################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrDI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FokI FseI FspI HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AocI 304 CCTCAGG CC^T[ACGT]AGG Aor51HI 197 AGCGCT AGC^GCT AvaII 362 GGTCC G^G[AT]CC BamHI 492 GGATCC G^GATCC BbvCI 475 GCTGAGG CCTCAGC(-5/-2) BpmI 439 CTGGAG CTGGAG(16/14) BsaJI 587 CCAAGG C^C[ACGT][ACGT]GG BsmFI 398 GGGAC GGGAC(10/14) BspMI 569 GCAGGT ACCTGC(4/8) BsrBI 298 GAGCGG CCGCTC(-3/-3) BstBI 149 TTCGAA TT^CGAA Bsu36I 304 CCTCAGG CC^T[ACGT]AGG EarI 146 CTCTTC CTCTTC(1/4) Eco47III 197 AGCGCT AGC^GCT Eco81I 304 CCTCAGG CC^T[ACGT]AGG EcoT14I 587 CCAAGG C^C[AT][AT]GG HaeII 197 AGCGCT [GA]GCGC^[CT] Ksp632I 146 CTCTTC CTCTTC(1/4) MseI 611 TTAA T^TAA NheI 30 GCTAGC G^CTAGC NlaIV 492 GGATCC GG[ACGT]^[ACGT]CC NspV 149 TTCGAA TT^CGAA PleI 87 GACTC GAGTC(4/5) SfuI 149 TTCGAA TT^CGAA SmlI 236 CTCAAG C^T[CT][GA]AG StyI 587 CCAAGG C^C[AT][AT]GG TaqI 150 TCGA T^CGA TfiI 457 GAATC G^A[AT]TC Tru9I 611 TTAA T^TAA Tsp509I 246 AATT ^AATT TthHB8I 150 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Asp700I 121 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 142 GAACCTCTTC AspHI 313 GAGCTC G[AT]GC[AT]^C 341 GAGCTC BanII 313 GAGCTC G[GA]GC[CT]^C 341 GAGCTC BmyI 313 GAGCTC G[AGT]GC[ACT]^C 341 GAGCTC BseRI 325 GAGGAG GAGGAG(10/8) 336 GAGGAG BsiHKAI 313 GAGCTC G[AT]GC[AT]^C 341 GAGCTC BsmAI 85 GAGAC GTCTC(1/5) 328 GAGAC Bsp1286I 313 GAGCTC G[AGT]GC[ACT]^C 341 GAGCTC Bsp1407I 368 TGTACA T^GTACA 593 TGTACA BsrGI 368 TGTACA T^GTACA 593 TGTACA BsrI 222 ACTGG ACTGG(1/-1) 453 ACTGG CfoI 39 GCGC GCG^C 198 GCGC Cfr13I 362 GGTCC G^G[ACGT]CC 523 GGGCC FauI 50 GCGGG CCCGC(4/6) 489 GCGGG HgaI 72 GACGC GACGC(5/10) 100 GCGTC HhaI 39 GCGC GCG^C 198 GCGC HinP1I 39 GCGC G^CGC 198 GCGC HinfI 87 GACTC G^A[ACGT]TC 457 GAATC NlaIII 132 CATG CATG^ 472 CATG SacI 313 GAGCTC GAGCT^C 341 GAGCTC Sau96I 362 GGTCC G^G[ACGT]CC 523 GGGCC SspBI 368 TGTACA T^GTACA 593 TGTACA XmnI 121 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 142 GAACCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BfaI BsiYI BslI BstYI Cac8I DdeI DpnII Fnu4HI HaeIII HphI ItaI MaeI MboI MboII MflI MnlI MslI MwoI NdeII RsaI Sau3AI TseI XhoII