(Circular) (Six-base) MAPSORT of: pFN21AB5983.seq Check: 9364 from: 1 to: 6268 pFN21AB5983 6268 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4078 278 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3611 1937 Size: 1674 4594 Acc65I G'GTAC_C Cuts at: 3589 3589 Size: 6268 AclI AA'CG_TT Cuts at: 1563 4395 4768 1563 Size: 2832 373 3063 Fragments arranged by size: 3063 2832 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2379 2484 2556 2564 3455 4331 5715 5983 Size: 747 105 72 8 891 876 1384 268 Cuts at: 5983 1632 Size: 1917 Fragments arranged by size: 1917 1384 891 876 747 268 105 72 8 AfeI AGC'GCT Cuts at: 2312 3100 2312 Size: 788 5480 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6071 AflIII A'CryG_T Cuts at: 1203 5167 6144 1203 Size: 3964 977 1327 Fragments arranged by size: 3964 1327 977 AgeI A'CCGG_T Cuts at: 2990 5099 2990 Size: 2109 4159 AhdI GACnn_n'nnGTC Cuts at: 4997 4997 Size: 6268 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6268 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6268 AlwNI CAG_nnn'CTG Cuts at: 2190 3046 4011 5583 2190 Size: 856 965 1572 2875 Fragments arranged by size: 2875 1572 965 856 ApaLI G'TGCA_C Cuts at: 4325 5481 4325 Size: 1156 5112 ApoI r'AATT_y Cuts at: 1493 2027 2033 2870 3577 3803 3955 6064 1493 Size: 534 6 837 707 226 152 2109 1697 Fragments arranged by size: 2109 1697 837 707 534 226 152 6 AseI AT'TA_AT Cuts at: 160 1032 4822 160 Size: 872 3790 1606 Fragments arranged by size: 3790 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 AvaI C'yCGr_G Cuts at: 1870 1943 3555 3593 1870 Size: 73 1612 38 4545 Fragments arranged by size: 4545 1612 73 38 BamHI G'GATC_C Cuts at: 3598 3965 3598 Size: 367 5901 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3589 5044 618 Size: 325 217 627 1802 1455 1842 Fragments arranged by size: 1842 1802 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3587 1391 Size: 2196 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3182 928 Size: 971 1283 4014 Fragments arranged by size: 4014 1283 971 BbvCI CC'TCA_GC Cuts at: 3108 3108 Size: 6268 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4459 4493 1903 Size: 34 2522 34 3678 Fragments arranged by size: 3678 2522 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4160 5370 4160 Size: 1210 5058 BclI T'GATC_A Cuts at: 1548 1548 Size: 6268 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3530 4879 136 Size: 107 122 71 631 605 1858 1349 1525 Fragments arranged by size: 1858 1525 1349 631 605 122 107 71 BglII A'GATC_T Cuts at: 6263 6263 Size: 6268 BlpI GC'TnA_GC Cuts at: 3024 3685 3024 Size: 661 5607 Bme1580I G_kGCm'C Cuts at: 1790 3364 4329 5485 1790 Size: 1574 965 1156 2573 Fragments arranged by size: 2573 1574 1156 965 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6268 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2928 3999 4957 Size: 416 53 83 399 24 1511 1071 958 Cuts at: 4957 442 Size: 1753 Fragments arranged by size: 1753 1511 1071 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6268 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2944 3307 2944 Size: 363 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2576 4928 1239 Size: 1337 2352 2579 Fragments arranged by size: 2579 2352 1337 Bpu10I CC'TnA_GC Cuts at: 3108 3108 Size: 6268 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4393 5258 5556 4393 Size: 865 298 5105 Fragments arranged by size: 5105 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4931 882 Size: 617 3432 2219 Fragments arranged by size: 3432 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4059 493 Size: 711 2855 2702 Fragments arranged by size: 2855 2702 711 BsaBI GATnn'nnATC Cuts at: 2238 6262 2238 Size: 4024 2244 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4075 4457 5092 275 Size: 53 83 186 936 2542 382 635 1451 Fragments arranged by size: 2542 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2990 4701 5099 5373 5520 5931 2990 Size: 1711 398 274 147 411 3327 Fragments arranged by size: 3327 1711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6043 Fragments arranged by size: 6043 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2302 1186 Size: 1116 5152 BseYI C'CCAG_C Cuts at: 2190 2740 5471 5967 2190 Size: 550 2731 496 2491 Fragments arranged by size: 2731 2491 550 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3146 1163 Size: 573 1410 4285 Fragments arranged by size: 4285 1410 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3718 4479 5507 1540 Size: 275 186 1717 761 1028 2301 Fragments arranged by size: 2301 1717 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2294 4329 4414 5485 2294 Size: 2035 85 1071 3077 Fragments arranged by size: 3077 2035 1071 85 BsmI GAATG_Cn' Cuts at: 3779 3872 3779 Size: 93 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2294 3364 3587 4329 4414 5485 1391 Size: 399 504 1070 223 742 85 1071 2174 Fragments arranged by size: 2174 1071 1070 742 504 399 223 85 BspEI T'CCGG_A Cuts at: 5931 5931 Size: 6268 BspHI T'CATG_A Cuts at: 4157 5887 4157 Size: 1730 4538 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2307 3623 844 Size: 1463 1316 3489 Fragments arranged by size: 3489 1463 1316 BsrBI CCG'CTC Cuts at: 1394 1823 4155 1394 Size: 429 2332 3507 Fragments arranged by size: 3507 2332 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3035 4763 4937 65 Size: 1183 1787 1728 174 1396 Fragments arranged by size: 1787 1728 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2990 3120 4912 5099 1700 Size: 110 1180 130 1792 187 2869 Fragments arranged by size: 2869 1792 1180 187 130 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6268 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6268 BssSI C'ACGA_G Cuts at: 4328 5340 4328 Size: 1012 5256 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6268 BstBI TT'CG_AA Cuts at: 5072 5072 Size: 6268 BstEII G'GTnAC_C Cuts at: 1184 3326 1184 Size: 2142 4126 BstXI CCAn_nnnn'nTGG Cuts at: 2174 2174 Size: 6268 BstYI r'GATC_y Cuts at: 1972 2148 3598 3965 4350 4367 5819 5905 5917 Size: 176 1450 367 385 17 1452 86 12 Cuts at: 5917 6263 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1450 385 367 346 176 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2285 2285 Size: 6268 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5716 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5529 Fragments arranged by size: 5529 683 56 BtsI GCAGTG_nn' Cuts at: 3784 4578 4598 3784 Size: 794 20 5454 Fragments arranged by size: 5454 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6268 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6233 DraI TTT'AAA Cuts at: 2032 3572 2032 Size: 1540 4728 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5275 809 Size: 507 3959 1802 Fragments arranged by size: 3959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3715 4604 6233 Size: 54 1425 50 275 18 1885 889 1629 Cuts at: 6233 8 Size: 43 Fragments arranged by size: 1885 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3715 1537 Size: 275 1903 4090 Fragments arranged by size: 4090 1903 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3161 4198 1353 Size: 1808 1037 3423 Fragments arranged by size: 3423 1808 1037 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2928 3127 4855 5229 5375 387 Size: 1064 138 1339 199 1728 374 146 1280 Fragments arranged by size: 1728 1339 1280 1064 374 199 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 2484 2556 2564 2576 3455 4331 Size: 393 747 105 72 8 12 879 876 Cuts at: 4331 4928 5715 5983 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 879 876 787 747 597 393 268 105 72 12 8 EcoNI CCTnn'n_nnAGG Cuts at: 2765 2765 Size: 6268 EcoO109I rG'GnC_Cy Cuts at: 3286 3286 Size: 6268 EcoRI G'AATT_C Cuts at: 3577 3955 3577 Size: 378 5890 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3433 3433 Size: 6268 FspI TGC'GCA Cuts at: 4774 4774 Size: 6268 HaeII r_GCGC'y Cuts at: 1678 2314 3102 3121 5415 1678 Size: 636 788 19 2294 2531 Fragments arranged by size: 2531 2294 788 636 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2369 2401 2677 2709 4909 Size: 32 135 32 797 32 276 32 2200 Cuts at: 4909 4941 4983 5015 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2200 797 276 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2758 3612 3858 669 Size: 988 281 820 854 246 3079 Fragments arranged by size: 3079 988 854 820 281 246 HindIII A'AGCT_T Cuts at: 748 3299 748 Size: 2551 3717 HpaI GTT'AAC Cuts at: 3858 3858 Size: 6268 KpnI G_GTAC'C Cuts at: 3593 3593 Size: 6268 MfeI C'AATT_G Cuts at: 3867 3867 Size: 6268 MluI A'CGCG_T Cuts at: 6144 6144 Size: 6268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2159 5382 5566 1305 Size: 854 3223 184 2007 Fragments arranged by size: 3223 2007 854 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4446 Fragments arranged by size: 4446 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2172 2726 4226 4585 4744 518 Size: 648 1006 554 1500 359 159 2042 Fragments arranged by size: 2042 1500 1006 648 554 359 159 MspA1I CmG'CkG Cuts at: 1606 2554 3684 4361 5509 5754 1606 Size: 948 1130 677 1148 245 2120 Fragments arranged by size: 2120 1148 1130 948 677 245 NaeI GCC'GGC Cuts at: 3122 3122 Size: 6268 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5716 NdeI CA'TA_TG Cuts at: 387 2833 387 Size: 2446 3822 NgoMIV G'CCGG_C Cuts at: 3120 3120 Size: 6268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6268 NotI GC'GGCC_GC Cuts at: 3715 3715 Size: 6268 NspI r_CATG'y Cuts at: 1132 1229 2717 3148 3626 5171 1132 Size: 97 1488 431 478 1545 2229 Fragments arranged by size: 2229 1545 1488 478 431 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6268 PciI A'CATG_T Cuts at: 5167 5167 Size: 6268 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6268 PfoI T'CCnGG_A Cuts at: 3293 3293 Size: 6268 PmeI GTTT'AAAC Cuts at: 3572 3572 Size: 6268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4337 4369 5850 5882 4337 Size: 32 1481 32 4723 Fragments arranged by size: 4723 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3286 3286 Size: 6268 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6268 PsiI TTA'TAA Cuts at: 3838 3838 Size: 6268 PstI C_TGCA'G Cuts at: 830 2318 3620 830 Size: 1488 1302 3478 Fragments arranged by size: 3478 1488 1302 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6268 PvuII CAG'CTG Cuts at: 2554 2554 Size: 6268 SalI G'TCGA_C Cuts at: 1936 3610 1936 Size: 1674 4594 SapI GCTCTTCn'nnn_ Cuts at: 3161 3161 Size: 6268 SbfI CC_TGCA'GG Cuts at: 3620 3620 Size: 6268 ScaI AGT'ACT Cuts at: 4516 6228 4516 Size: 1712 4556 SfcI C'TryA_G Cuts at: 826 1045 2274 2314 2407 2523 3616 4751 5432 Size: 219 1229 40 93 116 1093 1135 681 Cuts at: 5432 5623 826 Size: 191 1471 Fragments arranged by size: 1471 1229 1135 1093 681 219 191 116 93 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 SgrAI Cr'CCGG_yG Cuts at: 1700 2990 1700 Size: 1290 4978 SmaI CCC'GGG Cuts at: 1872 3557 3595 1872 Size: 1685 38 4545 Fragments arranged by size: 4545 1685 38 SmlI C'TyrA_G Cuts at: 820 1017 1943 4372 5273 5535 820 Size: 197 926 2429 901 262 1553 Fragments arranged by size: 2429 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4059 493 Size: 3566 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6268 SphI G_CATG'C Cuts at: 1132 3148 3626 1132 Size: 2016 478 3774 Fragments arranged by size: 3774 2016 478 SspI AAT'ATT Cuts at: 5 52 4192 5 Size: 47 4140 2081 Fragments arranged by size: 4140 2081 47 StuI AGG'CCT Cuts at: 1517 2349 2689 1517 Size: 832 340 5096 Fragments arranged by size: 5096 832 340 StyI C'CwwG_G Cuts at: 513 1065 2079 3403 3707 513 Size: 552 1014 1324 304 3074 Fragments arranged by size: 3074 1324 1014 552 304 SwaI ATTT'AAAT Cuts at: 2032 2032 Size: 6268 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4295 4312 4465 4650 5095 325 Size: 1255 2715 17 153 185 445 1498 Fragments arranged by size: 2715 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4514 6226 96 Size: 274 80 33 51 1443 2537 1712 138 Fragments arranged by size: 2537 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6228 6260 6228 Size: 32 6236 Tth111I GACn'n_nGTC Cuts at: 1316 2336 2863 1316 Size: 1020 527 4721 Fragments arranged by size: 4721 1020 527 XbaI T'CTAG_A Cuts at: 3604 3604 Size: 6268 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6268 XmaI C'CCGG_G Cuts at: 1870 3555 3593 1870 Size: 1685 38 4545 Fragments arranged by size: 4545 1685 38 XmnI GAAnn'nnTTC Cuts at: 4397 4397 Size: 6268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4076 276 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI XcmI