Restriction map for
pFN21AB6843
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiHKAI BsiWI
BsiYI BslI BsmBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MboII
MfeI MluI MluNI MroI MscI
MseI MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AlwNI 439 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 465 CCTCAGG CC^T[ACGT]AGG
AvaI 64 CCCGAG C^[CT]CG[GA]G
AviII 218 TGCGCA TGC^GCA
BanI 129 GGCACC G^G[CT][GA]CC
BsiEI 11 CGGTCG CG[GA][CT]^CG
BsmI 317 GAATGC GAATGC(1/-1)
BsoBI 64 CCCGAG C^[CT]CG[GA]G
BstAPI 447 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 75 CATCC GGATG(2/0)
BstXI 215 CCATGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 326 AGATCC [GA]^GATC[CT]
Bsu36I 465 CCTCAGG CC^T[ACGT]AGG
Cac8I 484 GCAAGC GC[ACGT]^[ACGT]GC
DdeI 466 CTCAG C^T[ACGT]AG
DraII 461 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 33 TGGCCG [CT]^GGCC[GA]
Eco81I 465 CCTCAGG CC^T[ACGT]AGG
EcoO109I 461 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 71 CCCGC CCCGC(4/6)
FokI 75 CATCC GGATG(9/13)
FspI 218 TGCGCA TGC^GCA
HinfI 67 GAGTC G^A[ACGT]TC
MflI 326 AGATCC [GA]^GATC[CT]
MspA1I 439 CAGCTG C[AC]G^C[GT]G
PleI 67 GAGTC GAGTC(4/5)
PpuMI 461 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 439 CAGCTG CAG^CTG
ScaI 273 AGTACT AGT^ACT
Tsp509I 382 AATT ^AATT
TspRI 362 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 326 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 274 GTAC GT^AC
288 GTAC
AvaII 61 GGTCC G^G[AT]CC
462 GGACC
BglI 47 GCCGTATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
352 GCCAAGATGGC
BsmAI 197 GAGAC GTCTC(1/5)
413 GAGAC
BsmFI 62 GTCCC GGGAC(10/14)
69 GTCCC
BsrI 276 ACTGG ACTGG(1/-1)
301 ACTGG
BssKI 477 CCAGG ^CC[ACGT]GG
517 CCAGG
BstNI 477 CCAGG CC^[AT]GG
517 CCAGG
CfoI 139 GCGC GCG^C
219 GCGC
DsaI 30 CCATGG C^C[GA][CT]GG
133 CCATGG
EcoRII 477 CCAGG ^CC[AT]GG
517 CCAGG
EcoT14I 30 CCATGG C^C[AT][AT]GG
133 CCATGG
HhaI 139 GCGC GCG^C
219 GCGC
HinP1I 139 GCGC G^CGC
219 GCGC
MslI 216 CATGCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
312 CACAAGAATG
MvaI 477 CCAGG CC^[AT]GG
517 CCAGG
NcoI 30 CCATGG C^CATGG
133 CCATGG
RsaI 274 GTAC GT^AC
288 GTAC
ScrFI 477 CCAGG CC^[ACGT]GG
517 CCAGG
SfaNI 74 GCATC GCATC(5/9)
122 GCATC
StyI 30 CCATGG C^C[AT][AT]GG
133 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BsaJI BstUI Cfr13I DpnII Fnu4HI
HaeIII ItaI MboI MnlI MvnI
MwoI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I SfcI TaqI TseI
TthHB8I