################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AccIII AclI AflIII AgeI Aor51HI AscI AseI AsnI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BlnI BsaAI BsaBI BseAI BsiWI BsmBI BspEI BspHI BspLU11I BssHII BssSI BstBI CpoI DraI DrdI DsaI EagI EclXI Eco47III Eco52I EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NarI NcoI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 1270 GACGTC GACGT^C Acc65I 1593 GGTACC G^GTACC AccI 2661 GTATAC GT^[AC][GT]AC AccII 667 CGCG CG^CG AflII 2527 CTTAAG C^TTAAG AhdI 907 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1081 GTGCAC G^TGCAC ApaLI 1081 GTGCAC G^TGCAC Asp700I 2625 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1593 GGTACC G^GTACC AspEI 907 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1099 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1773 TGGCCA TGG^CCA BbsI 376 GAAGAC GAAGAC(2/6) BciVI 1927 GGATAC GTATCC(6/5) BclI 1740 TGATCA T^GATCA BfrI 2527 CTTAAG C^TTAAG BglI 1979 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 158 AGATCT A^GATCT BpuAI 376 GAAGAC GAAGAC(2/6) BsmI 412 GAATGC GAATGC(1/-1) Bsp1407I 1967 TGTACA T^GTACA BspDI 1762 ATCGAT AT^CGAT BsrDI 531 CATTGC GCAATG(2/0) BsrGI 1967 TGTACA T^GTACA Bst1107I 2661 GTATAC GTA^TAC BstAPI 1851 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 630 GGTGACC G^GT[ACGT]ACC BstPI 630 GGTGACC G^GT[ACGT]ACC BstUI 667 CGCG CG^CG BstZ17I 2661 GTATAC GTA^TAC ClaI 1762 ATCGAT AT^CGAT DraIII 1084 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1773 TGGCCA [CT]^GGCC[GA] Eam1105I 907 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 630 GGTGACC G^GT[ACGT]ACC EcoRI 2431 GAATTC G^AATTC FbaI 1740 TGATCA T^GATCA KpnI 1593 GGTACC GGTAC^C MluNI 1773 TGGCCA TGG^CCA MscI 1773 TGGCCA TGG^CCA MvnI 667 CGCG CG^CG NheI 150 GCTAGC G^CTAGC PflFI 1099 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 2836 GAGCTC GAGCT^C SfaNI 2360 GCATC GCATC(5/9) SmaI 2561 CCCGGG CCC^GGG SspBI 1967 TGTACA T^GTACA StuI 2087 AGGCCT AGG^CCT Tth111I 1099 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 2561 CCCGGG C^CCGGG XmaI 2561 CCCGGG C^CCGGG XmnI 2625 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 2431 GAATTC [GA]^AATT[CT] 2709 GAATTT AcyI 1270 GACGTC G[GA]^CG[CT]C 2314 GGCGTC AlwNI 1186 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG 1505 CAGGGGCTG ApoI 2431 GAATTC [GA]^AATT[CT] 2709 GAATTT BfaI 151 CTAG C^TAG 1547 CTAG BlpI 1110 GCTGAGC GC^T[ACGT]AGC 2733 GCTCAGC Bpu1102I 1110 GCTGAGC GC^T[ACGT]AGC 2733 GCTCAGC BsaHI 1270 GACGTC G[GA]^CG[CT]C 2314 GGCGTC BseRI 2271 CTCCTC GAGGAG(10/8) 102 GAGGAG BsiEI 69 CGACCG CG[GA][CT]^CG 1311 CGACCG BsrBI 1942 GAGCGG CCGCTC(-3/-3) 2782 GAGCGG BstYI 158 AGATCT [GA]^GATC[CT] 2502 GGATCT CelII 1110 GCTGAGC GC^T[ACGT]AGC 2733 GCTCAGC EarI 334 CTCTTC CTCTTC(1/4) 2224 GAAGAG EcoNI 2694 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2772 CCTCCCCCAGG EcoRV 124 GATATC GAT^ATC 892 GATATC EcoT14I 2436 CCAAGG C^C[AT][AT]GG 2567 CCAAGG HaeII 699 GGCGCT [GA]GCGC^[CT] 1226 GGCGCT Hin1I 1270 GACGTC G[GA]^CG[CT]C 2314 GGCGTC Ksp632I 334 CTCTTC CTCTTC(1/4) 2224 GAAGAG MaeI 151 CTAG C^TAG 1547 CTAG MflI 158 AGATCT [GA]^GATC[CT] 2502 GGATCT NaeI 517 GCCGGC GCC^GGC 1708 GCCGGC NgoMIV 517 GCCGGC G^CCGGC 1708 GCCGGC PshAI 45 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1312 GACCGGAGTC SmlI 1300 CTCAAG C^T[CT][GA]AG 2527 CTTAAG StyI 2436 CCAAGG C^C[AT][AT]GG 2567 CCAAGG XhoII 158 AGATCT [GA]^GATC[CT] 2502 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AfaI AluI AlwI AocI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I