Restriction map for
pFN21AE0473
(Linear) (Six-base) MAPSORT of: KIEE0473.seq Check: 6025 from: 1 to: 591
KIEE0473.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 527 591
Size: 329 198 64
Fragments arranged by size:
329 198 64
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 491 554 591
Size: 491 63 37
Fragments arranged by size:
491 63 37
AlwNI CAG_nnn'CTG
Cuts at: 0 67 79 91 591
Size: 67 12 12 500
Fragments arranged by size:
500 67 12 12
ApaI G_GGCC'C
Cuts at: 0 252 591
Size: 252 339
BanI G'GyrC_C
Cuts at: 0 388 591
Size: 388 203
BanII G_rGCy'C
Cuts at: 0 252 591
Size: 252 339
BlpI GC'TnA_GC
Cuts at: 0 458 591
Size: 458 133
Bme1580I G_kGCm'C
Cuts at: 0 252 391 591
Size: 252 139 200
Fragments arranged by size:
252 200 139
BmgBI CAC'GTC
Cuts at: 0 471 591
Size: 471 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 38 44 224 591
Size: 32 6 6 180 367
Fragments arranged by size:
367 180 32 6 6
Bpu10I CC'TnA_GC
Cuts at: 0 17 591
Size: 17 574
BsaBI GATnn'nnATC
Cuts at: 0 214 591
Size: 214 377
BsaWI w'CCGG_w
Cuts at: 0 191 591
Size: 191 400
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 505 535 591
Size: 505 30 56
Fragments arranged by size:
505 56 30
BseYI C'CCAG_C
Cuts at: 0 84 291 526 591
Size: 84 207 235 65
Fragments arranged by size:
235 207 84 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 591
Size: 53 538
BsmBI CGTCTCn'nnnn_
Cuts at: 0 334 591
Size: 334 257
Bsp1286I G_dGCh'C
Cuts at: 0 252 391 591
Size: 252 139 200
Fragments arranged by size:
252 200 139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 591
Size: 117 474
BsrDI GCAATG_nn'
Cuts at: 0 444 591
Size: 444 147
Bsu36I CC'TnA_GG
Cuts at: 0 92 591
Size: 92 499
BtsI GCAGTG_nn'
Cuts at: 0 143 591
Size: 143 448
EaeI y'GGCC_r
Cuts at: 0 225 591
Size: 225 366
EarI CTCTTCn'nnn_
Cuts at: 0 155 305 591
Size: 155 150 286
Fragments arranged by size:
286 155 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 38 44 224 329 527 591
Size: 32 6 6 180 105 198 64
Fragments arranged by size:
198 180 105 64 32 6 6
EcoO109I rG'GnC_Cy
Cuts at: 0 249 411 591
Size: 249 162 180
Fragments arranged by size:
249 180 162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 156 591
Size: 156 435
MslI CAynn'nnrTG
Cuts at: 0 448 591
Size: 448 143
MspA1I CmG'CkG
Cuts at: 0 519 591
Size: 519 72
PasI CC'CwG_GG
Cuts at: 0 252 483 591
Size: 252 231 108
Fragments arranged by size:
252 231 108
PspOMI G'GGCC_C
Cuts at: 0 248 591
Size: 248 343
PstI C_TGCA'G
Cuts at: 0 362 591
Size: 362 229
PvuII CAG'CTG
Cuts at: 0 519 591
Size: 519 72
SfcI C'TryA_G
Cuts at: 0 358 591
Size: 358 233
StuI AGG'CCT
Cuts at: 0 175 591
Size: 175 416
StyI C'CwwG_G
Cuts at: 0 101 591
Size: 101 490
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 442 591
Size: 442 149
TatI w'GTAC_w
Cuts at: 0 240 591
Size: 240 351
Enzymes that do cut:
AcuI AlfI AlwNI ApaI BanI BanII BlpI Bme1580I
BmgBI BpmI Bpu10I BsaBI BsaWI BsaXI BseYI BsgI
BsmBI Bsp1286I BspMI BsrDI Bsu36I BtsI EaeI EarI
Eco57MI EcoO109I FalI MslI MspA1I PasI PspOMI PstI
PvuII SfcI StuI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmrI BmtI
BplI BpuEI BsaI BsaAI BsaHI BseRI BsiEI BsiHKAI
BsiWI BsmI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI