Restriction map for
pFN21AE0552
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AviII AvrII BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BseRI
BsiEI BsiHKAI Bsp1407I BspDI BspHI
BspMI BsrDI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MfeI MluI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 643 GTAGAC GT^[AC][GT]AC
AccIII 539 TCCGGA T^CCGGA
AflIII 765 ACATGT A^C[GA][CT]GT
AlwNI 481 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 312 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 685 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 774 TGGCCA TGG^CCA
BanII 295 GGGCTC G[GA]GC[CT]^C
BbrPI 19 CACGTG CAC^GTG
BmyI 295 GGGCTC G[AGT]GC[ACT]^C
BsaAI 19 CACGTG [CT]AC^GT[GA]
BsaBI 357 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 539 TCCGGA [AT]^CCGG[AT]
BseAI 539 TCCGGA T^CCGGA
BsiWI 251 CGTACG C^GTACG
BsmAI 211 GTCTC GTCTC(1/5)
BsmBI 210 CGTCTC CGTCTC(1/5)
Bsp1286I 295 GGGCTC G[AGT]GC[ACT]^C
BspEI 539 TCCGGA T^CCGGA
BspLU11I 765 ACATGT A^CATGT
BsrBI 29 CCGCTC CCGCTC(-3/-3)
BsrFI 50 GCCGGC [GA]^CCGG[CT]
BssSI 376 CACGAG CACGAG(-5/-1)
BstAPI 480 GCAGTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 522 GGTCACC G^GT[ACGT]ACC
BstPI 522 GGTCACC G^GT[ACGT]ACC
BstYI 542 GGATCT [GA]^GATC[CT]
CfoI 747 GCGC GCG^C
Cfr10I 50 GCCGGC [GA]^CCGG[CT]
DdeI 287 CTGAG C^T[ACGT]AG
DraII 689 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 514 GACTCGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 689 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 522 GGTCACC G^GT[ACGT]ACC
HhaI 747 GCGC GCG^C
HinP1I 747 GCGC G^CGC
HinfI 514 GACTC G^A[ACGT]TC
KspI 757 CCGCGG CCGC^GG
MamI 357 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 542 GGATCT [GA]^GATC[CT]
MluNI 774 TGGCCA TGG^CCA
MroI 539 TCCGGA T^CCGGA
MscI 774 TGGCCA TGG^CCA
NaeI 50 GCCGGC GCC^GGC
NgoMIV 50 GCCGGC G^CCGGC
PaeR7I 225 CTCGAG C^TCGAG
PflFI 685 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 18 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 514 GACTC GAGTC(4/5)
PmaCI 19 CACGTG CAC^GTG
PmlI 19 CACGTG CAC^GTG
PpuMI 689 GGGTCCC [GA]G^G[AT]CC[CT]
SacII 757 CCGCGG CCGC^GG
SmlI 225 CTCGAG C^T[CT][GA]AG
StuI 95 AGGCCT AGG^CCT
Tsp45I 523 GTCAC ^GT[GC]AC
Tth111I 685 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 18 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 225 CTCGAG C^TCGAG
XhoII 542 GGATCT [GA]^GATC[CT]
XmnI 312 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 267 GATCC GGATC(4/5)
542 GGATC
AvaI 225 CTCGAG C^[CT]CG[GA]G
300 CCCGAG
BanI 38 GGCACC G^G[CT][GA]CC
162 GGTGCC
BcnI 159 CCCGG CC^[GC]GG
896 CCCGG
BpmI 185 CTCCAG CTGGAG(16/14)
613 CTGGAG
BsmI 828 GAATGC GAATGC(1/-1)
4 GCATTC
BsoBI 225 CTCGAG C^[CT]CG[GA]G
300 CCCGAG
EcoT14I 556 CCATGG C^C[AT][AT]GG
660 CCATGG
MaeII 20 ACGT A^CGT
260 ACGT
MaeIII 523 GTCAC ^GT[ACGT]AC
762 GTTAC
MboII 312 GAAGA GAAGA(8/7)
699 GAAGA
MspA1I 706 CAGCGG C[AC]G^C[GT]G
757 CCGCGG
NciI 159 CCCGG CC^[GC]GG
896 CCCGG
NcoI 556 CCATGG C^CATGG
660 CCATGG
NspI 141 GCATGT [GA]CATG^[CT]
765 ACATGT
StyI 556 CCATGG C^C[AT][AT]GG
660 CCATGG
XcmI 556 CCATGGCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
783 CCACGAAGGGTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbvI BsaJI BsgI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
BstUI Cac8I Cfr13I DpnII DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HpaII HphI
ItaI MboI MnlI MslI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I