(Circular) (Six-base) MAPSORT of: pFN21AA0136.seq Check: 2874 from: 1 to: 7522 pFN21AA0136 7522 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5332 278 Size: 53 83 186 936 3796 2468 Fragments arranged by size: 3796 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4865 1937 Size: 2928 4594 Acc65I G'GTAC_C Cuts at: 4843 4843 Size: 7522 AclI AA'CG_TT Cuts at: 1563 5649 6022 1563 Size: 4086 373 3063 Fragments arranged by size: 4086 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2242 3381 3951 4047 4593 4724 5585 6969 Size: 610 1139 570 96 546 131 861 1384 Cuts at: 6969 7237 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1139 861 610 570 546 268 131 96 AflII C'TTAA_G Cuts at: 820 1017 3059 820 Size: 197 2042 5283 Fragments arranged by size: 5283 2042 197 AflIII A'CryG_T Cuts at: 1203 4378 6421 7398 1203 Size: 3175 2043 977 1327 Fragments arranged by size: 3175 2043 1327 977 AgeI A'CCGG_T Cuts at: 6353 6353 Size: 7522 AhdI GACnn_n'nnGTC Cuts at: 6251 6251 Size: 7522 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7522 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7522 AlwNI CAG_nnn'CTG Cuts at: 2105 5265 6837 2105 Size: 3160 1572 2790 Fragments arranged by size: 3160 2790 1572 ApaLI G'TGCA_C Cuts at: 5579 6735 5579 Size: 1156 6366 ApoI r'AATT_y Cuts at: 1493 2068 2664 2882 3190 3422 3556 3697 4831 Size: 575 596 218 308 232 134 141 1134 Cuts at: 4831 5057 5209 7318 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1134 596 575 308 232 226 218 152 141 134 AseI AT'TA_AT Cuts at: 160 1032 2111 2475 3459 4281 6076 160 Size: 872 1079 364 984 822 1795 1606 Fragments arranged by size: 1795 1606 1079 984 872 822 364 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7522 AvaI C'yCGr_G Cuts at: 1870 1943 2669 4847 1870 Size: 73 726 2178 4545 Fragments arranged by size: 4545 2178 726 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3918 3951 3918 Size: 33 7489 BamHI G'GATC_C Cuts at: 4852 5219 4852 Size: 367 7155 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4843 6298 618 Size: 325 217 627 3056 1455 1842 Fragments arranged by size: 3056 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4841 1391 Size: 3450 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2831 3541 3978 928 Size: 971 932 710 437 4472 Fragments arranged by size: 4472 971 932 710 437 BbvCI CC'TCA_GC Cuts at: 2036 2036 Size: 7522 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4049 4083 5713 5747 1903 Size: 34 2112 34 1630 34 3678 Fragments arranged by size: 3678 2112 1630 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2022 4188 5414 6624 2022 Size: 2166 1226 1210 2920 Fragments arranged by size: 2920 2166 1226 1210 BclI T'GATC_A Cuts at: 1548 3223 4168 1548 Size: 1675 945 4902 Fragments arranged by size: 4902 1675 945 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 6133 136 Size: 107 122 71 631 605 337 4124 1525 Fragments arranged by size: 4124 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7517 7517 Size: 7522 BlpI GC'TnA_GC Cuts at: 2246 4939 2246 Size: 2693 4829 Bme1580I G_kGCm'C Cuts at: 1790 5583 6739 1790 Size: 3793 1156 2573 Fragments arranged by size: 3793 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7522 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2285 5253 6211 Size: 416 53 83 399 24 868 2968 958 Cuts at: 6211 442 Size: 1753 Fragments arranged by size: 2968 1753 958 868 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7522 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4688 4688 Size: 7522 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6182 1239 Size: 4943 2579 Bpu10I CC'TnA_GC Cuts at: 2036 2036 Size: 7522 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5647 6512 6810 5647 Size: 865 298 6359 Fragments arranged by size: 6359 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6185 882 Size: 617 4686 2219 Fragments arranged by size: 4686 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2810 5313 493 Size: 711 1606 2503 2702 Fragments arranged by size: 2702 2503 1606 711 BsaBI GATnn'nnATC Cuts at: 2871 4734 7516 2871 Size: 1863 2782 2877 Fragments arranged by size: 2877 2782 1863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5329 5711 6346 275 Size: 53 83 186 936 3796 382 635 1451 Fragments arranged by size: 3796 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3439 5955 6353 6627 6774 7185 3439 Size: 2516 398 274 147 411 3776 Fragments arranged by size: 3776 2516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3667 3697 1178 Size: 30 165 30 2264 30 5003 Fragments arranged by size: 5003 2264 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4655 1186 Size: 3469 4053 BseYI C'CCAG_C Cuts at: 6725 7221 6725 Size: 496 7026 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2118 4466 1163 Size: 573 382 2348 4219 Fragments arranged by size: 4219 2348 573 382 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4972 5733 6761 1540 Size: 275 186 2971 761 1028 2301 Fragments arranged by size: 2971 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5583 5668 6739 5583 Size: 85 1071 6366 Fragments arranged by size: 6366 1071 85 BsmI GAATG_Cn' Cuts at: 2450 2799 3667 4397 5033 5126 2450 Size: 349 868 730 636 93 4846 Fragments arranged by size: 4846 868 730 636 349 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4841 5583 5668 6739 1391 Size: 399 3051 742 85 1071 2174 Fragments arranged by size: 3051 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7185 7185 Size: 7522 BspHI T'CATG_A Cuts at: 3778 5411 7141 3778 Size: 1633 1730 4159 Fragments arranged by size: 4159 1730 1633 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3775 4877 844 Size: 2931 1102 3489 Fragments arranged by size: 3489 2931 1102 BsrBI CCG'CTC Cuts at: 1394 1823 5409 1394 Size: 429 3586 3507 Fragments arranged by size: 3586 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6017 6191 65 Size: 1183 4769 174 1396 Fragments arranged by size: 4769 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6166 6353 1700 Size: 110 4356 187 2869 Fragments arranged by size: 4356 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7522 BssHII G'CGCG_C Cuts at: 1925 2040 1925 Size: 115 7407 BssSI C'ACGA_G Cuts at: 5582 6594 5582 Size: 1012 6510 BstAPI GCAn_nnn'nTGC Cuts at: 794 4076 794 Size: 3282 4240 BstBI TT'CG_AA Cuts at: 2840 6326 2840 Size: 3486 4036 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7522 BstYI r'GATC_y Cuts at: 1972 4852 5219 5604 5621 7073 7159 7171 7517 Size: 2880 367 385 17 1452 86 12 346 Cuts at: 7517 1972 Size: 1977 Fragments arranged by size: 2880 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2756 3516 2756 Size: 760 6762 BtgI C'CryG_G Cuts at: 513 1065 2004 2023 2532 513 Size: 552 939 19 509 5503 Fragments arranged by size: 5503 939 552 509 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6783 Fragments arranged by size: 6783 683 56 BtsI GCAGTG_nn' Cuts at: 4005 5038 5832 5852 4005 Size: 1033 794 20 5675 Fragments arranged by size: 5675 1033 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7522 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7487 DraI TTT'AAA Cuts at: 4826 4826 Size: 7522 DraIII CAC_nnn'GTG Cuts at: 4556 4556 Size: 7522 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6529 809 Size: 507 5213 1802 Fragments arranged by size: 5213 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2087 3479 3862 Size: 54 1425 50 275 18 257 1392 383 Cuts at: 3862 4969 5858 7487 8 Size: 1107 889 1629 43 Fragments arranged by size: 1629 1425 1392 1107 889 383 275 257 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4969 1537 Size: 275 3157 4090 Fragments arranged by size: 4090 3157 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2837 4692 5452 1353 Size: 1484 1855 760 3423 Fragments arranged by size: 3423 1855 1484 760 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3277 6109 6483 6629 387 Size: 1064 138 1688 2832 374 146 1280 Fragments arranged by size: 2832 1688 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2242 3381 3951 4047 4593 4724 5585 Size: 393 610 1139 570 96 546 131 861 Cuts at: 5585 6182 6969 7237 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1139 861 787 610 597 570 546 393 268 131 96 EcoRI G'AATT_C Cuts at: 2664 4831 5209 2664 Size: 2167 378 4977 Fragments arranged by size: 4977 2167 378 EcoRV GAT'ATC Cuts at: 2659 2659 Size: 7522 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2516 3525 2516 Size: 1009 6513 FspI TGC'GCA Cuts at: 6028 6028 Size: 7522 HaeII r_GCGC'y Cuts at: 1678 6669 1678 Size: 4991 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3779 3811 6163 6195 6237 Size: 32 135 32 2207 32 2352 32 42 Cuts at: 6237 6269 1373 Size: 32 2626 Fragments arranged by size: 2626 2352 2207 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4696 4866 5112 669 Size: 988 281 2758 170 246 3079 Fragments arranged by size: 3079 2758 988 281 246 170 HindIII A'AGCT_T Cuts at: 748 2248 2979 3502 4329 748 Size: 1500 731 523 827 3941 Fragments arranged by size: 3941 1500 827 731 523 HpaI GTT'AAC Cuts at: 4696 5112 4696 Size: 416 7106 KpnI G_GTAC'C Cuts at: 4847 4847 Size: 7522 MfeI C'AATT_G Cuts at: 5121 5121 Size: 7522 MluI A'CGCG_T Cuts at: 7398 7398 Size: 7522 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2275 2974 3007 3016 6636 6820 1305 Size: 970 699 33 9 3620 184 2007 Fragments arranged by size: 3620 2007 970 699 184 33 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 2089 3864 10 Size: 54 1425 343 257 1775 3668 Fragments arranged by size: 3668 1775 1425 343 257 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 3895 4659 5480 5839 5998 518 Size: 648 1215 1514 764 821 359 159 2042 Fragments arranged by size: 2042 1514 1215 821 764 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2025 2833 4938 5615 6763 7008 1606 Size: 419 808 2105 677 1148 245 2120 Fragments arranged by size: 2120 2105 1148 808 677 419 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6031 Fragments arranged by size: 6031 939 552 NdeI CA'TA_TG Cuts at: 387 4158 4308 4656 387 Size: 3771 150 348 3253 Fragments arranged by size: 3771 3253 348 150 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7522 NotI GC'GGCC_GC Cuts at: 4969 4969 Size: 7522 NspI r_CATG'y Cuts at: 1132 1229 2432 4382 4880 6425 1132 Size: 97 1203 1950 498 1545 2229 Fragments arranged by size: 2229 1950 1545 1203 498 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7522 PciI A'CATG_T Cuts at: 4378 6421 4378 Size: 2043 5479 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7522 PmeI GTTT'AAAC Cuts at: 4826 4826 Size: 7522 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5591 5623 7104 7136 5591 Size: 32 1481 32 5977 Fragments arranged by size: 5977 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7522 PsiI TTA'TAA Cuts at: 3708 4143 5092 3708 Size: 435 949 6138 Fragments arranged by size: 6138 949 435 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2529 2561 2529 Size: 32 7490 PstI C_TGCA'G Cuts at: 830 2927 3772 3919 4072 4874 830 Size: 2097 845 147 153 802 3478 Fragments arranged by size: 3478 2097 845 802 153 147 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7522 PvuII CAG'CTG Cuts at: 2833 2833 Size: 7522 SacII CC_GC'GG Cuts at: 2026 2026 Size: 7522 SalI G'TCGA_C Cuts at: 1936 4864 1936 Size: 2928 4594 SbfI CC_TGCA'GG Cuts at: 4874 4874 Size: 7522 ScaI AGT'ACT Cuts at: 4819 5770 7482 4819 Size: 951 1712 4859 Fragments arranged by size: 4859 1712 951 SfcI C'TryA_G Cuts at: 826 1045 2923 3768 3915 4068 4870 6005 6686 Size: 219 1878 845 147 153 802 1135 681 Cuts at: 6686 6877 826 Size: 191 1471 Fragments arranged by size: 1878 1471 1135 845 802 681 219 191 153 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7522 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7522 SmaI CCC'GGG Cuts at: 1872 4849 1872 Size: 2977 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3059 5626 6527 6789 820 Size: 197 926 1116 2567 901 262 1553 Fragments arranged by size: 2567 1553 1116 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5313 493 Size: 4820 2702 SpeI A'CTAG_T Cuts at: 152 2075 152 Size: 1923 5599 SphI G_CATG'C Cuts at: 1132 4880 1132 Size: 3748 3774 SspI AAT'ATT Cuts at: 5 52 5446 5 Size: 47 5394 2081 Fragments arranged by size: 5394 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7522 StyI C'CwwG_G Cuts at: 513 1065 2004 4961 513 Size: 552 939 2957 3074 Fragments arranged by size: 3074 2957 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3011 3833 5549 5566 5719 5904 6349 Size: 1255 1431 822 1716 17 153 185 445 Cuts at: 6349 325 Size: 1498 Fragments arranged by size: 1716 1498 1431 1255 822 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3102 4817 5768 Size: 274 80 33 51 1443 1125 1715 951 Cuts at: 5768 7480 96 Size: 1712 138 Fragments arranged by size: 1715 1712 1443 1125 951 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2805 2837 7482 7514 2805 Size: 32 4645 32 2813 Fragments arranged by size: 4645 2813 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2267 4152 1316 Size: 951 1885 4686 Fragments arranged by size: 4686 1885 951 XbaI T'CTAG_A Cuts at: 4858 4858 Size: 7522 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7522 XmaI C'CCGG_G Cuts at: 1870 4847 1870 Size: 2977 4545 XmnI GAAnn'nnTTC Cuts at: 2312 3525 3556 5651 2312 Size: 1213 31 2095 4183 Fragments arranged by size: 4183 2095 1213 31 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5330 276 Size: 53 83 186 936 3796 2468 Fragments arranged by size: 3796 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI