Restriction map for
pFN21AB6434
(Linear) (Six-base) MAPSORT of: KIBB6434.seq Check: 7905 from: 1 to: 1032
KIBB6434.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
Acc65I G'GTAC_C
Cuts at: 0 195 1032
Size: 195 837
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 856 1032
Size: 687 169 176
Fragments arranged by size:
687 176 169
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 880 912 1032
Size: 880 32 120
Fragments arranged by size:
880 120 32
AvaI C'yCGr_G
Cuts at: 0 751 1032
Size: 751 281
AvrII C'CTAG_G
Cuts at: 0 951 1032
Size: 951 81
BamHI G'GATC_C
Cuts at: 0 186 254 1032
Size: 186 68 778
Fragments arranged by size:
778 186 68
BanI G'GyrC_C
Cuts at: 0 195 579 971 1032
Size: 195 384 392 61
Fragments arranged by size:
392 384 195 61
BanII G_rGCy'C
Cuts at: 0 811 1032
Size: 811 221
BbvCI CC'TCA_GC
Cuts at: 0 271 919 1032
Size: 271 648 113
Fragments arranged by size:
648 271 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 717 751 1032
Size: 717 34 281
Fragments arranged by size:
717 281 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 590 1032
Size: 590 442
BglI GCCn_nnn'nGGC
Cuts at: 0 588 1032
Size: 588 444
BlpI GC'TnA_GC
Cuts at: 0 671 1032
Size: 671 361
Bme1580I G_kGCm'C
Cuts at: 0 974 1032
Size: 974 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 382 1032
Size: 382 650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 882 1032
Size: 882 150
Bpu10I CC'TnA_GC
Cuts at: 0 271 919 1032
Size: 271 648 113
Fragments arranged by size:
648 271 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 674 1032
Size: 674 358
BsaBI GATnn'nnATC
Cuts at: 0 259 1032
Size: 259 773
BsaWI w'CCGG_w
Cuts at: 0 348 1032
Size: 348 684
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 880 910 1032
Size: 880 30 122
Fragments arranged by size:
880 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 521 918 967 1032
Size: 280 241 397 49 65
Fragments arranged by size:
397 280 241 65 49
BseYI C'CCAG_C
Cuts at: 0 434 640 712 723 824 1032
Size: 434 206 72 11 101 208
Fragments arranged by size:
434 208 206 101 72 11
BsiHKAI G_wGCw'C
Cuts at: 0 103 368 672 1032
Size: 103 265 304 360
Fragments arranged by size:
360 304 265 103
Bsp1286I G_dGCh'C
Cuts at: 0 103 368 672 811 974 1032
Size: 103 265 304 139 163 58
Fragments arranged by size:
304 265 163 139 103 58
BspHI T'CATG_A
Cuts at: 0 534 1032
Size: 534 498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 623 1032
Size: 623 409
BsrBI CCG'CTC
Cuts at: 0 227 934 1032
Size: 227 707 98
Fragments arranged by size:
707 227 98
BsrDI GCAATG_nn'
Cuts at: 0 1004 1032
Size: 1004 28
BsrFI r'CCGG_y
Cuts at: 0 325 1032
Size: 325 707
BstAPI GCAn_nnn'nTGC
Cuts at: 0 667 1032
Size: 667 365
BstYI r'GATC_y
Cuts at: 0 74 186 254 875 1032
Size: 74 112 68 621 157
Fragments arranged by size:
621 157 112 74 68
BtgI C'CryG_G
Cuts at: 0 983 1032
Size: 983 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 318 1032
Size: 318 714
BtsI GCAGTG_nn'
Cuts at: 0 494 749 1032
Size: 494 255 283
Fragments arranged by size:
494 283 255
EaeI y'GGCC_r
Cuts at: 0 2 1032
Size: 2 1030
EarI CTCTTCn'nnn_
Cuts at: 0 814 1032
Size: 814 218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 856 882 1032
Size: 687 169 26 150
Fragments arranged by size:
687 169 150 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 1032
Size: 262 770
EcoO109I rG'GnC_Cy
Cuts at: 0 48 1032
Size: 48 984
EcoRV GAT'ATC
Cuts at: 0 540 1032
Size: 540 492
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 865 1032
Size: 865 167
HaeII r_GCGC'y
Cuts at: 0 859 998 1032
Size: 859 139 34
Fragments arranged by size:
859 139 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 588 620 779 811 867 899
Size: 32 32 524 32 159 32 56 32
Cuts at: 899 1032
Size: 133
Fragments arranged by size:
524 159 133 56 32 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 207 1032
Size: 207 825
KpnI G_GTAC'C
Cuts at: 0 199 1032
Size: 199 833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1032
Size: 410 622
MslI CAynn'nnrTG
Cuts at: 0 910 1032
Size: 910 122
MspA1I CmG'CkG
Cuts at: 0 201 1032
Size: 201 831
NaeI GCC'GGC
Cuts at: 0 327 1032
Size: 327 705
NcoI C'CATG_G
Cuts at: 0 983 1032
Size: 983 49
NdeI CA'TA_TG
Cuts at: 0 443 1032
Size: 443 589
NgoMIV G'CCGG_C
Cuts at: 0 325 1032
Size: 325 707
PasI CC'CwG_GG
Cuts at: 0 864 1032
Size: 864 168
PflMI CCAn_nnn'nTGG
Cuts at: 0 447 1032
Size: 447 585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 880 912 1032
Size: 880 32 120
Fragments arranged by size:
880 120 32
PpuMI rG'GwC_Cy
Cuts at: 0 48 1032
Size: 48 984
PstI C_TGCA'G
Cuts at: 0 394 1032
Size: 394 638
SapI GCTCTTCn'nnn_
Cuts at: 0 814 1032
Size: 814 218
SfcI C'TryA_G
Cuts at: 0 390 990 1032
Size: 390 600 42
Fragments arranged by size:
600 390 42
StyI C'CwwG_G
Cuts at: 0 951 983 1032
Size: 951 32 49
Fragments arranged by size:
951 49 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 293 439 928 1032
Size: 293 146 489 104
Fragments arranged by size:
489 293 146 104
Tth111I GACn'n_nGTC
Cuts at: 0 763 1032
Size: 763 269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 781 801 1032
Size: 781 20 231
Fragments arranged by size:
781 231 20
XmnI GAAnn'nnTTC
Cuts at: 0 845 892 1032
Size: 845 47 140
Fragments arranged by size:
845 140 47
Enzymes that do cut:
Acc65I AcuI AloI AvaI AvrII BamHI BanI BanII
BbvCI BcgI BciVI BglI BlpI Bme1580I BplI BpmI
Bpu10I BsaI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstYI
BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HindIII KpnI MmeI MslI
MspA1I NaeI NcoI NdeI NgoMIV PasI PflMI PpiI
PpuMI PstI SapI SfcI StyI TaqII Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI BaeI BbeI BbsI BclI BfrBI BglII
BmgBI BmrI BmtI BpuEI BsaAI BsaHI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI FseI FspI FspAI
HincII HpaI KasI MfeI MluI MscI NarI NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI XbaI
XhoI XmaI ZraI