################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII AccI AccIII AclI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DdeI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HindIII HpaI KasI KspI MaeII MfeI MluI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 59 CGCG CG^CG AcsI 682 AAATTT [GA]^AATT[CT] AcyI 269 GACGCC G[GA]^CG[CT]C AflII 871 CTTAAG C^TTAAG ApoI 682 AAATTT [GA]^AATT[CT] AvaI 29 CCCGGG C^[CT]CG[GA]G BalI 71 TGGCCA TGG^CCA BbsI 113 GAAGAC GAAGAC(2/6) BbvI 493 GCTGC GCAGC(8/12) BfrI 871 CTTAAG C^TTAAG BpmI 809 CTCCAG CTGGAG(16/14) BpuAI 113 GAAGAC GAAGAC(2/6) BsaBI 859 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 269 GACGCC G[GA]^CG[CT]C BsaWI 105 ACCGGA [AT]^CCGG[AT] BseRI 424 GAGGAG GAGGAG(10/8) BsmAI 290 GTCTC GTCTC(1/5) BsmFI 8 GTCCC GGGAC(10/14) BsmI 608 GAATGC GAATGC(1/-1) BsoBI 29 CCCGGG C^[CT]CG[GA]G BsrFI 4 GCCGGT [GA]^CCGG[CT] BstUI 59 CGCG CG^CG Cac8I 53 GCCTGC GC[ACGT]^[ACGT]GC Cfr10I 4 GCCGGT [GA]^CCGG[CT] DrdI 116 GACCAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 657 CCGTGG C^C[GA][CT]GG EcoRV 736 GATATC GAT^ATC EcoT14I 784 CCAAGG C^C[AT][AT]GG FauI 188 CCCGC CCCGC(4/6) HgaI 269 GACGC GACGC(5/10) Hin1I 269 GACGCC G[GA]^CG[CT]C HincII 338 GTCAAC GT[CT]^[GA]AC HindII 338 GTCAAC GT[CT]^[GA]AC HphI 822 GGTGA GGTGA(8/7) MamI 859 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 71 TGGCCA TGG^CCA MscI 71 TGGCCA TGG^CCA MspA1I 415 CAGCTG C[AC]G^C[GT]G MvnI 59 CGCG CG^CG PstI 301 CTGCAG CTGCA^G PvuII 415 CAGCTG CAG^CTG SfaNI 646 GATGC GCATC(5/9) SmaI 29 CCCGGG CCC^GGG SmlI 871 CTTAAG C^T[CT][GA]AG SspI 457 AATATT AAT^ATT StyI 784 CCAAGG C^C[AT][AT]GG TseI 493 GCTGC G^C[AT]GC Tsp45I 379 GTGAC ^GT[GC]AC Tsp509I 683 AATT ^AATT TspRI 642 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 357 CCAGCTTGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 29 CCCGGG C^CCGGG XmaI 29 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 630 GGTACC G^GTACC 662 GGTACC ApaI 26 GGGCCC GGGCC^C 32 GGGCCC Asp718I 630 GGTACC G^GTACC 662 GGTACC AvaII 7 GGTCC G^G[AT]CC 39 GGACC BamHI 672 GGATCC G^GATCC 780 GGATCC BanI 630 GGTACC G^G[CT][GA]CC 662 GGTACC BanII 26 GGGCCC G[GA]GC[CT]^C 32 GGGCCC BmyI 26 GGGCCC G[AGT]GC[ACT]^C 32 GGGCCC Bsp1286I 26 GGGCCC G[AGT]GC[ACT]^C 32 GGGCCC BsrI 627 ACTGG ACTGG(1/-1) 273 CCAGT BstYI 672 GGATCC [GA]^GATC[CT] 780 GGATCC EarI 754 CTCTTC CTCTTC(1/4) 131 GAAGAG Fnu4HI 57 GCCGC GC^[ACGT]GC 493 GCTGC ItaI 57 GCCGC GC^[ACGT]GC 493 GCTGC KpnI 630 GGTACC GGTAC^C 662 GGTACC Ksp632I 754 CTCTTC CTCTTC(1/4) 131 GAAGAG MaeIII 379 GTGAC ^GT[ACGT]AC 520 GTTAC MflI 672 GGATCC [GA]^GATC[CT] 780 GGATCC MwoI 487 GCTATTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 670 GCGGATCCTGC PleI 213 GACTC GAGTC(4/5) 288 GAGTC TfiI 87 GAATC G^A[AT]TC 230 GAATC XhoII 672 GGATCC [GA]^GATC[CT] 780 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI AluI AlwI BcnI BfaI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DpnII DraII EaeI EcoO109I EcoRII FokI HaeIII HapII HinfI HpaII MaeI MboI MboII MnlI MseI MspI MvaI NciI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI Tru9I