(Circular) (Six-base) MAPSORT of: pFN21AB7766.seq Check: 9805 from: 1 to: 6214 pFN21AB7766 6214 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4024 278 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2323 3557 1937 Size: 386 1234 4594 Fragments arranged by size: 4594 1234 386 Acc65I G'GTAC_C Cuts at: 3535 3535 Size: 6214 AclI AA'CG_TT Cuts at: 1563 4341 4714 1563 Size: 2778 373 3063 Fragments arranged by size: 3063 2778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4277 5661 5929 1632 Size: 2645 1384 268 1917 Fragments arranged by size: 2645 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 5113 6090 1203 Size: 3910 977 1327 Fragments arranged by size: 3910 1327 977 AgeI A'CCGG_T Cuts at: 3390 5045 3390 Size: 1655 4559 AhdI GACnn_n'nnGTC Cuts at: 4943 4943 Size: 6214 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6214 AlwNI CAG_nnn'CTG Cuts at: 3957 5529 3957 Size: 1572 4642 ApaLI G'TGCA_C Cuts at: 4271 5427 4271 Size: 1156 5058 ApoI r'AATT_y Cuts at: 1493 2039 2083 2194 2332 2471 2479 2583 2748 Size: 546 44 111 138 139 8 104 165 Cuts at: 2748 2792 2832 2916 3000 3185 3252 3336 3523 Size: 44 40 84 84 185 67 84 187 Cuts at: 3523 3749 3901 6010 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 546 226 187 185 165 152 139 138 111 104 84 84 84 67 44 44 40 8 AseI AT'TA_AT Cuts at: 160 1032 4768 160 Size: 872 3736 1606 Fragments arranged by size: 3736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 3539 1870 Size: 73 1596 4545 Fragments arranged by size: 4545 1596 73 BamHI G'GATC_C Cuts at: 3544 3911 3544 Size: 367 5847 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3535 4990 618 Size: 325 217 627 1748 1455 1842 Fragments arranged by size: 1842 1748 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3533 1391 Size: 2142 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2119 2207 928 Size: 971 220 88 4935 Fragments arranged by size: 4935 971 220 88 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4405 4439 1903 Size: 34 2468 34 3678 Fragments arranged by size: 3678 2468 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4106 5316 4106 Size: 1210 5004 BclI T'GATC_A Cuts at: 1548 2160 1548 Size: 612 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4825 136 Size: 107 122 71 631 605 3153 1525 Fragments arranged by size: 3153 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6209 6209 Size: 6214 BlpI GC'TnA_GC Cuts at: 3631 3631 Size: 6214 Bme1580I G_kGCm'C Cuts at: 1790 4275 5431 1790 Size: 2485 1156 2573 Fragments arranged by size: 2573 2485 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6214 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2222 3945 4903 Size: 416 53 83 399 24 805 1723 958 Cuts at: 4903 442 Size: 1753 Fragments arranged by size: 1753 1723 958 805 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2192 2613 2778 2862 3114 3282 3366 Size: 830 123 421 165 84 252 168 84 Cuts at: 3366 4874 1239 Size: 1508 2579 Fragments arranged by size: 2579 1508 830 421 252 168 165 123 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4339 5204 5502 4339 Size: 865 298 5051 Fragments arranged by size: 5051 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4877 882 Size: 617 3378 2219 Fragments arranged by size: 3378 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4005 493 Size: 711 2801 2702 Fragments arranged by size: 2801 2702 711 BsaBI GATnn'nnATC Cuts at: 6208 6208 Size: 6214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4021 4403 5038 275 Size: 53 83 186 936 2488 382 635 1451 Fragments arranged by size: 2488 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3390 4647 5045 5319 5466 5877 3390 Size: 1257 398 274 147 411 3727 Fragments arranged by size: 3727 1257 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5989 Fragments arranged by size: 5989 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 5334 BseYI C'CCAG_C Cuts at: 5417 5913 5417 Size: 496 5718 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5641 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3664 4425 5453 1540 Size: 275 186 1663 761 1028 2301 Fragments arranged by size: 2301 1663 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4275 4360 5431 4275 Size: 85 1071 5058 Fragments arranged by size: 5058 1071 85 BsmI GAATG_Cn' Cuts at: 2553 3725 3818 2553 Size: 1172 93 4949 Fragments arranged by size: 4949 1172 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3533 4275 4360 5431 1391 Size: 399 1743 742 85 1071 2174 Fragments arranged by size: 2174 1743 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5877 5877 Size: 6214 BspHI T'CATG_A Cuts at: 4103 5833 4103 Size: 1730 4484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3569 844 Size: 2725 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4101 1394 Size: 429 2278 3507 Fragments arranged by size: 3507 2278 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2066 4709 4883 65 Size: 1183 818 2643 174 1396 Fragments arranged by size: 2643 1396 1183 818 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3390 4858 5045 1700 Size: 110 1580 1468 187 2869 Fragments arranged by size: 2869 1580 1468 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6214 BssSI C'ACGA_G Cuts at: 4274 5286 4274 Size: 1012 5202 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6214 BstBI TT'CG_AA Cuts at: 2510 5018 2510 Size: 2508 3706 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6214 BstYI r'GATC_y Cuts at: 1972 3544 3911 4296 4313 5765 5851 5863 6209 Size: 1572 367 385 17 1452 86 12 346 Cuts at: 6209 1972 Size: 1977 Fragments arranged by size: 1977 1572 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4723 Fragments arranged by size: 4723 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5475 Fragments arranged by size: 5475 683 56 BtsI GCAGTG_nn' Cuts at: 3730 4524 4544 3730 Size: 794 20 5400 Fragments arranged by size: 5400 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6179 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5221 809 Size: 507 3905 1802 Fragments arranged by size: 3905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2261 3661 Size: 54 1425 50 275 18 201 230 1400 Cuts at: 3661 4550 6179 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1400 889 275 230 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3661 1537 Size: 275 1849 4090 Fragments arranged by size: 4090 1849 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4144 1353 Size: 2791 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4801 5175 5321 387 Size: 1064 138 3212 374 146 1280 Fragments arranged by size: 3212 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2192 2613 2778 2862 3114 3282 Size: 393 437 123 421 165 84 252 168 Cuts at: 3282 3366 4277 4874 5661 5929 1239 Size: 84 911 597 787 268 1524 Fragments arranged by size: 1524 911 787 597 437 421 393 268 252 168 165 123 84 84 EcoRI G'AATT_C Cuts at: 2039 2748 2832 3000 3252 3336 3523 3901 2039 Size: 709 84 168 252 84 187 378 4352 Fragments arranged by size: 4352 709 378 252 187 168 84 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2523 2523 Size: 6214 FspI TGC'GCA Cuts at: 4720 4720 Size: 6214 HaeII r_GCGC'y Cuts at: 1678 5361 1678 Size: 3683 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2152 2184 4855 4887 4929 Size: 32 135 32 580 32 2671 32 42 Cuts at: 4929 4961 1373 Size: 32 2626 Fragments arranged by size: 2671 2626 580 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2016 3558 3804 669 Size: 988 281 78 1542 246 3079 Fragments arranged by size: 3079 1542 988 281 246 78 HindIII A'AGCT_T Cuts at: 748 2961 3045 3129 3381 748 Size: 2213 84 84 252 3581 Fragments arranged by size: 3581 2213 252 84 84 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KpnI G_GTAC'C Cuts at: 3539 3539 Size: 6214 MfeI C'AATT_G Cuts at: 3813 3813 Size: 6214 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5328 5512 1305 Size: 4023 184 2007 Fragments arranged by size: 4023 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2033 2263 10 Size: 54 1425 343 201 230 3961 Fragments arranged by size: 3961 1425 343 230 201 54 MslI CAynn'nnrTG Cuts at: 518 1166 2941 4172 4531 4690 518 Size: 648 1775 1231 359 159 2042 Fragments arranged by size: 2042 1775 1231 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3630 4307 5455 5700 1606 Size: 2024 677 1148 245 2120 Fragments arranged by size: 2120 2024 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4723 Fragments arranged by size: 4723 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 3572 5117 1132 Size: 97 2343 1545 2229 Fragments arranged by size: 2343 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6214 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6214 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4283 4315 5796 5828 4283 Size: 32 1481 32 4669 Fragments arranged by size: 4669 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6214 PsiI TTA'TAA Cuts at: 2394 3784 2394 Size: 1390 4824 PstI C_TGCA'G Cuts at: 830 3566 830 Size: 2736 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SbfI CC_TGCA'GG Cuts at: 3566 3566 Size: 6214 ScaI AGT'ACT Cuts at: 4462 6174 4462 Size: 1712 4502 SexAI A'CCwGG_T Cuts at: 2121 2121 Size: 6214 SfcI C'TryA_G Cuts at: 826 1045 2113 3177 3562 4697 5378 5569 826 Size: 219 1068 1064 385 1135 681 191 1471 Fragments arranged by size: 1471 1135 1068 1064 681 385 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 3541 1872 Size: 1669 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4318 5219 5481 820 Size: 197 926 2375 901 262 1553 Fragments arranged by size: 2375 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3572 1132 Size: 2440 3774 SspI AAT'ATT Cuts at: 5 52 2436 4138 5 Size: 47 2384 1702 2081 Fragments arranged by size: 2384 2081 1702 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6214 StyI C'CwwG_G Cuts at: 513 1065 2004 2189 3653 513 Size: 552 939 185 1464 3074 Fragments arranged by size: 3074 1464 939 552 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4241 4258 4411 4596 5041 325 Size: 1255 2661 17 153 185 445 1498 Fragments arranged by size: 2661 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2651 4460 6172 Size: 274 80 33 51 1443 674 1809 1712 Cuts at: 6172 96 Size: 138 Fragments arranged by size: 1809 1712 1443 674 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6174 6206 6174 Size: 32 6182 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6214 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6214 XmaI C'CCGG_G Cuts at: 1870 3539 1870 Size: 1669 4545 XmnI GAAnn'nnTTC Cuts at: 2519 4343 2519 Size: 1824 4390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4022 276 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI