(Circular) (Six-base) MAPSORT of: pFN21AE0027.seq Check: 3363 from: 1 to: 6415 pFN21AE0027 6415 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2151 4225 278 Size: 53 83 186 936 615 2074 2468 Fragments arranged by size: 2468 2074 936 615 186 83 53 AccI GT'mk_AC Cuts at: 1937 3758 1937 Size: 1821 4594 Acc65I G'GTAC_C Cuts at: 3736 3736 Size: 6415 AclI AA'CG_TT Cuts at: 1563 4542 4915 1563 Size: 2979 373 3063 Fragments arranged by size: 3063 2979 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 4478 5862 6130 1632 Size: 608 2238 1384 268 1917 Fragments arranged by size: 2238 1917 1384 608 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6218 AflIII A'CryG_T Cuts at: 1203 3230 5314 6291 1203 Size: 2027 2084 977 1327 Fragments arranged by size: 2084 2027 1327 977 AgeI A'CCGG_T Cuts at: 5246 5246 Size: 6415 AhdI GACnn_n'nnGTC Cuts at: 5144 5144 Size: 6415 AleI CACnn'nnGTG Cuts at: 1166 2991 1166 Size: 1825 4590 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2109 2892 2985 1184 Size: 925 783 93 4614 Fragments arranged by size: 4614 925 783 93 AlwNI CAG_nnn'CTG Cuts at: 2493 2695 3026 3562 4158 5730 2493 Size: 202 331 536 596 1572 3178 Fragments arranged by size: 3178 1572 596 536 331 202 ApaI G_GGCC'C Cuts at: 2020 2020 Size: 6415 ApaLI G'TGCA_C Cuts at: 2867 4472 5628 2867 Size: 1605 1156 3654 Fragments arranged by size: 3654 1605 1156 ApoI r'AATT_y Cuts at: 1493 3724 3950 4102 6211 1493 Size: 2231 226 152 2109 1697 Fragments arranged by size: 2231 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4969 160 Size: 872 3937 1606 Fragments arranged by size: 3937 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 AvaI C'yCGr_G Cuts at: 1870 1943 2019 2128 2933 3740 1870 Size: 73 76 109 805 807 4545 Fragments arranged by size: 4545 807 805 109 76 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2606 2639 2606 Size: 33 6382 BamHI G'GATC_C Cuts at: 3745 4112 3745 Size: 367 6048 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3736 5191 618 Size: 325 217 627 1949 1455 1842 Fragments arranged by size: 1949 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2020 2127 2237 2259 3734 1391 Size: 629 107 110 22 1475 4072 Fragments arranged by size: 4072 1475 629 110 107 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5444 BbvCI CC'TCA_GC Cuts at: 3257 3257 Size: 6415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4606 4640 1903 Size: 34 2669 34 3678 Fragments arranged by size: 3678 2669 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4307 5517 4307 Size: 1210 5205 BclI T'GATC_A Cuts at: 1548 1548 Size: 6415 BfrBI ATG'CAT Cuts at: 3329 3329 Size: 6415 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3002 5026 136 Size: 107 122 71 631 605 1330 2024 1525 Fragments arranged by size: 2024 1525 1330 631 605 122 107 71 BglII A'GATC_T Cuts at: 6410 6410 Size: 6415 BlpI GC'TnA_GC Cuts at: 3083 3832 3083 Size: 749 5666 Bme1580I G_kGCm'C Cuts at: 1790 2020 2144 2871 3532 4476 5632 1790 Size: 230 124 727 661 944 1156 2573 Fragments arranged by size: 2573 1156 944 727 661 230 124 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6415 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3238 3348 3512 Size: 416 53 83 399 24 1821 110 164 Cuts at: 3512 3676 4146 5104 442 Size: 164 470 958 1753 Fragments arranged by size: 1821 1753 958 470 416 399 164 164 110 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6415 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2534 2534 Size: 6415 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2416 2600 2657 3036 3126 3402 3648 5075 Size: 1177 184 57 379 90 276 246 1427 Cuts at: 5075 1239 Size: 2579 Fragments arranged by size: 2579 1427 1177 379 276 246 184 90 57 Bpu10I CC'TnA_GC Cuts at: 3257 3257 Size: 6415 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3436 4540 5405 5703 3436 Size: 1104 865 298 4148 Fragments arranged by size: 4148 1104 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2180 2867 3573 5078 882 Size: 617 681 687 706 1505 2219 Fragments arranged by size: 2219 1505 706 687 681 617 BsaAI yAC'GTr Cuts at: 493 1204 2659 4206 493 Size: 711 1455 1547 2702 Fragments arranged by size: 2702 1547 1455 711 BsaBI GATnn'nnATC Cuts at: 6409 6409 Size: 6415 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 4222 4604 5239 Size: 53 83 186 936 615 2074 382 635 Cuts at: 5239 275 Size: 1451 Fragments arranged by size: 2074 1451 936 635 615 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4848 5246 5520 5667 6078 4848 Size: 398 274 147 411 5185 Fragments arranged by size: 5185 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2521 2551 3489 3519 1178 Size: 30 165 30 1118 30 938 30 4074 Fragments arranged by size: 4074 1118 938 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3195 3420 1186 Size: 2009 225 4181 Fragments arranged by size: 4181 2009 225 BseYI C'CCAG_C Cuts at: 2098 3606 5618 6114 2098 Size: 1508 2012 496 2399 Fragments arranged by size: 2399 2012 1508 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2159 2385 2690 3536 1163 Size: 573 423 226 305 846 4042 Fragments arranged by size: 4042 846 573 423 305 226 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2112 3865 4626 5654 1540 Size: 275 186 111 1753 761 1028 2301 Fragments arranged by size: 2301 1753 1028 761 275 186 111 BsiHKAI G_wGCw'C Cuts at: 2031 2237 2871 3639 4476 4561 5632 2031 Size: 206 634 768 837 85 1071 2814 Fragments arranged by size: 2814 1071 837 768 634 206 85 BsiWI C'GTAC_G Cuts at: 2159 2159 Size: 6415 BsmI GAATG_Cn' Cuts at: 3926 4019 3926 Size: 93 6322 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2031 2127 2144 2237 2259 2871 Size: 399 230 11 96 17 93 22 612 Cuts at: 2871 3532 3639 3734 4476 4561 5632 1391 Size: 661 107 95 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 661 612 399 230 107 96 95 93 85 22 17 11 BspEI T'CCGG_A Cuts at: 6078 6078 Size: 6415 BspHI T'CATG_A Cuts at: 3387 4304 6034 3387 Size: 917 1730 3768 Fragments arranged by size: 3768 1730 917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2561 2712 2967 3770 844 Size: 1717 151 255 803 3489 Fragments arranged by size: 3489 1717 803 255 151 BsrBI CCG'CTC Cuts at: 1394 1823 4302 1394 Size: 429 2479 3507 Fragments arranged by size: 3507 2479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4910 5084 65 Size: 1183 3662 174 1396 Fragments arranged by size: 3662 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2054 5059 5246 1700 Size: 110 244 3005 187 2869 Fragments arranged by size: 3005 2869 244 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6415 BssHII G'CGCG_C Cuts at: 1925 2067 1925 Size: 142 6273 BssSI C'ACGA_G Cuts at: 2864 3707 4475 5487 2864 Size: 843 768 1012 3792 Fragments arranged by size: 3792 1012 843 768 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6415 BstBI TT'CG_AA Cuts at: 5219 5219 Size: 6415 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6415 BstXI CCAn_nnnn'nTGG Cuts at: 3619 3619 Size: 6415 BstYI r'GATC_y Cuts at: 1972 2215 2691 3745 4112 4497 4514 5966 6052 Size: 243 476 1054 367 385 17 1452 86 Cuts at: 6052 6064 6410 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1054 476 385 367 346 243 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3060 3191 513 Size: 552 939 1056 131 3737 Fragments arranged by size: 3737 1056 939 552 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5676 Fragments arranged by size: 5676 683 56 BtsI GCAGTG_nn' Cuts at: 2033 2986 2998 3510 3931 4725 4745 2033 Size: 953 12 512 421 794 20 3703 Fragments arranged by size: 3703 953 794 512 421 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6415 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6380 DraI TTT'AAA Cuts at: 3719 3719 Size: 6415 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3202 5422 809 Size: 507 1886 2220 1802 Fragments arranged by size: 2220 1886 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2179 2590 3573 Size: 54 1425 50 275 18 349 411 983 Cuts at: 3573 3862 4751 6380 8 Size: 289 889 1629 43 Fragments arranged by size: 1629 1425 983 889 411 349 289 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3862 1537 Size: 275 2050 4090 Fragments arranged by size: 4090 2050 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2625 3452 3547 4345 1353 Size: 1272 827 95 798 3423 Fragments arranged by size: 3423 1272 827 798 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5002 5376 5522 387 Size: 1064 138 3413 374 146 1280 Fragments arranged by size: 3413 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2235 2235 Size: 6415 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2416 2600 2657 3036 3126 3402 Size: 393 608 176 184 57 379 90 276 Cuts at: 3402 3648 4478 5075 5862 6130 1239 Size: 246 830 597 787 268 1524 Fragments arranged by size: 1524 830 787 608 597 393 379 276 268 246 184 176 90 57 EcoNI CCTnn'n_nnAGG Cuts at: 2243 2243 Size: 6415 EcoO109I rG'GnC_Cy Cuts at: 2016 2788 2016 Size: 772 5643 EcoRI G'AATT_C Cuts at: 3724 4102 3724 Size: 378 6037 FspI TGC'GCA Cuts at: 4921 4921 Size: 6415 HaeII r_GCGC'y Cuts at: 1678 3607 5562 1678 Size: 1929 1955 2531 Fragments arranged by size: 2531 1955 1929 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2193 2225 2702 2734 3108 Size: 32 135 32 621 32 477 32 374 Cuts at: 3108 3140 5056 5088 5130 5162 1373 Size: 32 1916 32 42 32 2626 Fragments arranged by size: 2626 1916 621 477 374 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3759 4005 669 Size: 988 281 1821 246 3079 Fragments arranged by size: 3079 1821 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6415 HpaI GTT'AAC Cuts at: 4005 4005 Size: 6415 KpnI G_GTAC'C Cuts at: 3740 3740 Size: 6415 MfeI C'AATT_G Cuts at: 4014 4014 Size: 6415 MluI A'CGCG_T Cuts at: 6291 6291 Size: 6415 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2256 5529 5713 1305 Size: 951 3273 184 2007 Fragments arranged by size: 3273 2007 951 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2592 3575 10 Size: 54 1425 343 760 983 2850 Fragments arranged by size: 2850 1425 983 760 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2537 2967 2991 3518 4373 4732 Size: 648 1044 327 430 24 527 855 359 Cuts at: 4732 4891 518 Size: 159 2042 Fragments arranged by size: 2042 1044 855 648 527 430 359 327 159 24 MspA1I CmG'CkG Cuts at: 1606 3831 4508 5656 5901 1606 Size: 2225 677 1148 245 2120 Fragments arranged by size: 2225 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3060 3191 513 Size: 552 939 1056 131 3737 Fragments arranged by size: 3737 1056 939 552 131 NdeI CA'TA_TG Cuts at: 387 387 Size: 6415 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6415 NotI GC'GGCC_GC Cuts at: 3862 3862 Size: 6415 NsiI A_TGCA'T Cuts at: 3331 3331 Size: 6415 NspI r_CATG'y Cuts