Restriction map for
pFN21AB8234
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HindIII HpaI KasI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NarI NciI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 596 GGTACC G^GTACC
AcyI 6 GGCGTC G[GA]^CG[CT]C
AflIII 277 ACATGT A^C[GA][CT]GT
AlwI 474 GGATC GGATC(4/5)
Asp718I 596 GGTACC G^GTACC
AspHI 767 GTGCTC G[AT]GC[AT]^C
AvaII 519 GGACC G^G[AT]CC
BanI 596 GGTACC G^G[CT][GA]CC
BanII 160 GAGCCC G[GA]GC[CT]^C
BbsI 273 GAAGAC GAAGAC(2/6)
BbvCI 27 GCTGAGG CCTCAGC(-5/-2)
BbvI 435 GCAGC GCAGC(8/12)
BfaI 719 CTAG C^TAG
BpmI 85 CTCCAG CTGGAG(16/14)
BpuAI 273 GAAGAC GAAGAC(2/6)
BsaHI 6 GGCGTC G[GA]^CG[CT]C
BsaWI 624 ACCGGA [AT]^CCGG[AT]
BsiHKAI 767 GTGCTC G[AT]GC[AT]^C
BsmFI 518 GGGAC GGGAC(10/14)
BspLU11I 277 ACATGT A^CATGT
BsrFI 650 GCCGGC [GA]^CCGG[CT]
BstEII 586 GGTGACC G^GT[ACGT]ACC
BstPI 586 GGTGACC G^GT[ACGT]ACC
BstXI 240 CCACAAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 383 GCGC GCG^C
Cfr10I 650 GCCGGC [GA]^CCGG[CT]
EcoO65I 586 GGTGACC G^GT[ACGT]ACC
EcoT14I 601 CCATGG C^C[AT][AT]GG
HgaI 7 GCGTC GACGC(5/10)
HhaI 383 GCGC GCG^C
Hin1I 6 GGCGTC G[GA]^CG[CT]C
HinP1I 383 GCGC G^CGC
HphI 586 GGTGA GGTGA(8/7)
KpnI 596 GGTACC GGTAC^C
MaeI 719 CTAG C^TAG
MaeII 356 ACGT A^CGT
MaeIII 587 GTGAC ^GT[ACGT]AC
MseI 16 TTAA T^TAA
MslI 241 CACAAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 650 GCCGGC GCC^GGC
NcoI 601 CCATGG C^CATGG
NgoMIV 650 GCCGGC G^CCGGC
PpuMI 518 GGGACCT [GA]G^G[AT]CC[CT]
SfaNI 711 GATGC GCATC(5/9)
StyI 601 CCATGG C^C[AT][AT]GG
TaqI 324 TCGA T^CGA
Tru9I 16 TTAA T^TAA
TseI 435 GCAGC G^C[AT]GC
Tsp45I 587 GTGAC ^GT[GC]AC
Tsp509I 374 AATT ^AATT
TspRI 744 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 324 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AluI 105 AGCT AG^CT
362 AGCT
BmyI 160 GAGCCC G[AGT]GC[ACT]^C
767 GTGCTC
BsaI 481 GGTCTC GGTCTC(1/5)
579 GGTCTC
BseRI 484 CTCCTC GAGGAG(10/8)
738 CTCCTC
Bsp1286I 160 GAGCCC G[AGT]GC[ACT]^C
767 GTGCTC
BsrI 611 ACTGG ACTGG(1/-1)
745 ACTGG
Cac8I 564 GCCAGC GC[ACGT]^[ACGT]GC
650 GCCGGC
DpnII 328 GATC ^GATC
475 GATC
DraII 518 GGGACCT [GA]G^G[ACGT]CC[CT]
756 AGGCCCT
DsaI 514 CCACGG C^C[GA][CT]GG
601 CCATGG
EarI 52 CTCTTC CTCTTC(1/4)
676 GAAGAG
EcoO109I 518 GGGACCT [GA]G^G[ACGT]CC[CT]
756 AGGCCCT
HapII 625 CCGG C^CGG
651 CCGG
HincII 343 GTTGAC GT[CT]^[GA]AC
447 GTTGAC
HindII 343 GTTGAC GT[CT]^[GA]AC
447 GTTGAC
HpaII 625 CCGG C^CGG
651 CCGG
Ksp632I 52 CTCTTC CTCTTC(1/4)
676 GAAGAG
MboI 328 GATC ^GATC
475 GATC
MspA1I 262 CAGCGG C[AC]G^C[GT]G
728 CAGCGG
MspI 625 CCGG C^CGG
651 CCGG
NdeII 328 GATC ^GATC
475 GATC
NspI 277 ACATGT [GA]CATG^[CT]
339 GCATGT
Sau3AI 328 GATC ^GATC
475 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AfaI BsaJI BsiYI BslI
BsmAI BssKI BstF5I BstNI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MboII MnlI MvaI
MwoI NlaIII NlaIV PleI RsaI
Sau96I ScrFI TfiI XcmI