Restriction map for
pFN21AB6434
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AspEI AviII BalI BbeI BbrPI
BbsI BclI BfrI BglII BpuAI
BsaAI BsaHI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HpaI KasI KspI
MaeIII MfeI MluI MluNI MroI
MscI MunI NarI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 195 GGTACC G^GTACC
AfaI 196 GTAC GT^AC
Asp718I 195 GGTACC G^GTACC
AspI 760 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 751 CCCGAG C^[CT]CG[GA]G
AvrII 951 CCTAGG C^CTAGG
BanII 807 GGGCTC G[GA]GC[CT]^C
BcgI 728 GCAGACACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 596 GGATAC GTATCC(6/5)
BcnI 762 CCCGG CC^[GC]GG
BglI 582 GCCAGATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 951 CCTAGG C^CTAGG
BlpI 670 GCTCAGC GC^T[ACGT]AGC
BpmI 897 CTCCAG CTGGAG(16/14)
Bpu1102I 670 GCTCAGC GC^T[ACGT]AGC
BsaBI 255 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 680 GAGACC GGTCTC(1/5)
BsaWI 348 ACCGGA [AT]^CCGG[AT]
BsoBI 751 CCCGAG C^[CT]CG[GA]G
BspHI 534 TCATGA T^CATGA
BspMI 632 GCAGGT ACCTGC(4/8)
BsrDI 1005 CATTGC GCAATG(2/0)
BsrFI 325 GCCGGC [GA]^CCGG[CT]
BstAPI 661 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 670 GCTCAGC GC^T[ACGT]AGC
Cfr10I 325 GCCGGC [GA]^CCGG[CT]
DraII 47 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 983 CCATGG C^C[GA][CT]GG
EaeI 2 TGGCCG [CT]^GGCC[GA]
EarI 819 GAAGAG CTCTTC(1/4)
EcoNI 258 CCTGATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 47 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 538 GATATC GAT^ATC
HgaI 244 GACGC GACGC(5/10)
HindIII 207 AAGCTT A^AGCTT
KpnI 195 GGTACC GGTAC^C
Ksp632I 819 GAAGAG CTCTTC(1/4)
MaeII 548 ACGT A^CGT
MamI 255 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 307 TTAA T^TAA
MslI 906 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 199 CCGCTG C[AC]G^C[GT]G
NaeI 325 GCCGGC GCC^GGC
NciI 762 CCCGG CC^[GC]GG
NcoI 983 CCATGG C^CATGG
NdeI 442 CATATG CA^TATG
NgoMIV 325 GCCGGC G^CCGGC
PflFI 760 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 441 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 47 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 390 CTGCAG CTGCA^G
RcaI 534 TCATGA T^CATGA
RsaI 196 GTAC GT^AC
SapI 819 GAAGAGC GCTCTTC(1/4)
TaqI 263 TCGA T^CGA
Tru9I 307 TTAA T^TAA
Tsp509I 657 AATT ^AATT
Tth111I 760 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 263 TCGA T^CGA
Van91I 441 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 6 CGCG CG^CG
246 CGCG
Asp700I 841 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
888 GAACTTCTTC
BamHI 186 GGATCC G^GATCC
254 GGATCC
BbvCI 270 GCTGAGG CCTCAGC(-5/-2)
918 GCTGAGG
BsmAI 680 GAGAC GTCTC(1/5)
58 GTCTC
BsrBI 932 CCGCTC CCGCTC(-3/-3)
225 GAGCGG
BsrI 110 ACTGG ACTGG(1/-1)
470 ACTGG
BstUI 6 CGCG CG^CG
246 CGCG
CfoI 856 GCGC GCG^C
995 GCGC
EcoT14I 951 CCTAGG C^C[AT][AT]GG
983 CCATGG
FauI 692 CCCGC CCCGC(4/6)
247 GCGGG
HaeII 855 GGCGCT [GA]GCGC^[CT]
994 AGCGCC
HhaI 856 GCGC GCG^C
995 GCGC
HinP1I 856 GCGC G^CGC
995 GCGC
HinfI 505 GACTC G^A[ACGT]TC
788 GACTC
MvnI 6 CGCG CG^CG
246 CGCG
PleI 505 GACTC GAGTC(4/5)
788 GACTC
SfaNI 319 GATGC GCATC(5/9)
720 GATGC
SfcI 390 CTGCAG C^T[GA][CT]AG
990 CTATAG
StyI 951 CCTAGG C^C[AT][AT]GG
983 CCATGG
XcmI 774 CCAGGGCAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
794 CCATCCTGTGCGTGG
XmnI 841 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
888 GAACTTCTTC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanI BbvI BfaI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeI MboI MboII
MflI MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TseI TspRI XhoII