at: 1132 1229 3082 3213 3279 3333 3773 5318 1132 Size: 97 1853 131 66 54 440 1545 2229 Fragments arranged by size: 2229 1853 1545 440 131 97 66 54 PasI CC'CwG_GG Cuts at: 1348 3299 1348 Size: 1951 4464 PciI A'CATG_T Cuts at: 5314 5314 Size: 6415 PflMI CCAn_nnn'nTGG Cuts at: 1108 3192 3250 3524 3562 1108 Size: 2084 58 274 38 3961 Fragments arranged by size: 3961 2084 274 58 38 PmeI GTTT'AAAC Cuts at: 3719 3719 Size: 6415 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4484 4516 5997 6029 4484 Size: 32 1481 32 4870 Fragments arranged by size: 4870 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6415 PsiI TTA'TAA Cuts at: 3985 3985 Size: 6415 PspOMI G'GGCC_C Cuts at: 2016 2016 Size: 6415 PstI C_TGCA'G Cuts at: 830 2040 2993 3422 3461 3663 3767 830 Size: 1210 953 429 39 202 104 3478 Fragments arranged by size: 3478 1210 953 429 202 104 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6415 SacI G_AGCT'C Cuts at: 2237 2237 Size: 6415 SalI G'TCGA_C Cuts at: 1936 3757 1936 Size: 1821 4594 SapI GCTCTTCn'nnn_ Cuts at: 2625 2625 Size: 6415 SbfI CC_TGCA'GG Cuts at: 3422 3767 3422 Size: 345 6070 ScaI AGT'ACT Cuts at: 4663 6375 4663 Size: 1712 4703 SexAI A'CCwGG_T Cuts at: 2797 2797 Size: 6415 SfcI C'TryA_G Cuts at: 826 1045 2036 2260 2472 2989 3006 3418 3457 Size: 219 991 224 212 517 17 412 39 Cuts at: 3457 3659 3763 4898 5579 5770 826 Size: 202 104 1135 681 191 1471 Fragments arranged by size: 1471 1135 991 681 517 412 224 219 212 202 191 104 39 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6415 SmaI CCC'GGG Cuts at: 1872 2021 2130 3742 1872 Size: 149 109 1612 4545 Fragments arranged by size: 4545 1612 149 109 SmlI C'TyrA_G Cuts at: 820 1017 1943 3451 4519 5420 5682 820 Size: 197 926 1508 1068 901 262 1553 Fragments arranged by size: 1553 1508 1068 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4206 493 Size: 3713 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6415 SphI G_CATG'C Cuts at: 1132 3279 3773 1132 Size: 2147 494 3774 Fragments arranged by size: 3774 2147 494 SspI AAT'ATT Cuts at: 5 52 2762 4339 5 Size: 47 2710 1577 2081 Fragments arranged by size: 2710 2081 1577 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6415 StyI C'CwwG_G Cuts at: 513 1065 2004 2118 2593 2907 3060 3135 3191 Size: 552 939 114 475 314 153 75 56 Cuts at: 3191 3492 3570 3854 513 Size: 301 78 284 3074 Fragments arranged by size: 3074 939 552 475 314 301 284 153 114 78 75 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2126 2900 4442 4459 4612 4797 5242 Size: 1255 546 774 1542 17 153 185 445 Cuts at: 5242 325 Size: 1498 Fragments arranged by size: 1542 1498 1255 774 546 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2550 4661 6373 Size: 274 80 33 51 1443 573 2111 1712 Cuts at: 6373 96 Size: 138 Fragments arranged by size: 2111 1712 1443 573 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3489 3521 6375 6407 3489 Size: 32 2854 32 3497 Fragments arranged by size: 3497 2854 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3020 1316 Size: 1704 4711 XbaI T'CTAG_A Cuts at: 3751 3751 Size: 6415 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3067 3067 Size: 6415 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6415 XmaI C'CCGG_G Cuts at: 1870 2019 2128 3740 1870 Size: 149 109 1612 4545 Fragments arranged by size: 4545 1612 149 109 XmnI GAAnn'nnTTC Cuts at: 4544 4544 Size: 6415 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2149 4223 276 Size: 53 83 186 936 615 2074 2468 Fragments arranged by size: 2468 2074 936 615 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI