(Circular) (Six-base) MAPSORT of: pFN21AB8877.seq Check: 2070 from: 1 to: 5209 pFN21AB8877 5209 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3019 278 Size: 53 83 186 936 1483 2468 Fragments arranged by size: 2468 1483 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2552 1937 Size: 615 4594 Acc65I G'GTAC_C Cuts at: 2530 2530 Size: 5209 AclI AA'CG_TT Cuts at: 1563 3336 3709 1563 Size: 1773 373 3063 Fragments arranged by size: 3063 1773 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3272 4656 4924 1632 Size: 1640 1384 268 1917 Fragments arranged by size: 1917 1640 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5012 AflIII A'CryG_T Cuts at: 1203 4108 5085 1203 Size: 2905 977 1327 Fragments arranged by size: 2905 1327 977 AgeI A'CCGG_T Cuts at: 4040 4040 Size: 5209 AhdI GACnn_n'nnGTC Cuts at: 3938 3938 Size: 5209 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5209 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2349 1184 Size: 1165 4044 AlwNI CAG_nnn'CTG Cuts at: 2952 4524 2952 Size: 1572 3637 ApaLI G'TGCA_C Cuts at: 3266 4422 3266 Size: 1156 4053 ApoI r'AATT_y Cuts at: 1493 2518 2744 2896 5005 1493 Size: 1025 226 152 2109 1697 Fragments arranged by size: 2109 1697 1025 226 152 AseI AT'TA_AT Cuts at: 160 1032 3763 160 Size: 872 2731 1606 Fragments arranged by size: 2731 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5209 AvaI C'yCGr_G Cuts at: 1870 1943 2153 2534 1870 Size: 73 210 381 4545 Fragments arranged by size: 4545 381 210 73 BamHI G'GATC_C Cuts at: 2539 2906 2539 Size: 367 4842 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2530 3985 618 Size: 325 217 627 743 1455 1842 Fragments arranged by size: 1842 1455 743 627 325 217 BanII G_rGCy'C Cuts at: 1391 2528 1391 Size: 1137 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3400 3434 1903 Size: 34 1463 34 3678 Fragments arranged by size: 3678 1463 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3101 4311 3101 Size: 1210 3999 BclI T'GATC_A Cuts at: 1548 1548 Size: 5209 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3820 136 Size: 107 122 71 631 605 2148 1525 Fragments arranged by size: 2148 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5204 5204 Size: 5209 BlpI GC'TnA_GC Cuts at: 2626 2626 Size: 5209 Bme1580I G_kGCm'C Cuts at: 1790 3270 4426 1790 Size: 1480 1156 2573 Fragments arranged by size: 2573 1480 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5209 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2940 3898 442 Size: 416 53 83 399 24 1523 958 1753 Fragments arranged by size: 1753 1523 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5209 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 3869 1239 Size: 1055 1575 2579 Fragments arranged by size: 2579 1575 1055 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3334 4199 4497 3334 Size: 865 298 4046 Fragments arranged by size: 4046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3872 882 Size: 617 2373 2219 Fragments arranged by size: 2373 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3000 493 Size: 711 1796 2702 Fragments arranged by size: 2702 1796 711 BsaBI GATnn'nnATC Cuts at: 5203 5203 Size: 5209 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2033 3016 3398 4033 Size: 53 83 186 936 500 983 382 635 Cuts at: 4033 275 Size: 1451 Fragments arranged by size: 1451 983 936 635 500 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3642 4040 4314 4461 4872 3642 Size: 398 274 147 411 3979 Fragments arranged by size: 3979 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4984 Fragments arranged by size: 4984 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5209 BseYI C'CCAG_C Cuts at: 2486 4412 4908 2486 Size: 1926 496 2787 Fragments arranged by size: 2787 1926 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4636 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2659 3420 4448 1540 Size: 275 186 658 761 1028 2301 Fragments arranged by size: 2301 1028 761 658 275 186 BsiHKAI G_wGCw'C Cuts at: 3270 3355 4426 3270 Size: 85 1071 4053 Fragments arranged by size: 4053 1071 85 BsmI GAATG_Cn' Cuts at: 2720 2813 2720 Size: 93 5116 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2528 3270 3355 4426 1391 Size: 399 738 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 738 399 85 BspEI T'CCGG_A Cuts at: 4872 4872 Size: 5209 BspHI T'CATG_A Cuts at: 2227 3098 4828 2227 Size: 871 1730 2608 Fragments arranged by size: 2608 1730 871 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2122 2234 2325 2564 844 Size: 1278 112 91 239 3489 Fragments arranged by size: 3489 1278 239 112 91 BsrBI CCG'CTC Cuts at: 1394 1823 3096 1394 Size: 429 1273 3507 Fragments arranged by size: 3507 1273 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2395 3704 3878 65 Size: 1183 1147 1309 174 1396 Fragments arranged by size: 1396 1309 1183 1147 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3853 4040 1700 Size: 110 2043 187 2869 Fragments arranged by size: 2869 2043 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5209 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5209 BssSI C'ACGA_G Cuts at: 3269 4281 3269 Size: 1012 4197 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5209 BstBI TT'CG_AA Cuts at: 4013 4013 Size: 5209 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5209 BstXI CCAn_nnnn'nTGG Cuts at: 2392 2392 Size: 5209 BstYI r'GATC_y Cuts at: 1972 2539 2906 3291 3308 4760 4846 4858 5204 Size: 567 367 385 17 1452 86 12 346 Cuts at: 5204 1972 Size: 1977 Fragments arranged by size: 1977 1452 567 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3718 Fragments arranged by size: 3718 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4470 Fragments arranged by size: 4470 683 56 BtsI GCAGTG_nn' Cuts at: 2725 3519 3539 2725 Size: 794 20 4395 Fragments arranged by size: 4395 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5209 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5174 DraI TTT'AAA Cuts at: 2513 2513 Size: 5209 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4216 809 Size: 507 2900 1802 Fragments arranged by size: 2900 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2235 2382 Size: 54 1425 50 275 18 177 228 147 Cuts at: 2382 2433 2656 3545 5174 8 Size: 51 223 889 1629 43 Fragments arranged by size: 1629 1425 889 275 228 223 177 147 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2656 1537 Size: 275 844 4090 Fragments arranged by size: 4090 844 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3139 1353 Size: 1786 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3796 4170 4316 387 Size: 1064 138 2207 374 146 1280 Fragments arranged by size: 2207 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 3272 3869 4656 4924 1239 Size: 393 662 978 597 787 268 1524 Fragments arranged by size: 1524 978 787 662 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2471 2471 Size: 5209 EcoO109I rG'GnC_Cy Cuts at: 2053 2053 Size: 5209 EcoRI G'AATT_C Cuts at: 2518 2896 2518 Size: 378 4831 FspI TGC'GCA Cuts at: 3715 3715 Size: 5209 HaeII r_GCGC'y Cuts at: 1678 4356 1678 Size: 2678 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 3850 3882 3924 Size: 32 135 32 446 32 1800 32 42 Cuts at: 3924 3956 1373 Size: 32 2626 Fragments arranged by size: 2626 1800 446 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2293 2553 2799 669 Size: 988 281 355 260 246 3079 Fragments arranged by size: 3079 988 355 281 260 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5209 HpaI GTT'AAC Cuts at: 2293 2799 2293 Size: 506 4703 KpnI G_GTAC'C Cuts at: 2534 2534 Size: 5209 MfeI C'AATT_G Cuts at: 2808 2808 Size: 5209 MluI A'CGCG_T Cuts at: 5085 5085 Size: 5209 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4323 4507 1305 Size: 3018 184 2007 Fragments arranged by size: 3018 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 10 Size: 54 1425 343 603 2784 Fragments arranged by size: 2784 1425 603 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3167 3526 3685 518 Size: 648 2001 359 159 2042 Fragments arranged by size: 2042 2001 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2042 2625 3302 4450 4695 1606 Size: 436 583 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 583 436 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3718 Fragments arranged by size: 3718 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5209 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5209 NotI GC'GGCC_GC Cuts at: 2656 2656 Size: 5209 NspI r_CATG'y Cuts at: 1132 1229 2567 4112 1132 Size: 97 1338 1545 2229 Fragments arranged by size: 2229 1545 1338 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5209 PciI A'CATG_T Cuts at: 4108 4108 Size: 5209 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5209 PfoI T'CCnGG_A Cuts at: 2250 2250 Size: 5209 PmeI GTTT'AAAC Cuts at: 2513 2513 Size: 5209 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3278 3310 4791 4823 3278 Size: 32 1481 32 3664 Fragments arranged by size: 3664 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2053 2053 Size: 5209 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5209 PsiI TTA'TAA Cuts at: 2779 2779 Size: 5209 PstI C_TGCA'G Cuts at: 830 2133 2561 830 Size: 1303 428 3478 Fragments arranged by size: 3478 1303 428 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5209 PvuII CAG'CTG Cuts at: 2042 2042 Size: 5209 SalI G'TCGA_C Cuts at: 1936 2551 1936 Size: 615 4594 SbfI CC_TGCA'GG Cuts at: 2561 2561 Size: 5209 ScaI AGT'ACT Cuts at: 3457 5169 3457 Size: 1712 3497 SfcI C'TryA_G Cuts at: 826 1045 2129 2557 3692 4373 4564 826 Size: 219 1084 428 1135 681 191 1471 Fragments arranged by size: 1471 1135 1084 681 428 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5209 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5209 SmaI CCC'GGG Cuts at: 1872 2536 1872 Size: 664 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3313 4214 4476 820 Size: 197 926 1370 901 262 1553 Fragments arranged by size: 1553 1370 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3000 493 Size: 2507 2702 SpeI A'CTAG_T Cuts at: 152 2069 152 Size: 1917 3292 SphI G_CATG'C Cuts at: 1132 2567 1132 Size: 1435 3774 SspI AAT'ATT Cuts at: 5 52 3133 5 Size: 47 3081 2081 Fragments arranged by size: 3081 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5209 StyI C'CwwG_G Cuts at: 513 1065 2004 2015 2648 513 Size: 552 939 11 633 3074 Fragments arranged by size: 3074 939 633 552 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2220 3236 3253 3406 3591 4036 325 Size: 1255 640 1016 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1016 640 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3455 5167 96 Size: 274 80 33 51 1443 1478 1712 138 Fragments arranged by size: 1712 1478 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5169 5201 5169 Size: 32 5177 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5209 XbaI T'CTAG_A Cuts at: 2545 2545 Size: 5209 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5209 XmaI C'CCGG_G Cuts at: 1870 2534 1870 Size: 664 4545 XmnI GAAnn'nnTTC Cuts at: 3338 3338 Size: 5209 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3017 276 Size: 53 83 186 936 1483 2468 Fragments arranged by size: 2468 1483 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI