 246 3079 Fragments arranged by size: 3936 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4124 4213 4418 748 Size: 3376 89 205 4860 Fragments arranged by size: 4860 3376 205 89 HpaI GTT'AAC Cuts at: 6120 6120 Size: 8530 KpnI G_GTAC'C Cuts at: 5553 5855 5553 Size: 302 8228 MfeI C'AATT_G Cuts at: 4625 5498 6129 4625 Size: 873 631 7026 Fragments arranged by size: 7026 873 631 MluI A'CGCG_T Cuts at: 8406 8406 Size: 8530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3102 5364 5731 7644 7828 1305 Size: 1797 2262 367 1913 184 2007 Fragments arranged by size: 2262 2007 1913 1797 367 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3092 10 Size: 54 1425 343 1260 5448 Fragments arranged by size: 5448 1425 1260 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4823 5121 6488 6847 7006 518 Size: 648 3657 298 1367 359 159 2042 Fragments arranged by size: 3657 2042 1367 648 359 298 159 MspA1I CmG'CkG Cuts at: 1606 3756 3922 3942 4312 4927 5143 5413 5946 Size: 2150 166 20 370 615 216 270 533 Cuts at: 5946 6623 7771 8016 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2150 2120 1148 677 615 533 370 270 245 216 166 20 NcoI C'CATG_G Cuts at: 513 1065 2004 5445 513 Size: 552 939 3441 3598 Fragments arranged by size: 3598 3441 939 552 NdeI CA'TA_TG Cuts at: 387 3869 387 Size: 3482 5048 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8530 NotI GC'GGCC_GC Cuts at: 5977 5977 Size: 8530 NsiI A_TGCA'T Cuts at: 2920 2920 Size: 8530 NspI r_CATG'y Cuts at: 1132 1229 2122 5888 7433 1132 Size: 97 893 3766 1545 2229 Fragments arranged by size: 3766 2229 1545 893 97 PasI CC'CwG_GG Cuts at: 1348 2086 1348 Size: 738 7792 PciI A'CATG_T Cuts at: 2118 7429 2118 Size: 5311 3219 PflMI CCAn_nnn'nTGG Cuts at: 1108 4627 5446 1108 Size: 3519 819 4192 Fragments arranged by size: 4192 3519 819 PfoI T'CCnGG_A Cuts at: 3411 5210 3411 Size: 1799 6731 PmeI GTTT'AAAC Cuts at: 5834 5834 Size: 8530 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3765 3797 6599 6631 8112 8144 3765 Size: 32 2802 32 1481 32 4151 Fragments arranged by size: 4151 2802 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2082 2328 2082 Size: 246 8284 PshAI GACnn'nnGTC Cuts at: 1613 2326 1613 Size: 713 7817 PsiI TTA'TAA Cuts at: 6100 6100 Size: 8530 PspOMI G'GGCC_C Cuts at: 5316 5316 Size: 8530 PspXI vC'TCGA_Gb Cuts at: 2675 2675 Size: 8530 PstI C_TGCA'G Cuts at: 830 2154 2435 3342 4152 4564 5882 830 Size: 1324 281 907 810 412 1318 3478 Fragments arranged by size: 3478 1324 1318 907 810 412 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8530 PvuII CAG'CTG Cuts at: 3922 3942 4312 4927 5143 5413 3922 Size: 20 370 615 216 270 7039 Fragments arranged by size: 7039 615 370 270 216 20 SacI G_AGCT'C Cuts at: 3891 4185 4368 4848 3891 Size: 294 183 480 7573 Fragments arranged by size: 7573 480 294 183 SalI G'TCGA_C Cuts at: 1936 5872 1936 Size: 3936 4594 SanDI GG'GwC_CC Cuts at: 2082 2082 Size: 8530 SbfI CC_TGCA'GG Cuts at: 4152 5882 4152 Size: 1730 6800 ScaI AGT'ACT Cuts at: 6778 8490 6778 Size: 1712 6818 SfcI C'TryA_G Cuts at: 826 1045 2150 2431 3338 4148 4560 5878 7013 Size: 219 1105 281 907 810 412 1318 1135 Cuts at: 7013 7694 7885 826 Size: 681 191 1471 Fragments arranged by size: 1471 1318 1135 1105 907 810 681 412 281 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8530 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8530 SmaI CCC'GGG Cuts at: 1872 5483 5857 1872 Size: 3611 374 4545 Fragments arranged by size: 4545 3611 374 SmlI C'TyrA_G Cuts at: 820 1017 1943 2042 2675 3191 4061 4127 4184 Size: 197 926 99 633 516 870 66 57 Cuts at: 4184 4206 4340 6634 7535 7797 820 Size: 22 134 2294 901 262 1553 Fragments arranged by size: 2294 1553 926 901 870 633 516 262 197 134 99 66 57 22 SnaBI TAC'GTA Cuts at: 493 6321 493 Size: 5828 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8530 SphI G_CATG'C Cuts at: 1132 5888 1132 Size: 4756 3774 SspI AAT'ATT Cuts at: 5 52 6454 5 Size: 47 6402 2081 Fragments arranged by size: 6402 2081 47 StuI AGG'CCT Cuts at: 1517 2987 4236 5697 1517 Size: 1470 1249 1461 4350 Fragments arranged by size: 4350 1470 1461 1249 StyI C'CwwG_G Cuts at: 513 1065 2004 2988 5445 5969 513 Size: 552 939 984 2457 524 3074 Fragments arranged by size: 3074 2457 984 939 552 524 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6557 6574 6727 6912 7357 325 Size: 1255 4977 17 153 185 445 1498 Fragments arranged by size: 4977 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3252 3279 6776 Size: 274 80 33 51 1443 1275 27 3497 Cuts at: 6776 8488 96 Size: 1712 138 Fragments arranged by size: 3497 1712 1443 1275 274 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2581 2613 5799 5831 8490 8522 2581 Size: 32 3186 32 2659 32 2589 Fragments arranged by size: 3186 2659 2589 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8530 XbaI T'CTAG_A Cuts at: 3466 5866 3466 Size: 2400 6130 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 8530 XhoI C'TCGA_G Cuts at: 1943 2675 1943 Size: 732 7798 XmaI C'CCGG_G Cuts at: 1870 5481 5855 1870 Size: 3611 374 4545 Fragments arranged by size: 4545 3611 374 XmnI GAAnn'nnTTC Cuts at: 2256 4236 4419 5163 5676 6659 2256 Size: 1980 183 744 513 983 4127 Fragments arranged by size: 4127 1980 983 744 513 183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6338 276 Size: 53 83 186 936 4804 2468 Fragments arranged by size: 4804 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI