Restriction map for
pFN21AB6454
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HindIII KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 199 CCGC CCGC(-3/-1)
AclI 433 AACGTT AA^CGTT
AcyI 325 GGCGTC G[GA]^CG[CT]C
AlwI 258 GATCC GGATC(4/5)
AlwNI 1019 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 544 ATTAAT AT^TAAT
AsnI 544 ATTAAT AT^TAAT
AspI 457 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1032 GGACC G^G[AT]CC
BanII 263 GGGCTC G[GA]GC[CT]^C
BbsI 618 GAAGAC GAAGAC(2/6)
BcgI 40 GCATCCCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 515 CTAG C^TAG
BlpI 755 GCTTAGC GC^T[ACGT]AGC
BmyI 263 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 755 GCTTAGC GC^T[ACGT]AGC
BpuAI 618 GAAGAC GAAGAC(2/6)
BsaHI 325 GGCGTC G[GA]^CG[CT]C
Bsp1286I 263 GGGCTC G[AGT]GC[ACT]^C
BspHI 882 TCATGA T^CATGA
BspMI 576 GCAGGT ACCTGC(4/8)
CelII 755 GCTTAGC GC^T[ACGT]AGC
DraII 1031 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 135 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1031 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 427 GAATTC G^AATTC
HaeII 248 AGCGCC [GA]GCGC^[CT]
HgaI 326 GCGTC GACGC(5/10)
Hin1I 325 GGCGTC G[GA]^CG[CT]C
HpaI 436 GTTAAC GTT^AAC
MaeI 515 CTAG C^TAG
MaeII 434 ACGT A^CGT
PflFI 457 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 447 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1031 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 544 ATTAAT AT^TAAT
Psp1406I 433 AACGTT AA^CGTT
RcaI 882 TCATGA T^CATGA
SmlI 788 CTCAAG C^T[CT][GA]AG
SspI 1006 AATATT AAT^ATT
Tth111I 457 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 447 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 427 GAATTC [GA]^AATT[CT]
626 AAATTC
AfaI 362 GTAC GT^AC
557 GTAC
AluI 357 AGCT AG^CT
390 AGCT
ApoI 427 GAATTC [GA]^AATT[CT]
626 AAATTC
BcnI 261 CCGGG CC^[GC]GG
596 CCCGG
BseRI 293 CTCCTC GAGGAG(10/8)
154 GAGGAG
DpnII 258 GATC ^GATC
718 GATC
EarI 118 CTCTTC CTCTTC(1/4)
1037 CTCTTC
EcoT14I 762 CCTTGG C^C[AT][AT]GG
906 CCAAGG
HaeIII 371 GGCC GG^CC
804 GGCC
HapII 261 CCGG C^CGG
597 CCGG
HincII 328 GTCAAC GT[CT]^[GA]AC
436 GTTAAC
HindII 328 GTCAAC GT[CT]^[GA]AC
436 GTTAAC
HpaII 261 CCGG C^CGG
597 CCGG
HphI 255 GGTGA GGTGA(8/7)
868 GGTGA
Ksp632I 118 CTCTTC CTCTTC(1/4)
1037 CTCTTC
MaeIII 499 GTCAC ^GT[ACGT]AC
633 GTCAC
MboI 258 GATC ^GATC
718 GATC
MseI 437 TTAA T^TAA
545 TTAA
MspI 261 CCGG C^CGG
597 CCGG
MwoI 304 GCCTATGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
805 GCCCTGTGTGC
NciI 261 CCGGG CC^[GC]GG
596 CCCGG
NdeII 258 GATC ^GATC
718 GATC
PleI 278 GAGTC GAGTC(4/5)
784 GAGTC
PstI 470 CTGCAG CTGCA^G
835 CTGCAG
RsaI 362 GTAC GT^AC
557 GTAC
Sau3AI 258 GATC ^GATC
718 GATC
StyI 762 CCTTGG C^C[AT][AT]GG
906 CCAAGG
TaqI 720 TCGA T^CGA
969 TCGA
TfiI 691 GAATC G^A[AT]TC
982 GATTC
Tru9I 437 TTAA T^TAA
545 TTAA
Tsp45I 499 GTCAC ^GT[GC]AC
633 GTCAC
TthHB8I 720 TCGA T^CGA
969 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
BbvI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI CfoI Cfr13I DdeI EcoRII
Fnu4HI FokI HhaI HinP1I HinfI
ItaI MboII MnlI MvaI NlaIII
NlaIV Sau96I ScrFI SfaNI SfcI
TseI Tsp509I TspRI