Restriction map for
pFN21AE0473
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaHI BsaI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeI MaeIII
MfeI MflI MluI MluNI MroI
MscI MseI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AfaI 241 GTAC GT^AC
AocI 91 CCTGAGG CC^T[ACGT]AGG
ApaI 248 GGGCCC GGGCC^C
AvaII 194 GGACC G^G[AT]CC
BanI 388 GGCACC G^G[CT][GA]CC
BanII 248 GGGCCC G[GA]GC[CT]^C
BlpI 457 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 457 GCTGAGC GC^T[ACGT]AGC
BsaBI 210 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 191 ACCGGA [AT]^CCGG[AT]
BsgI 68 CTGCAC GTGCAG(16/14)
BsmAI 340 GAGAC GTCTC(1/5)
BsmBI 340 GAGACG CGTCTC(1/5)
BspMI 108 ACCTGC ACCTGC(4/8)
BsrDI 437 GCAATG GCAATG(2/0)
Bsu36I 91 CCTGAGG CC^T[ACGT]AGG
CelII 457 GCTGAGC GC^T[ACGT]AGC
DpnII 216 GATC ^GATC
EaeI 225 CGGCCA [CT]^GGCC[GA]
Eco81I 91 CCTGAGG CC^T[ACGT]AGG
EcoT14I 101 CCAAGG C^C[AT][AT]GG
HapII 192 CCGG C^CGG
HpaII 192 CCGG C^CGG
HphI 218 TCACC GGTGA(8/7)
MamI 210 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 216 GATC ^GATC
MslI 444 CACGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 517 CAGCTG C[AC]G^C[GT]G
MspI 192 CCGG C^CGG
NdeII 216 GATC ^GATC
PstI 358 CTGCAG CTGCA^G
PvuII 517 CAGCTG CAG^CTG
RsaI 241 GTAC GT^AC
Sau3AI 216 GATC ^GATC
SfcI 358 CTGCAG C^T[GA][CT]AG
StuI 173 AGGCCT AGG^CCT
StyI 101 CCAAGG C^C[AT][AT]GG
Tsp509I 556 AATT ^AATT
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# TWO CUT ENZYMES ( 17 enzymes) #
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BmyI 248 GGGCCC G[AGT]GC[ACT]^C
387 GGGCAC
BsiYI 228 CCAGTGCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
372 CCGCAACAAGG
BslI 228 CCAGTGCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
372 CCGCAACAAGG
Bsp1286I 248 GGGCCC G[AGT]GC[ACT]^C
387 GGGCAC
BsrI 202 ACTGG ACTGG(1/-1)
228 CCAGT
BssKI 252 CCTGG ^CC[ACGT]GG
483 CCTGG
BstNI 252 CCTGG CC^[AT]GG
483 CCTGG
DraII 248 GGGCCCT [GA]G^G[ACGT]CC[CT]
410 AGGGCCT
EarI 160 GAAGAG CTCTTC(1/4)
310 GAAGAG
EcoO109I 248 GGGCCCT [GA]G^G[ACGT]CC[CT]
410 AGGGCCT
EcoRII 252 CCTGG ^CC[AT]GG
483 CCTGG
Ksp632I 160 GAAGAG CTCTTC(1/4)
310 GAAGAG
MaeII 343 ACGT A^CGT
470 ACGT
MvaI 252 CCTGG CC^[AT]GG
483 CCTGG
NlaIII 450 CATG CATG^
582 CATG
ScrFI 252 CCTGG CC^[ACGT]GG
483 CCTGG
TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^
229 CAGTG
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AluI AlwNI BbvI BpmI
BsaJI BstF5I Cac8I Cfr13I DdeI
FauI Fnu4HI FokI HaeIII ItaI
MboII MnlI MwoI NlaIV Sau96I
SfaNI TseI