(Circular) (Six-base) MAPSORT of: pFN21AB6479.seq Check: 2982 from: 1 to: 5686 pFN21AB6479 5686 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2534 3029 1937 Size: 597 495 4594 Fragments arranged by size: 4594 597 495 Acc65I G'GTAC_C Cuts at: 3007 3007 Size: 5686 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2105 2190 3749 5133 5401 1632 Size: 473 85 1559 1384 268 1917 Fragments arranged by size: 1917 1559 1384 473 268 85 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 4585 5562 1203 Size: 3382 977 1327 Fragments arranged by size: 3382 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 2530 4415 2530 Size: 1885 3801 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5686 AlwNI CAG_nnn'CTG Cuts at: 3429 5001 3429 Size: 1572 4114 ApaLI G'TGCA_C Cuts at: 2484 3743 4899 2484 Size: 1259 1156 3271 Fragments arranged by size: 3271 1259 1156 ApoI r'AATT_y Cuts at: 1493 2051 2777 2939 2953 2995 3221 3373 5482 Size: 558 726 162 14 42 226 152 2109 Cuts at: 5482 1493 Size: 1697 Fragments arranged by size: 2109 1697 726 558 226 162 152 42 14 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 3011 1870 Size: 73 1068 4545 Fragments arranged by size: 4545 1068 73 BamHI G'GATC_C Cuts at: 3016 3383 3016 Size: 367 5319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2515 3007 4462 618 Size: 325 217 627 728 492 1455 1842 Fragments arranged by size: 1842 1455 728 627 492 325 217 BanII G_rGCy'C Cuts at: 1391 2510 3005 1391 Size: 1119 495 4072 Fragments arranged by size: 4072 1119 495 BbeI G_GCGC'C Cuts at: 2519 2519 Size: 5686 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2111 2171 928 Size: 971 212 60 4443 Fragments arranged by size: 4443 971 212 60 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3877 3911 1903 Size: 34 1940 34 3678 Fragments arranged by size: 3678 1940 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2088 3578 4788 2088 Size: 1490 1210 2986 Fragments arranged by size: 2986 1490 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5686 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4297 136 Size: 107 122 71 631 605 2625 1525 Fragments arranged by size: 2625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2838 5681 2838 Size: 2843 2843 BlpI GC'TnA_GC Cuts at: 3103 3103 Size: 5686 Bme1580I G_kGCm'C Cuts at: 1790 2488 3747 4903 1790 Size: 698 1259 1156 2573 Fragments arranged by size: 2573 1259 1156 698 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5686 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2608 3417 4375 Size: 416 53 83 399 24 1191 809 958 Cuts at: 4375 442 Size: 1753 Fragments arranged by size: 1753 1191 958 809 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2912 2943 4346 1239 Size: 1673 31 1403 2579 Fragments arranged by size: 2579 1673 1403 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 4676 4974 3811 Size: 865 298 4523 Fragments arranged by size: 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4349 882 Size: 617 2850 2219 Fragments arranged by size: 2850 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2642 3477 493 Size: 711 1438 835 2702 Fragments arranged by size: 2702 1438 835 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2516 3493 3875 4510 Size: 53 83 186 936 983 977 382 635 Cuts at: 4510 275 Size: 1451 Fragments arranged by size: 1451 983 977 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2326 4119 4517 4791 4938 5349 2326 Size: 1793 398 274 147 411 2663 Fragments arranged by size: 2663 1793 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5461 Fragments arranged by size: 5461 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2143 2243 1186 Size: 957 100 4629 Fragments arranged by size: 4629 957 100 BseYI C'CCAG_C Cuts at: 4889 5385 4889 Size: 496 5190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5113 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3897 4925 1540 Size: 275 186 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2488 2510 3747 3832 4903 2488 Size: 22 1237 85 1071 3271 Fragments arranged by size: 3271 1237 1071 85 22 BsmI GAATG_Cn' Cuts at: 3197 3290 3197 Size: 93 5593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2488 2510 3005 3747 3832 4903 1391 Size: 399 698 22 495 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 698 495 399 85 22 BspEI T'CCGG_A Cuts at: 5349 5349 Size: 5686 BspHI T'CATG_A Cuts at: 3575 5305 3575 Size: 1730 3956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3041 844 Size: 2197 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3573 1394 Size: 429 1750 3507 Fragments arranged by size: 3507 1750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2562 4181 4355 65 Size: 1183 1314 1619 174 1396 Fragments arranged by size: 1619 1396 1314 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4330 4517 1700 Size: 110 2520 187 2869 Fragments arranged by size: 2869 2520 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5686 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5686 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5686 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5686 BstYI r'GATC_y Cuts at: 1972 2838 3016 3383 3768 3785 5237 5323 5335 Size: 866 178 367 385 17 1452 86 12 Cuts at: 5335 5681 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 866 385 367 346 178 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2520 2520 Size: 5686 BtgI C'CryG_G Cuts at: 513 1065 2004 2734 513 Size: 552 939 730 3465 Fragments arranged by size: 3465 939 730 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4947 Fragments arranged by size: 4947 683 56 BtsI GCAGTG_nn' Cuts at: 2486 3202 3996 4016 2486 Size: 716 794 20 4156 Fragments arranged by size: 4156 794 716 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2983 2990 2983 Size: 7 5679 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4693 809 Size: 507 3377 1802 Fragments arranged by size: 3377 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3133 4022 5651 Size: 54 1425 50 275 18 1303 889 1629 Cuts at: 5651 8 Size: 43 Fragments arranged by size: 1629 1425 1303 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2848 3616 1353 Size: 1495 768 3423 Fragments arranged by size: 3423 1495 768 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4273 4647 4793 387 Size: 1064 138 2684 374 146 1280 Fragments arranged by size: 2684 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2508 2508 Size: 5686 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2190 2912 2943 3749 4346 5133 Size: 393 473 85 722 31 806 597 787 Cuts at: 5133 5401 1239 Size: 268 1524 Fragments arranged by size: 1524 806 787 722 597 473 393 268 85 31 EcoNI CCTnn'n_nnAGG Cuts at: 2158 2158 Size: 5686 EcoO109I rG'GnC_Cy Cuts at: 2741 2741 Size: 5686 EcoRI G'AATT_C Cuts at: 2995 3373 2995 Size: 378 5308 EcoRV GAT'ATC Cuts at: 2562 2562 Size: 5686 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2598 2598 Size: 5686 FspI TGC'GCA Cuts at: 4192 4192 Size: 5686 HaeII r_GCGC'y Cuts at: 1678 2519 4833 1678 Size: 841 2314 2531 Fragments arranged by size: 2531 2314 841 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2516 2548 2689 2721 2787 Size: 32 135 32 944 32 141 32 66 Cuts at: 2787 2819 2825 2857 4327 4359 4401 4433 1373 Size: 32 6 32 1470 32 42 32 2626 Fragments arranged by size: 2626 1470 944 141 135 66 42 32 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3276 669 Size: 988 281 1092 246 3079 Fragments arranged by size: 3079 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KasI G'GCGC_C Cuts at: 2515 2515 Size: 5686 KpnI G_GTAC'C Cuts at: 3011 3011 Size: 5686 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4800 4984 1305 Size: 3495 184 2007 Fragments arranged by size: 3495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3864 Fragments arranged by size: 3864 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3644 4003 4162 518 Size: 648 2478 359 159 2042 Fragments arranged by size: 2478 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2736 3102 3779 4927 5172 1606 Size: 1130 366 677 1148 245 2120 Fragments arranged by size: 2120 1148 1130 677 366 245 NarI GG'CG_CC Cuts at: 2516 2516 Size: 5686 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4195 Fragments arranged by size: 4195 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5686 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 3044 4589 1132 Size: 97 1815 1545 2229 Fragments arranged by size: 2229 1815 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5686 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5686 PfoI T'CCnGG_A Cuts at: 2564 2805 2889 2564 Size: 241 84 5361 Fragments arranged by size: 5361 241 84 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2741 2741 Size: 5686 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5686 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PstI C_TGCA'G Cuts at: 830 3038 830 Size: 2208 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 SacI G_AGCT'C Cuts at: 2510 2510 Size: 5686 SacII CC_GC'GG Cuts at: 2737 2737 Size: 5686 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SanDI GG'GwC_CC Cuts at: 2741 2741 Size: 5686 SbfI CC_TGCA'GG Cuts at: 3038 3038 Size: 5686 ScaI AGT'ACT Cuts at: 3934 5646 3934 Size: 1712 3974 SfcI C'TryA_G Cuts at: 826 1045 2847 3034 4169 4850 5041 826 Size: 219 1802 187 1135 681 191 1471 Fragments arranged by size: 1802 1471 1135 681 219 191 187 SfoI GGC'GCC Cuts at: 2517 2517 Size: 5686 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 3013 1872 Size: 1141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3790 4691 4953 820 Size: 197 926 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 3044 1132 Size: 1912 3774 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5686 StyI C'CwwG_G Cuts at: 513 1065 2004 2367 2573 2657 2859 2972 3125 Size: 552 939 363 206 84 202 113 153 Cuts at: 3125 513 Size: 3074 Fragments arranged by size: 3074 939 552 363 206 202 153 113 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3713 3730 3883 4068 4513 325 Size: 1255 2133 17 153 185 445 1498 Fragments arranged by size: 2133 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3932 5644 96 Size: 274 80 33 51 1443 1955 1712 138 Fragments arranged by size: 1955 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5646 5678 5646 Size: 32 5654 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 3011 1870 Size: 1141 4545 XmnI GAAnn'nnTTC Cuts at: 3815 3815 Size: 5686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SapI SexAI SfiI SrfI SwaI XcmI