(Circular) (Six-base) MAPSORT of: pFN21AB7834.seq Check: 1483 from: 1 to: 6538 pFN21AB7834 6538 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3097 4348 278 Size: 53 83 186 936 1561 1251 2468 Fragments arranged by size: 2468 1561 1251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3881 1937 Size: 1944 4594 Acc65I G'GTAC_C Cuts at: 3859 3859 Size: 6538 AclI AA'CG_TT Cuts at: 1563 4665 5038 1563 Size: 3102 373 3063 Fragments arranged by size: 3102 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2501 2940 3093 3674 4601 5985 6253 1632 Size: 869 439 153 581 927 1384 268 1917 Fragments arranged by size: 1917 1384 927 869 581 439 268 153 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6341 AflIII A'CryG_T Cuts at: 1203 2472 5437 6414 1203 Size: 1269 2965 977 1327 Fragments arranged by size: 2965 1327 1269 977 AgeI A'CCGG_T Cuts at: 5369 5369 Size: 6538 AhdI GACnn_n'nnGTC Cuts at: 2241 5267 2241 Size: 3026 3512 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6538 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6538 AlwNI CAG_nnn'CTG Cuts at: 2315 3570 3742 4281 5853 2315 Size: 1255 172 539 1572 3000 Fragments arranged by size: 3000 1572 1255 539 172 ApaLI G'TGCA_C Cuts at: 4595 5751 4595 Size: 1156 5382 ApoI r'AATT_y Cuts at: 1493 2818 3110 3397 3847 4073 4225 6334 1493 Size: 1325 292 287 450 226 152 2109 1697 Fragments arranged by size: 2109 1697 1325 450 292 287 226 152 AseI AT'TA_AT Cuts at: 160 1032 5092 160 Size: 872 4060 1606 Fragments arranged by size: 4060 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 AvaI C'yCGr_G Cuts at: 1870 1943 2075 2124 2949 3863 1870 Size: 73 132 49 825 914 4545 Fragments arranged by size: 4545 914 825 132 73 49 BamHI G'GATC_C Cuts at: 3799 3868 4235 3799 Size: 69 367 6102 Fragments arranged by size: 6102 367 69 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2751 2758 3859 5314 618 Size: 325 217 627 964 7 1101 1455 1842 Fragments arranged by size: 1842 1455 1101 964 627 325 217 7 BanII G_rGCy'C Cuts at: 1391 3324 3857 1391 Size: 1933 533 4072 Fragments arranged by size: 4072 1933 533 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5567 BbvCI CC'TCA_GC Cuts at: 2140 2140 Size: 6538 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4729 4763 1903 Size: 34 50 34 2708 34 3678 Fragments arranged by size: 3678 2708 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3437 4430 5640 3437 Size: 993 1210 4335 Fragments arranged by size: 4335 1210 993 BclI T'GATC_A Cuts at: 1548 2362 1548 Size: 814 5724 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3767 5149 136 Size: 107 122 71 631 605 2095 1382 1525 Fragments arranged by size: 2095 1525 1382 631 605 122 107 71 BglII A'GATC_T Cuts at: 2071 3249 3541 6533 2071 Size: 1178 292 2992 2076 Fragments arranged by size: 2992 2076 1178 292 BlpI GC'TnA_GC Cuts at: 2150 3955 2150 Size: 1805 4733 Bme1580I G_kGCm'C Cuts at: 1790 3836 4599 5755 1790 Size: 2046 763 1156 2573 Fragments arranged by size: 2573 2046 1156 763 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6538 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2255 4269 5227 Size: 416 53 83 399 24 838 2014 958 Cuts at: 5227 442 Size: 1753 Fragments arranged by size: 2014 1753 958 838 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6538 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2416 5198 1239 Size: 1177 2782 2579 Fragments arranged by size: 2782 2579 1177 Bpu10I CC'TnA_GC Cuts at: 2140 2140 Size: 6538 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2148 4663 5528 5826 2148 Size: 2515 865 298 2860 Fragments arranged by size: 2860 2515 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5201 882 Size: 617 3702 2219 Fragments arranged by size: 3702 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4329 493 Size: 711 3125 2702 Fragments arranged by size: 3125 2702 711 BsaBI GATnn'nnATC Cuts at: 3623 6532 3623 Size: 2909 3629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3094 4345 4727 5362 Size: 53 83 186 936 1561 1251 382 635 Cuts at: 5362 275 Size: 1451 Fragments arranged by size: 1561 1451 1251 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4971 5369 5643 5790 6201 4971 Size: 398 274 147 411 5308 Fragments arranged by size: 5308 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3404 3434 1178 Size: 30 165 30 2001 30 4282 Fragments arranged by size: 4282 2001 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2128 2222 1186 Size: 942 94 5502 Fragments arranged by size: 5502 942 94 BseYI C'CCAG_C Cuts at: 3088 3803 5741 6237 3088 Size: 715 1938 496 3389 Fragments arranged by size: 3389 1938 715 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5965 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3988 4749 5777 1540 Size: 275 186 1987 761 1028 2301 Fragments arranged by size: 2301 1987 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2082 2677 2944 4599 4684 5755 2082 Size: 595 267 1655 85 1071 2865 Fragments arranged by size: 2865 1655 1071 595 267 85 BsmI GAATG_Cn' Cuts at: 2029 2666 4049 4142 2029 Size: 637 1383 93 4425 Fragments arranged by size: 4425 1383 637 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3101 3424 3638 3101 Size: 323 214 6001 Fragments arranged by size: 6001 323 214 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2677 2944 3324 3836 3857 4599 Size: 399 292 595 267 380 512 21 742 Cuts at: 4599 4684 5755 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 595 512 399 380 292 267 85 21 BspEI T'CCGG_A Cuts at: 6201 6201 Size: 6538 BspHI T'CATG_A Cuts at: 2365 4427 6157 2365 Size: 2062 1730 2746 Fragments arranged by size: 2746 2062 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3603 3893 844 Size: 2759 290 3489 Fragments arranged by size: 3489 2759 290 BsrBI CCG'CTC Cuts at: 1394 1823 2136 4425 1394 Size: 429 313 2289 3507 Fragments arranged by size: 3507 2289 429 313 BsrDI GCAATG_nn' Cuts at: 65 1248 2460 5033 5207 65 Size: 1183 1212 2573 174 1396 Fragments arranged by size: 2573 1396 1212 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3150 5182 5369 1700 Size: 110 1340 2032 187 2869 Fragments arranged by size: 2869 2032 1340 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6538 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6538 BssSI C'ACGA_G Cuts at: 4598 5610 4598 Size: 1012 5526 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6538 BstBI TT'CG_AA Cuts at: 2182 5342 2182 Size: 3160 3378 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6538 BstYI r'GATC_y Cuts at: 1972 2071 2926 3249 3541 3799 3868 4235 4620 Size: 99 855 323 292 258 69 367 385 Cuts at: 4620 4637 6089 6175 6187 6533 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 855 385 367 346 323 292 258 99 86 69 17 12 BtgI C'CryG_G Cuts at: 513 1065 3795 513 Size: 552 2730 3256 Fragments arranged by size: 3256 2730 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2492 487 Size: 683 56 1266 4533 Fragments arranged by size: 4533 1266 683 56 BtsI GCAGTG_nn' Cuts at: 2500 4054 4848 4868 2500 Size: 1554 794 20 4170 Fragments arranged by size: 4170 1554 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6538 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6503 DraI TTT'AAA Cuts at: 3120 3729 3842 3120 Size: 609 113 5816 Fragments arranged by size: 5816 609 113 DraIII CAC_nnn'GTG Cuts at: 3188 3188 Size: 6538 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5545 809 Size: 507 4229 1802 Fragments arranged by size: 4229 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3031 3159 3276 Size: 54 1425 50 275 18 1201 128 117 Cuts at: 3276 3985 4874 6503 8 Size: 709 889 1629 43 Fragments arranged by size: 1629 1425 1201 889 709 275 128 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3985 1537 Size: 275 2173 4090 Fragments arranged by size: 4090 2173 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2012 2224 2289 2932 3125 4468 1353 Size: 659 212 65 643 193 1343 3423 Fragments arranged by size: 3423 1343 659 643 212 193 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3137 3365 5125 5499 5645 387 Size: 1064 138 1548 228 1760 374 146 1280 Fragments arranged by size: 1760 1548 1280 1064 374 228 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2416 2501 2940 3093 3674 4601 5198 Size: 393 784 85 439 153 581 927 597 Cuts at: 5198 5985 6253 1239 Size: 787 268 1524 Fragments arranged by size: 1524 927 787 784 597 581 439 393 268 153 85 EcoNI CCTnn'n_nnAGG Cuts at: 2143 2565 2143 Size: 422 6116 EcoO109I rG'GnC_Cy Cuts at: 3739 3739 Size: 6538 EcoRI G'AATT_C Cuts at: 3110 3397 3847 4225 3110 Size: 287 450 378 5423 Fragments arranged by size: 5423 450 378 287 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3599 3599 Size: 6538 FspI TGC'GCA Cuts at: 5044 5044 Size: 6538 HaeII r_GCGC'y Cuts at: 1678 2521 5685 1678 Size: 843 3164 2531 Fragments arranged by size: 3164 2531 843 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2227 2259 2698 Size: 32 135 32 521 32 102 32 439 Cuts at: 2698 2730 5179 5211 5253 5285 1373 Size: 32 2449 32 42 32 2626 Fragments arranged by size: 2626 2449 521 439 135 102 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3882 4128 669 Size: 988 281 1944 246 3079 Fragments arranged by size: 3079 1944 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6538 HpaI GTT'AAC Cuts at: 4128 4128 Size: 6538 KpnI G_GTAC'C Cuts at: 3863 3863 Size: 6538 MfeI C'AATT_G Cuts at: 3021 4137 3021 Size: 1116 5422 MluI A'CGCG_T Cuts at: 6414 6414 Size: 6538 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2414 3804 5652 5836 1305 Size: 1109 1390 1848 184 2007 Fragments arranged by size: 2007 1848 1390 1109 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3161 3278 10 Size: 54 1425 343 1329 117 3270 Fragments arranged by size: 3270 1425 1329 343 117 54 MslI CAynn'nnrTG Cuts at: 518 1166 4496 4855 5014 518 Size: 648 3330 359 159 2042 Fragments arranged by size: 3330 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2360 2654 3807 3954 4631 5779 6024 1606 Size: 754 294 1153 147 677 1148 245 2120 Fragments arranged by size: 2120 1153 1148 754 677 294 245 147 NaeI GCC'GGC Cuts at: 3152 3152 Size: 6538 NcoI C'CATG_G Cuts at: 513 1065 3795 513 Size: 552 2730 3256 Fragments arranged by size: 3256 2730 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6538 NgoMIV G'CCGG_C Cuts at: 3150 3150 Size: 6538 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6538 NotI GC'GGCC_GC Cuts at: 3985 3985 Size: 6538 NspI r_CATG'y Cuts at: 1132 1229 2476 2744 3896 5441 1132 Size: 97 1247 268 1152 1545 2229 Fragments arranged by size: 2229 1545 1247 1152 268 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6538 PciI A'CATG_T Cuts at: 2472 5437 2472 Size: 2965 3573 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6538 PmeI GTTT'AAAC Cuts at: 3842 3842 Size: 6538 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3519 3551 4607 4639 6120 6152 3519 Size: 32 1056 32 1481 32 3905 Fragments arranged by size: 3905 1481 1056 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3739 3739 Size: 6538 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6538 PsiI TTA'TAA Cuts at: 3554 4108 3554 Size: 554 5984 PspXI vC'TCGA_Gb Cuts at: 2124 2124 Size: 6538 PstI C_TGCA'G Cuts at: 830 2193 3890 830 Size: 1363 1697 3478 Fragments arranged by size: 3478 1697 1363 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6538 PvuII CAG'CTG Cuts at: 2360 2654 3807 2360 Size: 294 1153 5091 Fragments arranged by size: 5091 1153 294 SalI G'TCGA_C Cuts at: 1936 3880 1936 Size: 1944 4594 SapI GCTCTTCn'nnn_ Cuts at: 2932 3125 2932 Size: 193 6345 SbfI CC_TGCA'GG Cuts at: 3890 3890 Size: 6538 ScaI AGT'ACT Cuts at: 3582 4786 6498 3582 Size: 1204 1712 3622 Fragments arranged by size: 3622 1712 1204 SexAI A'CCwGG_T Cuts at: 3050 3184 3050 Size: 134 6404 SfcI C'TryA_G Cuts at: 826 1045 2067 2189 3886 5021 5702 5893 826 Size: 219 1022 122 1697 1135 681 191 1471 Fragments arranged by size: 1697 1471 1135 1022 681 219 191 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6538 SmaI CCC'GGG Cuts at: 1872 2951 3865 1872 Size: 1079 914 4545 Fragments arranged by size: 4545 1079 914 SmlI C'TyrA_G Cuts at: 820 1017 1943 2075 2124 2163 4642 5543 5805 Size: 197 926 132 49 39 2479 901 262 Cuts at: 5805 820 Size: 1553 Fragments arranged by size: 2479 1553 926 901 262 197 132 49 39 SnaBI TAC'GTA Cuts at: 493 4329 493 Size: 3836 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6538 SphI G_CATG'C Cuts at: 1132 2744 3896 1132 Size: 1612 1152 3774 Fragments arranged by size: 3774 1612 1152 SrfI GCCC'GGGC Cuts at: 2951 2951 Size: 6538 SspI AAT'ATT Cuts at: 5 52 4462 5 Size: 47 4410 2081 Fragments arranged by size: 4410 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6538 StyI C'CwwG_G Cuts at: 513 1065 2466 3795 3828 3977 513 Size: 552 1401 1329 33 149 3074 Fragments arranged by size: 3074 1401 1329 552 149 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3195 4565 4582 4735 4920 5365 325 Size: 1255 1615 1370 17 153 185 445 1498 Fragments arranged by size: 1615 1498 1370 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3444 3580 4784 Size: 274 80 33 51 1443 1467 136 1204 Cuts at: 4784 6496 96 Size: 1712 138 Fragments arranged by size: 1712 1467 1443 1204 274 138 136 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6498 6530 6498 Size: 32 6506 Tth111I GACn'n_nGTC Cuts at: 1316 3668 1316 Size: 2352 4186 XbaI T'CTAG_A Cuts at: 3874 3874 Size: 6538 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3286 3793 3286 Size: 507 6031 XhoI C'TCGA_G Cuts at: 1943 2075 2124 1943 Size: 132 49 6357 Fragments arranged by size: 6357 132 49 XmaI C'CCGG_G Cuts at: 1870 2949 3863 1870 Size: 1079 914 4545 Fragments arranged by size: 4545 1079 914 XmnI GAAnn'nnTTC Cuts at: 3401 3582 4667 3401 Size: 181 1085 5272 Fragments arranged by size: 5272 1085 181 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3095 4346 276 Size: 53 83 186 936 1561 1251 2468 Fragments arranged by size: 2468 1561 1251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SanDI SfiI SfoI SwaI