Restriction map for
pFN21AA0136
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AviII AvrII BamHI BanI
BanII BbeI BbrPI BcnI BglI
BglII BlnI BmyI BpmI BsaHI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I Bsp1407I BspDI BspEI
BsrBI BsrDI BsrFI BsrGI BssSI
Bst1107I BstEII BstPI BstXI BstYI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoT14I EcoT22I FseI FspI HaeII
Hin1I KasI KpnI MfeI MflI
MluI MroI MunI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflII 1054 CTTAAG C^TTAAG
AflIII 2373 ACATGT A^C[GA][CT]GT
AlwNI 95 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 664 CCCGAG C^[CT]CG[GA]G
BbvCI 30 CCTCAGC CCTCAGC(-5/-2)
BcgI 2055 CGATAAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1054 CTTAAG C^TTAAG
BlpI 240 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 240 GCTAAGC GC^T[ACGT]AGC
BsaAI 803 TACGTG [CT]AC^GT[GA]
BsaWI 1434 ACCGGA [AT]^CCGG[AT]
BseRI 2635 GAGGAG GAGGAG(10/8)
BsoBI 664 CCCGAG C^[CT]CG[GA]G
BspHI 1773 TCATGA T^CATGA
BspLU11I 2373 ACATGT A^CATGT
BspMI 1761 ACCTGC ACCTGC(4/8)
BssHII 35 GCGCGC G^CGCGC
BstAPI 2065 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 834 TTCGAA TT^CGAA
CelII 240 GCTAAGC GC^T[ACGT]AGC
DraIII 2546 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 659 GAATTC G^AATTC
EcoRV 652 GATATC GAT^ATC
HapII 1435 CCGG C^CGG
HincII 2689 GTTAAC GT[CT]^[GA]AC
HindII 2689 GTTAAC GT[CT]^[GA]AC
HpaI 2689 GTTAAC GTT^AAC
HpaII 1435 CCGG C^CGG
KspI 18 CCGCGG CCGC^GG
MspI 1435 CCGG C^CGG
NspV 834 TTCGAA TT^CGAA
PvuII 826 CAGCTG CAG^CTG
RcaI 1773 TCATGA T^CATGA
SacII 18 CCGCGG CCGC^GG
ScaI 2812 AGTACT AGT^ACT
SfuI 834 TTCGAA TT^CGAA
SmlI 1054 CTTAAG C^T[CT][GA]AG
SpeI 70 ACTAGT A^CTAGT
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 19 CGCG CG^CG
36 CGCG
AocI 750 CCTGAGG CC^T[ACGT]AGG
1510 CCTAAGG
AspI 259 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2144 GACAGTGTC
AvaII 720 GGACC G^G[AT]CC
2490 GGACC
BalI 82 TGGCCA TGG^CCA
1857 TGGCCA
BciVI 23 GGATAC GTATCC(6/5)
2189 GGATAC
BclI 1218 TGATCA T^GATCA
2163 TGATCA
BsaBI 862 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2725 GATCTTGATC
BsgI 92 GTGCAG GTGCAG(16/14)
2440 GTGCAG
BsiYI 13 CCACCCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
197 CCGACAATGGG
BslI 13 CCACCCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
197 CCGACAATGGG
BsmAI 1629 GAGAC GTCTC(1/5)
390 GTCTC
BstUI 19 CGCG CG^CG
36 CGCG
Bsu36I 750 CCTGAGG CC^T[ACGT]AGG
1510 CCTAAGG
DsaI 18 CCGCGG C^C[GA][CT]GG
527 CCACGG
EarI 2681 CTCTTC CTCTTC(1/4)
837 GAAGAG
Eco81I 750 CCTGAGG CC^T[ACGT]AGG
1510 CCTAAGG
FauI 17 CCCGC CCCGC(4/6)
20 GCGGG
FbaI 1218 TGATCA T^GATCA
2163 TGATCA
Ksp632I 2681 CTCTTC CTCTTC(1/4)
837 GAAGAG
MamI 862 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2725 GATCTTGATC
MluNI 82 TGGCCA TGG^CCA
1857 TGGCCA
MscI 82 TGGCCA TGG^CCA
1857 TGGCCA
MspA1I 18 CCGCGG C[AC]G^C[GT]G
826 CAGCTG
MvnI 19 CGCG CG^CG
36 CGCG
NlaIV 1341 GGTTCC GG[ACGT]^[ACGT]CC
2489 GGGACC
NspI 423 GCATGT [GA]CATG^[CT]
2373 ACATGT
PflFI 259 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2144 GACAGTGTC
Tth111I 259 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2144 GACAGTGTC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I BbsI
BbvI BfaI BpuAI BsaJI BsmFI
BsmI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HindIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeI NdeII NlaIII
PleI PshBI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI