(Circular) (Six-base) MAPSORT of: pFN21AB6454.seq Check: 7775 from: 1 to: 5755 pFN21AB6454 5755 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2572 2572 Size: 5755 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 2439 3882 4255 1563 Size: 876 1443 373 3063 Fragments arranged by size: 3063 1443 876 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3029 3818 5202 5470 1632 Size: 1397 789 1384 268 1917 Fragments arranged by size: 1917 1397 1384 789 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2855 1184 Size: 1671 4084 AlwNI CAG_nnn'CTG Cuts at: 3029 3498 5070 3029 Size: 469 1572 3714 Fragments arranged by size: 3714 1572 469 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 2432 2631 3064 3290 3442 5551 1493 Size: 939 199 433 226 152 2109 1697 Fragments arranged by size: 2109 1697 939 433 226 199 152 AseI AT'TA_AT Cuts at: 160 1032 2550 4309 160 Size: 872 1518 1759 1606 Fragments arranged by size: 1759 1606 1518 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 3080 1870 Size: 73 1137 4545 Fragments arranged by size: 4545 1137 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3076 4531 618 Size: 325 217 627 1289 1455 1842 Fragments arranged by size: 1842 1455 1289 627 325 217 BanII G_rGCy'C Cuts at: 1391 2272 3074 1391 Size: 881 802 4072 Fragments arranged by size: 4072 881 802 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2630 928 Size: 971 731 4053 Fragments arranged by size: 4053 971 731 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2034 2068 3946 3980 1903 Size: 34 97 34 1878 34 3678 Fragments arranged by size: 3678 1878 97 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 136 Size: 107 122 71 631 605 2694 1525 Fragments arranged by size: 2694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 2761 3172 2761 Size: 411 5344 Bme1580I G_kGCm'C Cuts at: 1790 3816 4972 1790 Size: 2026 1156 2573 Fragments arranged by size: 2573 2026 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2356 2663 4415 1239 Size: 1055 62 307 1752 2579 Fragments arranged by size: 2579 1752 1055 307 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2778 3880 4745 5043 2778 Size: 1102 865 298 3490 Fragments arranged by size: 3490 1102 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2331 3562 3944 4579 Size: 53 83 186 936 798 1231 382 635 Cuts at: 4579 275 Size: 1451 Fragments arranged by size: 1451 1231 936 798 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5530 Fragments arranged by size: 5530 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2289 1186 Size: 988 115 4652 Fragments arranged by size: 4652 988 115 BseYI C'CCAG_C Cuts at: 4958 5454 4958 Size: 496 5259 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3816 3901 4972 3816 Size: 85 1071 4599 Fragments arranged by size: 4599 1071 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2272 3074 3816 3901 4972 1391 Size: 399 482 802 742 85 1071 2174 Fragments arranged by size: 2174 1071 802 742 482 399 85 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 2887 3644 5374 2887 Size: 757 1730 3268 Fragments arranged by size: 3268 1730 757 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2572 3110 844 Size: 1728 538 3489 Fragments arranged by size: 3489 1728 538 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4399 4586 1700 Size: 110 2589 187 2869 Fragments arranged by size: 2869 2589 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstYI r'GATC_y Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750 Size: 1113 367 385 17 1452 86 12 346 Cuts at: 5750 1972 Size: 1977 Fragments arranged by size: 1977 1452 1113 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4264 Fragments arranged by size: 4264 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 2484 3271 4065 4085 2484 Size: 787 794 20 4154 Fragments arranged by size: 4154 794 787 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720 Size: 54 1425 50 275 18 1372 889 1629 Cuts at: 5720 8 Size: 43 Fragments arranged by size: 1629 1425 1372 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 3048 3685 1353 Size: 776 919 637 3423 Fragments arranged by size: 3423 919 776 637 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2193 4342 4716 4862 387 Size: 1064 138 604 2149 374 146 1280 Fragments arranged by size: 2149 1280 1064 604 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2356 2663 3029 3818 4415 5202 Size: 393 662 62 307 366 789 597 787 Cuts at: 5202 5470 1239 Size: 268 1524 Fragments arranged by size: 1524 789 787 662 597 393 366 307 268 62 EcoNI CCTnn'n_nnAGG Cuts at: 2144 2144 Size: 5755 EcoO109I rG'GnC_Cy Cuts at: 3037 3037 Size: 5755 EcoRI G'AATT_C Cuts at: 2432 3064 3442 2432 Size: 632 378 4745 Fragments arranged by size: 4745 632 378 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 2257 4902 1678 Size: 579 2645 2531 Fragments arranged by size: 2645 2531 579 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2254 2274 2286 Size: 32 135 32 458 32 192 20 12 Cuts at: 2286 2306 2869 2901 4396 4428 4470 4502 1373 Size: 20 563 32 1495 32 42 32 2626 Fragments arranged by size: 2626 1495 563 458 192 135 42 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2335 2443 3099 3345 669 Size: 988 281 397 108 656 246 3079 Fragments arranged by size: 3079 988 656 397 281 246 108 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 2443 3345 2443 Size: 902 4853 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2059 2239 4869 5053 1305 Size: 754 180 2630 184 2007 Fragments arranged by size: 2630 2007 754 184 180 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3933 Fragments arranged by size: 3933 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3713 4072 4231 518 Size: 648 2547 359 159 2042 Fragments arranged by size: 2547 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3171 3848 4996 5241 1606 Size: 1565 677 1148 245 2120 Fragments arranged by size: 2120 1565 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4264 Fragments arranged by size: 4264 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 3113 4658 1132 Size: 97 1884 1545 2229 Fragments arranged by size: 2229 1884 1545 97 PasI CC'CwG_GG Cuts at: 1348 2326 1348 Size: 978 4777 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 2458 1108 Size: 1350 4405 PfoI T'CCnGG_A Cuts at: 2600 2600 Size: 5755 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3037 3037 Size: 5755 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PstI C_TGCA'G Cuts at: 830 2479 2844 3107 830 Size: 1649 365 263 3478 Fragments arranged by size: 3478 1649 365 263 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SanDI GG'GwC_CC Cuts at: 3037 3037 Size: 5755 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2409 2475 2840 3103 4238 4919 5110 Size: 219 1364 66 365 263 1135 681 191 Cuts at: 5110 826 Size: 1471 Fragments arranged by size: 1471 1364 1135 681 365 263 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2793 3859 4760 5022 820 Size: 197 926 850 1066 901 262 1553 Fragments arranged by size: 1553 1066 926 901 850 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3013 3679 5 Size: 47 2961 666 2081 Fragments arranged by size: 2961 2081 666 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2004 2767 2911 3194 513 Size: 552 939 763 144 283 3074 Fragments arranged by size: 3074 939 763 552 283 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2465 3782 3799 3952 4137 4582 325 Size: 1255 885 1317 17 153 185 445 1498 Fragments arranged by size: 1498 1317 1255 885 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2561 4001 5713 Size: 274 80 33 51 1443 584 1440 1712 Cuts at: 5713 96 Size: 138 Fragments arranged by size: 1712 1443 1440 584 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2073 2105 5715 5747 2073 Size: 32 3610 32 2081 Fragments arranged by size: 3610 2081 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2465 1316 Size: 1149 4606 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI