Restriction map for
pFN21AE0552
(Linear) (Six-base) MAPSORT of: KIEE0552.seq Check: 4793 from: 1 to: 906
KIEE0552.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 0 644 906
Size: 644 262
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 493 906
Size: 475 18 413
Fragments arranged by size:
475 413 18
AflIII A'CryG_T
Cuts at: 0 765 906
Size: 765 141
AleI CACnn'nnGTG
Cuts at: 0 823 906
Size: 823 83
AlwNI CAG_nnn'CTG
Cuts at: 0 486 906
Size: 486 420
AvaI C'yCGr_G
Cuts at: 0 225 300 906
Size: 225 75 606
Fragments arranged by size:
606 225 75
BanI G'GyrC_C
Cuts at: 0 38 162 906
Size: 38 124 744
Fragments arranged by size:
744 124 38
BanII G_rGCy'C
Cuts at: 0 299 906
Size: 299 607
BmgBI CAC'GTC
Cuts at: 0 261 906
Size: 261 645
BmrI ACTGGGnnnn_n'
Cuts at: 0 6 682 696 906
Size: 6 676 14 210
Fragments arranged by size:
676 210 14 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 96 906
Size: 96 810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 634 906
Size: 170 464 272
Fragments arranged by size:
464 272 170
BsaAI yAC'GTr
Cuts at: 0 21 906
Size: 21 885
BsaBI GATnn'nnATC
Cuts at: 0 361 906
Size: 361 545
BsaWI w'CCGG_w
Cuts at: 0 539 906
Size: 539 367
BseYI C'CCAG_C
Cuts at: 0 274 291 330 578 906
Size: 274 17 39 248 328
Fragments arranged by size:
328 274 248 39 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 499 760 906
Size: 166 333 261 146
Fragments arranged by size:
333 261 166 146
BsiWI C'GTAC_G
Cuts at: 0 251 906
Size: 251 655
BsmI GAATG_Cn'
Cuts at: 0 4 834 906
Size: 4 830 72
Fragments arranged by size:
830 72 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 216 906
Size: 216 690
Bsp1286I G_dGCh'C
Cuts at: 0 299 906
Size: 299 607
BspEI T'CCGG_A
Cuts at: 0 539 906
Size: 539 367
BsrBI CCG'CTC
Cuts at: 0 31 906
Size: 31 875
BsrFI r'CCGG_y
Cuts at: 0 50 906
Size: 50 856
BssSI C'ACGA_G
Cuts at: 0 376 906
Size: 376 530
BstAPI GCAn_nnn'nTGC
Cuts at: 0 486 906
Size: 486 420
BstEII G'GTnAC_C
Cuts at: 0 522 906
Size: 522 384
BstYI r'GATC_y
Cuts at: 0 542 906
Size: 542 364
BtgI C'CryG_G
Cuts at: 0 556 660 757 906
Size: 556 104 97 149
Fragments arranged by size:
556 149 104 97
DrdI GACnn_nn'nnGTC
Cuts at: 0 520 906
Size: 520 386
EaeI y'GGCC_r
Cuts at: 0 26 404 774 906
Size: 26 378 370 132
Fragments arranged by size:
378 370 132 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 475 493 634 906
Size: 170 305 18 141 272
Fragments arranged by size:
305 272 170 141 18
EcoO109I rG'GnC_Cy
Cuts at: 0 690 906
Size: 690 216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 441 906
Size: 441 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 208 240 906
Size: 15 32 161 32 666
Fragments arranged by size:
666 161 32 32 15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 906
Size: 107 799
MscI TGG'CCA
Cuts at: 0 776 906
Size: 776 130
MslI CAynn'nnrTG
Cuts at: 0 64 244 823 906
Size: 64 180 579 83
Fragments arranged by size:
579 180 83 64
MspA1I CmG'CkG
Cuts at: 0 708 759 906
Size: 708 51 147
Fragments arranged by size:
708 147 51
NaeI GCC'GGC
Cuts at: 0 52 906
Size: 52 854
NcoI C'CATG_G
Cuts at: 0 556 660 906
Size: 556 104 246
Fragments arranged by size:
556 246 104
NgoMIV G'CCGG_C
Cuts at: 0 50 906
Size: 50 856
NspI r_CATG'y
Cuts at: 0 145 769 906
Size: 145 624 137
Fragments arranged by size:
624 145 137
PciI A'CATG_T
Cuts at: 0 765 906
Size: 765 141
PflMI CCAn_nnn'nTGG
Cuts at: 0 24 906
Size: 24 882
PfoI T'CCnGG_A
Cuts at: 0 680 906
Size: 680 226
PmlI CAC'GTG
Cuts at: 0 21 906
Size: 21 885
PpuMI rG'GwC_Cy
Cuts at: 0 690 906
Size: 690 216
PspXI vC'TCGA_Gb
Cuts at: 0 225 906
Size: 225 681
SacII CC_GC'GG
Cuts at: 0 760 906
Size: 760 146
SanDI GG'GwC_CC
Cuts at: 0 690 906
Size: 690 216
SmlI C'TyrA_G
Cuts at: 0 225 906
Size: 225 681
StuI AGG'CCT
Cuts at: 0 97 906
Size: 97 809
StyI C'CwwG_G
Cuts at: 0 556 660 906
Size: 556 104 246
Fragments arranged by size:
556 246 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 106 906
Size: 106 800
TatI w'GTAC_w
Cuts at: 0 74 350 906
Size: 74 276 556
Fragments arranged by size:
556 276 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 662 694 906
Size: 662 32 212
Fragments arranged by size:
662 212 32
Tth111I GACn'n_nGTC
Cuts at: 0 688 906
Size: 688 218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 563 790 906
Size: 563 227 116
Fragments arranged by size:
563 227 116
XhoI C'TCGA_G
Cuts at: 0 225 906
Size: 225 681
XmnI GAAnn'nnTTC
Cuts at: 0 316 906
Size: 316 590
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI AvaI BanI BanII
BmgBI BmrI BplI BpmI BsaAI BsaBI BsaWI BseYI
BsgI BsiWI BsmI BsmBI Bsp1286I BspEI BsrBI BsrFI
BssSI BstAPI BstEII BstYI BtgI DrdI EaeI Eco57MI
EcoO109I FalI Hin4I MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PciI PflMI PfoI PmlI PpuMI
PspXI SacII SanDI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmtI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BsiEI BsiHKAI BspHI
BspMI BsrDI BsrGI BssHII BstBI BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XmaI ZraI