################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbrPI BbvCI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI BsoBI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccII 12 CGCG CG^CG AcsI 868 AAATTC [GA]^AATT[CT] AcyI 135 GGCGCC G[GA]^CG[CT]C AflIII 858 ACATGT A^C[GA][CT]GT AlwI 895 GATCC GGATC(4/5) ApoI 868 AAATTC [GA]^AATT[CT] AseI 367 ATTAAT AT^TAAT AsnI 367 ATTAAT AT^TAAT Asp700I 445 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 135 GGCGCC G^G[CT][GA]CC BanII 155 GGGCTC G[GA]GC[CT]^C BbeI 135 GGCGCC GGCGC^C BbsI 449 GTCTTC GAAGAC(2/6) BcgI 51 CGAGGGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 706 AGATCT A^GATCT BmyI 155 GGGCTC G[AGT]GC[ACT]^C BpuAI 449 GTCTTC GAAGAC(2/6) BsaHI 135 GGCGCC G[GA]^CG[CT]C BsgI 107 CTGCAC GTGCAG(16/14) BsmBI 148 GAGACG CGTCTC(1/5) Bsp1286I 155 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 584 TGTACA T^GTACA BspLU11I 858 ACATGT A^CATGT BspMI 669 ACCTGC ACCTGC(4/8) BsrDI 988 GCAATG GCAATG(2/0) BsrGI 584 TGTACA T^GTACA BstF5I 996 GGATG GGATG(2/0) BstNI 207 CCTGG CC^[AT]GG BstUI 12 CGCG CG^CG BstYI 706 AGATCT [GA]^GATC[CT] EcoRII 207 CCTGG ^CC[AT]GG EcoT22I 628 ATGCAT ATGCA^T FokI 996 GGATG GGATG(9/13) Hin1I 135 GGCGCC G[GA]^CG[CT]C KasI 135 GGCGCC G^GCGCC MaeII 969 ACGT A^CGT MflI 706 AGATCT [GA]^GATC[CT] MspA1I 116 CAGCGG C[AC]G^C[GT]G MvaI 207 CCTGG CC^[AT]GG MvnI 12 CGCG CG^CG NaeI 332 GCCGGC GCC^GGC NarI 135 GGCGCC GG^CGCC NgoMIV 332 GCCGGC G^CCGGC NsiI 628 ATGCAT ATGCA^T NspI 858 ACATGT [GA]CATG^[CT] PshAI 298 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 367 ATTAAT AT^TAAT PstI 59 CTGCAG CTGCA^G SfoI 135 GGCGCC GGC^GCC SspBI 584 TGTACA T^GTACA TfiI 724 GAATC G^A[AT]TC TspRI 728 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 706 AGATCT [GA]^GATC[CT] XmnI 445 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AvaII 130 GGACC G^G[AT]CC 561 GGACC BclI 596 TGATCA T^GATCA 927 TGATCA BcnI 160 CCCGG CC^[GC]GG 176 CCCGG BsaJI 50 CCGAGG C^C[ACGT][ACGT]GG 207 CCTGGG BsmAI 148 GAGAC GTCTC(1/5) 85 GTCTC BsrFI 132 ACCGGC [GA]^CCGG[CT] 332 GCCGGC BsrI 768 ACTGG ACTGG(1/-1) 685 CCAGT Cfr10I 132 ACCGGC [GA]^CCGG[CT] 332 GCCGGC EarI 259 GAAGAG CTCTTC(1/4) 280 GAAGAG FbaI 596 TGATCA T^GATCA 927 TGATCA Ksp632I 259 GAAGAG CTCTTC(1/4) 280 GAAGAG NciI 160 CCCGG CC^[GC]GG 176 CCCGG PleI 340 GACTC GAGTC(4/5) 303 GAGTC SfcI 59 CTGCAG C^T[GA][CT]AG 492 CTACAG TaqI 230 TCGA T^CGA 831 TCGA Tsp45I 19 GTGAC ^GT[GC]AC 305 GTCAC Tsp509I 590 AATT ^AATT 869 AATT TthHB8I 230 TCGA T^CGA 831 TCGA XbaI 603 TCTAGA T^CTAGA 654 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AfaI AluI BbvI BfaI BpmI BseRI BsiYI BslI BsmFI BssKI Cac8I CfoI Cfr13I DdeI DpnII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI