(Circular) (Six-base) MAPSORT of: pFN21AB8882.seq Check: 626 from: 1 to: 5251 pFN21AB8882 5251 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3061 278 Size: 53 83 186 936 1525 2468 Fragments arranged by size: 2468 1525 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2385 2484 2594 1937 Size: 448 99 110 4594 Fragments arranged by size: 4594 448 110 99 Acc65I G'GTAC_C Cuts at: 2572 2572 Size: 5251 AclI AA'CG_TT Cuts at: 1563 3378 3751 1563 Size: 1815 373 3063 Fragments arranged by size: 3063 1815 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3314 4698 4966 1632 Size: 1682 1384 268 1917 Fragments arranged by size: 1917 1682 1384 268 AfeI AGC'GCT Cuts at: 2087 2087 Size: 5251 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5054 AflIII A'CryG_T Cuts at: 1203 4150 5127 1203 Size: 2947 977 1327 Fragments arranged by size: 2947 1327 977 AgeI A'CCGG_T Cuts at: 4082 4082 Size: 5251 AhdI GACnn_n'nnGTC Cuts at: 3980 3980 Size: 5251 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5251 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5251 AlwNI CAG_nnn'CTG Cuts at: 2323 2994 4566 2323 Size: 671 1572 3008 Fragments arranged by size: 3008 1572 671 ApaLI G'TGCA_C Cuts at: 3308 4464 3308 Size: 1156 4095 ApoI r'AATT_y Cuts at: 1493 2054 2560 2786 2938 5047 1493 Size: 561 506 226 152 2109 1697 Fragments arranged by size: 2109 1697 561 506 226 152 AseI AT'TA_AT Cuts at: 160 1032 3805 160 Size: 872 2773 1606 Fragments arranged by size: 2773 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5251 AvaI C'yCGr_G Cuts at: 1870 1943 2065 2157 2346 2445 2576 1870 Size: 73 122 92 189 99 131 4545 Fragments arranged by size: 4545 189 131 122 99 92 73 BamHI G'GATC_C Cuts at: 2581 2948 2581 Size: 367 4884 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2310 2572 4027 618 Size: 325 217 627 523 262 1455 1842 Fragments arranged by size: 1842 1455 627 523 325 262 217 BanII G_rGCy'C Cuts at: 1391 2550 2570 1391 Size: 1159 20 4072 Fragments arranged by size: 4072 1159 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4280 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2337 2371 3442 3476 1903 Size: 34 400 34 1071 34 3678 Fragments arranged by size: 3678 1071 400 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3143 4353 3143 Size: 1210 4041 BclI T'GATC_A Cuts at: 1548 1548 Size: 5251 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 3862 136 Size: 107 122 71 631 605 364 1826 1525 Fragments arranged by size: 1826 1525 631 605 364 122 107 71 BglII A'GATC_T Cuts at: 5246 5246 Size: 5251 BlpI GC'TnA_GC Cuts at: 2668 2668 Size: 5251 Bme1580I G_kGCm'C Cuts at: 1790 3312 4468 1790 Size: 1522 1156 2573 Fragments arranged by size: 2573 1522 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5251 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2982 3940 442 Size: 416 53 83 399 24 1565 958 1753 Fragments arranged by size: 1753 1565 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5251 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2117 2426 2117 Size: 309 4942 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 3911 1239 Size: 1076 1596 2579 Fragments arranged by size: 2579 1596 1076 Bpu10I CC'TnA_GC Cuts at: 2204 2204 Size: 5251 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3376 4241 4539 3376 Size: 865 298 4088 Fragments arranged by size: 4088 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3914 882 Size: 617 2415 2219 Fragments arranged by size: 2415 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3042 493 Size: 711 1838 2702 Fragments arranged by size: 2702 1838 711 BsaBI GATnn'nnATC Cuts at: 5245 5245 Size: 5251 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3058 3440 4075 275 Size: 53 83 186 936 1525 382 635 1451 Fragments arranged by size: 1525 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3684 4082 4356 4503 4914 3684 Size: 398 274 147 411 4021 Fragments arranged by size: 4021 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5026 Fragments arranged by size: 5026 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2540 2543 1186 Size: 1354 3 3894 Fragments arranged by size: 3894 1354 3 BseYI C'CCAG_C Cuts at: 2167 2171 2316 4454 4950 2167 Size: 4 145 2138 496 2468 Fragments arranged by size: 2468 2138 496 145 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4678 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2701 3462 4490 1540 Size: 275 186 700 761 1028 2301 Fragments arranged by size: 2301 1028 761 700 275 186 BsiHKAI G_wGCw'C Cuts at: 3312 3397 4468 3312 Size: 85 1071 4095 Fragments arranged by size: 4095 1071 85 BsmI GAATG_Cn' Cuts at: 2762 2855 2762 Size: 93 5158 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2550 2570 3312 3397 4468 1391 Size: 399 760 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 760 742 399 85 20 BspEI T'CCGG_A Cuts at: 4914 4914 Size: 5251 BspHI T'CATG_A Cuts at: 3140 4870 3140 Size: 1730 3521 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2298 2606 844 Size: 1454 308 3489 Fragments arranged by size: 3489 1454 308 BsrBI CCG'CTC Cuts at: 1394 1823 2016 3138 1394 Size: 429 193 1122 3507 Fragments arranged by size: 3507 1122 429 193 BsrDI GCAATG_nn' Cuts at: 65 1248 3746 3920 65 Size: 1183 2498 174 1396 Fragments arranged by size: 2498 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3895 4082 1700 Size: 110 2085 187 2869 Fragments arranged by size: 2869 2085 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5251 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5251 BssSI C'ACGA_G Cuts at: 3311 4323 3311 Size: 1012 4239 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5251 BstBI TT'CG_AA Cuts at: 4055 4055 Size: 5251 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5251 BstYI r'GATC_y Cuts at: 1972 2115 2581 2948 3333 3350 4802 4888 4900 Size: 143 466 367 385 17 1452 86 12 Cuts at: 4900 5246 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 466 385 367 346 143 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2137 2332 513 Size: 552 1072 195 3432 Fragments arranged by size: 3432 1072 552 195 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4512 Fragments arranged by size: 4512 683 56 BtsI GCAGTG_nn' Cuts at: 2767 3561 3581 2767 Size: 794 20 4437 Fragments arranged by size: 4437 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5251 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5216 DraI TTT'AAA Cuts at: 2555 2555 Size: 5251 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2471 4258 809 Size: 507 1155 1787 1802 Fragments arranged by size: 1802 1787 1155 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2452 2698 Size: 54 1425 50 275 18 207 415 246 Cuts at: 2698 3587 5216 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 415 275 246 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2698 1537 Size: 275 886 4090 Fragments arranged by size: 4090 886 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 2517 3181 1353 Size: 753 411 664 3423 Fragments arranged by size: 3423 753 664 411 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3838 4212 4358 387 Size: 1064 138 2249 374 146 1280 Fragments arranged by size: 2249 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 3314 3911 4698 4966 1239 Size: 393 683 999 597 787 268 1524 Fragments arranged by size: 1524 999 787 683 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2294 2294 Size: 5251 EcoO109I rG'GnC_Cy Cuts at: 2193 2193 Size: 5251 EcoRI G'AATT_C Cuts at: 2560 2938 2560 Size: 378 4873 FspI TGC'GCA Cuts at: 3757 3757 Size: 5251 HaeII r_GCGC'y Cuts at: 1678 2089 2323 4398 1678 Size: 411 234 2075 2531 Fragments arranged by size: 2531 2075 411 234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3892 3924 3966 3998 1373 Size: 32 135 32 2320 32 42 32 2626 Fragments arranged by size: 2626 2320 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2386 2595 2841 669 Size: 988 281 448 209 246 3079 Fragments arranged by size: 3079 988 448 281 246 209 HindIII A'AGCT_T Cuts at: 748 748 Size: 5251 HpaI GTT'AAC Cuts at: 2841 2841 Size: 5251 KpnI G_GTAC'C Cuts at: 2576 2576 Size: 5251 MfeI C'AATT_G Cuts at: 2850 2850 Size: 5251 MluI A'CGCG_T Cuts at: 5127 5127 Size: 5251 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4365 4549 1305 Size: 3060 184 2007 Fragments arranged by size: 3060 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3429 Fragments arranged by size: 3429 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3209 3568 3727 518 Size: 648 2043 359 159 2042 Fragments arranged by size: 2043 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2171 2667 3344 4492 4737 1606 Size: 565 496 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 565 496 245 NcoI C'CATG_G Cuts at: 513 1065 2137 513 Size: 552 1072 3627 Fragments arranged by size: 3627 1072 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5251 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5251 NotI GC'GGCC_GC Cuts at: 2698 2698 Size: 5251 NspI r_CATG'y Cuts at: 1132 1229 2030 2609 4154 1132 Size: 97 801 579 1545 2229 Fragments arranged by size: 2229 1545 801 579 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5251 PciI A'CATG_T Cuts at: 4150 4150 Size: 5251 PflMI CCAn_nnn'nTGG Cuts at: 1108 2323 1108 Size: 1215 4036 PmeI GTTT'AAAC Cuts at: 2555 2555 Size: 5251 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2207 2239 3320 3352 4833 4865 2207 Size: 32 1081 32 1481 32 2593 Fragments arranged by size: 2593 1481 1081 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5251 PsiI TTA'TAA Cuts at: 2821 2821 Size: 5251 PstI C_TGCA'G Cuts at: 830 2603 830 Size: 1773 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5251 PvuII CAG'CTG Cuts at: 2171 2171 Size: 5251 SalI G'TCGA_C Cuts at: 1936 2384 2593 1936 Size: 448 209 4594 Fragments arranged by size: 4594 448 209 SbfI CC_TGCA'GG Cuts at: 2603 2603 Size: 5251 ScaI AGT'ACT Cuts at: 3499 5211 3499 Size: 1712 3539 SfcI C'TryA_G Cuts at: 826 1045 2599 3734 4415 4606 826 Size: 219 1554 1135 681 191 1471 Fragments arranged by size: 1554 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5251 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5251 SmaI CCC'GGG Cuts at: 1872 2578 1872 Size: 706 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3355 4256 4518 820 Size: 197 926 1412 901 262 1553 Fragments arranged by size: 1553 1412 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3042 493 Size: 2549 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5251 SphI G_CATG'C Cuts at: 1132 2609 1132 Size: 1477 3774 SspI AAT'ATT Cuts at: 5 52 3175 5 Size: 47 3123 2081 Fragments arranged by size: 3123 2081 47 StuI AGG'CCT Cuts at: 1517 2100 1517 Size: 583 4668 StyI C'CwwG_G Cuts at: 513 1065 2137 2690 513 Size: 552 1072 553 3074 Fragments arranged by size: 3074 1072 553 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2066 3278 3295 3448 3633 4078 325 Size: 1255 486 1212 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1212 486 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3497 5209 96 Size: 274 80 33 51 1443 1520 1712 138 Fragments arranged by size: 1712 1520 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5211 5243 5211 Size: 32 5219 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5251 XbaI T'CTAG_A Cuts at: 2587 2587 Size: 5251 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5251 XmaI C'CCGG_G Cuts at: 1870 2576 1870 Size: 706 4545 XmnI GAAnn'nnTTC Cuts at: 3380 3380 Size: 5251 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3059 276 Size: 53 83 186 936 1525 2468 Fragments arranged by size: 2468 1525 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI