################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspI AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BssHII Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT22I FseI HgaI HincII HindII HindIII HpaI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfuI SmaI SmlI SnaBI SpeI Sse8387I StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AfaI 918 GTAC GT^AC AhdI 299 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 231 GTGCAC G^TGCAC ApaLI 231 GTGCAC G^TGCAC AspEI 299 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 67 CTCGGG C^[CT]CG[GA]G AviII 248 TGCGCA TGC^GCA BanII 377 GGGCTC G[GA]GC[CT]^C BciVI 750 GGATAC GTATCC(6/5) BsoBI 67 CTCGGG C^[CT]CG[GA]G Bsp1407I 917 TGTACA T^GTACA BspHI 641 TCATGA T^CATGA BsrBI 631 CCGCTC CCGCTC(-3/-3) BsrDI 482 GCAATG GCAATG(2/0) BsrGI 917 TGTACA T^GTACA BssSI 331 CTCGTG CACGAG(-5/-1) DrdI 838 GACACTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 299 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 879 GAAGAG CTCTTC(1/4) EcoNI 53 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 239 CCATGG C^C[AT][AT]GG FspI 248 TGCGCA TGC^GCA HinfI 845 GAGTC G^A[ACGT]TC Ksp632I 879 GAAGAG CTCTTC(1/4) MslI 234 CACGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 143 GCCGGC GCC^GGC NcoI 239 CCATGG C^CATGG NgoMIV 143 GCCGGC G^CCGGC NspI 802 GCATGC [GA]CATG^[CT] PleI 845 GAGTC GAGTC(4/5) PvuII 394 CAGCTG CAG^CTG RcaI 641 TCATGA T^CATGA RsaI 918 GTAC GT^AC SapI 879 GAAGAGC GCTCTTC(1/4) SfiI 90 GGCCGCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 142 CGCCGGCG C[GA]^CCGG[CT]G SphI 802 GCATGC GCATG^C SspBI 917 TGTACA T^GTACA SspI 715 AATATT AAT^ATT StyI 239 CCATGG C^C[AT][AT]GG XcmI 43 CCAGCCCGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AcsI 600 GAATTT [GA]^AATT[CT] 702 GAATTT AcyI 119 GGCGCC G[GA]^CG[CT]C 140 GGCGCC ApoI 600 GAATTT [GA]^AATT[CT] 702 GAATTT AspHI 231 GTGCAC G[AT]GC[AT]^C 334 GTGCTC AvaII 390 GGACC G^G[AT]CC 831 GGACC BbeI 119 GGCGCC GGCGC^C 140 GGCGCC BclI 594 TGATCA T^GATCA 638 TGATCA BcnI 47 CCCGG CC^[GC]GG 189 CCCGG BfaI 82 CTAG C^TAG 407 CTAG BpmI 380 CTCCAG CTGGAG(16/14) 842 CTGGAG BsaHI 119 GGCGCC G[GA]^CG[CT]C 140 GGCGCC BseRI 15 CTCCTC GAGGAG(10/8) 745 GAGGAG BsiHKAI 231 GTGCAC G[AT]GC[AT]^C 334 GTGCTC BsmFI 106 GGGAC GGGAC(10/14) 266 GGGAC BspMI 260 ACCTGC ACCTGC(4/8) 156 GCAGGT BstEII 723 GGTGACC G^GT[ACGT]ACC 982 GGTGACC BstPI 723 GGTGACC G^GT[ACGT]ACC 982 GGTGACC BstYI 182 GGATCT [GA]^GATC[CT] 941 GGATCT EaeI 89 TGGCCG [CT]^GGCC[GA] 236 CGGCCA EcoO65I 723 GGTGACC G^GT[ACGT]ACC 982 GGTGACC FbaI 594 TGATCA T^GATCA 638 TGATCA Hin1I 119 GGCGCC G[GA]^CG[CT]C 140 GGCGCC KasI 119 GGCGCC G^GCGCC 140 GGCGCC MaeI 82 CTAG C^TAG 407 CTAG MflI 182 GGATCT [GA]^GATC[CT] 941 GGATCT MseI 287 TTAA T^TAA 464 TTAA MspA1I 252 CAGCGG C[AC]G^C[GT]G 394 CAGCTG NarI 119 GGCGCC GG^CGCC 140 GGCGCC NciI 47 CCCGG CC^[GC]GG 189 CCCGG PflMI 835 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 931 CCACTTTTTGG PstI 151 CTGCAG CTGCA^G 441 CTGCAG SfaNI 649 GATGC GCATC(5/9) 736 GATGC SfcI 151 CTGCAG C^T[GA][CT]AG 441 CTGCAG SfoI 119 GGCGCC GGC^GCC 140 GGCGCC Tru9I 287 TTAA T^TAA 464 TTAA Van91I 835 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 931 CCACTTTTTGG XhoII 182 GGATCT [GA]^GATC[CT] 941 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI BanI BbvI BglI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I Tsp509I TspRI