(Circular) (Six-base) MAPSORT of: pFN21AB6155.seq Check: 6244 from: 1 to: 6496 pFN21AB6155 6496 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3681 4306 278 Size: 53 83 186 936 2145 625 2468 Fragments arranged by size: 2468 2145 936 625 186 83 53 AccI GT'mk_AC Cuts at: 1937 3839 1937 Size: 1902 4594 Acc65I G'GTAC_C Cuts at: 3817 3817 Size: 6496 AclI AA'CG_TT Cuts at: 1563 4623 4996 1563 Size: 3060 373 3063 Fragments arranged by size: 3063 3060 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4559 5943 6211 1632 Size: 2927 1384 268 1917 Fragments arranged by size: 2927 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6299 AflIII A'CryG_T Cuts at: 1203 5395 6372 1203 Size: 4192 977 1327 Fragments arranged by size: 4192 1327 977 AgeI A'CCGG_T Cuts at: 5327 5327 Size: 6496 AhdI GACnn_n'nnGTC Cuts at: 5225 5225 Size: 6496 AleI CACnn'nnGTG Cuts at: 1166 3359 1166 Size: 2193 4303 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3111 1184 Size: 1927 4569 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2237 2269 2237 Size: 32 6464 AlwNI CAG_nnn'CTG Cuts at: 2023 2122 2800 3508 4239 5811 2023 Size: 99 678 708 731 1572 2708 Fragments arranged by size: 2708 1572 731 708 678 99 ApaI G_GGCC'C Cuts at: 2418 2418 Size: 6496 ApaLI G'TGCA_C Cuts at: 4553 5709 4553 Size: 1156 5340 ApoI r'AATT_y Cuts at: 1493 3805 4031 4183 6292 1493 Size: 2312 226 152 2109 1697 Fragments arranged by size: 2312 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5050 160 Size: 872 4018 1606 Fragments arranged by size: 4018 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 AvaI C'yCGr_G Cuts at: 1870 1943 2294 3549 3821 1870 Size: 73 351 1255 272 4545 Fragments arranged by size: 4545 1255 351 272 73 BamHI G'GATC_C Cuts at: 3826 4193 3826 Size: 367 6129 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 2554 2892 3304 3817 Size: 325 217 627 304 463 338 412 513 Cuts at: 3817 5272 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 513 463 412 338 325 304 217 BanII G_rGCy'C Cuts at: 1391 2015 2160 2418 2570 2610 2717 3030 3243 Size: 624 145 258 152 40 107 313 213 Cuts at: 3243 3815 1391 Size: 572 4072 Fragments arranged by size: 4072 624 572 313 258 213 152 145 107 40 BbeI G_GCGC'C Cuts at: 2095 2095 Size: 6496 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5525 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4687 4721 1903 Size: 34 50 34 2666 34 3678 Fragments arranged by size: 3678 2666 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4388 5598 4388 Size: 1210 5286 BclI T'GATC_A Cuts at: 1548 3363 1548 Size: 1815 4681 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2046 5107 136 Size: 107 122 71 631 605 374 3061 1525 Fragments arranged by size: 3061 1525 631 605 374 122 107 71 BglII A'GATC_T Cuts at: 6491 6491 Size: 6496 BlpI GC'TnA_GC Cuts at: 3913 3913 Size: 6496 Bme1580I G_kGCm'C Cuts at: 1790 2418 3309 4557 5713 1790 Size: 628 891 1248 1156 2573 Fragments arranged by size: 2573 1248 1156 891 628 BmgBI CAC'GTC Cuts at: 1318 3789 1318 Size: 2471 4025 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2084 3393 3496 Size: 416 53 83 399 24 667 1309 103 Cuts at: 3496 4227 5185 442 Size: 731 958 1753 Fragments arranged by size: 1753 1309 958 731 667 416 399 103 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6496 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2245 2999 3017 3086 3530 3743 5156 1239 Size: 1006 754 18 69 444 213 1413 2579 Fragments arranged by size: 2579 1413 1006 754 444 213 69 18 Bpu10I CC'TnA_GC Cuts at: 2055 2055 Size: 6496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3218 4621 5486 5784 3218 Size: 1403 865 298 3930 Fragments arranged by size: 3930 1403 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2190 5159 882 Size: 617 691 2969 2219 Fragments arranged by size: 2969 2219 691 617 BsaAI yAC'GTr Cuts at: 493 1204 4287 493 Size: 711 3083 2702 Fragments arranged by size: 3083 2702 711 BsaBI GATnn'nnATC Cuts at: 3698 6490 3698 Size: 2792 3704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2092 3678 4303 4685 Size: 53 83 186 936 559 1586 625 382 Cuts at: 4685 5320 275 Size: 635 1451 Fragments arranged by size: 1586 1451 936 635 625 559 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2238 4929 5327 5601 5748 6159 2238 Size: 2691 398 274 147 411 2575 Fragments arranged by size: 2691 2575 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2237 2267 2597 2627 3482 Size: 30 165 30 834 30 330 30 855 Cuts at: 3482 3512 3584 3614 1178 Size: 30 72 30 4060 Fragments arranged by size: 4060 855 834 330 165 72 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2293 2845 3038 3157 3250 3725 1186 Size: 1107 552 193 119 93 475 3957 Fragments arranged by size: 3957 1107 552 475 193 119 93 BseYI C'CCAG_C Cuts at: 2410 2558 2800 2874 3595 5699 6195 2410 Size: 148 242 74 721 2104 496 2711 Fragments arranged by size: 2711 2104 721 496 242 148 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 2324 3236 3596 1163 Size: 573 399 189 912 360 4063 Fragments arranged by size: 4063 912 573 399 360 189 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3146 3946 4707 5735 1540 Size: 275 186 1145 800 761 1028 2301 Fragments arranged by size: 2301 1145 1028 800 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2031 3030 3075 4557 4642 5713 2031 Size: 999 45 1482 85 1071 2814 Fragments arranged by size: 2814 1482 1071 999 85 45 BsmI GAATG_Cn' Cuts at: 4007 4100 4007 Size: 93 6403 BsmBI CGTCTCn'nnnn_ Cuts at: 3685 3779 3685 Size: 94 6402 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2031 2160 2418 2570 2610 2717 Size: 399 225 16 129 258 152 40 107 Cuts at: 2717 3030 3075 3243 3309 3815 4557 4642 5713 Size: 313 45 168 66 506 742 85 1071 Cuts at: 5713 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 506 399 313 258 225 168 152 129 107 85 66 45 40 16 BspEI T'CCGG_A Cuts at: 2238 6159 2238 Size: 3921 2575 BspHI T'CATG_A Cuts at: 4385 6115 4385 Size: 1730 4766 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2256 3851 844 Size: 1412 1595 3489 Fragments arranged by size: 3489 1595 1412 BsrBI CCG'CTC Cuts at: 1394 1823 2937 2983 4383 1394 Size: 429 1114 46 1400 3507 Fragments arranged by size: 3507 1400 1114 429 46 BsrDI GCAATG_nn' Cuts at: 65 1248 4991 5165 65 Size: 1183 3743 174 1396 Fragments arranged by size: 3743 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2094 2663 3045 3582 5140 5327 1700 Size: 110 284 569 382 537 1558 187 2869 Fragments arranged by size: 2869 1558 569 537 382 284 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6496 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6496 BssSI C'ACGA_G Cuts at: 2863 2897 3203 4556 5568 2863 Size: 34 306 1353 1012 3791 Fragments arranged by size: 3791 1353 1012 306 34 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6496 BstBI TT'CG_AA Cuts at: 2946 5300 2946 Size: 2354 4142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6496 BstYI r'GATC_y Cuts at: 1972 2499 3198 3826 4193 4578 4595 6047 6133 Size: 527 699 628 367 385 17 1452 86 Cuts at: 6133 6145 6491 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 699 628 527 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2837 513 Size: 552 1772 4172 Fragments arranged by size: 4172 1772 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2409 2902 3145 487 Size: 683 56 1183 493 243 3838 Fragments arranged by size: 3838 1183 683 493 243 56 BtsI GCAGTG_nn' Cuts at: 2256 4012 4806 4826 2256 Size: 1756 794 20 3926 Fragments arranged by size: 3926 1756 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6496 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6461 DraI TTT'AAA Cuts at: 3800 3800 Size: 6496 DraIII CAC_nnn'GTG Cuts at: 2899 2899 Size: 6496 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2282 5503 809 Size: 507 966 3221 1802 Fragments arranged by size: 3221 1802 966 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 3143 3943 Size: 54 1425 50 275 18 498 815 800 Cuts at: 3943 4832 6461 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 815 800 498 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3143 3943 1537 Size: 275 1331 800 4090 Fragments arranged by size: 4090 1331 800 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 2828 4426 1353 Size: 683 792 1598 3423 Fragments arranged by size: 3423 1598 792 683 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5083 5457 5603 387 Size: 1064 138 3494 374 146 1280 Fragments arranged by size: 3494 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3028 3028 Size: 6496 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2245 2999 3017 3086 3530 3743 4559 Size: 393 613 754 18 69 444 213 816 Cuts at: 4559 5156 5943 6211 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 816 787 754 613 597 444 393 268 213 69 18 EcoNI CCTnn'n_nnAGG Cuts at: 2549 3288 2549 Size: 739 5757 EcoO109I rG'GnC_Cy Cuts at: 2119 2414 3150 3250 3293 3374 2119 Size: 295 736 100 43 81 5241 Fragments arranged by size: 5241 736 295 100 81 43 EcoRI G'AATT_C Cuts at: 3805 4183 3805 Size: 378 6118 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2338 2338 Size: 6496 FspI TGC'GCA Cuts at: 5002 5002 Size: 6496 HaeII r_GCGC'y Cuts at: 1678 2095 2373 3775 5643 1678 Size: 417 278 1402 1868 2531 Fragments arranged by size: 2531 1868 1402 417 278 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2062 2094 2597 2629 2840 Size: 32 135 32 490 32 503 32 211 Cuts at: 2840 2872 3393 3425 3584 3616 5137 5169 5211 Size: 32 521 32 159 32 1521 32 42 Cuts at: 5211 5243 1373 Size: 32 2626 Fragments arranged by size: 2626 1521 521 503 490 211 159 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3840 4086 669 Size: 988 281 1902 246 3079 Fragments arranged by size: 3079 1902 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6496 HpaI GTT'AAC Cuts at: 4086 4086 Size: 6496 KasI G'GCGC_C Cuts at: 2091 2091 Size: 6496 KpnI G_GTAC'C Cuts at: 3821 3821 Size: 6496 MfeI C'AATT_G Cuts at: 4095 4095 Size: 6496 MluI A'CGCG_T Cuts at: 6372 6372 Size: 6496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2046 2673 2787 3102 5610 5794 1305 Size: 741 627 114 315 2508 184 2007 Fragments arranged by size: 2508 2007 741 627 315 184 114 MscI TGG'CCA Cuts at: 10 64 1489 1832 2330 10 Size: 54 1425 343 498 4176 Fragments arranged by size: 4176 1425 498 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3359 4454 4813 4972 518 Size: 648 2193 1095 359 159 2042 Fragments arranged by size: 2193 2042 1095 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2581 2785 2839 3095 3465 3674 3912 4589 Size: 975 204 54 256 370 209 238 677 Cuts at: 4589 5737 5982 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 975 677 370 256 245 238 209 204 54 NaeI GCC'GGC Cuts at: 2096 2096 Size: 6496 NarI GG'CG_CC Cuts at: 2092 2092 Size: 6496 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5944 NdeI CA'TA_TG Cuts at: 387 387 Size: 6496 NgoMIV G'CCGG_C Cuts at: 2094 2094 Size: 6496 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6496 NotI GC'GGCC_GC Cuts at: 3943 3943 Size: 6496 NruI TCG'CGA Cuts at: 2082 2082 Size: 6496 NspI r_CATG'y Cuts at: 1132 1229 3854 5399 1132 Size: 97 2625 1545 2229 Fragments arranged by size: 2625 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3284 3427 1348 Size: 1936 143 4417 Fragments arranged by size: 4417 1936 143 PciI A'CATG_T Cuts at: 5395 5395 Size: 6496 PflMI CCAn_nnn'nTGG Cuts at: 1108 2800 2911 2977 1108 Size: 1692 111 66 4627 Fragments arranged by size: 4627 1692 111 66 PfoI T'CCnGG_A Cuts at: 2506 2679 3063 3411 3588 3705 2506 Size: 173 384 348 177 117 5297 Fragments arranged by size: 5297 384 348 177 173 117 PmeI GTTT'AAAC Cuts at: 3800 3800 Size: 6496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2237 2269 4565 4597 6078 6110 2237 Size: 32 2296 32 1481 32 2623 Fragments arranged by size: 2623 2296 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3293 3293 Size: 6496 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6496 PsiI TTA'TAA Cuts at: 4066 4066 Size: 6496 PspOMI G'GGCC_C Cuts at: 2414 2414 Size: 6496 PstI C_TGCA'G Cuts at: 830 2358 2835 3198 3728 3848 830 Size: 1528 477 363 530 120 3478 Fragments arranged by size: 3478 1528 530 477 363 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6496 PvuII CAG'CTG Cuts at: 3095 3095 Size: 6496 SacI G_AGCT'C Cuts at: 3030 3030 Size: 6496 SacII CC_GC'GG Cuts at: 2840 2840 Size: 6496 SalI G'TCGA_C Cuts at: 1936 3838 1936 Size: 1902 4594 SapI GCTCTTCn'nnn_ Cuts at: 2036 2828 2036 Size: 792 5704 SbfI CC_TGCA'GG Cuts at: 3848 3848 Size: 6496 ScaI AGT'ACT Cuts at: 4744 6456 4744 Size: 1712 4784 SfcI C'TryA_G Cuts at: 826 1045 2184 2354 2831 3194 3210 3724 3844 Size: 219 1139 170 477 363 16 514 120 Cuts at: 3844 4979 5660 5851 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1139 1135 681 514 477 363 219 191 170 120 16 SfoI GGC'GCC Cuts at: 2093 2093 Size: 6496 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 SgrAI Cr'CCGG_yG Cuts at: 1700 2094 1700 Size: 394 6102 SmaI CCC'GGG Cuts at: 1872 3551 3823 1872 Size: 1679 272 4545 Fragments arranged by size: 4545 1679 272 SmlI C'TyrA_G Cuts at: 820 1017 1943 3233 4600 5501 5763 820 Size: 197 926 1290 1367 901 262 1553 Fragments arranged by size: 1553 1367 1290 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4287 493 Size: 3794 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6496 SphI G_CATG'C Cuts at: 1132 3854 1132 Size: 2722 3774 SspI AAT'ATT Cuts at: 5 52 4420 5 Size: 47 4368 2081 Fragments arranged by size: 4368 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6496 StyI C'CwwG_G Cuts at: 513 1065 2191 3013 3935 513 Size: 552 1126 822 922 3074 Fragments arranged by size: 3074 1126 922 822 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2572 2653 3304 4523 4540 4693 4878 Size: 1255 992 81 651 1219 17 153 185 Cuts at: 4878 5323 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1219 992 651 445 185 153 81 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4742 6454 96 Size: 274 80 33 51 1443 2765 1712 138 Fragments arranged by size: 2765 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2885 2917 3482 3514 3759 3791 6456 6488 2885 Size: 32 565 32 245 32 2665 32 2893 Fragments arranged by size: 2893 2665 565 245 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2076 1316 Size: 760 5736 XbaI T'CTAG_A Cuts at: 3832 3832 Size: 6496 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3434 3713 3434 Size: 279 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6496 XmaI C'CCGG_G Cuts at: 1870 3549 3821 1870 Size: 1679 272 4545 Fragments arranged by size: 4545 1679 272 XmnI GAAnn'nnTTC Cuts at: 2219 2627 4625 2219 Size: 408 1998 4090 Fragments arranged by size: 4090 1998 408 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3679 4304 276 Size: 53 83 186 936 2145 625 2468 Fragments arranged by size: 2468 2145 936 625 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI