################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MfeI MluI MluNI MroI MscI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AciI 208 CCGC CCGC(-3/-1) AcyI 175 GACGCC G[GA]^CG[CT]C AflIII 239 ACGTGT A^C[GA][CT]GT AlwI 345 GATCC GGATC(4/5) AlwNI 259 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 69 GGACC G^G[AT]CC BbsI 220 GAAGAC GAAGAC(2/6) BglI 115 GCCAATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 232 GTGCCC G[AGT]GC[ACT]^C BpuAI 220 GAAGAC GAAGAC(2/6) BsaAI 238 TACGTG [CT]AC^GT[GA] BsaBI 13 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 175 GACGCC G[GA]^CG[CT]C BsaI 282 GGTCTC GGTCTC(1/5) BsgI 199 CTGCAC GTGCAG(16/14) BsmAI 283 GTCTC GTCTC(1/5) Bsp1286I 232 GTGCCC G[AGT]GC[ACT]^C BspMI 203 ACCTGC ACCTGC(4/8) BstYI 344 AGATCC [GA]^GATC[CT] CfoI 249 GCGC GCG^C DraII 260 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 260 AGGCCCT [GA]G^G[ACGT]CC[CT] HgaI 175 GACGC GACGC(5/10) HhaI 249 GCGC GCG^C Hin1I 175 GACGCC G[GA]^CG[CT]C HinP1I 249 GCGC G^CGC HinfI 18 GAATC G^A[ACGT]TC MaeII 239 ACGT A^CGT MamI 13 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 220 GAAGA GAAGA(8/7) MflI 344 AGATCC [GA]^GATC[CT] MslI 159 CATCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 86 CATG CATG^ NlaIV 112 GGAGCC GG[ACGT]^[ACGT]CC PstI 170 CTGCAG CTGCA^G PvuII 331 CAGCTG CAG^CTG SfcI 170 CTGCAG C^T[GA][CT]AG TfiI 18 GAATC G^A[AT]TC Tsp45I 310 GTCAC ^GT[GC]AC TspRI 185 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 344 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BpmI 285 CTCCAG CTGGAG(16/14) 187 CTGGAG BsaJI 263 CCCTGG C^C[ACGT][ACGT]GG 264 CCTGGG BsrI 66 ACTGG ACTGG(1/-1) 186 ACTGG BssKI 264 CCTGG ^CC[ACGT]GG 287 CCAGG BstNI 264 CCTGG CC^[AT]GG 287 CCAGG Cac8I 258 GCAGGC GC[ACGT]^[ACGT]GC 326 GCTCGC Cfr13I 69 GGACC G^G[ACGT]CC 261 GGCCC DpnII 192 GATC ^GATC 345 GATC EcoRII 264 CCTGG ^CC[AT]GG 287 CCAGG HphI 53 TCACC GGTGA(8/7) 311 TCACC MaeIII 89 GTAAC ^GT[ACGT]AC 310 GTCAC MboI 192 GATC ^GATC 345 GATC MspA1I 208 CCGCTG C[AC]G^C[GT]G 331 CAGCTG MvaI 264 CCTGG CC^[AT]GG 287 CCAGG NdeII 192 GATC ^GATC 345 GATC Sau3AI 192 GATC ^GATC 345 GATC Sau96I 69 GGACC G^G[ACGT]CC 261 GGCCC ScrFI 264 CCTGG CC^[ACGT]GG 287 CCAGG SfaNI 43 GATGC GCATC(5/9) 146 GCATC StuI 26 AGGCCT AGG^CCT 289 AGGCCT XcmI 56 CCAAGAAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 254 CCAAGCAGGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AluI BbvI BsiYI BslI DdeI Fnu4HI HaeIII ItaI MnlI MwoI TseI