Restriction map for
pFN21AB8889
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BseAI BseRI BsgI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DpnII DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII KpnI Ksp632I MaeI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MunI NdeI NdeII
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
Sau3AI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp509I Tth111I XbaI
XhoI XhoII
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 397 GACGTC GACGT^C
AfaI 84 GTAC GT^AC
AgeI 179 ACCGGT A^CCGGT
AlwNI 415 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 216 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 435 GAGCTC G[GA]GC[CT]^C
BbeI 117 GGCGCC GGCGC^C
BlpI 432 GCTGAGC GC^T[ACGT]AGC
BpmI 322 CTGGAG CTGGAG(16/14)
Bpu1102I 432 GCTGAGC GC^T[ACGT]AGC
BsaWI 179 ACCGGT [AT]^CCGG[AT]
BsiEI 155 CGGCCG CG[GA][CT]^CG
BsrBI 58 GAGCGG CCGCTC(-3/-3)
BssSI 298 CTCGTG CACGAG(-5/-1)
BstEII 339 GGTGACC G^GT[ACGT]ACC
BstPI 339 GGTGACC G^GT[ACGT]ACC
CelII 432 GCTGAGC GC^T[ACGT]AGC
DraII 511 AGGACCC [GA]G^G[ACGT]CC[CT]
EagI 155 CGGCCG C^GGCCG
EclXI 155 CGGCCG C^GGCCG
Eco52I 155 CGGCCG C^GGCCG
EcoO109I 511 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 339 GGTGACC G^GT[ACGT]ACC
EcoT14I 29 CCATGG C^C[AT][AT]GG
HpaI 573 GTTAAC GTT^AAC
KasI 117 GGCGCC G^GCGCC
MaeII 398 ACGT A^CGT
MseI 574 TTAA T^TAA
MslI 24 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 114 GCCGGC GCC^GGC
NarI 117 GGCGCC GG^CGCC
NcoI 29 CCATGG C^CATGG
NgoMIV 114 GCCGGC G^CCGGC
NotI 154 GCGGCCGC GC^GGCCGC
PflMI 414 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 179 ACCGGT A^CCGGT
PpuMI 511 AGGACCC [GA]G^G[AT]CC[CT]
PstI 469 CTGCAG CTGCA^G
PvuII 544 CAGCTG CAG^CTG
RsaI 84 GTAC GT^AC
SacI 435 GAGCTC GAGCT^C
SfcI 469 CTGCAG C^T[GA][CT]AG
SfoI 117 GGCGCC GGC^GCC
StyI 29 CCATGG C^C[AT][AT]GG
TaqI 224 TCGA T^CGA
Tru9I 574 TTAA T^TAA
TspRI 524 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 224 TCGA T^CGA
Van91I 414 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 523 CCACTGGCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 216 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 117 GGCGCC G[GA]^CG[CT]C
397 GACGTC
AspHI 253 GTGCTC G[AT]GC[AT]^C
435 GAGCTC
BanI 103 GGCACC G^G[CT][GA]CC
117 GGCGCC
BmyI 253 GTGCTC G[AGT]GC[ACT]^C
435 GAGCTC
BsaHI 117 GGCGCC G[GA]^CG[CT]C
397 GACGTC
BsiHKAI 253 GTGCTC G[AT]GC[AT]^C
435 GAGCTC
BsmFI 313 GGGAC GGGAC(10/14)
407 GGGAC
Bsp1286I 253 GTGCTC G[AGT]GC[ACT]^C
435 GAGCTC
BsrFI 114 GCCGGC [GA]^CCGG[CT]
179 ACCGGT
BsrI 525 ACTGG ACTGG(1/-1)
9 CCAGT
BstF5I 122 CATCC GGATG(2/0)
489 GGATG
Cfr10I 114 GCCGGC [GA]^CCGG[CT]
179 ACCGGT
DdeI 433 CTGAG C^T[ACGT]AG
476 CTCAG
FokI 122 CATCC GGATG(9/13)
489 GGATG
HgaI 294 GACGC GACGC(5/10)
315 GACGC
Hin1I 117 GGCGCC G[GA]^CG[CT]C
397 GACGTC
HincII 4 GTTGAC GT[CT]^[GA]AC
573 GTTAAC
HindII 4 GTTGAC GT[CT]^[GA]AC
573 GTTAAC
HinfI 174 GATTC G^A[ACGT]TC
247 GATTC
KspI 152 CCGCGG CCGC^GG
373 CCGCGG
MboII 354 GAAGA GAAGA(8/7)
506 TCTTC
SacII 152 CCGCGG CCGC^GG
373 CCGCGG
TfiI 174 GATTC G^A[AT]TC
247 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BbvI BcnI BsaJI BsiYI BslI
BsoBI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DsaI EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeIII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SmaI TseI Tsp45I
XmaCI XmaI