Restriction map for
pFN21AB6155
(Linear) (Six-base) MAPSORT of: KIBB6155.seq Check: 3644 from: 1 to: 1791
KIBB6155.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AatII G_ACGT'C
Cuts at: 0 1676 1791
Size: 1676 115
AleI CACnn'nnGTG
Cuts at: 0 1354 1791
Size: 1354 437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1106 1791
Size: 1106 685
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 232 264 1791
Size: 232 32 1527
Fragments arranged by size:
1527 232 32
AlwNI CAG_nnn'CTG
Cuts at: 0 18 117 795 1503 1791
Size: 18 99 678 708 288
Fragments arranged by size:
708 678 288 99 18
ApaI G_GGCC'C
Cuts at: 0 413 1791
Size: 413 1378
AvaI C'yCGr_G
Cuts at: 0 289 1544 1791
Size: 289 1255 247
Fragments arranged by size:
1255 289 247
BanI G'GyrC_C
Cuts at: 0 86 549 887 1299 1791
Size: 86 463 338 412 492
Fragments arranged by size:
492 463 412 338 86
BanII G_rGCy'C
Cuts at: 0 10 155 413 565 605 712 1025 1238
Size: 10 145 258 152 40 107 313 213
Cuts at: 1238 1791
Size: 553
Fragments arranged by size:
553 313 258 213 152 145 107 40
10
BbeI G_GCGC'C
Cuts at: 0 90 1791
Size: 90 1701
BclI T'GATC_A
Cuts at: 0 1358 1791
Size: 1358 433
BglI GCCn_nnn'nGGC
Cuts at: 0 41 1791
Size: 41 1750
Bme1580I G_kGCm'C
Cuts at: 0 413 1304 1791
Size: 413 891 487
Fragments arranged by size:
891 487 413
BmgBI CAC'GTC
Cuts at: 0 1784 1791
Size: 1784 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 79 1388 1491 1791
Size: 79 1309 103 300
Fragments arranged by size:
1309 300 103 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 994 1012 1081 1525 1738 1791
Size: 240 754 18 69 444 213 53
Fragments arranged by size:
754 444 240 213 69 53 18
Bpu10I CC'TnA_GC
Cuts at: 0 50 1791
Size: 50 1741
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1213 1791
Size: 1213 578
BsaI GGTCTCn'nnnn_
Cuts at: 0 185 1791
Size: 185 1606
BsaBI GATnn'nnATC
Cuts at: 0 1693 1791
Size: 1693 98
BsaHI Gr'CG_yC
Cuts at: 0 87 1673 1791
Size: 87 1586 118
Fragments arranged by size:
1586 118 87
BsaWI w'CCGG_w
Cuts at: 0 233 1791
Size: 233 1558
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 592 622 1477 1507 1579 1609
Size: 232 30 330 30 855 30 72 30
Cuts at: 1609 1791
Size: 182
Fragments arranged by size:
855 330 232 182 72 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 288 840 1033 1152 1245 1720 1791
Size: 288 552 193 119 93 475 71
Fragments arranged by size:
552 475 288 193 119 93 71
BseYI C'CCAG_C
Cuts at: 0 405 553 795 869 1590 1791
Size: 405 148 242 74 721 201
Fragments arranged by size:
721 405 242 201 148 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 319 1231 1591 1791
Size: 130 189 912 360 200
Fragments arranged by size:
912 360 200 189 130
BsiEI CG_ry'CG
Cuts at: 0 1141 1791
Size: 1141 650
BsiHKAI G_wGCw'C
Cuts at: 0 26 1025 1070 1791
Size: 26 999 45 721
Fragments arranged by size:
999 721 45 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1680 1774 1791
Size: 1680 94 17
Fragments arranged by size:
1680 94 17
Bsp1286I G_dGCh'C
Cuts at: 0 10 26 155 413 565 605 712 1025
Size: 10 16 129 258 152 40 107 313
Cuts at: 1025 1070 1238 1304 1791
Size: 45 168 66 487
Fragments arranged by size:
487 313 258 168 152 129 107 66
45 40 16 10
BspEI T'CCGG_A
Cuts at: 0 233 1791
Size: 233 1558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 251 1791
Size: 251 1540
BsrBI CCG'CTC
Cuts at: 0 932 978 1791
Size: 932 46 813
Fragments arranged by size:
932 813 46
BsrFI r'CCGG_y
Cuts at: 0 89 658 1040 1577 1791
Size: 89 569 382 537 214
Fragments arranged by size:
569 537 382 214 89
BssSI C'ACGA_G
Cuts at: 0 858 892 1198 1791
Size: 858 34 306 593
Fragments arranged by size:
858 593 306 34
BstBI TT'CG_AA
Cuts at: 0 941 1791
Size: 941 850
BstYI r'GATC_y
Cuts at: 0 494 1193 1791
Size: 494 699 598
Fragments arranged by size:
699 598 494
BtgI C'CryG_G
Cuts at: 0 832 1791
Size: 832 959
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 404 897 1140 1791
Size: 404 493 243 651
Fragments arranged by size:
651 493 404 243
BtsI GCAGTG_nn'
Cuts at: 0 251 1791
Size: 251 1540
DraIII CAC_nnn'GTG
Cuts at: 0 894 1791
Size: 894 897
DrdI GACnn_nn'nnGTC
Cuts at: 0 277 1791
Size: 277 1514
EaeI y'GGCC_r
Cuts at: 0 323 1138 1791
Size: 323 815 653
Fragments arranged by size:
815 653 323
EagI C'GGCC_G
Cuts at: 0 1138 1791
Size: 1138 653
EarI CTCTTCn'nnn_
Cuts at: 0 31 823 1791
Size: 31 792 968
Fragments arranged by size:
968 792 31
EcoICRI GAG'CTC
Cuts at: 0 1023 1791
Size: 1023 768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 994 1012 1081 1525 1738 1791
Size: 240 754 18 69 444 213 53
Fragments arranged by size:
754 444 240 213 69 53 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 544 1283 1791
Size: 544 739 508
Fragments arranged by size:
739 544 508
EcoO109I rG'GnC_Cy
Cuts at: 0 114 409 1145 1245 1288 1369 1791
Size: 114 295 736 100 43 81 422
Fragments arranged by size:
736 422 295 114 100 81 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 333 1791
Size: 333 1458
HaeII r_GCGC'y
Cuts at: 0 90 368 1770 1791
Size: 90 278 1402 21
Fragments arranged by size:
1402 278 90 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 57 89 592 624 835 867 1388 1420
Size: 57 32 503 32 211 32 521 32
Cuts at: 1420 1579 1611 1791
Size: 159 32 180
Fragments arranged by size:
521 503 211 180 159 57 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 86 1791
Size: 86 1705
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 668 782 1097 1791
Size: 41 627 114 315 694
Fragments arranged by size:
694 627 315 114 41
MscI TGG'CCA
Cuts at: 0 325 1791
Size: 325 1466
MslI CAynn'nnrTG
Cuts at: 0 1354 1791
Size: 1354 437
MspA1I CmG'CkG
Cuts at: 0 576 780 834 1090 1460 1669 1791
Size: 576 204 54 256 370 209 122
Fragments arranged by size:
576 370 256 209 204 122 54
NaeI GCC'GGC
Cuts at: 0 91 1791
Size: 91 1700
NarI GG'CG_CC
Cuts at: 0 87 1791
Size: 87 1704
NgoMIV G'CCGG_C
Cuts at: 0 89 1791
Size: 89 1702
NruI TCG'CGA
Cuts at: 0 77 1791
Size: 77 1714
PasI CC'CwG_GG
Cuts at: 0 1279 1422 1791
Size: 1279 143 369
Fragments arranged by size:
1279 369 143
PflMI CCAn_nnn'nTGG
Cuts at: 0 795 906 972 1791
Size: 795 111 66 819
Fragments arranged by size:
819 795 111 66
PfoI T'CCnGG_A
Cuts at: 0 501 674 1058 1406 1583 1700 1791
Size: 501 173 384 348 177 117 91
Fragments arranged by size:
501 384 348 177 173 117 91
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 1791
Size: 232 32 1527
Fragments arranged by size:
1527 232 32
PpuMI rG'GwC_Cy
Cuts at: 0 1288 1791
Size: 1288 503
PspOMI G'GGCC_C
Cuts at: 0 409 1791
Size: 409 1382
PstI C_TGCA'G
Cuts at: 0 353 830 1193 1723 1791
Size: 353 477 363 530 68
Fragments arranged by size:
530 477 363 353 68
PvuII CAG'CTG
Cuts at: 0 1090 1791
Size: 1090 701
SacI G_AGCT'C
Cuts at: 0 1025 1791
Size: 1025 766
SacII CC_GC'GG
Cuts at: 0 835 1791
Size: 835 956
SapI GCTCTTCn'nnn_
Cuts at: 0 31 823 1791
Size: 31 792 968
Fragments arranged by size:
968 792 31
SfcI C'TryA_G
Cuts at: 0 179 349 826 1189 1205 1719 1791
Size: 179 170 477 363 16 514 72
Fragments arranged by size:
514 477 363 179 170 72 16
SfoI GGC'GCC
Cuts at: 0 88 1791
Size: 88 1703
SgrAI Cr'CCGG_yG
Cuts at: 0 89 1791
Size: 89 1702
SmaI CCC'GGG
Cuts at: 0 1546 1791
Size: 1546 245
SmlI C'TyrA_G
Cuts at: 0 1228 1791
Size: 1228 563
StyI C'CwwG_G
Cuts at: 0 186 1008 1791
Size: 186 822 783
Fragments arranged by size:
822 783 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 567 648 1299 1791
Size: 567 81 651 492
Fragments arranged by size:
651 567 492 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 880 912 1477 1509 1754 1786 1791
Size: 880 32 565 32 245 32 5
Fragments arranged by size:
880 565 245 32 32 32 5
Tth111I GACn'n_nGTC
Cuts at: 0 71 1791
Size: 71 1720
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1429 1708 1791
Size: 1429 279 83
Fragments arranged by size:
1429 279 83
XmaI C'CCGG_G
Cuts at: 0 1544 1791
Size: 1544 247
XmnI GAAnn'nnTTC
Cuts at: 0 214 622 1791
Size: 214 408 1169
Fragments arranged by size:
1169 408 214
ZraI GAC'GTC
Cuts at: 0 1674 1791
Size: 1674 117
Enzymes that do cut:
AatII AleI AlfI AloI AlwNI ApaI AvaI BanI
BanII BbeI BclI BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BssSI BstBI BstYI BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NruI PasI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuII SacI SacII SapI
SfcI SfoI SgrAI SmaI SmlI StyI TaqII TstI
Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BglII
BlpI BmtI BplI BsaAI BsiWI BsmI BspHI BsrDI
BsrGI BssHII BstAPI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraI EciI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NsiI NspI PacI PciI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI XbaI XhoI