(Circular) (Six-base) MAPSORT of: pFN21AB7880.seq Check: 4072 from: 1 to: 6625 pFN21AB7880 6625 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4435 278 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3968 1937 Size: 2031 4594 Acc65I G'GTAC_C Cuts at: 3946 3946 Size: 6625 AclI AA'CG_TT Cuts at: 1563 4752 5125 1563 Size: 3189 373 3063 Fragments arranged by size: 3189 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3539 3601 4688 6072 6340 1632 Size: 1907 62 1087 1384 268 1917 Fragments arranged by size: 1917 1907 1384 1087 268 62 AfeI AGC'GCT Cuts at: 2019 2019 Size: 6625 AflII C'TTAA_G Cuts at: 820 1017 3470 820 Size: 197 2453 3975 Fragments arranged by size: 3975 2453 197 AflIII A'CryG_T Cuts at: 1203 5524 6501 1203 Size: 4321 977 1327 Fragments arranged by size: 4321 1327 977 AgeI A'CCGG_T Cuts at: 5456 5456 Size: 6625 AhdI GACnn_n'nnGTC Cuts at: 5354 5354 Size: 6625 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6625 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2522 1184 Size: 1338 5287 AlwNI CAG_nnn'CTG Cuts at: 3435 4368 5940 3435 Size: 933 1572 4120 Fragments arranged by size: 4120 1572 933 ApaLI G'TGCA_C Cuts at: 4682 5838 4682 Size: 1156 5469 ApoI r'AATT_y Cuts at: 1493 2526 2892 3315 3399 3934 4160 4312 6421 Size: 1033 366 423 84 535 226 152 2109 Cuts at: 6421 1493 Size: 1697 Fragments arranged by size: 2109 1697 1033 535 423 366 226 152 84 AseI AT'TA_AT Cuts at: 160 1032 5179 160 Size: 872 4147 1606 Fragments arranged by size: 4147 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 AvaI C'yCGr_G Cuts at: 1870 1943 3950 1870 Size: 73 2007 4545 Fragments arranged by size: 4545 2007 73 BamHI G'GATC_C Cuts at: 3955 4322 3955 Size: 367 6258 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2151 2880 3894 3946 5401 Size: 325 217 627 364 729 1014 52 1455 Cuts at: 5401 618 Size: 1842 Fragments arranged by size: 1842 1455 1014 729 627 364 325 217 52 BanII G_rGCy'C Cuts at: 1391 2948 3924 3944 1391 Size: 1557 976 20 4072 Fragments arranged by size: 4072 1557 976 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3607 928 Size: 971 1708 3946 Fragments arranged by size: 3946 1708 971 BbvCI CC'TCA_GC Cuts at: 2226 2226 Size: 6625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4816 4850 1903 Size: 34 2879 34 3678 Fragments arranged by size: 3678 2879 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2342 4517 5727 2342 Size: 2175 1210 3240 Fragments arranged by size: 3240 2175 1210 BclI T'GATC_A Cuts at: 1548 3220 1548 Size: 1672 4953 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5236 136 Size: 107 122 71 631 605 3564 1525 Fragments arranged by size: 3564 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6620 6620 Size: 6625 BlpI GC'TnA_GC Cuts at: 4042 4042 Size: 6625 Bme1580I G_kGCm'C Cuts at: 1790 2883 4686 5842 1790 Size: 1093 1803 1156 2573 Fragments arranged by size: 2573 1803 1156 1093 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6625 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4356 5314 442 Size: 416 53 83 399 24 2939 958 1753 Fragments arranged by size: 2939 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6625 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 2751 3093 3177 3345 3429 5285 1239 Size: 956 556 342 84 168 84 1856 2579 Fragments arranged by size: 2579 1856 956 556 342 168 84 84 Bpu10I CC'TnA_GC Cuts at: 2226 2226 Size: 6625 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2962 3323 3560 3659 4750 5615 5913 2962 Size: 361 237 99 1091 865 298 3674 Fragments arranged by size: 3674 1091 865 361 298 237 99 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2642 5288 882 Size: 617 1143 2646 2219 Fragments arranged by size: 2646 2219 1143 617 BsaAI yAC'GTr Cuts at: 493 1204 4416 493 Size: 711 3212 2702 Fragments arranged by size: 3212 2702 711 BsaBI GATnn'nnATC Cuts at: 2330 6619 2330 Size: 4289 2336 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4432 4814 5449 275 Size: 53 83 186 936 2899 382 635 1451 Fragments arranged by size: 2899 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3827 5058 5456 5730 5877 6288 3827 Size: 1231 398 274 147 411 4164 Fragments arranged by size: 4164 1231 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6400 Fragments arranged by size: 6400 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2243 2507 1186 Size: 949 108 264 5304 Fragments arranged by size: 5304 949 264 108 BseYI C'CCAG_C Cuts at: 5828 6324 5828 Size: 496 6129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3196 3700 3748 1163 Size: 573 1460 504 48 4040 Fragments arranged by size: 4040 1460 573 504 48 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4075 4836 5864 1540 Size: 275 186 2074 761 1028 2301 Fragments arranged by size: 2301 2074 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2948 3309 3645 4686 4771 5842 2948 Size: 361 336 1041 85 1071 3731 Fragments arranged by size: 3731 1071 1041 361 336 85 BsmI GAATG_Cn' Cuts at: 3132 3431 3515 4136 4229 3132 Size: 299 84 621 93 5528 Fragments arranged by size: 5528 621 299 93 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2883 2948 3309 3645 3924 3944 4686 Size: 399 1093 65 361 336 279 20 742 Cuts at: 4686 4771 5842 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1093 1071 742 399 361 336 279 85 65 20 BspEI T'CCGG_A Cuts at: 6288 6288 Size: 6625 BspHI T'CATG_A Cuts at: 2161 2340 4514 6244 2161 Size: 179 2174 1730 2542 Fragments arranged by size: 2542 2174 1730 179 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2451 3980 844 Size: 1607 1529 3489 Fragments arranged by size: 3489 1607 1529 BsrBI CCG'CTC Cuts at: 1394 1823 3372 4512 1394 Size: 429 1549 1140 3507 Fragments arranged by size: 3507 1549 1140 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3013 3604 5120 5294 65 Size: 1183 1765 591 1516 174 1396 Fragments arranged by size: 1765 1516 1396 1183 591 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5269 5456 1700 Size: 110 3459 187 2869 Fragments arranged by size: 3459 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6625 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6625 BssSI C'ACGA_G Cuts at: 4685 5697 4685 Size: 1012 5613 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6625 BstBI TT'CG_AA Cuts at: 5429 5429 Size: 6625 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6625 BstXI CCAn_nnnn'nTGG Cuts at: 2468 2468 Size: 6625 BstYI r'GATC_y Cuts at: 1972 3955 4322 4707 4724 6176 6262 6274 6620 Size: 1983 367 385 17 1452 86 12 346 Cuts at: 6620 1972 Size: 1977 Fragments arranged by size: 1983 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 513 Size: 552 939 39 5095 Fragments arranged by size: 5095 939 552 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5886 Fragments arranged by size: 5886 683 56 BtsI GCAGTG_nn' Cuts at: 2506 3184 3436 3520 3688 3765 4141 4935 4955 Size: 678 252 84 168 77 376 794 20 Cuts at: 4955 2506 Size: 4176 Fragments arranged by size: 4176 794 678 376 252 168 84 77 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2824 2859 2995 3030 3250 3285 3586 Size: 35 2260 35 136 35 220 35 301 Cuts at: 3586 3621 529 Size: 35 3533 Fragments arranged by size: 3533 2260 301 220 136 35 35 35 35 35 DraI TTT'AAA Cuts at: 3929 3929 Size: 6625 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5632 809 Size: 507 4316 1802 Fragments arranged by size: 4316 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2409 3049 Size: 54 1425 50 275 18 177 402 640 Cuts at: 3049 4072 4961 6590 8 Size: 1023 889 1629 43 Fragments arranged by size: 1629 1425 1023 889 640 402 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4072 1537 Size: 275 2260 4090 Fragments arranged by size: 4090 2260 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 3912 4555 1353 Size: 696 1863 643 3423 Fragments arranged by size: 3423 1863 696 643 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3017 5212 5586 5732 387 Size: 1064 138 1428 2195 374 146 1280 Fragments arranged by size: 2195 1428 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2946 2946 Size: 6625 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2751 3093 3177 3345 3429 3539 Size: 393 563 556 342 84 168 84 110 Cuts at: 3539 3601 4688 5285 6072 6340 1239 Size: 62 1087 597 787 268 1524 Fragments arranged by size: 1524 1087 787 597 563 556 393 342 268 168 110 84 84 62 EcoO109I rG'GnC_Cy Cuts at: 2128 2233 2294 2128 Size: 105 61 6459 Fragments arranged by size: 6459 105 61 EcoRI G'AATT_C Cuts at: 2892 3315 3399 3934 4312 2892 Size: 423 84 535 378 5205 Fragments arranged by size: 5205 535 423 378 84 FspI TGC'GCA Cuts at: 2259 5131 2259 Size: 2872 3753 HaeII r_GCGC'y Cuts at: 1678 2021 5772 1678 Size: 343 3751 2531 Fragments arranged by size: 3751 2531 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5266 5298 5340 5372 1373 Size: 32 135 32 3694 32 42 32 2626 Fragments arranged by size: 3694 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3969 4215 669 Size: 988 281 2031 246 3079 Fragments arranged by size: 3079 2031 988 281 246 HindIII A'AGCT_T Cuts at: 748 2739 748 Size: 1991 4634 HpaI GTT'AAC Cuts at: 4215 4215 Size: 6625 KpnI G_GTAC'C Cuts at: 3950 3950 Size: 6625 MfeI C'AATT_G Cuts at: 4224 4224 Size: 6625 MluI A'CGCG_T Cuts at: 6501 6501 Size: 6625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2603 2623 5739 5923 1305 Size: 1298 20 3116 184 2007 Fragments arranged by size: 3116 2007 1298 184 20 MscI TGG'CCA Cuts at: 10 64 1489 1832 2411 3051 10 Size: 54 1425 343 579 640 3584 Fragments arranged by size: 3584 1425 640 579 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2319 3123 4583 4942 5101 518 Size: 648 1153 804 1460 359 159 2042 Fragments arranged by size: 2042 1460 1153 804 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4041 4718 5866 6111 1606 Size: 2435 677 1148 245 2120 Fragments arranged by size: 2435 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2043 513 Size: 552 939 39 5095 Fragments arranged by size: 5095 939 552 39 NdeI CA'TA_TG Cuts at: 387 3350 387 Size: 2963 3662 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6625 NotI GC'GGCC_GC Cuts at: 4072 4072 Size: 6625 NspI r_CATG'y Cuts at: 1132 1229 3388 3774 3983 5528 1132 Size: 97 2159 386 209 1545 2229 Fragments arranged by size: 2229 2159 1545 386 209 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6625 PciI A'CATG_T Cuts at: 5524 5524 Size: 6625 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6625 PmeI GTTT'AAAC Cuts at: 3929 3929 Size: 6625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4694 4726 6207 6239 4694 Size: 32 1481 32 5080 Fragments arranged by size: 5080 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2294 2294 Size: 6625 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6625 PsiI TTA'TAA Cuts at: 2745 3759 4195 2745 Size: 1014 436 5175 Fragments arranged by size: 5175 1014 436 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2765 2797 2765 Size: 32 6593 PstI C_TGCA'G Cuts at: 830 3977 830 Size: 3147 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6625 SacI G_AGCT'C Cuts at: 2948 2948 Size: 6625 SalI G'TCGA_C Cuts at: 1936 3967 1936 Size: 2031 4594 SapI GCTCTTCn'nnn_ Cuts at: 3912 3912 Size: 6625 SbfI CC_TGCA'GG Cuts at: 3977 3977 Size: 6625 ScaI AGT'ACT Cuts at: 4873 6585 4873 Size: 1712 4913 SfcI C'TryA_G Cuts at: 826 1045 3973 5108 5789 5980 826 Size: 219 2928 1135 681 191 1471 Fragments arranged by size: 2928 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6625 SmaI CCC'GGG Cuts at: 1872 3952 1872 Size: 2080 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2941 3302 3470 3539 3638 4729 Size: 197 926 998 361 168 69 99 1091 Cuts at: 4729 5630 5892 820 Size: 901 262 1553 Fragments arranged by size: 1553 1091 998 926 901 361 262 197 168 99 69 SnaBI TAC'GTA Cuts at: 493 4416 493 Size: 3923 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6625 SphI G_CATG'C Cuts at: 1132 3983 1132 Size: 2851 3774 SspI AAT'ATT Cuts at: 5 52 4549 5 Size: 47 4497 2081 Fragments arranged by size: 4497 2081 47 StuI AGG'CCT Cuts at: 1517 3082 3250 3278 3502 3754 3782 1517 Size: 1565 168 28 224 252 28 4360 Fragments arranged by size: 4360 1565 252 224 168 28 28 StyI C'CwwG_G Cuts at: 513 1065 2004 2043 4064 513 Size: 552 939 39 2021 3074 Fragments arranged by size: 3074 2021 939 552 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2627 4652 4669 4822 5007 5452 325 Size: 1255 1047 2025 17 153 185 445 1498 Fragments arranged by size: 2025 1498 1255 1047 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 4871 6583 Size: 274 80 33 51 1443 129 2765 1712 Cuts at: 6583 96 Size: 138 Fragments arranged by size: 2765 1712 1443 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6585 6617 6585 Size: 32 6593 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6625 XbaI T'CTAG_A Cuts at: 3961 3961 Size: 6625 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6625 XmaI C'CCGG_G Cuts at: 1870 3950 1870 Size: 2080 4545 XmnI GAAnn'nnTTC Cuts at: 2852 3023 3194 3278 3446 3866 4754 2852 Size: 171 171 84 168 420 888 4723 Fragments arranged by size: 4723 888 420 171 171 168 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4433 276 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI