(Circular) (Six-base) MAPSORT of: pFN21AE0146.seq Check: 4707 from: 1 to: 5593 pFN21AE0146 5593 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3403 278 Size: 53 83 186 936 1867 2468 Fragments arranged by size: 2468 1867 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2936 1937 Size: 999 4594 Acc65I G'GTAC_C Cuts at: 2635 2667 2914 2635 Size: 32 247 5314 Fragments arranged by size: 5314 247 32 AclI AA'CG_TT Cuts at: 1563 3720 4093 1563 Size: 2157 373 3063 Fragments arranged by size: 3063 2157 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2466 2715 3656 5040 5308 1632 Size: 834 249 941 1384 268 1917 Fragments arranged by size: 1917 1384 941 834 268 249 AflII C'TTAA_G Cuts at: 820 1017 2876 820 Size: 197 1859 3537 Fragments arranged by size: 3537 1859 197 AflIII A'CryG_T Cuts at: 1203 4492 5469 1203 Size: 3289 977 1327 Fragments arranged by size: 3289 1327 977 AgeI A'CCGG_T Cuts at: 4424 4424 Size: 5593 AhdI GACnn_n'nnGTC Cuts at: 4322 4322 Size: 5593 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5593 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5593 AlwNI CAG_nnn'CTG Cuts at: 3336 4908 3336 Size: 1572 4021 ApaI G_GGCC'C Cuts at: 2035 2041 2035 Size: 6 5587 ApaLI G'TGCA_C Cuts at: 3650 4806 3650 Size: 1156 4437 ApoI r'AATT_y Cuts at: 1493 2687 2902 3128 3280 5389 1493 Size: 1194 215 226 152 2109 1697 Fragments arranged by size: 2109 1697 1194 226 215 152 AseI AT'TA_AT Cuts at: 160 1032 4147 160 Size: 872 3115 1606 Fragments arranged by size: 3115 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2918 1870 Size: 73 91 884 4545 Fragments arranged by size: 4545 884 91 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2294 2327 2294 Size: 33 5560 BamHI G'GATC_C Cuts at: 2677 2785 2923 3290 2677 Size: 108 138 367 4980 Fragments arranged by size: 4980 367 138 108 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2635 2667 2914 4369 618 Size: 325 217 627 848 32 247 1455 1842 Fragments arranged by size: 1842 1455 848 627 325 247 217 32 BanII G_rGCy'C Cuts at: 1391 2035 2041 2912 1391 Size: 644 6 871 4072 Fragments arranged by size: 4072 871 644 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2125 928 Size: 971 226 4396 Fragments arranged by size: 4396 971 226 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3784 3818 1903 Size: 34 1847 34 3678 Fragments arranged by size: 3678 1847 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3485 4695 3485 Size: 1210 4383 BclI T'GATC_A Cuts at: 1548 1548 Size: 5593 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4204 136 Size: 107 122 71 631 605 2532 1525 Fragments arranged by size: 2532 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5588 5588 Size: 5593 BlpI GC'TnA_GC Cuts at: 3010 3010 Size: 5593 Bme1580I G_kGCm'C Cuts at: 1790 2035 2041 3654 4810 1790 Size: 245 6 1613 1156 2573 Fragments arranged by size: 2573 1613 1156 245 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5593 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3324 4282 442 Size: 416 53 83 399 24 1907 958 1753 Fragments arranged by size: 1907 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5593 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2799 4253 1239 Size: 1560 1454 2579 Fragments arranged by size: 2579 1560 1454 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3718 4583 4881 3718 Size: 865 298 4430 Fragments arranged by size: 4430 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4256 882 Size: 617 2757 2219 Fragments arranged by size: 2757 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3384 493 Size: 711 2180 2702 Fragments arranged by size: 2702 2180 711 BsaBI GATnn'nnATC Cuts at: 2868 5587 2868 Size: 2719 2874 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2275 3400 3782 4417 Size: 53 83 186 936 742 1125 382 635 Cuts at: 4417 275 Size: 1451 Fragments arranged by size: 1451 1125 936 742 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2110 4026 4424 4698 4845 5256 2110 Size: 1916 398 274 147 411 2447 Fragments arranged by size: 2447 1916 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2125 2155 1178 Size: 30 165 30 722 30 4616 Fragments arranged by size: 4616 722 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 1186 Size: 1258 4335 BseYI C'CCAG_C Cuts at: 2361 4796 5292 2361 Size: 2435 496 2662 Fragments arranged by size: 2662 2435 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5020 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3043 3804 4832 1540 Size: 275 186 1042 761 1028 2301 Fragments arranged by size: 2301 1042 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3654 3739 4810 3654 Size: 85 1071 4437 Fragments arranged by size: 4437 1071 85 BsmI GAATG_Cn' Cuts at: 2619 3104 3197 2619 Size: 485 93 5015 Fragments arranged by size: 5015 485 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2041 2912 3654 3739 4810 1391 Size: 399 245 6 871 742 85 1071 2174 Fragments arranged by size: 2174 1071 871 742 399 245 85 6 BspEI T'CCGG_A Cuts at: 5256 5256 Size: 5593 BspHI T'CATG_A Cuts at: 3482 5212 3482 Size: 1730 3863 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2948 844 Size: 2104 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3480 1394 Size: 429 1657 3507 Fragments arranged by size: 3507 1657 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4088 4262 65 Size: 1183 2840 174 1396 Fragments arranged by size: 2840 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 4237 4424 1700 Size: 110 199 2228 187 2869 Fragments arranged by size: 2869 2228 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5593 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5593 BssSI C'ACGA_G Cuts at: 3653 4665 3653 Size: 1012 4581 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5593 BstBI TT'CG_AA Cuts at: 4397 4397 Size: 5593 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5593 BstYI r'GATC_y Cuts at: 1972 2677 2785 2923 3290 3675 3692 5144 5230 Size: 705 108 138 367 385 17 1452 86 Cuts at: 5230 5242 5588 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 705 385 367 346 138 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2662 513 Size: 552 939 658 3444 Fragments arranged by size: 3444 939 658 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4854 Fragments arranged by size: 4854 683 56 BtsI GCAGTG_nn' Cuts at: 3109 3903 3923 3109 Size: 794 20 4779 Fragments arranged by size: 4779 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5593 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5558 DraI TTT'AAA Cuts at: 2897 2897 Size: 5593 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2127 4600 809 Size: 507 811 2473 1802 Fragments arranged by size: 2473 1802 811 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2076 2835 Size: 54 1425 50 275 18 177 69 759 Cuts at: 2835 3040 3929 5558 8 Size: 205 889 1629 43 Fragments arranged by size: 1629 1425 889 759 275 205 177 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3040 1537 Size: 275 1228 4090 Fragments arranged by size: 4090 1228 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2131 2765 3523 1353 Size: 778 634 758 3423 Fragments arranged by size: 3423 778 758 634 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2690 4180 4554 4700 387 Size: 1064 138 1101 1490 374 146 1280 Fragments arranged by size: 1490 1280 1101 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2466 2715 2799 3656 4253 5040 5308 Size: 393 834 249 84 857 597 787 268 Cuts at: 5308 1239 Size: 1524 Fragments arranged by size: 1524 857 834 787 597 393 268 249 84 EcoO109I rG'GnC_Cy Cuts at: 2031 2038 2056 2031 Size: 7 18 5568 Fragments arranged by size: 5568 18 7 EcoRI G'AATT_C Cuts at: 2902 3280 2902 Size: 378 5215 EcoRV GAT'ATC Cuts at: 2743 2743 Size: 5593 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2720 2720 Size: 5593 FspI TGC'GCA Cuts at: 4099 4099 Size: 5593 HaeII r_GCGC'y Cuts at: 1678 4740 1678 Size: 3062 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2694 2726 4234 4266 4308 Size: 32 135 32 1122 32 1508 32 42 Cuts at: 4308 4340 1373 Size: 32 2626 Fragments arranged by size: 2626 1508 1122 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2345 2937 3183 669 Size: 988 281 407 592 246 3079 Fragments arranged by size: 3079 988 592 407 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5593 HpaI GTT'AAC Cuts at: 3183 3183 Size: 5593 KpnI G_GTAC'C Cuts at: 2639 2671 2918 2639 Size: 32 247 5314 Fragments arranged by size: 5314 247 32 MfeI C'AATT_G Cuts at: 3192 3192 Size: 5593 MluI A'CGCG_T Cuts at: 5469 5469 Size: 5593 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2111 4707 4891 1305 Size: 806 2596 184 2007 Fragments arranged by size: 2596 2007 806 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2078 10 Size: 54 1425 343 246 3525 Fragments arranged by size: 3525 1425 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 3551 3910 4069 518 Size: 648 2385 359 159 2042 Fragments arranged by size: 2385 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2422 3009 3686 4834 5079 1606 Size: 816 587 677 1148 245 2120 Fragments arranged by size: 2120 1148 816 677 587 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4102 Fragments arranged by size: 4102 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5593 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5593 NotI GC'GGCC_GC Cuts at: 3040 3040 Size: 5593 NspI r_CATG'y Cuts at: 1132 1229 2951 4496 1132 Size: 97 1722 1545 2229 Fragments arranged by size: 2229 1722 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5593 PciI A'CATG_T Cuts at: 4492 4492 Size: 5593 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5593 PfoI T'CCnGG_A Cuts at: 2815 2815 Size: 5593 PmeI GTTT'AAAC Cuts at: 2897 2897 Size: 5593 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3662 3694 5175 5207 3662 Size: 32 1481 32 4048 Fragments arranged by size: 4048 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5593 PsiI TTA'TAA Cuts at: 3163 3163 Size: 5593 PspOMI G'GGCC_C Cuts at: 2031 2037 2031 Size: 6 5587 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2651 2683 2651 Size: 32 5561 PstI C_TGCA'G Cuts at: 830 2310 2945 830 Size: 1480 635 3478 Fragments arranged by size: 3478 1480 635 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5593 PvuII CAG'CTG Cuts at: 2422 2422 Size: 5593 SalI G'TCGA_C Cuts at: 1936 2935 1936 Size: 999 4594 SbfI CC_TGCA'GG Cuts at: 2945 2945 Size: 5593 ScaI AGT'ACT Cuts at: 3841 5553 3841 Size: 1712 3881 SfcI C'TryA_G Cuts at: 826 1045 2306 2529 2859 2941 4076 4757 4948 Size: 219 1261 223 330 82 1135 681 191 Cuts at: 4948 826 Size: 1471 Fragments arranged by size: 1471 1261 1135 681 330 223 219 191 82 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5593 SmaI CCC'GGG Cuts at: 1872 2036 2920 1872 Size: 164 884 4545 Fragments arranged by size: 4545 884 164 SmlI C'TyrA_G Cuts at: 820 1017 1943 2876 3697 4598 4860 820 Size: 197 926 933 821 901 262 1553 Fragments arranged by size: 1553 933 926 901 821 262 197 SnaBI TAC'GTA Cuts at: 493 3384 493 Size: 2891 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5593 SphI G_CATG'C Cuts at: 1132 2951 1132 Size: 1819 3774 SrfI GCCC'GGGC Cuts at: 2036 2036 Size: 5593 SspI AAT'ATT Cuts at: 5 52 2464 3517 5 Size: 47 2412 1053 2081 Fragments arranged by size: 2412 2081 1053 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5593 StyI C'CwwG_G Cuts at: 513 1065 2004 2789 3032 513 Size: 552 939 785 243 3074 Fragments arranged by size: 3074 939 785 552 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2812 3620 3637 3790 3975 4420 325 Size: 1255 1232 808 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1232 808 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2470 2774 3839 Size: 274 80 33 51 1443 493 304 1065 Cuts at: 3839 5551 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1065 493 304 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2811 2843 5553 5585 2811 Size: 32 2710 32 2819 Fragments arranged by size: 2819 2710 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5593 XbaI T'CTAG_A Cuts at: 2929 2929 Size: 5593 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2369 2369 Size: 5593 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5593 XmaI C'CCGG_G Cuts at: 1870 2034 2918 1870 Size: 164 884 4545 Fragments arranged by size: 4545 884 164 XmnI GAAnn'nnTTC Cuts at: 3722 3722 Size: 5593 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3401 276 Size: 53 83 186 936 1867 2468 Fragments arranged by size: 2468 1867 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI