(Circular) (Six-base) MAPSORT of: pFN21AE0531.seq Check: 7165 from: 1 to: 5938 pFN21AE0531 5938 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2390 2390 Size: 5938 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3117 3748 278 Size: 53 83 186 936 1581 631 2468 Fragments arranged by size: 2468 1581 936 631 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 3117 3281 1937 Size: 517 663 164 4594 Fragments arranged by size: 4594 663 517 164 Acc65I G'GTAC_C Cuts at: 2508 3198 3259 2508 Size: 690 61 5187 Fragments arranged by size: 5187 690 61 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2033 2366 3010 3165 4001 5385 5653 1632 Size: 401 333 644 155 836 1384 268 1917 Fragments arranged by size: 1917 1384 836 644 401 333 268 155 AfeI AGC'GCT Cuts at: 2294 2294 Size: 5938 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 2035 4837 5814 1203 Size: 832 2802 977 1327 Fragments arranged by size: 2802 1327 977 832 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 4667 4667 Size: 5938 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5938 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5938 AlwNI CAG_nnn'CTG Cuts at: 2050 3681 5253 2050 Size: 1631 1572 2735 Fragments arranged by size: 2735 1631 1572 ApaI G_GGCC'C Cuts at: 2503 2809 2503 Size: 306 5632 ApaLI G'TGCA_C Cuts at: 3995 5151 3995 Size: 1156 4782 ApoI r'AATT_y Cuts at: 1493 3247 3473 3625 5734 1493 Size: 1754 226 152 2109 1697 Fragments arranged by size: 2109 1754 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 2489 2802 3263 1870 Size: 73 546 313 461 4545 Fragments arranged by size: 4545 546 461 313 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2848 2881 2848 Size: 33 5905 BamHI G'GATC_C Cuts at: 2284 2926 3268 3635 2284 Size: 642 342 367 4587 Fragments arranged by size: 4587 642 367 342 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2399 2508 3198 3259 4714 Size: 325 217 627 612 109 690 61 1455 Cuts at: 4714 618 Size: 1842 Fragments arranged by size: 1842 1455 690 627 612 325 217 109 61 BanII G_rGCy'C Cuts at: 1391 2070 2503 2809 3257 1391 Size: 679 433 306 448 4072 Fragments arranged by size: 4072 679 448 433 306 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2021 3013 928 Size: 971 122 992 3853 Fragments arranged by size: 3853 992 971 122 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4129 4163 1903 Size: 34 50 34 2108 34 3678 Fragments arranged by size: 3678 2108 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3216 3830 5040 3216 Size: 614 1210 4114 Fragments arranged by size: 4114 1210 614 BclI T'GATC_A Cuts at: 1548 2910 3168 1548 Size: 1362 258 4318 Fragments arranged by size: 4318 1362 258 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2813 4549 136 Size: 107 122 71 631 605 1141 1736 1525 Fragments arranged by size: 1736 1525 1141 631 605 122 107 71 BglII A'GATC_T Cuts at: 5933 5933 Size: 5938 BlpI GC'TnA_GC Cuts at: 3355 3355 Size: 5938 Bme1580I G_kGCm'C Cuts at: 1790 2503 2745 2809 3999 5155 1790 Size: 713 242 64 1190 1156 2573 Fragments arranged by size: 2573 1190 1156 713 242 64 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5938 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3669 4627 442 Size: 416 53 83 399 24 2252 958 1753 Fragments arranged by size: 2252 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3012 1056 Size: 1956 3982 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3242 4598 1239 Size: 2003 1356 2579 Fragments arranged by size: 2579 2003 1356 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2447 4063 4928 5226 2447 Size: 1616 865 298 3159 Fragments arranged by size: 3159 1616 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2789 4601 882 Size: 617 1290 1812 2219 Fragments arranged by size: 2219 1812 1290 617 BsaAI yAC'GTr Cuts at: 493 1204 3729 493 Size: 711 2525 2702 Fragments arranged by size: 2702 2525 711 BsaBI GATnn'nnATC Cuts at: 2915 5932 2915 Size: 3017 2921 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3114 3745 4127 4762 Size: 53 83 186 936 1581 631 382 635 Cuts at: 4762 275 Size: 1451 Fragments arranged by size: 1581 1451 936 635 631 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2184 4371 4769 5043 5190 5601 2184 Size: 2187 398 274 147 411 2521 Fragments arranged by size: 2521 2187 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2318 2348 3047 3077 1178 Size: 30 165 30 915 30 699 30 4039 Fragments arranged by size: 4039 915 699 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2507 3052 1186 Size: 1321 545 