(Circular) (Six-base) MAPSORT of: pFN21AE2557.seq Check: 482 from: 1 to: 7546 pFN21AE2557 7546 bp With 182 enzymes: SgfI * December 15, 2014 13:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3445 3445 Size: 7546 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3279 5356 278 Size: 53 83 186 936 1743 2077 2468 Fragments arranged by size: 2468 2077 1743 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4667 4889 1937 Size: 2730 222 4594 Fragments arranged by size: 4594 2730 222 Acc65I G'GTAC_C Cuts at: 3598 4867 3598 Size: 1269 6277 AclI AA'CG_TT Cuts at: 1563 5673 6046 1563 Size: 4110 373 3063 Fragments arranged by size: 4110 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2050 2606 2744 2849 4248 4277 5609 6993 Size: 418 556 138 105 1399 29 1332 1384 Cuts at: 6993 7261 1632 Size: 268 1917 Fragments arranged by size: 1917 1399 1384 1332 556 418 268 138 105 29 AflII C'TTAA_G Cuts at: 820 1017 4532 820 Size: 197 3515 3834 Fragments arranged by size: 3834 3515 197 AflIII A'CryG_T Cuts at: 1203 6445 7422 1203 Size: 5242 977 1327 Fragments arranged by size: 5242 1327 977 AgeI A'CCGG_T Cuts at: 6377 6377 Size: 7546 AhdI GACnn_n'nnGTC Cuts at: 2917 6275 2917 Size: 3358 4188 AleI CACnn'nnGTG Cuts at: 1166 3437 1166 Size: 2271 5275 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2300 4113 1184 Size: 1116 1813 4617 Fragments arranged by size: 4617 1813 1116 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2418 2450 2418 Size: 32 7514 AlwNI CAG_nnn'CTG Cuts at: 3196 3515 5289 6861 3196 Size: 319 1774 1572 3881 Fragments arranged by size: 3881 1774 1572 319 ApaI G_GGCC'C Cuts at: 2229 2411 3986 4761 2229 Size: 182 1575 775 5014 Fragments arranged by size: 5014 1575 775 182 ApaLI G'TGCA_C Cuts at: 3086 5603 6759 3086 Size: 2517 1156 3873 Fragments arranged by size: 3873 2517 1156 ApoI r'AATT_y Cuts at: 1493 4436 4714 4855 5081 5233 7342 1493 Size: 2943 278 141 226 152 2109 1697 Fragments arranged by size: 2943 2109 1697 278 226 152 141 AseI AT'TA_AT Cuts at: 160 1032 6100 160 Size: 872 5068 1606 Fragments arranged by size: 5068 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 AvaI C'yCGr_G Cuts at: 1870 1943 2052 2238 3270 4566 4871 1870 Size: 73 109 186 1032 1296 305 4545 Fragments arranged by size: 4545 1296 1032 305 186 109 73 BamHI G'GATC_C Cuts at: 4876 5243 4876 Size: 367 7179 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2923 3265 3374 3416 3598 Size: 325 217 627 1136 342 109 42 182 Cuts at: 3598 4867 6322 618 Size: 1269 1455 1842 Fragments arranged by size: 1842 1455 1269 1136 627 342 325 217 182 109 42 BanII G_rGCy'C Cuts at: 1391 2229 2411 3986 4307 4761 4845 4865 1391 Size: 838 182 1575 321 454 84 20 4072 Fragments arranged by size: 4072 1575 838 454 321 182 84 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2388 928 Size: 971 489 6086 Fragments arranged by size: 6086 971 489 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5737 5771 1903 Size: 34 50 34 3716 34 3678 Fragments arranged by size: 3716 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3926 5438 6648 3926 Size: 1512 1210 4824 Fragments arranged by size: 4824 1512 1210 BclI T'GATC_A Cuts at: 1548 3745 1548 Size: 2197 5349 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3990 6157 136 Size: 107 122 71 631 605 2318 2167 1525 Fragments arranged by size: 2318 2167 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2163 7541 2163 Size: 5378 2168 BlpI GC'TnA_GC Cuts at: 3116 4739 4963 3116 Size: 1623 224 5699 Fragments arranged by size: 5699 1623 224 Bme1580I G_kGCm'C Cuts at: 1790 2229 2411 2928 3090 3217 3377 3986 4761 Size: 439 182 517 162 127 160 609 775 Cuts at: 4761 5607 6763 1790 Size: 846 1156 2573 Fragments arranged by size: 2573 1156 846 775 609 517 439 182 162 160 127 BmgBI CAC'GTC Cuts at: 1318 2089 1318 Size: 771 6775 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2311 2356 2414 Size: 416 53 83 399 24 894 45 58 Cuts at: 2414 3637 3727 3938 3997 5277 6235 442 Size: 1223 90 211 59 1280 958 1753 Fragments arranged by size: 1753 1280 1223 958 894 416 399 211 90 83 59 58 53 45 24 BmtI G_CTAG'C Cuts at: 1056 2159 1056 Size: 1103 6443 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2711 2837 2894 2936 2993 3003 3239 3347 Size: 1472 126 57 42 57 10 236 108 Cuts at: 3347 3396 3965 4661 6206 1239 Size: 49 569 696 1545 2579 Fragments arranged by size: 2579 1545 1472 696 569 236 126 108 57 57 49 42 10 Bpu10I CC'TnA_GC Cuts at: 2676 3137 4301 2676 Size: 461 1164 5921 Fragments arranged by size: 5921 1164 461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3290 5671 6536 6834 3290 Size: 2381 865 298 4002 Fragments arranged by size: 4002 2381 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2656 3730 3867 4041 6209 882 Size: 617 1157 1074 137 174 2168 2219 Fragments arranged by size: 2219 2168 1157 1074 617 174 137 BsaAI yAC'GTr Cuts at: 493 1204 5337 493 Size: 711 4133 2702 Fragments arranged by size: 4133 2702 711 BsaBI GATnn'nnATC Cuts at: 7540 7540 Size: 7546 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3276 4320 5353 5735 Size: 53 83 186 936 1743 1044 1033 382 Cuts at: 5735 6370 275 Size: 635 1451 Fragments arranged by size: 1743 1451 1044 1033 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2621 3318 4335 5979 6377 6651 6798 7209 2621 Size: 697 1017 1644 398 274 147 411 2958 Fragments arranged by size: 2958 1644 1017 697 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4814 4844 1178 Size: 30 165 30 3411 30 3880 Fragments arranged by size: 3880 3411 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2122 4267 1186 Size: 936 2145 4465 Fragments arranged by size: 4465 2145 936 BseYI C'CCAG_C Cuts at: 2079 2119 2467 2491 2707 2811 3578 3886 4131 Size: 40 348 24 216 104 767 308 245 Cuts at: 4131 6749 7245 2079 Size: 2618 496 2380 Fragments arranged by size: 2618 2380 767 496 348 308 245 216 104 40 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2562 2852 4520 1163 Size: 573 826 290 1668 4189 Fragments arranged by size: 4189 1668 826 573 290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2077 3319 4996 5757 6785 1540 Size: 275 186 76 1242 1677 761 1028 2301 Fragments arranged by size: 2301 1677 1242 1028 761 275 186 76 BsiHKAI G_wGCw'C Cuts at: 2481 3090 4845 5607 5692 6763 2481 Size: 609 1755 762 85 1071 3264 Fragments arranged by size: 3264 1755 1071 762 609 85 BsmI GAATG_Cn' Cuts at: 2423 5057 5150 2423 Size: 2634 93 4819 Fragments arranged by size: 4819 2634 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2411 2481 2928 3090 3217 3377 Size: 399 439 182 70 447 162 127 160 Cuts at: 3377 3986 4307 4761 4845 4865 5607 5692 6763 Size: 609 321 454 84 20 742 85 1071 Cuts at: 6763 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 609 454 447 439 399 321 182 162 160 127 85 84 70 20 BspEI T'CCGG_A Cuts at: 7209 7209 Size: 7546 BspHI T'CATG_A Cuts at: 5435 7165 5435 Size: 1730 5816 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3445 3708 4707 4901 844 Size: 2601 263 999 194 3489 Fragments arranged by size: 3489 2601 999 263 194 BsrBI CCG'CTC Cuts at: 1394 1823 3949 4789 5433 1394 Size: 429 2126 840 644 3507 Fragments arranged by size: 3507 2126 840 644 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2535 6041 6215 65 Size: 1183 1287 3506 174 1396 Fragments arranged by size: 3506 1396 1287 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2522 3419 3713 6190 6377 1700 Size: 110 712 897 294 2477 187 2869 Fragments arranged by size: 2869 2477 897 712 294 187 110 BsrGI T'GTAC_A Cuts at: 96 3972 96 Size: 3876 3670 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7546 BssSI C'ACGA_G Cuts at: 5606 6618 5606 Size: 1012 6534 BstAPI GCAn_nnn'nTGC Cuts at: 794 3862 794 Size: 3068 4478 BstBI TT'CG_AA Cuts at: 6350 6350 Size: 7546 BstEII G'GTnAC_C Cuts at: 1184 2635 1184 Size: 1451 6095 BstXI CCAn_nnnn'nTGG Cuts at: 2455 3923 4051 2455 Size: 1468 128 5950 Fragments arranged by size: 5950 1468 128 BstYI r'GATC_y Cuts at: 1972 2163 4507 4876 5243 5628 5645 7097 7183 Size: 191 2344 369 367 385 17 1452 86 Cuts at: 7183 7195 7541 1972 Size: 12 346 1977 Fragments arranged by size: 2344 1977 1452 385 369 367 346 191 86 17 12 BstZ17I GTA'TAC Cuts at: 4668 4668 Size: 7546 Bsu36I CC'TnA_GG Cuts at: 2294 2735 2787 3603 4753 2294 Size: 441 52 816 1150 5087 Fragments arranged by size: 5087 1150 816 441 52 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6994 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6807 Fragments arranged by size: 6807 683 56 BtsI GCAGTG_nn' Cuts at: 2150 2778 3708 3781 3938 5062 5856 5876 2150 Size: 628 930 73 157 1124 794 20 3820 Fragments arranged by size: 3820 1124 930 794 628 157 73 20 ClaI AT'CG_AT Cuts at: 1554 3768 1554 Size: 2214 5332 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7511 DraI TTT'AAA Cuts at: 4850 4850 Size: 7546 DraIII CAC_nnn'GTG Cuts at: 3094 3094 Size: 7546 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6553 809 Size: 507 5237 1802 Fragments arranged by size: 5237 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3778 4993 5882 Size: 54 1425 50 275 18 1948 1215 889 Cuts at: 5882 7511 8 Size: 1629 43 Fragments arranged by size: 1948 1629 1425 1215 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4993 1537 Size: 275 3181 4090 Fragments arranged by size: 4090 3181 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2345 4224 5476 1353 Size: 992 1879 1252 3423 Fragments arranged by size: 3423 1879 1252 992 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 2325 6133 6507 6653 387 Size: 1064 138 660 76 3808 374 146 1280 Fragments arranged by size: 3808 1280 1064 660 374 146 138 76 EcoICRI GAG'CTC Cuts at: 4843 4843 Size: 7546 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2050 2606 2711 2744 2837 2849 2894 Size: 393 418 556 105 33 93 12 45 Cuts at: 2894 2936 2993 3003 3239 3347 3396 3965 4248 Size: 42 57 10 236 108 49 569 283 Cuts at: 4248 4277 4661 5609 6206 6993 7261 1239 Size: 29 384 948 597 787 268 1524 Fragments arranged by size: 1524 948 787 597 569 556 418 393 384 283 268 236 108 105 93 57 49 45 42 33 29 12 10 EcoNI CCTnn'n_nnAGG Cuts at: 4703 4781 4703 Size: 78 7468 EcoO109I rG'GnC_Cy Cuts at: 2225 2226 2408 2495 2938 3061 3890 3982 3983 Size: 1 182 87 443 123 829 92 1 Cuts at: 3983 4576 4757 4758 4804 2225 Size: 593 181 1 46 4967 Fragments arranged by size: 4967 829 593 443 182 181 123 92 87 46 1 1 1 EcoRI G'AATT_C Cuts at: 4436 4855 5233 4436 Size: 419 378 6749 Fragments arranged by size: 6749 419 378 EcoRV GAT'ATC Cuts at: 2131 2899 2131 Size: 768 6778 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2467 3136 3190 2467 Size: 669 54 6823 Fragments arranged by size: 6823 669 54 FspI TGC'GCA Cuts at: 6052 6052 Size: 7546 HaeII r_GCGC'y Cuts at: 1678 2708 3235 6693 1678 Size: 1030 527 3458 2531 Fragments arranged by size: 3458 2531 1030 527 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2014 2046 2903 2935 6187 Size: 32 135 32 442 32 857 32 3252 Cuts at: 6187 6219 6261 6293 1373 Size: 32 42 32 2626 Fragments arranged by size: 3252 2626 857 442 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4890 5136 669 Size: 988 281 2952 246 3079 Fragments arranged by size: 3079 2952 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7546 HpaI GTT'AAC Cuts at: 5136 5136 Size: 7546 KpnI G_GTAC'C Cuts at: 3602 4871 3602 Size: 1269 6277 MfeI C'AATT_G Cuts at: 5145 5145 Size: 7546 MluI A'CGCG_T Cuts at: 7422 7422 Size: 7546 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3528 6660 6844 1305 Size: 2223 3132 184 2007 Fragments arranged by size: 3132 2223 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3780 10 Size: 54 1425 343 1948 3776 Fragments arranged by size: 3776 1948 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3437 3593 4589 4767 5504 5863 6022 Size: 648 2271 156 996 178 737 359 159 Cuts at: 6022 518 Size: 2042 Fragments arranged by size: 2271 2042 996 737 648 359 178 159 156 MspA1I CmG'CkG Cuts at: 1606 2079 2584 2743 2815 3196 3283 3346 3859 Size: 473 505 159 72 381 87 63 513 Cuts at: 3859 3886 4131 4504 4962 5639 6787 7032 1606 Size: 27 245 373 458 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 513 505 473 458 381 373 245 245 159 87 72 63 27 NaeI GCC'GGC Cuts at: 2524 3715 2524 Size: 1191 6355 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6994 NdeI CA'TA_TG Cuts at: 387 387 Size: 7546 NgoMIV G'CCGG_C Cuts at: 2522 3713 2522 Size: 1191 6355 NheI G'CTAG_C Cuts at: 1052 2155 1052 Size: 1103 6443 NotI GC'GGCC_GC Cuts at: 4993 4993 Size: 7546 NspI r_CATG'y Cuts at: 1132 1229 4904 6449 1132 Size: 97 3675 1545 2229 Fragments arranged by size: 3675 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7546 PciI A'CATG_T Cuts at: 6445 6445 Size: 7546 PflMI CCAn_nnn'nTGG Cuts at: 1108 2934 3487 3787 1108 Size: 1826 553 300 4867 Fragments arranged by size: 4867 1826 553 300 PfoI T'CCnGG_A Cuts at: 2980 3847 4050 4638 2980 Size: 867 203 588 5888 Fragments arranged by size: 5888 867 588 203 PmeI GTTT'AAAC Cuts at: 4850 4850 Size: 7546 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5615 5647 7128 7160 5615 Size: 32 1481 32 6001 Fragments arranged by size: 6001 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2495 2938 3061 2495 Size: 443 123 6980 Fragments arranged by size: 6980 443 123 PshAI GACnn'nnGTC Cuts at: 1613 2054 3321 1613 Size: 441 1267 5838 Fragments arranged by size: 5838 1267 441 PsiI TTA'TAA Cuts at: 5116 5116 Size: 7546 PspOMI G'GGCC_C Cuts at: 2225 2407 3982 4757 2225 Size: 182 1575 775 5014 Fragments arranged by size: 5014 1575 775 182 PstI C_TGCA'G Cuts at: 830 2886 3351 3678 4092 4898 830 Size: 2056 465 327 414 806 3478 Fragments arranged by size: 3478 2056 806 465 414 327 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7546 PvuII CAG'CTG Cuts at: 2584 2743 2815 3346 3859 3886 4504 2584 Size: 159 72 531 513 27 618 5626 Fragments arranged by size: 5626 618 531 513 159 72 27 SacI G_AGCT'C Cuts at: 4845 4845 Size: 7546 SalI G'TCGA_C Cuts at: 1936 4888 1936 Size: 2952 4594 SanDI GG'GwC_CC Cuts at: 2938 2938 Size: 7546 SbfI CC_TGCA'GG Cuts at: 4898 4898 Size: 7546 ScaI AGT'ACT Cuts at: 5794 7506 5794 Size: 1712 5834 SfcI C'TryA_G Cuts at: 826 1045 2882 3347 3674 3810 4030 4088 4894 Size: 219 1837 465 327 136 220 58 806 Cuts at: 4894 6029 6710 6901 826 Size: 1135 681 191 1471 Fragments arranged by size: 1837 1471 1135 806 681 465 327 220 219 191 136 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7546 SmaI CCC'GGG Cuts at: 1872 4568 4873 1872 Size: 2696 305 4545 Fragments arranged by size: 4545 2696 305 SmlI C'TyrA_G Cuts at: 820 1017 1943 3305 4532 5650 6551 6813 820 Size: 197 926 1362 1227 1118 901 262 1553 Fragments arranged by size: 1553 1362 1227 1118 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5337 493 Size: 4844 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7546 SphI G_CATG'C Cuts at: 1132 4904 1132 Size: 3772 3774 SspI AAT'ATT Cuts at: 5 52 5470 5 Size: 47 5418 2081 Fragments arranged by size: 5418 2081 47 StuI AGG'CCT Cuts at: 1517 4094 1517 Size: 2577 4969 StyI C'CwwG_G Cuts at: 513 1065 4441 4572 4985 513 Size: 552 3376 131 413 3074 Fragments arranged by size: 3376 3074 552 413 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5573 5590 5743 5928 6373 325 Size: 1255 3993 17 153 185 445 1498 Fragments arranged by size: 3993 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3972 5792 7504 Size: 274 80 33 51 1443 1995 1820 1712 Cuts at: 7504 96 Size: 138 Fragments arranged by size: 1995 1820 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2188 2220 2616 2648 3367 3399 4814 4846 7506 Size: 32 396 32 719 32 1415 32 2660 Cuts at: 7506 7538 2188 Size: 32 2196 Fragments arranged by size: 2660 2196 1415 719 396 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3107 1316 Size: 1791 5755 XbaI T'CTAG_A Cuts at: 4882 4882 Size: 7546 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7546 XmaI C'CCGG_G Cuts at: 1870 4566 4871 1870 Size: 2696 305 4545 Fragments arranged by size: 4545 2696 305 XmnI GAAnn'nnTTC Cuts at: 4634 5675 4634 Size: 1041 6505 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3277 5354 276 Size: 53 83 186 936 1743 2077 2468 Fragments arranged by size: 2468 2077 1743 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI