Restriction map for
pFN21AA0136
(Linear) (Six-base) MAPSORT of: KIAA0136.seq Check: 3213 from: 1 to: 2817
KIAA0136.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 1376 1946 2042 2588 2719 2817
Size: 237 1139 570 96 546 131 98
Fragments arranged by size:
1139 570 546 237 131 98 96
AflII C'TTAA_G
Cuts at: 0 1054 2817
Size: 1054 1763
AflIII A'CryG_T
Cuts at: 0 2373 2817
Size: 2373 444
AlwNI CAG_nnn'CTG
Cuts at: 0 100 2817
Size: 100 2717
ApoI r'AATT_y
Cuts at: 0 63 659 877 1185 1417 1551 1692 2817
Size: 63 596 218 308 232 134 141 1125
Fragments arranged by size:
1125 596 308 232 218 141 134 63
AseI AT'TA_AT
Cuts at: 0 106 470 1454 2276 2817
Size: 106 364 984 822 541
Fragments arranged by size:
984 822 541 364 106
AvaI C'yCGr_G
Cuts at: 0 664 2817
Size: 664 2153
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1913 1946 2817
Size: 1913 33 871
Fragments arranged by size:
1913 871 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 826 1536 1973 2817
Size: 826 710 437 844
Fragments arranged by size:
844 826 710 437
BbvCI CC'TCA_GC
Cuts at: 0 31 2817
Size: 31 2786
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2044 2078 2817
Size: 2044 34 739
Fragments arranged by size:
2044 739 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 17 2183 2817
Size: 17 2166 634
Fragments arranged by size:
2166 634 17
BclI T'GATC_A
Cuts at: 0 1218 2163 2817
Size: 1218 945 654
Fragments arranged by size:
1218 945 654
BlpI GC'TnA_GC
Cuts at: 0 241 2817
Size: 241 2576
BmrI ACTGGGnnnn_n'
Cuts at: 0 280 2817
Size: 280 2537
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2683 2817
Size: 2683 134
Bpu10I CC'TnA_GC
Cuts at: 0 31 2817
Size: 31 2786
BsaAI yAC'GTr
Cuts at: 0 805 2817
Size: 805 2012
BsaBI GATnn'nnATC
Cuts at: 0 866 2729 2817
Size: 866 1863 88
Fragments arranged by size:
1863 866 88
BsaWI w'CCGG_w
Cuts at: 0 1434 2817
Size: 1434 1383
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1662 1692 2817
Size: 1662 30 1125
Fragments arranged by size:
1662 1125 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2650 2817
Size: 2650 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 2461 2817
Size: 113 2348 356
Fragments arranged by size:
2348 356 113
BsmI GAATG_Cn'
Cuts at: 0 445 794 1662 2392 2817
Size: 445 349 868 730 425
Fragments arranged by size:
868 730 445 425 349
BspHI T'CATG_A
Cuts at: 0 1773 2817
Size: 1773 1044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1770 2817
Size: 1770 1047
BssHII G'CGCG_C
Cuts at: 0 35 2817
Size: 35 2782
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2071 2817
Size: 2071 746
BstBI TT'CG_AA
Cuts at: 0 835 2817
Size: 835 1982
Bsu36I CC'TnA_GG
Cuts at: 0 751 1511 2817
Size: 751 760 1306
Fragments arranged by size:
1306 760 751
BtgI C'CryG_G
Cuts at: 0 18 527 2817
Size: 18 509 2290
Fragments arranged by size:
2290 509 18
BtsI GCAGTG_nn'
Cuts at: 0 2000 2817
Size: 2000 817
DraIII CAC_nnn'GTG
Cuts at: 0 2551 2817
Size: 2551 266
EaeI y'GGCC_r
Cuts at: 0 82 1474 1857 2817
Size: 82 1392 383 960
Fragments arranged by size:
1392 960 383 82
EarI CTCTTCn'nnn_
Cuts at: 0 832 2687 2817
Size: 832 1855 130
Fragments arranged by size:
1855 832 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1272 2817
Size: 1272 1545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 1376 1946 2042 2588 2719 2817
Size: 237 1139 570 96 546 131 98
Fragments arranged by size:
1139 570 546 237 131 98 96
EcoRI G'AATT_C
Cuts at: 0 659 2817
Size: 659 2158
EcoRV GAT'ATC
Cuts at: 0 654 2817
Size: 654 2163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 511 1520 2817
Size: 511 1009 1297
Fragments arranged by size:
1297 1009 511
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1774 1806 2817
Size: 1774 32 1011
Fragments arranged by size:
1774 1011 32
HincII GTy'rAC
Cuts at: 0 2691 2817
Size: 2691 126
HindIII A'AGCT_T
Cuts at: 0 243 974 1497 2324 2817
Size: 243 731 523 827 493
Fragments arranged by size:
827 731 523 493 243
HpaI GTT'AAC
Cuts at: 0 2691 2817
Size: 2691 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 969 1002 1011 2817
Size: 270 699 33 9 1806
Fragments arranged by size:
1806 699 270 33 9
MscI TGG'CCA
Cuts at: 0 84 1859 2817
Size: 84 1775 958
Fragments arranged by size:
1775 958 84
MslI CAynn'nnrTG
Cuts at: 0 376 1890 2654 2817
Size: 376 1514 764 163
Fragments arranged by size:
1514 764 376 163
MspA1I CmG'CkG
Cuts at: 0 20 828 2817
Size: 20 808 1989
Fragments arranged by size:
1989 808 20
NdeI CA'TA_TG
Cuts at: 0 2153 2303 2651 2817
Size: 2153 150 348 166
Fragments arranged by size:
2153 348 166 150
NspI r_CATG'y
Cuts at: 0 427 2377 2817
Size: 427 1950 440
Fragments arranged by size:
1950 440 427
PciI A'CATG_T
Cuts at: 0 2373 2817
Size: 2373 444
PsiI TTA'TAA
Cuts at: 0 1703 2138 2817
Size: 1703 435 679
Fragments arranged by size:
1703 679 435
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 524 556 2817
Size: 524 32 2261
Fragments arranged by size:
2261 524 32
PstI C_TGCA'G
Cuts at: 0 922 1767 1914 2067 2817
Size: 922 845 147 153 750
Fragments arranged by size:
922 845 750 153 147
PvuII CAG'CTG
Cuts at: 0 828 2817
Size: 828 1989
SacII CC_GC'GG
Cuts at: 0 21 2817
Size: 21 2796
ScaI AGT'ACT
Cuts at: 0 2814 2817
Size: 2814 3
SfcI C'TryA_G
Cuts at: 0 918 1763 1910 2063 2817
Size: 918 845 147 153 754
Fragments arranged by size:
918 845 754 153 147
SmlI C'TyrA_G
Cuts at: 0 1054 2817
Size: 1054 1763
SpeI A'CTAG_T
Cuts at: 0 70 2817
Size: 70 2747
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1006 1828 2817
Size: 1006 822 989
Fragments arranged by size:
1006 989 822
TatI w'GTAC_w
Cuts at: 0 1097 2812 2817
Size: 1097 1715 5
Fragments arranged by size:
1715 1097 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 800 832 2817
Size: 800 32 1985
Fragments arranged by size:
1985 800 32
Tth111I GACn'n_nGTC
Cuts at: 0 262 2147 2817
Size: 262 1885 670
Fragments arranged by size:
1885 670 262
XmnI GAAnn'nnTTC
Cuts at: 0 307 1520 1551 2817
Size: 307 1213 31 1266
Fragments arranged by size:
1266 1213 307 31
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApoI AseI AvaI BaeI
BbsI BbvCI BcgI BciVI BclI BlpI BmrI BplI
Bpu10I BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsmI
BspHI BspMI BssHII BstAPI BstBI Bsu36I BtgI BtsI
DraIII EaeI EarI EciI Eco57MI EcoRI EcoRV FalI
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NdeI NspI PciI PsiI PsrI PstI PvuII SacII
ScaI SfcI SmlI SpeI TaqII TatI TstI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BamHI BanI BanII BbeI BfrBI BglI BglII Bme1580I
BmgBI BmtI BpmI BpuEI BsaI BsaHI BseYI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI
BsrGI BssSI BstEII BstXI BstYI BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EcoICRI EcoNI EcoO109I FseI
FspI FspAI HaeII KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PvuI RsrII SacI SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI StuI StyI SwaI XbaI XcmI
XhoI XmaI ZraI