4072 Fragments arranged by size: 4072 1321 545 BseYI C'CCAG_C Cuts at: 2029 3231 5141 5637 2029 Size: 1202 1910 496 2330 Fragments arranged by size: 2330 1910 1202 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5365 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3388 4149 5177 1540 Size: 275 186 1387 761 1028 2301 Fragments arranged by size: 2301 1387 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 2682 2709 3999 4084 5155 2025 Size: 657 27 1290 85 1071 2808 Fragments arranged by size: 2808 1290 1071 657 85 27 BsmI GAATG_Cn' Cuts at: 2307 2539 3449 3542 2307 Size: 232 910 93 4703 Fragments arranged by size: 4703 910 232 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2070 2503 2682 2709 2745 2809 Size: 399 235 45 433 179 27 36 64 Cuts at: 2809 3257 3999 4084 5155 1391 Size: 448 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 448 433 399 235 179 85 64 45 36 27 BspEI T'CCGG_A Cuts at: 5601 5601 Size: 5938 BspHI T'CATG_A Cuts at: 2907 3827 5557 2907 Size: 920 1730 3288 Fragments arranged by size: 3288 1730 920 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2390 2868 3226 3293 844 Size: 1546 478 358 67 3489 Fragments arranged by size: 3489 1546 478 358 67 BsrBI CCG'CTC Cuts at: 1394 1823 2497 3825 1394 Size: 429 674 1328 3507 Fragments arranged by size: 3507 1328 674 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4433 4607 65 Size: 1183 3185 174 1396 Fragments arranged by size: 3185 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2732 4582 4769 1700 Size: 110 922 1850 187 2869 Fragments arranged by size: 2869 1850 922 187 110 BsrGI T'GTAC_A Cuts at: 96 2982 96 Size: 2886 3052 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 3998 5010 3998 Size: 1012 4926 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5938 BstBI TT'CG_AA Cuts at: 4742 4742 Size: 5938 BstEII G'GTnAC_C Cuts at: 1184 2390 1184 Size: 1206 4732 BstYI r'GATC_y Cuts at: 1972 2284 2926 3268 3635 4020 4037 5489 5575 Size: 312 642 342 367 385 17 1452 86 Cuts at: 5575 5587 5933 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 642 385 367 346 342 312 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2932 2932 Size: 5938 BtgI C'CryG_G Cuts at: 513 1065 2085 513 Size: 552 1020 4366 Fragments arranged by size: 4366 1020 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2274 2536 487 Size: 683 56 1048 262 3889 Fragments arranged by size: 3889 1048 683 262 56 BtsI GCAGTG_nn' Cuts at: 3454 4248 4268 3454 Size: 794 20 5124 Fragments arranged by size: 5124 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5903 DraI TTT'AAA Cuts at: 3242 3242 Size: 5938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2417 4945 809 Size: 507 1101 2528 1802 Fragments arranged by size: 2528 1802 1101 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2269 2570 2730 Size: 54 1425 50 275 18 439 301 160 Cuts at: 2730 3070 3385 4274 5903 8 Size: 340 315 889 1629 43 Fragments arranged by size: 1629 1425 889 439 340 315 301 275 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 3868 1353 Size: 818 1697 3423 Fragments arranged by size: 3423 1697 818 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2636 2863 4525 4899 5045 387 Size: 1064 138 1047 227 1662 374 146 1280 Fragments arranged by size: 1662 1280 1064 1047 374 227 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2366 3010 3165 3242 4001 4598 Size: 393 401 333 644 155 77 759 597 Cuts at: 4598 5385 5653 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 759 644 597 401 393 333 268 155 77 EcoO109I rG'GnC_Cy Cuts at: 2137 2137 Size: 5938 EcoRI G'AATT_C Cuts at: 3247 3625 3247 Size: 378 5560 FseI GG_CCGG'CC Cuts at: 2736 2736 Size: 5938 FspI TGC'GCA Cuts at: 4444 4444 Size: 5938 HaeII r_GCGC'y Cuts at: 1678 2296 3015 5085 1678 Size: 618 719 2070 2531 Fragments arranged by size: 2531 2070 719 618 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2239 2269 2271 2301 3104 Size: 32 135 32 667 30 2 30 803 Cuts at: 3104 3136 4579 4611 4653 4685 1373 Size: 32 1443 32 42 32 2626 Fragments arranged by size: 2626 1443 803 667 135 42 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 3282 3528 669 Size: 988 281 1344 246 3079 Fragments arranged by size: 3079 1344 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5938 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KpnI G_GTAC'C Cuts at: 2512 3202 3263 2512 Size: 690 61 5187 Fragments arranged by size: 5187 690 61 MfeI C'AATT_G Cuts at: 3537 3537 Size: 5938 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3024 5052 5236 1305 Size: 1719 2028 184 2007 Fragments arranged by size: 2028 2007 1719 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2572 3072 10 Size: 54 1425 343 740 500 2876 Fragments arranged by size: 2876 1425 740 500 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 2384 2748 2978 3896 4255 4414 Size: 648 1008 210 364 230 918 359 159 Cuts at: 4414 518 Size: 2042 Fragments arranged by size: 2042 1008 918 648 364 359 230 210 159 MspA1I CmG'CkG Cuts at: 1606 2115 2568 3030 3076 3354 4031 5179 5424 Size: 509 453 462 46 278 677 1148 245 Cuts at: 5424 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 509 462 453 278 245 46 NaeI GCC'GGC Cuts at: 2734 2734 Size: 5938 NcoI C'CATG_G Cuts at: 513 1065 2085 513 Size: 552 1020 4366 Fragments arranged by size: 4366 1020 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5938 NgoMIV G'CCGG_C Cuts at: 2732 2732 Size: 5938 NheI G'CTAG_C Cuts at: 1052 3008 1052 Size: 1956 3982 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NruI TCG'CGA Cuts at: 2259 2259 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 2039 2461 2764 2989 3296 4841 1132 Size: 97 810 422 303 225 307 1545 2229 Fragments arranged by size: 2229 1545 810 422 307 303 225 97 PasI CC'CwG_GG Cuts at: 1348 2338 2503 2738 1348 Size: 990 165 235 4548 Fragments arranged by size: 4548 990 235 165 PciI A'CATG_T Cuts at: 2035 4837 2035 Size: 2802 3136 PflMI CCAn_nnn'nTGG Cuts at: 1108 2091 2884 1108 Size: 983 793 4162 Fragments arranged by size: 4162 983 793 PfoI T'CCnGG_A Cuts at: 2421 2658 2421 Size: 237 5701 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4007 4039 5520 5552 4007 Size: 32 1481 32 4393 Fragments arranged by size: 4393 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 2137 Size: 5938 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5938 PsiI TTA'TAA Cuts at: 3508 3508 Size: 5938 PspOMI G'GGCC_C Cuts at: 2499 2805 2499 Size: 306 5632 PstI C_TGCA'G Cuts at: 830 2076 2567 2724 2898 3035 3290 830 Size: 1246 491 157 174 137 255 3478 Fragments arranged by size: 3478 1246 491 255 174 157 137 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 PvuII CAG'CTG Cuts at: 2568 3030 3076 2568 Size: 462 46 5430 Fragments arranged by size: 5430 462 46 SalI G'TCGA_C Cuts at: 1936 3280 1936 Size: 1344 4594 SapI GCTCTTCn'nnn_ Cuts at: 2171 2171 Size: 5938 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 4186 5898 4186 Size: 1712 4226 SfcI C'TryA_G Cuts at: 826 1045 2072 2563 2608 2720 2894 3031 3286 Size: 219 1027 491 45 112 174 137 255 Cuts at: 3286 4421 5102 5293 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1027 681 491 255 219 191 174 137 112 45 SfiI GGCCn_nnn'nGGCC Cuts at: 2813 2813 Size: 5938 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 2804 3265 1872 Size: 932 461 4545 Fragments arranged by size: 4545 932 461 SmlI C'TyrA_G Cuts at: 820 1017 1943 2462 4042 4943 5205 820 Size: 197 926 519 1580 901 262 1553 Fragments arranged by size: 1580 1553 926 901 519 262 197 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 3296 1132 Size: 2164 3774 SspI AAT'ATT Cuts at: 5 52 3862 5 Size: 47 3810 2081 Fragments arranged by size: 3810 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5938 StyI C'CwwG_G Cuts at: 513 1065 2085 2597 3067 3377 513 Size: 552 1020 512 470 310 3074 Fragments arranged by size: 3074 1020 552 512 470 310 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2098 3053 3965 3982 4135 4320 4765 Size: 1255 518 955 912 17 153 185 445 Cuts at: 4765 325 Size: 1498 Fragments arranged by size: 1498 1255 955 912 518 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2982 4184 5896 Size: 274 80 33 51 1443 1005 1202 1712 Cuts at: 5896 96 Size: 138 Fragments arranged by size: 1712 1443 1202 1005 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2654 2686 5898 5930 2654 Size: 32 3212 32 2662 Fragments arranged by size: 3212 2662 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5938 XbaI T'CTAG_A Cuts at: 3274 3274 Size: 5938 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2088 2092 3065 3217 2088 Size: 4 973 152 4809 Fragments arranged by size: 4809 973 152 4 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 2802 3263 1870 Size: 932 461 4545 Fragments arranged by size: 4545 932 461 XmnI GAAnn'nnTTC Cuts at: 2696 4067 2696 Size: 1371 4567 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3115 3746 276 Size: 53 83 186 936 1581 631 2468 Fragments arranged by size: 2468 1581 936 631 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI