(Circular) (Six-base) MAPSORT of: pFN21AB8977.seq Check: 5337 from: 1 to: 5824 pFN21AB8977 5824 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3167 1937 Size: 1230 4594 Acc65I G'GTAC_C Cuts at: 2772 3145 2772 Size: 373 5451 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2043 3066 3887 5271 5539 1632 Size: 411 1023 821 1384 268 1917 Fragments arranged by size: 1917 1384 1023 821 411 268 AfeI AGC'GCT Cuts at: 2799 2799 Size: 5824 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5824 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3084 1184 Size: 1900 3924 AlwNI CAG_nnn'CTG Cuts at: 2122 2225 2498 2600 2636 3567 5139 2122 Size: 103 273 102 36 931 1572 2807 Fragments arranged by size: 2807 1572 931 273 103 102 36 ApaI G_GGCC'C Cuts at: 2938 2938 Size: 5824 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 3096 3149 1870 Size: 73 1153 53 4545 Fragments arranged by size: 4545 1153 73 53 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2036 2069 2036 Size: 33 5791 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2772 3145 4600 618 Size: 325 217 627 985 373 1455 1842 Fragments arranged by size: 1842 1455 985 627 373 325 217 BanII G_rGCy'C Cuts at: 1391 2154 2259 2625 2938 3143 1391 Size: 763 105 366 313 205 4072 Fragments arranged by size: 4072 763 366 313 205 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2430 928 Size: 971 531 4322 Fragments arranged by size: 4322 971 531 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4015 4049 1903 Size: 34 2078 34 3678 Fragments arranged by size: 3678 2078 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2343 2404 4435 Size: 107 122 71 631 605 671 61 2031 Cuts at: 4435 136 Size: 1525 Fragments arranged by size: 2031 1525 671 631 605 122 107 71 61 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 2646 3241 2646 Size: 595 5229 Bme1580I G_kGCm'C Cuts at: 1790 2938 3096 3885 5041 1790 Size: 1148 158 789 1156 2573 Fragments arranged by size: 2573 1156 1148 789 158 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2528 3555 4513 Size: 416 53 83 399 24 1111 1027 958 Cuts at: 4513 442 Size: 1753 Fragments arranged by size: 1753 1111 1027 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2798 1056 Size: 1742 4082 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2644 2644 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2504 2906 2918 2981 4484 1239 Size: 1265 402 12 63 1503 2579 Fragments arranged by size: 2579 1503 1265 402 63 12 Bpu10I CC'TnA_GC Cuts at: 2374 2412 2374 Size: 38 5786 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2498 3949 4814 5112 2498 Size: 1451 865 298 3210 Fragments arranged by size: 3210 1451 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2597 4487 882 Size: 617 1098 1890 2219 Fragments arranged by size: 2219 1890 1098 617 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4013 4648 275 Size: 53 83 186 936 2098 382 635 1451 Fragments arranged by size: 2098 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2952 4257 4655 4929 5076 5487 2952 Size: 1305 398 274 147 411 3289 Fragments arranged by size: 3289 1305 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2084 2114 1178 Size: 30 165 30 681 30 4888 Fragments arranged by size: 4888 681 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2617 1186 Size: 1431 4393 BseYI C'CCAG_C Cuts at: 2022 2161 2260 5027 5523 2022 Size: 139 99 2767 496 2323 Fragments arranged by size: 2767 2323 496 139 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2640 2900 1163 Size: 573 904 260 4087 Fragments arranged by size: 4087 904 573 260 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2259 3885 3970 5041 2259 Size: 1626 85 1071 3042 Fragments arranged by size: 3042 1626 1071 85 BsmI GAATG_Cn' Cuts at: 2131 3335 3428 2131 Size: 1204 93 4527 Fragments arranged by size: 4527 1204 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2259 2625 2938 3096 3143 3885 Size: 399 364 105 366 313 158 47 742 Cuts at: 3885 3970 5041 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 364 313 158 105 85 47 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3179 844 Size: 2335 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2362 2890 2902 2923 3711 1394 Size: 429 539 528 12 21 788 3507 Fragments arranged by size: 3507 788 539 528 429 21 12 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2740 4468 4655 1700 Size: 110 930 1728 187 2869 Fragments arranged by size: 2869 1728 930 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5824 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstXI CCAn_nnnn'nTGG Cuts at: 2569 2569 Size: 5824 BstYI r'GATC_y Cuts at: 1972 2284 3154 3521 3906 3923 5375 5461 5473 Size: 312 870 367 385 17 1452 86 12 Cuts at: 5473 5819 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 870 385 367 346 312 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2629 2629 Size: 5824 BtgI C'CryG_G Cuts at: 513 1065 2938 513 Size: 552 1873 3399 Fragments arranged by size: 3399 1873 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2478 487 Size: 683 56 1252 3833 Fragments arranged by size: 3833 1252 683 56 BtsI GCAGTG_nn' Cuts at: 2652 3340 4134 4154 2652 Size: 688 794 20 4322 Fragments arranged by size: 4322 794 688 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2729 4831 809 Size: 507 1413 2102 1802 Fragments arranged by size: 2102 1802 1413 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2310 2697 3271 Size: 54 1425 50 275 18 480 387 574 Cuts at: 3271 4160 5789 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 574 480 387 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3042 3754 1353 Size: 1689 712 3423 Fragments arranged by size: 3423 1689 712 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2257 2257 Size: 5824 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2504 2906 2918 2981 3066 3887 Size: 393 411 461 402 12 63 85 821 Cuts at: 3887 4484 5271 5539 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 821 787 597 461 411 402 393 268 85 63 12 EcoO109I rG'GnC_Cy Cuts at: 2633 2934 2633 Size: 301 5523 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2801 4971 1678 Size: 1123 2170 2531 Fragments arranged by size: 2531 2170 1123 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2417 2436 2449 Size: 32 135 32 512 32 301 19 13 Cuts at: 2449 2456 2468 2488 4465 4497 4539 4571 1373 Size: 7 12 20 1977 32 42 32 2626 Fragments arranged by size: 2626 1977 512 301 135 42 32 32 32 32 32 20 19 13 12 7 HincII GTy'rAC Cuts at: 669 1657 1938 2734 3168 3414 669 Size: 988 281 796 434 246 3079 Fragments arranged by size: 3079 988 796 434 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 2776 3149 2776 Size: 373 5451 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2460 2604 2839 4938 5122 1305 Size: 1155 144 235 2099 184 2007 Fragments arranged by size: 2099 2007 1155 235 184 144 MscI TGG'CCA Cuts at: 10 64 1489 1832 2312 2699 10 Size: 54 1425 343 480 387 3135 Fragments arranged by size: 3135 1425 480 387 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3782 4141 4300 518 Size: 648 2616 359 159 2042 Fragments arranged by size: 2616 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2597 2779 2884 2940 2980 3240 3917 Size: 444 547 182 105 56 40 260 677 Cuts at: 3917 5065 5310 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 547 444 260 245 182 105 56 40 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5272 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NheI G'CTAG_C Cuts at: 1052 2794 1052 Size: 1742 4082 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 3182 4727 1132 Size: 97 1953 1545 2229 Fragments arranged by size: 2229 1953 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5824 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 2174 2225 1108 Size: 1066 51 4707 Fragments arranged by size: 4707 1066 51 PfoI T'CCnGG_A Cuts at: 2382 2805 2382 Size: 423 5401 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2934 2934 Size: 5824 PstI C_TGCA'G Cuts at: 830 2118 3176 830 Size: 1288 1058 3478 Fragments arranged by size: 3478 1288 1058 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2050 2597 2884 2980 2050 Size: 547 287 96 4894 Fragments arranged by size: 4894 547 287 96 SacI G_AGCT'C Cuts at: 2259 2259 Size: 5824 SacII CC_GC'GG Cuts at: 2941 2941 Size: 5824 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2114 2458 3172 4307 4988 5179 826 Size: 219 1069 344 714 1135 681 191 1471 Fragments arranged by size: 1471 1135 1069 714 681 344 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 3098 3151 1872 Size: 1226 53 4545 Fragments arranged by size: 4545 1226 53 SmlI C'TyrA_G Cuts at: 820 1017 1943 2477 3928 4829 5091 820 Size: 197 926 534 1451 901 262 1553 Fragments arranged by size: 1553 1451 926 901 534 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 2346 1517 Size: 829 4995 StyI C'CwwG_G Cuts at: 513 1065 2173 2568 2610 2694 3263 513 Size: 552 1108 395 42 84 569 3074 Fragments arranged by size: 3074 1108 569 552 395 84 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2203 2232 3851 3868 4021 4206 4651 Size: 1255 623 29 1619 17 153 185 445 Cuts at: 4651 325 Size: 1498 Fragments arranged by size: 1619 1498 1255 623 445 185 153 29 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4070 5782 96 Size: 274 80 33 51 1443 2093 1712 138 Fragments arranged by size: 2093 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5784 5816 5784 Size: 32 5792 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2222 2881 2222 Size: 659 5165 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 3096 3149 1870 Size: 1226 53 4545 Fragments arranged by size: 4545 1226 53 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI