(Circular) (Six-base) MAPSORT of: pFN21AB6434.seq Check: 7455 from: 1 to: 5737 pFN21AB6434 5737 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3547 278 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3080 1937 Size: 1143 4594 Acc65I G'GTAC_C Cuts at: 2200 3058 2200 Size: 858 4879 AclI AA'CG_TT Cuts at: 1563 3864 4237 1563 Size: 2301 373 3063 Fragments arranged by size: 3063 2301 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2692 2861 3800 5184 5452 1632 Size: 1060 169 939 1384 268 1917 Fragments arranged by size: 1917 1384 1060 939 268 169 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5540 AflIII A'CryG_T Cuts at: 1203 4636 5613 1203 Size: 3433 977 1327 Fragments arranged by size: 3433 1327 977 AgeI A'CCGG_T Cuts at: 4568 4568 Size: 5737 AhdI GACnn_n'nnGTC Cuts at: 4466 4466 Size: 5737 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5737 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5737 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2885 2917 2885 Size: 32 5705 AlwNI CAG_nnn'CTG Cuts at: 3480 5052 3480 Size: 1572 4165 ApaLI G'TGCA_C Cuts at: 3794 4950 3794 Size: 1156 4581 ApoI r'AATT_y Cuts at: 1493 3046 3272 3424 5533 1493 Size: 1553 226 152 2109 1697 Fragments arranged by size: 2109 1697 1553 226 152 AseI AT'TA_AT Cuts at: 160 1032 4291 160 Size: 872 3259 1606 Fragments arranged by size: 3259 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 AvaI C'yCGr_G Cuts at: 1870 1943 2756 3062 1870 Size: 73 813 306 4545 Fragments arranged by size: 4545 813 306 73 AvrII C'CTAG_G Cuts at: 2956 2956 Size: 5737 BamHI G'GATC_C Cuts at: 2191 2259 3067 3434 2191 Size: 68 808 367 4494 Fragments arranged by size: 4494 808 367 68 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2200 2584 2976 3058 4513 Size: 325 217 627 413 384 392 82 1455 Cuts at: 4513 618 Size: 1842 Fragments arranged by size: 1842 1455 627 413 392 384 325 217 82 BanII G_rGCy'C Cuts at: 1391 2816 3056 1391 Size: 1425 240 4072 Fragments arranged by size: 4072 1425 240 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4766 BbvCI CC'TCA_GC Cuts at: 2276 2924 2276 Size: 648 5089 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2722 2756 3928 3962 1903 Size: 34 785 34 1172 34 3678 Fragments arranged by size: 3678 1172 785 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2595 3629 4839 2595 Size: 1034 1210 3493 Fragments arranged by size: 3493 1210 1034 BclI T'GATC_A Cuts at: 1548 1548 Size: 5737 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2593 4348 136 Size: 107 122 71 631 605 921 1755 1525 Fragments arranged by size: 1755 1525 921 631 605 122 107 71 BglII A'GATC_T Cuts at: 5732 5732 Size: 5737 BlpI GC'TnA_GC Cuts at: 2676 3154 2676 Size: 478 5259 Bme1580I G_kGCm'C Cuts at: 1790 2979 3798 4954 1790 Size: 1189 819 1156 2573 Fragments arranged by size: 2573 1189 1156 819 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5737 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3468 4426 442 Size: 416 53 83 399 24 2051 958 1753 Fragments arranged by size: 2051 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5737 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2387 2387 Size: 5737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2887 4397 1239 Size: 1648 1510 2579 Fragments arranged by size: 2579 1648 1510 Bpu10I CC'TnA_GC Cuts at: 2276 2924 2276 Size: 648 5089 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3862 4727 5025 3862 Size: 865 298 4574 Fragments arranged by size: 4574 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2679 4400 882 Size: 617 1180 1721 2219 Fragments arranged by size: 2219 1721 1180 617 BsaAI yAC'GTr Cuts at: 493 1204 3528 493 Size: 711 2324 2702 Fragments arranged by size: 2702 2324 711 BsaBI GATnn'nnATC Cuts at: 2264 5731 2264 Size: 3467 2270 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3544 3926 4561 275 Size: 53 83 186 936 2011 382 635 1451 Fragments arranged by size: 2011 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2353 4170 4568 4842 4989 5400 2353 Size: 1817 398 274 147 411 2690 Fragments arranged by size: 2690 1817 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2885 2915 1178 Size: 30 165 30 1482 30 4000 Fragments arranged by size: 4000 1482 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2526 2923 2972 1186 Size: 1099 241 397 49 3951 Fragments arranged by size: 3951 1099 397 241 49 BseYI C'CCAG_C Cuts at: 2439 2645 2717 2728 2829 4940 5436 2439 Size: 206 72 11 101 2111 496 2740 Fragments arranged by size: 2740 2111 496 206 101 72 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5164 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3187 3948 4976 1540 Size: 275 186 1186 761 1028 2301 Fragments arranged by size: 2301 1186 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2108 2373 2677 3798 3883 4954 2108 Size: 265 304 1121 85 1071 2891 Fragments arranged by size: 2891 1121 1071 304 265 85 BsmI GAATG_Cn' Cuts at: 3248 3341 3248 Size: 93 5644 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2373 2677 2816 2979 3056 3798 Size: 399 318 265 304 139 163 77 742 Cuts at: 3798 3883 4954 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 318 304 265 163 139 85 77 BspEI T'CCGG_A Cuts at: 5400 5400 Size: 5737 BspHI T'CATG_A Cuts at: 2539 3626 5356 2539 Size: 1087 1730 2920 Fragments arranged by size: 2920 1730 1087 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2628 3092 844 Size: 1784 464 3489 Fragments arranged by size: 3489 1784 464 BsrBI CCG'CTC Cuts at: 1394 1823 2232 2939 3624 1394 Size: 429 409 707 685 3507 Fragments arranged by size: 3507 707 685 429 409 BsrDI GCAATG_nn' Cuts at: 65 1248 3009 4232 4406 65 Size: 1183 1761 1223 174 1396 Fragments arranged by size: 1761 1396 1223 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2330 4381 4568 1700 Size: 110 520 2051 187 2869 Fragments arranged by size: 2869 2051 520 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5737 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5737 BssSI C'ACGA_G Cuts at: 3797 4809 3797 Size: 1012 4725 BstAPI GCAn_nnn'nTGC Cuts at: 794 2672 794 Size: 1878 3859 BstBI TT'CG_AA Cuts at: 4541 4541 Size: 5737 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5737 BstYI r'GATC_y Cuts at: 1972 2079 2191 2259 2880 3067 3434 3819 3836 Size: 107 112 68 621 187 367 385 17 Cuts at: 3836 5288 5374 5386 5732 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 621 385 367 346 187 112 107 86 68 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2988 513 Size: 552 939 984 3262 Fragments arranged by size: 3262 984 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2323 487 Size: 683 56 1097 3901 Fragments arranged by size: 3901 1097 683 56 BtsI GCAGTG_nn' Cuts at: 2499 2754 3253 4047 4067 2499 Size: 255 499 794 20 4169 Fragments arranged by size: 4169 794 499 255 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5737 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5702 DraI TTT'AAA Cuts at: 3041 3041 Size: 5737 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4744 809 Size: 507 3428 1802 Fragments arranged by size: 3428 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3184 4073 Size: 54 1425 50 275 18 177 1177 889 Cuts at: 4073 5702 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1177 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3184 1537 Size: 275 1372 4090 Fragments arranged by size: 4090 1372 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2819 3667 1353 Size: 1466 848 3423 Fragments arranged by size: 3423 1466 848 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4324 4698 4844 387 Size: 1064 138 2735 374 146 1280 Fragments arranged by size: 2735 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2692 2861 2887 3800 4397 5184 5452 Size: 393 1060 169 26 913 597 787 268 Cuts at: 5452 1239 Size: 1524 Fragments arranged by size: 1524 1060 913 787 597 393 268 169 26 EcoNI CCTnn'n_nnAGG Cuts at: 2267 2267 Size: 5737 EcoO109I rG'GnC_Cy Cuts at: 2053 2053 Size: 5737 EcoRI G'AATT_C Cuts at: 3046 3424 3046 Size: 378 5359 EcoRV GAT'ATC Cuts at: 2545 2545 Size: 5737 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2870 2870 Size: 5737 FspI TGC'GCA Cuts at: 4243 4243 Size: 5737 HaeII r_GCGC'y Cuts at: 1678 2864 3003 4884 1678 Size: 1186 139 1881 2531 Fragments arranged by size: 2531 1881 1186 139 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2037 2069 2593 2625 2784 Size: 32 135 32 465 32 524 32 159 Cuts at: 2784 2816 2872 2904 4378 4410 4452 4484 1373 Size: 32 56 32 1474 32 42 32 2626 Fragments arranged by size: 2626 1474 524 465 159 135 56 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3081 3327 669 Size: 988 281 1143 246 3079 Fragments arranged by size: 3079 1143 988 281 246 HindIII A'AGCT_T Cuts at: 748 2212 748 Size: 1464 4273 HpaI GTT'AAC Cuts at: 3327 3327 Size: 5737 KpnI G_GTAC'C Cuts at: 2204 3062 2204 Size: 858 4879 MfeI C'AATT_G Cuts at: 3336 3336 Size: 5737 MluI A'CGCG_T Cuts at: 5613 5613 Size: 5737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2415 4851 5035 1305 Size: 1110 2436 184 2007 Fragments arranged by size: 2436 2007 1110 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3915 Fragments arranged by size: 3915 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2915 3695 4054 4213 518 Size: 648 1749 780 359 159 2042 Fragments arranged by size: 2042 1749 780 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2206 3153 3830 4978 5223 1606 Size: 600 947 677 1148 245 2120 Fragments arranged by size: 2120 1148 947 677 600 245 NaeI GCC'GGC Cuts at: 2332 2332 Size: 5737 NcoI C'CATG_G Cuts at: 513 1065 2004 2988 513 Size: 552 939 984 3262 Fragments arranged by size: 3262 984 939 552 NdeI CA'TA_TG Cuts at: 387 2448 387 Size: 2061 3676 NgoMIV G'CCGG_C Cuts at: 2330 2330 Size: 5737 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5737 NotI GC'GGCC_GC Cuts at: 3184 3184 Size: 5737 NspI r_CATG'y Cuts at: 1132 1229 3095 4640 1132 Size: 97 1866 1545 2229 Fragments arranged by size: 2229 1866 1545 97 PasI CC'CwG_GG Cuts at: 1348 2869 1348 Size: 1521 4216 PciI A'CATG_T Cuts at: 4636 4636 Size: 5737 PflMI CCAn_nnn'nTGG Cuts at: 1108 2452 1108 Size: 1344 4393 PmeI GTTT'AAAC Cuts at: 3041 3041 Size: 5737 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2885 2917 3806 3838 5319 5351 2885 Size: 32 889 32 1481 32 3271 Fragments arranged by size: 3271 1481 889 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2053 2053 Size: 5737 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5737 PsiI TTA'TAA Cuts at: 3307 3307 Size: 5737 PstI C_TGCA'G Cuts at: 830 2399 3089 830 Size: 1569 690 3478 Fragments arranged by size: 3478 1569 690 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5737 SalI G'TCGA_C Cuts at: 1936 3079 1936 Size: 1143 4594 SapI GCTCTTCn'nnn_ Cuts at: 2819 2819 Size: 5737 SbfI CC_TGCA'GG Cuts at: 3089 3089 Size: 5737 ScaI AGT'ACT Cuts at: 3985 5697 3985 Size: 1712 4025 SfcI C'TryA_G Cuts at: 826 1045 2395 2995 3085 4220 4901 5092 826 Size: 219 1350 600 90 1135 681 191 1471 Fragments arranged by size: 1471 1350 1135 681 600 219 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5737 SmaI CCC'GGG Cuts at: 1872 3064 1872 Size: 1192 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3841 4742 5004 820 Size: 197 926 1898 901 262 1553 Fragments arranged by size: 1898 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3528 493 Size: 3035 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5737 SphI G_CATG'C Cuts at: 1132 3095 1132 Size: 1963 3774 SspI AAT'ATT Cuts at: 5 52 3661 5 Size: 47 3609 2081 Fragments arranged by size: 3609 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5737 StyI C'CwwG_G Cuts at: 513 1065 2004 2956 2988 3176 513 Size: 552 939 952 32 188 3074 Fragments arranged by size: 3074 952 939 552 188 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2298 2444 2933 3764 3781 3934 4119 Size: 1255 718 146 489 831 17 153 185 Cuts at: 4119 4564 325 Size: 445 1498 Fragments arranged by size: 1498 1255 831 718 489 445 185 153 146 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3983 5695 96 Size: 274 80 33 51 1443 2006 1712 138 Fragments arranged by size: 2006 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5697 5729 5697 Size: 32 5705 Tth111I GACn'n_nGTC Cuts at: 1316 2768 1316 Size: 1452 4285 XbaI T'CTAG_A Cuts at: 3073 3073 Size: 5737 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2786 2806 2786 Size: 20 5717 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5737 XmaI C'CCGG_G Cuts at: 1870 3062 1870 Size: 1192 4545 XmnI GAAnn'nnTTC Cuts at: 2850 2897 3866 2850 Size: 47 969 4721 Fragments arranged by size: 4721 969 47 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3545 276 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI