(Circular) (Six-base) MAPSORT of: pFN21AE0466.seq Check: 9458 from: 1 to: 5710 pFN21AE0466 5710 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2152 2152 Size: 5710 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2362 2547 2903 3773 5157 5425 1632 Size: 730 185 356 870 1384 268 1917 Fragments arranged by size: 1917 1384 870 730 356 268 185 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 2309 4439 2309 Size: 2130 3580 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2231 1184 Size: 1047 4663 AlwNI CAG_nnn'CTG Cuts at: 2481 2846 2872 3453 5025 2481 Size: 365 26 581 1572 3166 Fragments arranged by size: 3166 1572 581 365 26 ApaLI G'TGCA_C Cuts at: 2236 3767 4923 2236 Size: 1531 1156 3023 Fragments arranged by size: 3023 1531 1156 ApoI r'AATT_y Cuts at: 1493 2605 2707 3019 3245 3397 5506 1493 Size: 1112 102 312 226 152 2109 1697 Fragments arranged by size: 2109 1697 1112 312 226 152 102 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2072 3035 1870 Size: 73 129 963 4545 Fragments arranged by size: 4545 963 129 73 BamHI G'GATC_C Cuts at: 3040 3407 3040 Size: 367 5343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2124 2145 2168 2215 3031 Size: 325 217 627 337 21 23 47 816 Cuts at: 3031 4486 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 816 627 337 325 217 47 23 21 BanII G_rGCy'C Cuts at: 1391 2386 3029 1391 Size: 995 643 4072 Fragments arranged by size: 4072 995 643 BbeI G_GCGC'C Cuts at: 2128 2149 2128 Size: 21 5689 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2749 3602 4812 2749 Size: 853 1210 3647 Fragments arranged by size: 3647 1210 853 BclI T'GATC_A Cuts at: 1548 2599 2643 1548 Size: 1051 44 4615 Fragments arranged by size: 4615 1051 44 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2068 2102 2143 Size: 107 122 71 631 605 396 34 41 Cuts at: 2143 4321 136 Size: 2178 1525 Fragments arranged by size: 2178 1525 631 605 396 122 107 71 41 34 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 2171 2240 3771 4927 1790 Size: 381 69 1531 1156 2573 Fragments arranged by size: 2573 1531 1156 381 69 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2370 2868 4370 1239 Size: 1131 498 1502 2579 Fragments arranged by size: 2579 1502 1131 498 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4373 882 Size: 617 2874 2219 Fragments arranged by size: 2874 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2125 2146 3517 3899 Size: 53 83 186 936 592 21 1371 382 Cuts at: 3899 4534 275 Size: 635 1451 Fragments arranged by size: 1451 1371 936 635 592 382 186 83 53 21 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2011 2765 1186 Size: 825 754 4131 Fragments arranged by size: 4131 825 754 BseYI C'CCAG_C Cuts at: 2047 4913 5409 2047 Size: 2866 496 2348 Fragments arranged by size: 2866 2348 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5137 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2240 2343 3771 3856 4927 2240 Size: 103 1428 85 1071 3023 Fragments arranged by size: 3023 1428 1071 103 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2171 2240 2343 2386 3029 3771 3856 Size: 399 381 69 103 43 643 742 85 Cuts at: 3856 4927 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 643 399 381 103 85 69 43 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 2646 3599 5329 2646 Size: 953 1730 3027 Fragments arranged by size: 3027 1730 953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2152 2274 3065 844 Size: 1308 122 791 3489 Fragments arranged by size: 3489 1308 791 122 BsrBI CCG'CTC Cuts at: 1394 1823 2638 3597 1394 Size: 429 815 959 3507 Fragments arranged by size: 3507 959 815 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2494 4205 4379 65 Size: 1183 1246 1711 174 1396 Fragments arranged by size: 1711 1396 1246 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2148 2218 2732 4354 4541 1700 Size: 110 338 70 514 1622 187 2869 Fragments arranged by size: 2869 1622 514 338 187 110 70 BsrGI T'GTAC_A Cuts at: 96 2922 96 Size: 2826 2884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 2336 3770 4782 2336 Size: 1434 1012 3264 Fragments arranged by size: 3264 1434 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 2728 2987 1184 Size: 1544 259 3907 Fragments arranged by size: 3907 1544 259 BstYI r'GATC_y Cuts at: 1972 2187 2946 3040 3407 3792 3809 5261 5347 Size: 215 759 94 367 385 17 1452 86 Cuts at: 5347 5359 5705 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 759 385 367 346 215 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2100 2172 2244 513 Size: 552 939 96 72 72 3979 Fragments arranged by size: 3979 939 552 96 72 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 2313 3226 4020 4040 2313 Size: 913 794 20 3983 Fragments arranged by size: 3983 913 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2706 2741 529 Size: 35 2142 35 3498 Fragments arranged by size: 3498 2142 35 35 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2849 4717 809 Size: 507 1533 1868 1802 Fragments arranged by size: 1868 1802 1533 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2241 3157 Size: 54 1425 50 275 18 264 147 916 Cuts at: 3157 4046 5675 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 916 889 275 264 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2879 3640 1353 Size: 1526 761 3423 Fragments arranged by size: 3423 1526 761 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2362 2370 2547 2868 2903 3773 4370 Size: 393 730 8 177 321 35 870 597 Cuts at: 4370 5157 5425 1239 Size: 787 268 1524 Fragments arranged by size: 1524 870 787 730 597 393 321 268 177 35 8 EcoNI CCTnn'n_nnAGG Cuts at: 2062 2062 Size: 5710 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 2255 4216 2255 Size: 1961 3749 HaeII r_GCGC'y Cuts at: 1678 2013 2128 2149 4857 1678 Size: 335 115 21 2708 2531 Fragments arranged by size: 2708 2531 335 115 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2295 2327 4351 4383 4425 Size: 32 135 32 723 32 2024 32 42 Cuts at: 4425 4457 1373 Size: 32 2626 Fragments arranged by size: 2626 2024 723 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KasI G'GCGC_C Cuts at: 2124 2145 2124 Size: 21 5689 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4824 5008 1305 Size: 3519 184 2007 Fragments arranged by size: 3519 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 3668 4027 4186 518 Size: 648 1077 1425 359 159 2042 Fragments arranged by size: 2042 1425 1077 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2259 2401 3126 3803 4951 5196 1606 Size: 653 142 725 677 1148 245 2120 Fragments arranged by size: 2120 1148 725 677 653 245 142 NaeI GCC'GGC Cuts at: 2150 2150 Size: 5710 NarI GG'CG_CC Cuts at: 2125 2146 2125 Size: 21 5689 NcoI C'CATG_G Cuts at: 513 1065 2004 2244 513 Size: 552 939 240 3979 Fragments arranged by size: 3979 939 552 240 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NgoMIV G'CCGG_C Cuts at: 2148 2148 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 2811 3068 4613 1132 Size: 97 1582 257 1545 2229 Fragments arranged by size: 2229 1582 1545 257 97 PasI CC'CwG_GG Cuts at: 1348 2107 1348 Size: 759 4951 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 2846 2942 1108 Size: 1738 96 3876 Fragments arranged by size: 3876 1738 96 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PstI C_TGCA'G Cuts at: 830 2160 2450 3062 830 Size: 1330 290 612 3478 Fragments arranged by size: 3478 1330 612 290 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 PvuII CAG'CTG Cuts at: 2401 2401 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SapI GCTCTTCn'nnn_ Cuts at: 2879 2879 Size: 5710 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2156 2446 3058 4193 4874 5065 826 Size: 219 1111 290 612 1135 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 612 290 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2102 2102 Size: 5710 SfoI GGC'GCC Cuts at: 2126 2147 2126 Size: 21 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 2148 1700 Size: 448 5262 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3814 4715 4977 820 Size: 197 926 1871 901 262 1553 Fragments arranged by size: 1871 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 2811 3068 1132 Size: 1679 257 3774 Fragments arranged by size: 3774 1679 257 SspI AAT'ATT Cuts at: 5 52 2722 3634 5 Size: 47 2670 912 2081 Fragments arranged by size: 2670 2081 912 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 2244 3149 513 Size: 552 939 240 905 3074 Fragments arranged by size: 3074 939 905 552 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3737 3754 3907 4092 4537 325 Size: 1255 2157 17 153 185 445 1498 Fragments arranged by size: 2157 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2922 3956 5668 Size: 274 80 33 51 1443 945 1034 1712 Cuts at: 5668 96 Size: 138 Fragments arranged by size: 1712 1443 1034 945 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2055 2055 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SrfI SwaI